RESUMO
This study aimed to identify plasma proteins that could serve as potential biomarkers for microbial invasion of the amniotic cavity (MIAC) or intra-amniotic inflammation (IAI) in women with preterm labor (PTL). A retrospective cohort comprised singleton pregnant women with PTL (24-34 weeks) who underwent amniocentesis. Pooled plasma samples were analyzed by label-free liquid chromatography-tandem mass spectrometry for proteome profiling in a nested case-control study (concomitant MIAC/IAI cases vs. non-MIAC/IAI controls [n = 10 per group]). Eight target proteins associated with MIAC/IAI were further verified by immunoassays in a large cohort (n = 230). Shotgun proteomic analysis revealed 133 differentially expressed proteins (fold change > 1.5, P < 0.05) in the plasma of MIAC/IAI cases. Further quantification confirmed that the levels of AFP were higher and those of kallistatin and TGFBI were lower in the plasma of women with MIAC and that the levels of kallistatin and TGFBI were lower in the plasma of women with IAI than in those without these conditions. The area under the curves of plasma AFP, kallistatin, and TGFBI ranged within 0.67-0.81 with respect to each endpoint. In summary, plasma AFP, kallistatin, and TGFBI may represent valuable non-invasive biomarkers for predicting MIAC or IAI in women with PTL.
Assuntos
Biomarcadores , Proteínas Sanguíneas , Trabalho de Parto Prematuro , Proteômica , Humanos , Feminino , Gravidez , Trabalho de Parto Prematuro/sangue , Adulto , Proteínas Sanguíneas/análise , Proteínas Sanguíneas/metabolismo , Biomarcadores/sangue , Estudos de Casos e Controles , Estudos Retrospectivos , Proteômica/métodos , Corioamnionite/sangue , Corioamnionite/microbiologia , Inflamação/sangue , Amniocentese , Proteoma/análiseRESUMO
PURPOSE: To determine whether various inflammatory-, angiogenic/anti-angiogenic-, and extracellular matrix remodeling-associated proteins in plasma, alone or in combination with conventional blood-based markers, can predict intra-amniotic inflammation and/or microbial invasion of the amniotic cavity (IAI/MIAC) in women with spontaneous preterm labor (PTL). METHODS: A total of 193 singleton pregnant women with PTL (23-33 weeks) were included in this retrospective cohort study. Plasma samples were obtained at the time of amniocentesis. Amniotic fluid (AF) was cultured for microorganism detection and consequent MIAC diagnosis. IL-6 levels were determined in AF and used to identify IAI (AF IL-6 ≥ 2.6 ng/mL). Endostatin, haptoglobin, IGFBP-2/3, LBP, M-CSF, MMP-2/8, pentraxin 3, PlGF, S100A8/A9, and VEGFR-1 levels were assayed in plasma samples by ELISA. CRP levels and neutrophil-to-lymphocyte ratio (NLR) were measured. RESULTS: Plasma LBP, MMP-8, and S100A8/A9 levels, CRP levels, and NLR were significantly higher, and plasma IGFBP-2 and MMP-2 levels were significantly lower in women with IAI/MIAC than in those without this condition, whereas no baseline variables differed significantly between the two groups. Using a stepwise regression analysis, a noninvasive prediction model for IAI/MIAC was developed, which included plasma LBP, MMP-2, and MMP-8 levels (area under the curve [AUC], 0.785). The AUC for this prediction model was significantly or borderline greater than that of any single factor included in the model. CONCLUSIONS: IGFBP-2, LBP, MMP-2, MMP-8, and S100A8/A9 may represent valuable plasma biomarkers for predicting IAI/MIAC in women with PTL. Combination of LBP, MMP-2, and MMP-8 expression data can significantly improve the predictive potential for IAI/MIAC.
Assuntos
Líquido Amniótico , Biomarcadores , Proteína C-Reativa , Corioamnionite , Proteína 2 de Ligação a Fator de Crescimento Semelhante à Insulina , Metaloproteinase 2 da Matriz , Metaloproteinase 8 da Matriz , Trabalho de Parto Prematuro , Humanos , Feminino , Gravidez , Estudos Retrospectivos , Adulto , Trabalho de Parto Prematuro/microbiologia , Trabalho de Parto Prematuro/sangue , Líquido Amniótico/microbiologia , Líquido Amniótico/metabolismo , Metaloproteinase 8 da Matriz/sangue , Proteína C-Reativa/análise , Proteína C-Reativa/metabolismo , Biomarcadores/sangue , Corioamnionite/microbiologia , Corioamnionite/sangue , Proteína 2 de Ligação a Fator de Crescimento Semelhante à Insulina/sangue , Metaloproteinase 2 da Matriz/sangue , Calgranulina A/sangue , Endostatinas/sangue , Proteínas de Fase Aguda/análise , Interleucina-6/sangue , Amniocentese , Componente Amiloide P Sérico/análise , Componente Amiloide P Sérico/metabolismo , Haptoglobinas/análise , Haptoglobinas/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/sangue , Valor Preditivo dos Testes , Matriz Extracelular/metabolismo , Angiogênese , Calgranulina BRESUMO
Abstract: Approximately 10% of individuals do not respond to hepatitis B virus (HBV) vaccination, i.e. non-responders (NRs). We aimed to investigate the association of interleukin (IL)-4 and IL-12B gene polymorphisms with responsiveness to the HBV vaccine in Korean infants. Among 300 healthy infants (9-12 month), SNPs for the IL-4 gene (rs2243250, rs2070874, and rs2227284) and for the IL-12B gene (rs3213094 and rs17860508) were compared between subgroups in terms of the response to HBV vaccination. The percentages of NRs (< 10 mIU/mL), low-titer responders (LRs, 10-100 mIU/mL), and high-titer responders (HRs, ≥ 100 mIU/mL) were 20.3%, 37.7% and 42.0%, respectively. No SNPs differed in frequency between NRs and responders or between LRs and HRs. We divided the subjects into two groups according to the time interval from the 3rd dose of HBV vaccination to Ab quantification: > 6 months from the 3rd dose (n = 87) and ≤ 6 months from the 3rd dose (n = 213). In the ≤ 6 month subjects, rs2243250C and rs2227284G were significantly frequent in the lower-titer individuals (NRs + LR) than HRs (40.1 vs. 25.9%, p = 0.014 and 45.1 vs. 33.0%, p = 0.018, respectively), and the rs2243250C and rs2227284G frequencies were significantly different among the three subgroups (13.2 vs. 26.9 vs. 25.9%, p = 0.040 and 15.5 vs. 29.6 vs. 33.0%, p = 0.038, respectively). In conclusion, those results suggest that IL-4 gene polymorphisms may play a role in the response to the HBV vaccine in Korean infants.
Assuntos
Humanos , Masculino , Feminino , Lactente , Interleucina-4/genética , Vacinas contra Hepatite B/administração & dosagem , Polimorfismo de Nucleotídeo Único , Subunidade p40 da Interleucina-12/genética , Hepatite B/prevenção & controle , Farmacogenética , Fenótipo , Fatores de Tempo , Biomarcadores/sangue , Anticorpos Anti-Hepatite/sangue , Esquemas de Imunização , Vacinação , Resultado do Tratamento , República da Coreia , Frequência do Gene , Hepatite B/genética , Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/sangueRESUMO
ABSTRACT Objective: To investigate the safety and efficacy of allogeneic platelet-rich plasma (PRP) in rotator cuff repair . Methods: Seventeen patients with a full-thickness rotator cuff tear were included. Ten patients underwent arthroscopic rotator cuff repair with allogeneic, and seven patients with autologous PRP. Three PRP gels in a volume 3 ml each were applied between the torn end and the greater tuberosity. Clinical outcomes were assessed preoperatively and at a minimum of 2 years after surgery. Structural outcomes were evaluated with the presence of retear and the change of the cross-sectional area (ACT) of the supraspinatus . Results: Allogeneic PRP did not cause any adverse events during the follow-up period. There was no significant difference in the clinical outcome measures between the two groups (all p > 0.05). The retear rate was 33.3% and 25.0% in the allogeneic group and autologous group, respectively (p = 0.764). The change between the one-year postoperative and immediately postoperative ACT was not also significantly different between the two groups (p = 0.373) . Conclusion: Allogeneic PRP in arthroscopic rotator cuff did not cause any local or general complications and that has the efficacy comparable to autologous PRP with respect to the clinical and structural outcomes. Level of Evidence III, Retrospective Comparative Study.
RESUMO Objetivo: Investigar a segurança e eficácia do plasma rico em plaquetas alogênico (PRP) no reparo do manguito rotador. Métodos: Foram incluídos dezessete pacientes com ruptura da espessura total do manguito rotador. Dez pacientes foram submetidos a reparo artroscópico do manguito rotador com PRP alogênico e sete pacientes com PRP autólogo. Três géis de PRP de 3ml cada foram aplicados entre a extremidade lesionada e a tuberosidade maior. Os resultados clínicos foram avaliados no pré-operatório e no mínimo 2 anos após a cirurgia. Os resultados estruturais foram avaliados com a presença de nova ruptura e a alteração da área em corte transversal (ACT) do supra-espinhal. Resultados: O PRP alogênico não causou quaisquer eventos adversos durante o período de acompanhamento. Não houve diferença significativa nas medidas de resultados clínicos entre os dois grupos (todos os valores p> 0,05). A taxa de nova ruptura foi de 33,3% e 25,0% no grupo alogênico e grupo autólogo, respectivamente (p = 0,764). A alteração da ACT entre o pós-operatório de um ano e imediatamente no pós-operatório também não foi significativamente diferente entre os dois grupos (p = 0,373) . Conclusão: O PRP alogênico administrado por via artroscópica no manguito rotador não causou quaisquer complicações locais ou gerais e sua eficácia é comparável ao PRP autólogo no que diz respeito aos resultados clínicos e estruturais. Nível de Evidência III, Estudo Retrospectivo Comparativo.