A case report of live-stranded rough-toothed dolphin (Steno bredanensis) with plastic debris ingestion on the Pacific coast of Japan.

A juvenile rough-toothed dolphin (Steno bredanensis) was live-stranded and rescued in Kanagawa Prefecture, Japan. From the results of diagnostic examinations, blood tests indicated that the dolphin was malnourished, dehydrated, and anemic. The dolphin died on sixth day of rescue despite treatment. At autopsy, 570 g of foreign material, including 34 pieces of cellophanes and plastic debris (PD), were found in the forestomach. Additional gross findings, including some endoparasitism and presence of accessory spleens were also identified. This is the first case in Japan which accidental ingestion of foreign bodies, including PD, was suspected to be the cause of death in a cetacean.

A first record of digenean parasites of the dwarf sperm whale Kogia sima with morphological and molecular information.

Two species of digenean trematodes of the family Brachycladiidae were obtained from two male dwarf sperm whales Kogia sima that stranded along the island of Kyushu, southern Japan in 2017. From the liver of the first animal, a single, large gravid specimen of a digenean species was collected. The morphological features were consistent with those of the genus Brachycladium. The worm had a large body and was characterized by anterior caeca without lateral diverticula, the shape of testes, ovary, and eggs. Molecular analyses using gene sequences of the 28S rRNA and the mitochondrial NADH dehydrogenase subunit 3 also supported the inclusion of this specimen into the genus Brachycladium. The identity of this worm is undetermined due to the lack of information on the genus and is reported as Brachycladium sp. From the cranial sinuses of the second animal, 33 specimens of digeneans were collected that were morphologically identified as Nasitrema gondo. This report documents a new host record for N. gondo, and the sequence information is provided for this digenean for the first time. This is the second record of digenean parasites for the family Kogiidae, and the first record with morphological and molecular information. The possibility of digenean infection in the liver and cranial sinus should be kept in mind during the necropsy of stranded kogiids.

Molecular phylogeny of the Pseudaliidae (Nematoda) and the origin of associations between lungworms and marine mammals.

Metastrongyloid lungworms from the family Pseudaliidae infect the lungs and cranial sinuses of cetaceans worldwide, except Stenuroides herpestis, which exhibits a striking terrestrial association with the Egyptian mongoose, Herpestes ichneumon. Previous phylogenies of the Metastrongyloidea that included some (2-7) marine species of the Pseudaliidae confirmed that these species are closely related, but also grouped species of Parafilaroides (family Filaroididae) between the members of the Pseudaliidae. In this study we extracted DNA from representatives of all six genera of the Pseudaliidae and amplified the second internal transcribed spacer (ITS2) and cytochrome c oxidase subunit I (cox1) genes in order to investigate the concept of the Pseudaliidae as a monophyletic unit. Three species of Parafilaroides were also included in the analysis. Maximum Likelihood and Bayesian Inference analyses of the concatenated genes resulted in a well-supported clade including the marine pseudaliids, S. herpestis and Parafilaroides spp. These findings validate the status of S. herpestis as a pseudaliid species and support the inclusion of Parafilaroides in the Pseudaliidae. Although males of Parafilaroides spp. lack a copulatory bursa, this trait itself is highly variable in the Pseudaliidae, which include abursate species. Furthermore, life cycles seem to be closely similar between both taxa. When all the available phylogenetic data on the Metastrongyloidea were mapped of onto a phylogeny of the Laurasiatheria, there was a strong suggestion that the Pseudaliidae may have descended from ancestors infecting terrestrial carnivores, with odontocetes being colonized in the marine realm as a result of a host-switching event from pinnipeds through shared fish prey. The origin of the association between S. herpestis and mongooses remains uncertain.

Molecular characterization of the parasitic nematode genus Crassicauda; larvae parasitic on the firefly squid and adults on beaked whales off the coast of Japan.

The Spirurina type X larvae, which infect firefly squid (Watasenia scintillans), are known to cause cutaneous creeping eruption and intestinal obstruction in humans. Although it has been reported that the adult of this larva is Crassicauda giliakiana, which was recovered from a Baird's beaked whale (Berardius bairdii), it is not well known internationally. In this study, to reconfirm the identification of this species, we determined the mitochondrial cox1 gene and the partial sequence of 18S-28S ribosomal DNA from larvae recovered from firefly squid. As the results, we confirmed that the larvae were C. giliakiana, and partial ribosomal DNA sequences were also performed for phylogenetic analysis. Furthermore, to determine the distribution of the genus Crassicauda in Japan as a definitive host, DNA was extracted from archival specimens of adult worms recovered from the kidneys of family Ziphiidae that had stranded on the coast in Japan, and phylogenetic analysis using ITS2 region was conducted. As a result, C. giliakiana were detected from not only B. bairdii but also Mesoplodon stejnegeri, and Ziphius cavirostris, and C. anthonyi was also detected in Z. cavirostris. Furthermore, the kidney parasitic nematoda Crassicauda sp., which is not registered in the database, was found in both M. densirostris and M. gingkodens. This study provides new insights into the distribution and the lifecycle of genus Crassicauda in Japan based on the phylogenetic relationship between larvae and adults.

Disseminated Toxoplasmosis in a Narrow-Ridged Finless Porpoise (Neophocaena asiaeorientalis) with Transplacental Embryonal Transmission.

We describe a case of systemic toxoplasmosis in a female adult narrow-ridged finless porpoise (Neophocaena asiaeorientalis) found in May 2018 inside a gillnet set in the Ariake Sound, southern Japan. The main lesions observed were lymphoplasmacytic and focally necrotizing encephalitis, necrotizing to granulomatous adrenalitis, myocarditis, and inflammation in the intestinal wall, associated with protozoal tissue cysts and tachyzoites. Additionally, the individual had a 5.6 mm (crown-rump length) early-stage embryo in the left uterine horn, which had multifocal necrotizing lesions with intralesional tissue cysts and tachyzoites in the parenchyma. Immunohistochemistry and PCR and sequencing of the internal transcribed spacer 1 region confirmed a Toxoplasma gondii infection. Further genotyping revealed an atypical type II genotype with a type I pattern for the Apico locus. Narrow-ridged finless porpoises are an endangered coastal species already facing various anthropogenic threats. Toxoplasmosis, especially with its ability to transmit to an early-stage embryo, should be considered an emerging threat to this vulnerable species.

Revision of the taxonomic status of Synthesium elongatum (Ozaki, 1935) (Brachycladiidae), an intestinal digenean of narrow-ridged finless porpoise (Neophocaena asiaeorientalis).

Synthesium elongatum (Brachycladiidae) is an intestinal digenean described from the finless porpoise (Neophocaena asiaeorientalis) in Japan. Few records of this species exist and there is a remarkable morphological similarity between S. elongatum and S. tursionis, such that a synonymy between the species has been suggested previously. However, no morphological and/or molecular analysis has been carried out to clarify the taxonomic status of S. elongatum. In this study, we collected specimens of Synthesium sp. from N. asiaeorientalis in western Japan. The specimens possess lobed testes within the third quarter of the body, a round ovary, and vitellaria extending to level of uterine field, which are diagnostic characters for both S. elongatum and S. tursionis. They were morphologically identified to S. elongatum or S. tursionis due to the fact that the available morphometric data for both species overlap remarkably. A molecular analysis of the mitochondrial ND3 gene showed that the pairwise nucleotide distances between these specimens and S. tursionis were small, and phylogenetic analysis showed that these specimens and S. tursionis are in the same clade. Therefore, it was indicated that S. elongatum and S. tursionis are the same species and, consequently, S. elongatum is a synonym of S. tursionis.

Prominent hepatic ductular reaction induced by Oschmarinella macrorchis in a Hubbs' beaked whale Mesoplodon carlhubbsi, with biological notes.

Beaked whales are among the least known group of cetaceans, and information regarding their pathology and parasitology is especially scarce. We describe a case of significant parasitism by a trematode found in the liver of an adult male Hubbs' beaked whale Mesoplodon carlhubbsi that stranded in Hokkaido, Japan. Post-mortem examinations revealed a localised area of discolouration restricted to the hilar region of the left hepatic lobe, where spindle-shaped trematodes occupied the dilated and hypertrophic bile ducts. Histologically, the intrahepatic bile ducts were characterised by adenomatous hyperplasia with goblet cell metaplasia of the biliary epithelium. Findings in the adjacent hepatic parenchyma included pseudocarcinomatous ductular reactions obliterating hepatocytes, a histomorphology not previously reported in marine mammals. Morphological identification of the trematode corresponded to Oschmarinella macrorchis, which has only been reported once in a Stejneger's beaked whale, M. stejnegeri. PCR amplification and sequencing analyses of the parasite's mtDNA ND3, 18S and 28S rRNA regions generated novel gene sequences. Environmental contaminant levels were measured to explore its potential relationship with the parasitism but there was no conclusive association. A high level of polychlorinated biphenyl (30000 ng g-1 lipid weight) was detected in the blubber of this individual, when compared to those of 3 other male Hubbs' beaked whales stranded in Japan. Stomach contents were also analysed, indicating the presence of various squid species and unidentified fish. Our results contribute to the knowledge of a little-known beaked whale and provide evidence for the first time of the pathobiological response caused by O. macrorchis.

Population- and growth-related differences in helminthic fauna of finless porpoises (Neophocaena asiaeorientalis) in five Japanese populations.

A survey of helminths and ectoparasites, including epizoits, was conducted in narrow-ridged finless porpoises (Neophocaena asiaeorientalis) from Japanese five populations using dead stranded or incidentally caught animals. In total, 13 helminth species were found (6 nematodes, 4 trematodes, 2 cestodes and 1 acanthocephalan) in 137 porpoises. A new location record of Stenurus nanjingensis and a new host record of Tetrabothrius sp. were obtained. Eight species of helminth were considered common in the Japanese populations of the finless porpoise: Pharurus sunameri, Pharurus asiaeorientalis, Nasitrema spathulatum, Nasitrema sunameri, Halocercus pingi, Halocercus sunameri, Campula oblonga and Synthesium elongatum. No anisakid nematodes were found. N. spathulatum was found only in the western waters of the Seto Inland Sea. Low prevalence of C. oblonga in the Omura Bay was demonstrated. H. pingi was mostly found in very young porpoises before starting to eat prey, indicating prenatal or transmammary infection. However, a congeneric species, H. sunameri, mainly infected weaned porpoises, indicating that these two species possess different transmission pathways. This study provides information on the geographical distribution and prevalence of helminth parasites in finless porpoises off the Japanese coast.

Polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated analogues in the blood of harbor, Dall's and finless porpoises from the Japanese coastal waters.

This study investigated the accumulation of polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated analogues (OH-PBDEs and MeO-PBDEs) in the blood of harbor porpoises, Dall's porpoises, and finless porpoises stranded or bycaught in Japanese coastal waters and in the North Pacific Ocean. Moreover, we suggested the origins of these contaminants and the factors affecting their pattern of accumulation. Levels of PBDEs in Dall's porpoises were one order of magnitude greater than those in the other species. OH-PBDE and MeO-PBDE levels were comparable to those of PBDEs. However, no correlation was found between the levels of OH-PBDEs and PBDEs, whereas a strong correlation was found between that of OH-PBDEs and MeO-PBDEs (p < 0.001). 6OH-BDE47, reported compound biosynthesized by marine low-trophic level organisms, was the dominant congener. These results suggest that PBDEs found in these porpoise species derive from flame retardants, but OH-PBDEs and MeO-PBDEs are mainly of natural origins.

Locking versus non-locking neutralization plates for treatment of lateral malleolar fractures: a randomized controlled trial.

PURPOSE: The reduction of periosteal compression through the use of a locking plate may minimize disturbances of bone blood supply and may improve the rate of bone union. A single-centre, assessor blinded randomized controlled trial was conducted to compare the clinical effectiveness of a locking plate and a non-locking plate. METHODS: A total of 52 patients with AO/OTA 44B lateral malleolar fractures were included in this study. All patients underwent surgical fixation using a lag screw and neutralization plate. An identical treatment protocol was used in all patients, with exception of plate selection. The rate of radiographic bone union, defined as the complete disappearance of fracture lines confirmed through anteroposterior, lateral, and internal oblique views was compared at three, six, and 12 months following surgery. In addition, the Medical Outcomes 36-Item Short-Form Health Survey (SF-36) score, the time required for resolution of tenderness at the fracture site and the complication rate were evaluated. RESULTS: Twenty-three patients were randomly assigned to undergo fixation using a locking plate, and 29 patients were assigned to undergo fixation using a non-locking plate. Intention-to-treat analysis showed no difference in the radiographic bone union rate of fibula, SF-36 score, the time for resolution of tenderness at the fracture site and complication rates. CONCLUSION: No differences were observed in patients with AO/OTA 44B lateral malleolar fractures undergoing fixation with a locking versus non-locking neutralization plate.

A study on visualizing the twist and the load on the lumbar region in nursing care.

Most nurses suffer from lower back pain (LBP). The load on the lumbar region in nursing practice depends on patient's condition and situation. So we regard it as the load of nursing practice, and suggest visualizing the load on the lumbar region for nursing management. This study focuses on "twist" which is one of factors of LBP. We measured the twist angle of the lumbar region and the surface electromyogram (EMG) in three methods of transferring a patient from a bed to a wheelchair. Experimental subjects were seven women in their 20s and 30s who had learned the transferring technique. Measurement was performed using TRIAS system software (DKH Co., Ltd., Japan). The obtained signal data were analyzed using software (Matlab; The MathWorks, Inc.). We compared three methods, each of which was divided into four motions according to the experimental purpose. Results of visualizing the differences of the load on the lumbar region by twist angle, twist angular velocity and twist angular acceleration in each method could indicate nursing practice of the less load.

Investigation of the load on the lumbar region in nursing technique's movements - relation between twist and surface electromyogram.

This study measured the twist angle of the lumbar region and the surface electromyogram (EMG) and examined their mutual relation to elucidate the degree and influence of factors of "twist" in nursing techniques as a cause of lower back pain. Using a goniometer (two-way angle and twist sensors) and an EMG(SX230; DKH Co., Ltd.), we conducted measurements by affixing the goniometer on the lumbar vertebral column and EMG sensor at four points of right and left sides of L2 and L4 (of the erector muscle of the spine). The measured nursing techniques were three common methods of "transferring a patient from bed to wheelchair," which is said to impart a heavy load on the lumbar region. Results show that the correlation value between the twist angle rate and mean energy is likely to be greater, suggesting that the magnitude of the load on the lumbar region should be related to the twist speed rather than to the twist angle of the movement itself.

Evaluation of the accuracy of CT numbers in statistical correction of nonlinearity for polychromatic X-ray CT projection data.

The CT number is theoretically independent of the number of incident photons and the thickness of a subject. However, when noise is added, the CT number becomes dependent on the number of incident photons because of the nonlinearity of the logarithm operation, and so the accuracy of the CT number is degraded, especially in a photon-starved state. The inconsistency of the CT number due to nonlinearity generates an intense streak artifact in a reconstructed image. We have theoretically clarified the statistical characteristics of both the nonlinearity of the logarithm transform and the non-zero/non-negative restriction in the logarithm operation. Moreover, we have formulated a correction method for such nonlinearity according to the statistical distribution of noise, and we carried out computer simulations for monochromatic X-ray beams. However, the applicability to polychromatic-energy photons has not been confirmed. The artifacts induced by the beam-hardening effect, which occurs when incident beams are polychromatic, remarkably deteriorate the accuracy of the CT number. In this paper, we quantitatively evaluate the improvement of the quality of reconstructed images by our correction method by using a numerical experiment when incident beams are polychromatic. The experimental results show that there is less influence on spatial resolution and that the CT number is hardly dependent on the number of incident photons for a polychromatic beam as well as a monochromatic beam. Moreover, the results suggest the possibility that the same image quality is obtained at a much lower exposure level than the clinical exposure level of low-dose CT.

Differentiation of myocardial infarction and angina pectoris by processing ultrasonic color kinesis images.

PURPOSE: The aim of this study was to develop a method for early, accurate differentiation between old myocardial infarction (OMI) and angina pectoris (AP) using color kinesis (CK) images. We first extracted exact end-diastolic and end-systolic contours from CK images and then extracted the features of cardiac function from two CK images (one at rest, the other after exercise) and investigated their effectiveness in differentiating old myocardial infarction and angina pectoris. We then evaluated the effectiveness of several features in recognizing coronary artery disease and used the effective features to show the differentiation results. METHODS: First, we extracted exact end-diastolic and end-systolic contours from CK images with an active contour model. Second, we defined the features that seemed to be effective in recognizing coronary artery disease. The features are extracted from the region between the end-diastolic endocardial contour and end-systolic endocardial contour in two CK images: one obtained when the subject was at rest and the other after exercise. Nine features were considered effective for differentiating old myocardial infarction and angina pectoris, and the effectiveness in recognizing coronary artery disease, which includes old myocardial infarction and angina pectoris, was evaluated. Third, coronary artery disease is recognized by the effective features. RESULTS: Contours near a manual trace by a skilled physician were obtained using the proposed method. Multiple comparisons of the mean values of the extracted features were drawn among three groups: a healthy-subject group; an old myocardial infarction patient group; and an angina pectoris patient group. The feature effective in differentiating old myocardial infarction was the "area at rest"; those effective in differentiating angina pectoris were a "decrease in area" and a "decrease in movement." These effective features have almost always differentiated old myocardial infarction and angina pectoris. CONCLUSIONS: This study used the endocardial contour extraction technique with the dynamic contour model and evaluated the validity of the features of cardiac function; it then recognized coronary artery disease from the effective features. Multiple comparisons of the mean value of the extracted features among the healthy-subject group, the old myocardial infarction patient group, and the angina pectoris patient group has proved that the "area at rest" is effective in differentiating old myocardial infarction, and the "decrease in area" and "decrease in movement" are effective for differentiating angina pectoris.