1.
MicroPubl Biol
; 20232023.
Artigo
em Inglês
| MEDLINE
| ID: mdl-36824382
RESUMO
CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast.