Development of a "turn off" fluorescent molecularly imprinted nanoparticle-based sensor for selective captopril quantification in synthetic urine and wastewater samples.

The combination of silica nanoparticles with fluorescent molecularly imprinted polymers (Si-FMIPs) prepared by a one-pot sol-gel synthesis method to act as chemical sensors for the selective and sensitive determination of captopril is described. Several analytical parameters were optimized, including reagent ratio, solvent, concentration of Si-FMIP solutions, and contact time. Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and the ninhydrin assay were used for characterization. The selectivity was evaluated against molecules belonging to other drug classes, such as fluoroquinolones, nonacid nonopioids, benzothiadiazine, alpha amino acids, and nitroimidazoles. Under optimized conditions, the Si-FMIP-based sensor exhibited a working range of 1-15 µM, with a limit of detection (LOD) of 0.7 µM, repeatability of 6.4% (n = 10), and suitable recovery values at three concentration levels (98.5% (1.5 µM), 99.9% (3.5 µM), and 99.2% (7.5 µM)) for wastewater samples. The sensor provided a working range of 0.5-15 µM for synthetic urine samples, with an LOD of 0.4 µM and a repeatability of 7.4% (n = 10) and recovery values of 93.7%, 92.9%, and 98.0% for 1.0 µM, 3.5 µM, and 10 µM, respectively. In conclusion, our single-vessel synthesis approach for Si-FMIPs proved to be highly effective for the selective determination of captopril in wastewater and synthetic urine samples.

Monitoring Ozone Using Portable Substrate-Integrated Hollow Waveguide-Based Absorbance Sensors in the Ultraviolet Range.

Ozone is an oxidizing molecule used for disinfecting a wide variety of environments, such as in dental clinics, and has most recently been promoted as a sanitizing agent to prevent coronavirus transmission. The easy access to ozone-generating sources also enables their ubiquitous use. However, exposure to ozone may seriously affect human health by amplifying or inducing respiratory diseases and distress syndromes and has been associated with premature deaths from other diseases. In this scenario, miniaturized, low-cost, and portable optical sensors based on the absorption signature of ozone in the ultraviolet (UV) range of the electromagnetic spectrum are an innovative approach for providing real-time monitoring of gaseous ozone, ensuring the safety of indoor and workplace environments. In this paper, a miniaturized ozone sensor based on the absorption signature of ozone at deep-UV frequencies was developed by integration of so-called substrate-integrated hollow waveguides (iHWG) with a miniaturized ultraviolet lamp and a fiber-optic USB-connected spectrophotometer. The innovative concept of iHWGs facilitates unprecedented compact dimensions with a high degree of flexibility in the optical design of the actual photon absorption path. The proposed device rapidly responded to the presence of ozone (<1 min) and revealed a suitable linearity (r 2 > 0.99) in the evaluated concentration range. The limit of detection was determined at 29.4 ppbv, which renders the device suitable for measurements in the threshold range of the main regulatory agencies. Given the adaptability and modularity of this platform, we anticipate the application of this innovative concept to be equally suitable for the in situ and real-time analysis of other relevant gases providing suitable UV absorption signatures.