Combined Effect of Low-Intensity Helium-Neon Laser and X-Ray Radiation on in Vivo Cellular Response of the Whole Blood and Lymphoid Organs in Mice.

We studied the effect of exposure to helium-neon laser (dose range 0.16-50 mJ/cm2) on activation of natural protection reserve in mice using the adaptive response test. DNA comets method revealed a protective response manifested in DNA damage level in whole blood leukocytes of mice and in lymphoid organs by the thymus and spleen weight index; preexposure to laser did not induce the adaptive response. ROS level in the whole blood was assessed by the level of zymosan-induced luminol chemiluminescence. In mice subjected to adaptive laser irradiation in doses of 0.16-5 mJ/cm2 followed by X-ray irradiation in a dose of 1.5 Gy, the activation index calculated as the ratio of induced to spontaneous area of luminescence was by 1.4 times lower than that in non-irradiated animals, which attested to reduced ROSgeneration reserve capacity of neutrophils.

Induction of the Adaptive Response in Mice Exposed to He-Ne Laser and X-Ray Radiation.

We studied the dose-dependent induction of in vivo adaptive response in the bone marrow and blood of mice exposed to low-intensity radiation of He-Ne laser (633 nm) and X-ray radiation by the severity of cytogenetic injury and intensity of ROS production, respectively. Induction of the adaptive response in mice preexposed to He-Ne laser and X-ray radiation depended on the adaptive dose and the interval between the adaptive and main doses and correlated with changes in ROS generation. The adaptive response after exposure to low-intensity ionizing and non-ionizing radiation was observed in the same dose range, which attests to similar mechanisms of its induction.

Effect of sunlight transformed by luminophore-containing materials on cell functions in vitro and in vivo.

Effect of sunlight transformed by luminophore-containing materials on cell viability and functional state of the retina was assessed using the photodamage model. Exposure to the luminescent component of light improved viability of NIH 3T3 cells and promoted recovery of electric activity in rabbit retina after photodamage.

[Influence of low-dose-rate red and near-infrared radiations on the level of reactive oxygen species, the genetic apparatus and the tumor growth in mice in vivo].

The effect of low-dose-rate red and near-infrared radiations from the matrix of light emitted diode (650 nm and 850 nm) and a He-Ne laser (633 nm) on activation of the reserve of a natural defense system in the mice exposed to radiation in vivo was studied by the level of reactive oxygen species (ROS) production in blood cells, the induction of cytogenetic adaptive response in bone marrow cells, thymus and spleen, and the rate of Ehrlich ascites carcinoma growth in a solid form. As a positive control animals were irradiated with X-rays by the scheme of the radiation-induced adaptive response (0.1 Gy + 1.5 Gy). The levels of ROS production was assessed in whole blood by luminol-dependent chemiluminescence, of cytogenetic damage--by the "micronucleus test" in the bone marrow, the weight of the thymus and spleen--by index of organ, and the rate of tumor growth--according to its size for 30 days after inoculation. Adaptogenic and anticarcinogenic effects of studied radiations were revealed. The values of these effects were not different from those in animals pre-irradiated with the X-rays. The relationship between the level of ROS production and adaptive response induction in the mice under the influence of non-ionizing radiation was first ascertained. The experimental data obtained may indicate a similar mechanism of induction of protective responses to ionizing and non-ionizing radiations in mice in vivo.

[The strategy of the "useful sun" improves physical endurance and structural adaptation in the myocardium].

The action of solar light transformed by special screens has been studied on CD-1 male mice. In the active control group, mice were irradiated through screens absorbing the UV-component. In the experimental group, screens transforming the UV-component into the orange-red light were used. In the active control, changes in the swimming activity, as compared to the same parameter before irradiation, were manifested much less than in animals of the experimental group. A morphological analysis showed changes in the structure of all cardiomyocyte organelles studied: the relative area of mitochondria in the experimental mice increased by more than 20% compared to intact animals (p < 0.05). A significant increase in the area of the sarcoplasmic reticulum, by 23.4% (p < 0.05), and in the volume of the myofibrillar apparatus, by 19.4% (p < 0.05), was detected. The results of our experiment show that the irradiation with using an additional orange-red component improves the physical endurance 1.5 times and initiates morphogenetic processes in cardiac muscle cells.

[Orange-red light reduces the inhibition by UV-A of the proliferation of rat fibroblasts and influences their attachment in a dose-dependent fashion].

The effects of ultraviolet-A (UV-A, lambdam = 365 nm) and orange-red light (lambdam = 625 nm) on the attachment and proliferation of embryonic skin-muscle rat fibroblasts were investigated. It was found that orange-red light (ORL) produces both the stimulatory and inhibitory actions on the attachment and proliferation of fibroblasts, whereas UV-A radiation caused only the inhibition of the processes. Upon consecutive irradiation in both variants: ORL --> UV and UV --> ORL, the synergistic effect of the inhibitory action in both spectral regions was observed. Conversely, upon simultaneous irradiation with ORL (dose 3.6 J/cm2) and UV-A (dose 1.8 J/cm2), the inhibitory effect of ORL and UV-A weakened. Possible mechanisms of the effects are analyzed.

[Laboratory diagnosis of changes in regulation of the immune system]. / Laboratornaia diagnostika izmeneniia reguliatsii immunnoi systemy.

To evaluate the body's immunity, to diagnose immunodeficiencies is a pressing problem. The paper discusses whether a complex approach can be used to evaluate immunity objectively. The data on humoral and cellular immunity in patients with varying responses to the changes occurring in the body are analyzed. The changes in cellular and humoral immunity were studied by enzyme immunoassay of hormones and antibodies in combination with immunomorphological assay of lymphocytic subpopulations.

Extraction and stability of the pigment-protein complexes of the photosystem 2 from membranes of the unicellular alga Dunaliella salina: effect of glycerol.

Effects of glycerol on the extraction efficiency and the stability of pigment-protein complexes (PPC) of photosystem 2 (PS2) from membranes of the green unicellular alga Dunaliella salina were studied. PS2 membranes were extracted with the detergent n-dodecyl-beta-maltoside (DM) in the presence of 4 M glycerol and 1 M NaCl at a DM:chlorophyll ratio of 15:1 (w/w). The extracts were purified on a DEAE fractogel 650S column. To assess the effect of glycerol on the PPC stability, PS2 membranes were illuminated with white light of high intensity. Then PPCs were extracted and studied spectroscopically and using PAG electrophoresis under denaturing and native conditions. It was found that addition of 4 M glycerol and 1 M NaCl to the extraction medium affects the extraction of the PS2 PPCs but not of the light-harvesting chlorophyll a/b protein (LH2). It was shown that 4 M glycerol protects PS2 from the destructive effects of intense white light, high NaCl concentration, and the detergent Triton X-100. It is suggested that, apart from its osmotic action, glycerol performs a protective function and stabilizes PS2 PPCs in the cells of Dunaliella salina.