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BACKGROUND: In the fields of medico-legal matters and bio-archaeological settings, gender evaluation plays a pivotal role in the initial stages of human identification. Approximately half of the population at risk is excluded when gender is determined, making it the most essential factor for identification. When it comes to medico-legal matters and bio-archaeological settings, gender evaluation is a crucial initial step in human identification. Traditional gender determination procedures, such as skull and pelvic analysis, may be hindered by fragmentary human remains that have been degraded by various forms of inhumation or physical assaults. AIM: To investigate sexual dimorphism, this study examined the ratio of index finger length to ring finger length. MATERIALS AND METHOD: The lengths of the index and ring fingers were measured and the ratios between them were calculated for both hands separately. Applications of IBM SPSS Statistics for Windows, Version 16.0 (Released 2007; IBM Corp., Armonk, New York, United States) included Student's t-test and Levene's test. RESULTS: According to the study, women's index finger-to-ring finger length ratios were much longer than men's. The ratio of index finger to ring finger length was significantly different between the sexes on both sides of the hand (p<0.001). In terms of the right hand, the threshold value was 0.9666 for men and 0.9952 for females, while in terms of the left hand, the values were 0.9638 and 0.9920, respectively. CONCLUSION: With an advancing front in this arena on gender determination, the use of digits has become an additional source of support to physical anthropologists for bio-archaeological surveys and to forensic experts for use in medico-legal investigations for fragmentary remains received during investigatory trials.
RESUMO
Objectives: Picrosirius red and MMP are capable of degrading extracellular matrix proteins, expressed in lesions such as squamous cell carcinomas. The present study was undertaken with an aim to analyze and compare changes in collagen using Picrosirius red staining under polarizing microscopy and immunohistochemical staining using anti MMP-13 in samples of oral leukoplakia, oral submucous fibrosis and oral squamous cell carcinoma. Materials and Methods: A total of 70 slides were prepared and divided into 3 groups. Group I comprised 10 slides of normal gingival tissue, Group II 40 slides of potentially malignant disorders and Group III 20 slides of well differentiated oral squamous cell carcinoma. Half the slides for each group were stained with Picrosirius red stain and the remainder with antibodies to MMP-13. Rerults: In Group II, MMP-13 connective tissue expression was greater in OSMF as compared to leukoplakia. Group III showed elevated expression among 70% of cases. Picrosirius red staining in Group II cases, showed higher staining Yellow-Orange andGreen-Yellow mature fibers in OSMF than leukoplakia cases while in Group III, 50% OSCC cases showed Green-yellow stained immature thin fibers. Conclusion: In future, therapeutic measures targeted against MMP-13 may inhibit collagenolysis to some extent and delay spread of tumors. An easy and reliable method to determine the state of the stroma in such cases may be Picrosirius red staining with polarizing microscopy.