Exploring the therapeutic potential of allogeneic amniotic membrane for quality wound healing in rabbit model.

BACKGROUND: The amniotic membrane (AM) has shown immense potential in repairing wounds due to its great regenerative qualities. Although the role of AM as a biological scaffold in repairing wounds has been studied well, the tissue regenerative potential of AM-derived mesenchymal stem cells (MSCs) and conditioned media (CM) derived from it remains to be discovered as of now. Here, we examined the wound healing abilities of fresh and frozen thawed rabbit AM (rAM) along with the MSCs and their lyophilised CM in rabbits challenged with skin wounds. METHODS: To elucidate the role of rAM-MSCs and its CM in repairing the wound, we isolated it from the freshly derived placenta and characterised their differentiation potential by performing an in vitro tri-lineage differentiation assay besides other standard confirmations. We compared the wound repair capacities of rAM-MSCs and lyophilised CM with the fresh and cryopreserved AM at different timelines by applying them to excision wounds created in rabbits. RESULTS: By monitoring wound contractions and tissue histology of wounded skin at different time points after the application, we observed that rAM-MSCs and rAM-MSC-derived CM significantly promoted wound closure compared to the control group. We also observed that the wound closure capacity of rAM-MSCs and rAM-MSC-derived CM is as efficient as fresh and cryopreserved rAM. CONCLUSION: Our findings suggest that rAM-MSCs and rAM-MSC derived CM can be effectively used to treat skin wounds in animals and correctly delivered to the damaged tissue using AM as a bioscaffold, either fresh or frozen.

Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep.

Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7th days postinoculation (DPI) and in thyroid and pituitary glands at 15th DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.

Experimental and Theoretical Investigations of MAPbX3 -Based Perovskites (X=Cl, Br, I) for Photovoltaic Applications.

This work mainly focuses on synthesizing and evaluating the efficiency of methylammonium lead halide-based perovskite (MAPbX3 ; X=Cl, Br, I) solar cells. We used the colloidal Hot-injection method (HIM) to synthesize MAPbX3 (X=Cl, Br, I) perovskites using the specific precursors and organic solvents under ambient conditions. We studied the structural, morphological and optical properties of MAPbX3 perovskites using XRD, FESEM, TEM, UV-Vis, PL and TRPL (time-resolved photoluminescence) characterization techniques. The particle size and morphology of these perovskites vary with respect to the halide variation. The MAPbI3 perovskite possesses a low band gap and low carrier lifetime but delivers the highest PCE among other halide perovskite samples, making it a promising candidate for solar cell technology. To further enrich the investigations, the conversion efficiency of the MAPbX3 perovskites has been evaluated through extensive device simulations. Here, the optical constants, band gap energy and carrier lifetime of MAPbX3 were used for simulating three different perovskite solar cells, namely I, Cl or Br halide-based perovskite solar cells. MAPbI3 , MAPbBr3 and MAPbCl3 absorber layer-based devices showed ~13.7 %, 6.9 % and 5.0 % conversion efficiency. The correlation between the experimental and SCAPS simulation data for HIM-synthesized MAPBX3 -based perovskites has been reported for the first time.

SnO2-Based NO2 Gas Sensor with Outstanding Sensing Performance at Room Temperature.

The controlled and efficient formation of oxygen vacancies on the surface of metal oxide semiconductors is required for their use in gas sensors. This work addresses the gas-sensing behaviour of tin oxide (SnO2) nanoparticles for nitrogen oxide (NO2), NH3, CO, and H2S detection at various temperatures. Synthesis of SnO2 powder and deposition of SnO2 film is conducted using sol-gel and spin-coating methods, respectively, as these methods are cost-effective and easy to handle. The structural, morphological, and optoelectrical properties of nanocrystalline SnO2 films were studied using XRD, SEM, and UV-visible characterizations. The gas sensitivity of the film was tested by a two-probe resistivity measurement device, showing a better response for the NO2 and outstanding low-concentration detection capacity (down to 0.5 ppm). The anomalous relationship between specific surface area and gas-sensing performance indicates the SnO2 surface's higher oxygen vacancies. The sensor depicts a high sensitivity at 2 ppm for NO2 with response and recovery times of 184 s and 432 s, respectively, at room temperature. The result demonstrates that oxygen vacancies can significantly improve the gas-sensing capability of metal oxide semiconductors.

Highly Responsive Near-Infrared Si/Sb2Se3 Photodetector via Surface Engineering of Silicon.

The development of imaging technology and optical communication demands a photodetector with high responsiveness. As demonstrated by microfabrication and nanofabrication technology advancements, recent progress in plasmonic sensor technologies can address this need. However, these photodetectors have low optical absorption and ineffective charge carrier transport efficiency. Sb2Se3 is light-sensitive material with a high absorption coefficient, making it suitable for photodetector applications. We developed an efficient, scalable, low-cost near-infrared (NIR) photodetector based on a nanostructured Sb2Se3 film deposited on p-type micropyramidal Si (made via the wet chemical etching process), working on photoconductive phenomena. Our results proved that, at the optimized thickness of the Sb2Se3 layer, the proposed Si micropyramidal substrate enhanced the responsivity nearly two times, compared with that of the Sb2Se3 deposited on a flat Si reference sample and a glass/Sb2Se3 sample at 1064 nm (power density = 15 mW/cm2). More interestingly, the micropyramidal silicon-based device worked at 0 V bias, paving a path for self-bias devices. The highest specific detectivity of 2.25 × 1015 Jones was achieved at 15 mW/cm2 power density at a bias voltage of 0.5 V. It is demonstrated that the enhanced responsivity was closely linked with field enhancement due to the Kretschmann configuration of Si pyramids, which acts as hot spots for Si/Sb2Se3 junction. A high responsivity of 47.8 A W-1 proved it suitable for scalable and cost-effective plasmonic-based NIR photodetectors.

Draft Genome Sequence of a Pasteurella multocida Strain Isolated from a Spotted Deer (Axis axis) in India.

Here, we report the genome sequence of a Pasteurella multocida strain isolated from the heart blood of a spotted deer (Bareilly, India). The 2.44-Mbp genome has 2,227 coding sequences, with a G+C content of 40.7%.

Biochanin-A alleviates fibrosis and inflammation in cardiac injury in mice.

Biochanin-A (BCA), is an isoflavonoid, exhibits protective effects against various diseases. This study was conducted to observe the effect of BCA on isoprenaline (ISP)-induced cardiac fibrosis and explore the underlying mechanism. The curative effect of BCA was investigated with oral administration for 14 days in ISP-induced cardiac fibrosis in mice. The fibrotic biomarkers, like collagen I and III, were estimated by ELISA. Commercial kits were used to estimate cholesterol, triglycerides, and creatine kinase-myocardial band (CK-MB) levels. The messenger ribonucleic acid (mRNA) expression studies were performed by quantitative real-time polymerase chain reaction. Gelatin zymography was used to study the expression of matrix metalloproteinases-2 (MMP-2). BCA co-administration significantly improved the morphometric parameters; including heart weight, heart weight to body weight, heart weight to tibial length, and lipid profile. BCA treatment showed a reduction in inflammatory cells and collagen deposition as depicted in the histopathology of heart tissues. The enhanced levels of collagen-I, III, and hydroxyproline were significantly decreased by BCA co-treatment, whereas CK-MB level was reduced slightly. BCA co-administration increased the activity of reduced glutathione enzyme, showing the antioxidative effects of BCA. BCA treatment significantly reduced interleukin-6 (Il6) inflammatory cytokine along with partially decreased mRNA expression of fibrotic signaling markers such as natriuretic peptide type B (Nppb), α-smooth muscle actin (Acta2), connective tissue growth factor (Ctgf), transforming growth factor ß (Tgfb), small mothers against decapentaplegic homolog-3 (Smad-3). However, BCA did not modify Mmp-2 expression, which was significantly increased by ISP. In conclusion, BCA exerts an antifibrotic effect by modulating lipid profile, enhancing antioxidant enzyme, and reducing collagen content and inflammation.

Thermally Deposited Sb2Se3/CdS-Based Solar Cell: Experimental and Theoretical Analysis.

As a promising solar absorber material, antimony selenide (Sb2Se3) has gained popularity. However, a lack of knowledge regarding material and device physics has slowed the rapid growth of Sb2Se3-based devices. This study compares the experimental and computational analysis of the photovoltaic performance of Sb2Se3-/CdS-based solar cells. We construct a specific device that may be produced in any lab using the thermal evaporation technique. Experimentally, efficiency is improved from 0.96% to 1.36% by varying the absorber's thickness. Experimental information on Sb2Se3, such as the band gap and thickness, is used in the simulation to check the performance of the device after the optimization of various other parameters, including the series and shunt resistance, and a theoretical maximum efficiency of 4.42% is achieved. Further, the device's efficiency is improved to 11.27% by optimizing the various parameters of the active layer. It thus is demonstrated that the band gap and thickness of active layers strongly affect the overall performance of a photovoltaic device.

24% Efficient, Simple ZnSe/Sb2Se3 Heterojunction Solar Cell: An Analysis of PV Characteristics and Defects.

In this work, a new wide-band-gap n-type buffer layer, ZnSe, has been proposed and investigated for an antimony selenide (Sb2Se3)-based thin-film solar cell. The study aims to boost the Sb2Se3-based solar cell's performance by incorporating a cheap, widely accessible ZnSe buffer layer into the solar cell structure as a replacement for the CdS layer. Solar Cell Capacitance Simulator in One Dimension (SCAPS-1D) simulation software is used to thoroughly analyze the photovoltaic parameters of the heterojunction structure ZnSe/Sb2Se3. It includes open circuit voltage (V OC), short-circuit current density (J SC), fill factor (FF), power conversion efficiency (PCE), and external quantum efficiency (EQE). The absorber layer (Sb2Se3) thickness is adjusted from 0.5 to 3.0 µm to perfect the device. In addition, the influence of cell resistances, radiative recombination coefficient, acceptor and donor defect concentration in the Sb2Se3 layer, and interface defects of the ZnSe/Sb2Se3 layer on overall device performance are investigated. The ZnSe buffer layer and the Sb2Se3 absorber layer are designed to have optimal thicknesses of 100 nm and 1.5 µm, respectively. The proposed device's efficiency with optimized parameters is calculated to be 24%. According to the simulation results, it is possible to build Sb2Se3-based thin-film solar devices at a low cost and with high efficiency by incorporating ZnSe as an electron transport layer.

Na ion batteries: An India centric review.

Developing low-cost and safe energy storage devices is the primary goal of every country to make a carbon-neutral atmosphere by ∼2050. Batteries and supercapacitors are the backbones of future sustainable energy sources for electrical vehicles (EVs), smart electronic devices, electricity supply to off-grid regions, etc. Hence, these battery-dependent devices are substantially gaining the market. Although lithium-ion batteries account for powering most of these devices, lithium availability and price pose a severe problem since lithium resources are not abundant in nature. Thus, alternative research on sodium-ion or other multi-charged cations (Al3+/Mg2+/Ca2+/K+) based energy storage devices is needed to substitute lithium-ion batteries. India and many other countries have sodium in abundance. Sodium also has potential in designing and developing efficient charge storage devices. This review article discusses the status of sodium-ion battery research activities, cost, market analysis, and future strategies of the Indian government or private bodies, industries, and research institutes of India.

Temperature-Dependent n-p-n Switching and Highly Selective Room-Temperature n-SnSe2/p-SnO/n-SnSe Heterojunction-Based NO2 Gas Sensor.

Many toxic gases are mixed into the atmosphere because of increased air pollution. An efficient gas sensor is required to detect these poisonous gases with its ultrasensitive ability. We employed the thermal evaporation method to deposit an n-SnSe2/p-SnO/n-SnSe heterojunction and observed a temperature-dependent n-p-n switching NO2 gas sensor with high selectivity working at room temperature (RT). The structural and morphological properties of the material were studied using the characterization techniques such as XRD, SEM, Raman spectroscopy, XPS, and HRTEM, respectively. At RT, the device response was 256% for 5 ppm NO2. The response/recovery times were 34 s/272 s, respectively. The calculated limit of detection (LOD) was ∼115 ppb with a 38% response. The device response was better with NO2 gas than with SO2, NO, H2S, CO, H2, and NH3. The mechanism of temperature-dependent n-p-n switching, fast response, recovery, and selective detection of NO2 at RT has been discussed on the basis of physisorption and charge transfer. Thus, this work will add a new dimension to 2D materials as selective gas detectors at room temperature.

Detection and immune cell response of natural maedi visna virus (MVV) infection in Indian sheep and goats.

Maedi is a lentiviral disease characterized by progressive interstitial pneumonia with humoural as well as cell mediated immune response. The present investigation was designed to detect the presence of MVV in different biological samples and to evaluate the immune response in naturally MVV infected sheep and goats. Total of 701 biological samples (289 lung tissues, 233 blood, 54 brain tissues, 74 mammary gland tissues and 51 joint tissues were screened for the MVV by nested PCR. MVV nucleic acid was detected in 10.41% of samples and it was observed that sheep samples showed positivity of 8.7% and goat samples 12.6%. Blood samples showed highest positivity (14.59%) followed by joint tissue (13.72), lungs (8.6%), mammary gland (8.1%) and brain (1.85%). MVV p28 antigen was detected in the cytoplasm of mononuclear cells, particularly in the macrophages of lungs and lymph nodes. Antibodies against SRLVs were detected by cELISA and seroprevalence of 19.58% was observed in both sheep and goats serum samples. The seropositivity was higher in sheep (22.9%) as compared to the goats (15.59%). IHC was done to identify the nature of the immune cells infiltrated in the MVV infected tissues and it was observed that B cells, CD8+ and macrophages were the predominant immune cells infiltrated in the lungs showing MVV infection. Expression of the cytokines was assessed by real time PCR and it was observed that expression of IL-10, IFN-γ, TNFα, IL-4, IL-2 and IL6 was down regulated in most of the cases but few samples showed upregulation. In conclusion, MVV is circulating in the sheep and goat population of the India and the disease causes altered immune response in the animal which may make the infected animals more prone to other infectious diseases.

Molecular epidemiological studies on avian pathogenic Escherichia coli associated with septicemia in chickens in India.

Bacterial septicemia causes huge economic losses in the poultry industry and there is no systematic research available in India on the connection of various pathogens associated with septicemia. The present molecular epidemiological study was conducted to investigate the association of different bacterial and immunosuppressive viral pathogens in septicemia suspected chickens. A total of 443 chicken carcasses with septicemic conditions from 71 different flocks were included in this study. Heart blood swabs were subjected to bacterial culture for Salmonella spp., Pasteurella multocida, Escherichia coli, and Gallibacterium anatis. Of these 51 flocks tested for E. coli, 49 (96.1%) flocks were found positive. Among flocks tested for Salmonella spp., 2 flocks were found positive. All tested flocks were found negative for G. anatis and P. multocida as well as air sac swabs tested negative for Mycoplasma spp. Bacterial cultural examination revealed that majority of septicemic chickens were found to be infected with E. coli and these E. coli isolates showed the highest resistance to vancomycin (60%), followed by erythromycin (50%) and cefotaxime (38%) and maximum sensitivity to cefotaxime and clavulanic acid combinations (81.5%), followed by chloramphenicol (69.6%) and ertapenem (67.2%). Among the 5 avian pathogenic E. coli (APEC) virulence genes were detected in 36 flocks and highest frequency of iss (100%), followed by ompT or iutA (97.2%), hly (61.1%) and iroN (47.2%) genes. On polymerase chain reaction (PCR) screening, 10.5, 4.5, 52.2, 19.4, 9.0, 4.5, 20.1 and 19.4% of the flocks were positive for G. anatis, Ornithobacterium rhinotracheale, APEC, Salmonella spp., Mycoplasma gallisepticum, Mycoplasma synoviae, chicken infectious anemia virus and Marek's disease virus, respectively. To our knowledge, the present study is first on the etiology of septicemia in chicken flocks in India. The present study infers that the majority of septicemic deaths in broiler chickens less than 8 weeks have been connected with APEC and majority of E. coli isolates are multidrug resistance, suggesting the need for surveillance and intervention to curb the inadvertent use of antibiotics. Although, incidence of G. anatis association with septicemia was reported, still requires a rigorous epidemiological study to determine the actual prevalence. However, more detailed studies encompassing vast geographical area with large sample size and long duration of the studies are necessary to provide a clear picture of the interaction of different pathogens causing septicemia in chicken.

NO2 Gas Sensor Based on SnSe/SnSe2p-n Hetrojunction.

Air pollution is a big concern as it causes harm to human health as well as environment. NO2 can cause several respiratory diseases even in low concentration and therefore an efficient sensor for detecting NO2 at room temperature has become one of the priorities of the scientific community. Although two dimensional (2D) materials (MoS2 etc.) have shown potential for NO2 sensing at lower temperatures, but these have poor desorption kinetics. However, these limitations posed by slow desorption can be overcome, if a material in the form of a p-n junction can be suitably employed. In this work, ~150 nm thick SnSe2 thin film has been deposited by thermally evaporating in-house made SnSe2 powder. The film has been studied for its morphological, structural and gas sensing applications. The morphology of the film showed that the film consists of interconnected nanostructures. Detailed Raman studies further revealed that SnSe2 film had 31% SnSe. The SnSe-SnSe2 nanostructured sensor showed a response of ~112% towards 5 ppm NO2 at room temperature (30 °C). The response and recovery times were ~15 seconds and 10 seconds, respectively. Limit of detection for NO2 was in sub-parts per million (sub-ppm) range. The device demonstrated a better response towards NO2 compared to NH3, CH4, and H2. The mechanism of room temperature fast response, recovery and selective detection of NO2 independent of humidity conditions has been discussed based on physisorption, charge transfer, and formation of SnSe-SnSe2 (p-n) nano-junctions. Depositing a nanostructured film consisting of nano-junctions using an industrially viable thermal evaporation technique for sensing a very low concentration of NO2 is the novelty of this work.

Au/Pd Bimetallic Nanoparticles Decorated SnSe2 Thin Films for NO2 Detection.

In order to have a check and balance of the toxic gases in the environment, various kinds of sensors are currently being researched upon. As many of the toxic gases are also inflammable, therefore, there is a constant search for materials which can detect the gases at lower temperatures. Also, it is important that the sensor is selective for a particular gas. To meet such requirements, nanos-tructured materials are extensively being explored for such gas sensing applications, due to their large effective surface area. And, in order to further improve the gas sensing properties, metal catalysts are deposited over such nanomaterials. The smaller sized nanoparticles show better catalytic activity due to its effective larger surface area per unit volume. Depositing bimetallic materials is thus advantageous, since it can reduce the size of nanoparticles produced. In this work, ~7 nm thick Au/Pd thin film was sputter-coated over SnSe2 nanostructured thin films. SnSe2 thin film were deposited by thermally evaporating SnSe2 powder. The materials were characterized for their structural, morphological and gas sensing properties. The ambient temperature response for 5 parts per million (ppm) NO2 gas was measured to be 117%, with the response and recovery times being 10 and 19 seconds, respectively. The performance of the sensor improved with increase in the gas concentration and for 10 ppm gas, the recorded response was 137%, with the corresponding response and recovery times being 9 and 8 seconds, respectively. The limit of detection was 655 parts per billion (ppb). The mechanism of ambient temperature high response and low response/recovery times have been discussed based on physisorption, charge transfer, Au/Pd decoration and SnSe-SnSe2 based p-n junction. In addition, an important aspect of this work worth pointing out is the deposition of a thin film consisting of nanostructured network using an industrially viable thermal evaporation method. Thus, this work opens a new dimension for 2D materials that can be used for selective gas detection at ambient temperature.

Ovarian Cysts in the Bitch: An Update.

Functional ovarian cysts occur as solitary or multiple fluid-filled structures of variable size that are unilateral or bilateral in the bitches of age 6-8 years. Though the pathogenesis is obscure, insufficient LH surge, intrafollicular changes in gonadotrophin receptors and growth factors are the possible reasons behind the occurrence of hormonally active ovarian cysts that predisposes the bitch to the development of cystic endometrial hyperplasia-pyometra complex and occasionally hyper estrogenism. In the presence of suggestive signs, ultrasonography is the practical imaging modality for the clinical diagnosis that can be confirmed by assay of ovarian steroids and histopathology. Medical management with gonadotrophin-releasing hormone analogues and human chorionic gonadotrophin is not preferred as they are not always successful. As uterine pathologies are highly likely by the time of diagnosis, ovariohysterectomy is the treatment of choice for the follicular and luteal cysts. Understanding the cellular and molecular changes in the hypothalamo-hypophyseal ovarian axis will improve our understanding on the canine ovarian cysts.

Tin-selenide as a futuristic material: properties and applications.

SnSe/SnSe2 is a promising versatile material with applications in various fields like solar cells, photodetectors, memory devices, lithium and sodium-ion batteries, gas sensing, photocatalysis, supercapacitors, topological insulators, resistive switching devices due to its optimal band gap. In this review, all possible applications of SnSe/SnSe2 have been summarized. Some of the basic properties, as well as synthesis techniques have also been outlined. This review will help the researcher to understand the properties and possible applications of tin selenide-based materials. Thus, this will help in advancing the field of tin selenide-based materials for next generation technology.

Patho-morphological and immunohistochemical diagnosis of fibrous tissue pulmonary hamartoma in young buffaloes.

Hamartoma is a developmental disorder characterized by the presence of well-differentiated tissue, of multiple types, in excess at its normal location. The present study describes a fibrous tissue pulmonary hamartoma in two buffaloes. Grossly, there were large pale white masses present on the left diaphragmatic lobe in both cases. Microscopic examination of the lungs revealed that the bronchioles were dilated and showed many terminal respiratory bronchioles lined by a single layer of cuboidal epithelium supported by thick bands of collagenous tissue. Alveoli were distorted and separated by wide bands of connective tissues. Interalveolar septa were greatly expanded by collagen fiber proliferation and subsequent fibrosis. Fibrous connective tissues were demonstrated by immunohistochemistry (IHC) and Masson's Trichrome staining (MTS). Based on gross and histological examination, coupled with IHC and MTS, it was possible to confirm a case of hamartoma, which is rarely reported worldwide and is the first reported case from India.

A novel supramolecule-based fluorescence turn-on and ratiometric sensor for highly selective detection of glutathione over cystein and homocystein.

A cyclodextrin-based fluorescence light-up and ratiometric sensor is reported for highly selective and sensitive recognition of glutathione over cystein and homocystein. The sensing scheme developed builds up on a supramolecular assembly formed between a molecular rotor dye (ThT) and a polyanionic supramolecular host (sulfated-ß-cyclodextrin, SCD). The detection scheme is accomplished as follows: firstly, the bivalent Cu2+ quenches the emission from ThT-SCD assembly by causing the dissociation of ThT molecules from SCD surface. Secondly, when GSH is added to the copper-quenched system, owing to specific interaction between Cu2+ and GSH, Cu2+ is removed from the SCD which again allows the formation of ThT-SCD assembly. Indeed, this scheme of disassembly and reassembly successively caused by Cu2+ and GSH in the aqueous solution empowers our sensor framework to work as a good ratiometric sensor for the detection of GSH. The sensor scheme shows a linear response in the range 0-250 µM with a LOD of 2.4 ± 0.2 µM in aqueous solution and 13.6 ± 0.5 µM in diluted human serum sample. The sensor system is excited at 410 nm and the emission signal is plotted as a ratio of intensity at 545 nm (aggregate band) and 490 nm (monomer band). This ratiometric sensor system is highly selective to glutathione over cystein, homocystein, and other amino acids. Additionally, response of the sensor system towards GSH in complex biological media of serum samples demonstrates its potential for practical utility. Graphical abstract.

An ATP responsive fluorescent supramolecular assembly based on a polyelectrolyte and an AIE active tetraphenylethylene derivative.

Aggregation induced emission (AIE) active probes have attracted enormous attention due to their wide-spread and ever increasing number of applications in the sensing of chemically and biologically important molecules. AIE probes undergo drastic modulation in their photophysical features from a monomeric to aggregated state. In the current work, we report the aggregation of tetra-anionic Su-TPE (AIE active probe) in the presence of a cationic polyelectrolyte, poly(allylaminehydrochloride) (PAH). A supramolecular assembly is formed by the electrostatic interaction between cationic PAH and anionic Su-TPE molecules, which leads to drastic modulations in the spectral features of anionic Su-TPE upon addition of cationic PAH. The Su-TPE-PAH aggregate assembly has been investigated using various photophysical techniques, such as, ground-state absorption, steady-state and time-resolved emission spectroscopic techniques along with 1H NMR measurements. The Su-TPE-PAH aggregate assembly is found to be responsive towards the ionic strength of the medium and temperature which results in drastic modulations of the spectral features of the emissive supramolecular aggregate assembly. Finally, the specific recognition of an important bioanalyte, ATP, has been achieved using the formed Su-TPE-PAH supramolecular aggregate assembly as a sensing platform which displays good selectivity and high sensitivity towards ATP. Importantly, the developed sensor platform could also function in the human serum matrix, hence, demonstrating the potential of the established sensor platform for real-life applications in near future.