RESUMO
Free heme is a highly toxic molecule for a living organism and its detoxification is a very important process, especially for carnivorous animals. Here we report the discovery of a previously unknown process for neutralizing free heme in the digestive tract of domestic cats. The cornerstone of this process is the encapsulation of heme into carbonated hydroxyapatite nanoparticles, followed by their excretion with faeces. This way of heme neutralization resembles the formation of insoluble heme-containing particles in the digestive tracts of other hematophagous species (for example, the formation of insoluble hemozoin crystals in malaria-causing Plasmodium parasites). Our findings suggest that the encapsulation of heme molecules into a hydroxyapatite matrix occurs during the transition from the acidic gastric juice to the small intestine with neutral conditions. The formation of these particles and their efficiency to include heme depends on the bone content in a cat's diet. In vitro experiments with heme-hydroxyapatite nanoparticles confirm the proposed scenario.
Assuntos
Trato Gastrointestinal/metabolismo , Heme/metabolismo , Ração Animal/análise , Animais , Gatos , Dieta/veterinária , Durapatita/metabolismo , Fezes , Suco Gástrico/metabolismo , Inativação Metabólica , NanopartículasRESUMO
Lipophilic extractive metabolites from needles and defoliated twigs of Pinus armandii and P. kwangtungensis were studied by GC/MS. Needles of P. armandii contained predominantly 15-O-functionalized labdane type acids (anticopalic acid), fatty acids, nonacosan-10-ol, sterols, nonacosan-10-ol and sterol saponifiable esters, and acylglycerols, while P. kwangtungensis needles contained no anticopalic acid, but more trinorlabdane (14,15,16-trinor-8(17)-labdene-13,19-dioic acid) and other labdane type acids, nonacosan-10-ol and its saponifiable esters. The major compounds in the P. armandii defoliated twig extract were abietane and isopimarane type acids, fatty acids, sterols, labdanoids (cis-abienol), cembranoids (isocembrol and 4-epi-isocembrol), saponifiable sterol esters, and acylglycerols. The same extract of P. kwangtungensis contained larger quantities of fatty acids, caryophyllene oxide, serratanoids, sterols, saponifiable sterol esters, and acylglycerols, but lesser amounts of abietane and isopimarane type acids, cis-abienol, and lacked cembranoids. Both twig and needle extracts of P. armandii and P. kwangtungensis, as well as the extracts' fractions, significantly inhibited the growth of Gram-negative bacteria Serratia marcescens with MIC of 0.1â mg ml-1 , while in most cases they slightly stimulated the growth of Gram-positive bacteria Bacillus subtilis at the same concentrations. Thus, lipophilic extractive compounds from the needles and defoliated twigs of both pines are prospective for the development of antiseptics against Gram-negative bacteria.
Assuntos
Antibacterianos/farmacologia , Ácidos Graxos/metabolismo , Pinus/metabolismo , Extratos Vegetais/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Pinus/classificação , Especificidade da EspécieRESUMO
Here we explore the effect of the nature of organic ligands in rhenium cluster complexes [Re6 Q8 L6 ]4- (where Q=S or Se, and L=benzotriazole, 1,2,3-triazole or 1,2,4-triazole) on the biological properties of the complexes, in particular on the cellular toxicity, cellular internalization and localization. Specifically, the study describes the synthesis and detailed characterization of the structure, luminescence and electrochemical properties of the four new Re6 clusters with 1,2,3- and 1,2,4-triazoles. Biological assays of these complexes are also discussed in addition to those with benzotriazole using cervical cancer (HeLa) and immortalized human fibroblasts (CRL-4025) as model cell lines. Our study demonstrates that the presence of hydrophobic and π-bonding rich units such as the benzene ring in benzotriazole significantly enhances cellular internalization of rhenium clusters. These ligands facilitate binding of the clusters to DNA, which results in increased cytotoxicity of the complexes.
Assuntos
Complexos de Coordenação , DNA , Rênio , Triazóis , Linhagem Celular , Complexos de Coordenação/química , Complexos de Coordenação/farmacocinética , DNA/química , DNA/metabolismo , Fibroblastos , Humanos , Ligantes , Luminescência , Rênio/química , Triazóis/química , Triazóis/farmacocinética , ÁguaRESUMO
Specific molecular recognition of γ-cyclodextrin (γ-CD) by the cationic hexanuclear niobium [Nb6 Cl12 (H2 O)6 ]2+ cluster complex in aqueous solutions results in a 1:1 supramolecular assembly {[Nb6 Cl12 (H2 O)6 ]@γ-CD}2+ . NMR spectroscopy, isothermal titration calorimetry (ITC), and ESI-MS were used to study the interaction between the inorganic cluster and the organic macrocycle. Such molecular association affects the biological activity of [Nb6 Cl12 (H2 O)6 ]2+ , decreasing its cytotoxicity despite enhanced cellular uptake. The 1:1 stoichiometry is maintained in solution over a large window of the reagents' ratio, but crystallization by slow evaporation produces a 1:2 host-guest complex [Nb6 Cl12 (H2 O)6 @(γ-CD)2 ]Cl2 â 20 H2 O featuring the cluster encapsulated between two molecules of γ-CD. The 1:2 complex was characterized by XRD, elemental analysis, IR spectroscopy, and thermogravimetric analysis (TGA). Quantum chemical calculations were performed to model host-guest interaction.
Assuntos
Nióbio/química , gama-Ciclodextrinas/química , Fenômenos Biológicos , Calorimetria , Espectroscopia de Ressonância Magnética , Água/químicaRESUMO
The furocoumarin backbone is a promising platform for chemical modifications aimed at creating new pharmaceutical agents. However, the high level of biological activity of furocoumarins is associated with a number of negative effects. For example, some of the naturally occurring ones and their derivatives can show genotoxic and mutagenic properties as a result of their forming crosslinks with DNA molecules. Therefore, a particularly important area for the chemical modification of natural furocoumarins is to reduce the negative aspects of their bioactivity. By studying a group of 21 compounds-1,2,3-triazolyl modified derivatives of furocoumarin and peucedanin-using the SOS chromotest, the Ames test, and DNA-comet assays, we revealed modifications that can neutralize the structure's genotoxic properties. Theoretical aspects of the interaction of the compound library were studied using molecular modeling and this identified the leading role of the polyaromatic molecular core that takes part in stacking-interactions with the pi-systems of the nitrogenous bases of DNA.
Assuntos
Cumarínicos/química , Furocumarinas/química , Substâncias Intercalantes/química , Mutagênicos/química , Extratos Vegetais/química , Allium/citologia , Apiaceae/química , Ensaio Cometa , Quebras de DNA de Cadeia Dupla/efeitos dos fármacos , Quebras de DNA de Cadeia Simples/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Guanina/química , Ligação de Hidrogênio , Meristema/efeitos dos fármacos , Simulação de Acoplamento Molecular , Salmonella typhimurium/efeitos dos fármacosRESUMO
Synthesis of 1,2,3-triazole-substituted coumarins and also 1,2,3-triazolyl or 1,2,3-triazolylalk-1-inyl-linked coumarin-2,3-furocoumarin hybrids was performed by employing the cross-coupling and copper catalyzed azide-alkyne cycloaddition reaction approaches. The synthesized compounds were evaluated for their in vitro antibacterial activity against Staphylococcus aureus, Bacillius subtilis, Actinomyces viscosus and Escherichia coli bacterial strains. Coumarin-benzoic acid hybrids 4Ñ, 42Ñ and 3-((4-acetylamino-3-(methoxycarbonyl)phenyl)ethynyl)coumarin (29) showed promising activity against S. aureus strains, and the 1,2,3-triazolyloct-1-inyl linked coumarin-2,3-furocoumarin hybrid 37c was endowed with high selectivity against B. subtilis and E. coli species. The in vitro antibacterial activity of 4Ñ, 29, 37c and 42Ñ can potentially be compared with that of a number of modern antibiotic drugs used in the clinic, suggesting promising prospects for further research. A detailed study of the molecular interactions with the targeted protein MurB was performed using docking simulations and the obtained results are quite promising.
Assuntos
Antibacterianos/síntese química , Antibacterianos/farmacologia , Desenho de Fármacos , Furocumarinas/síntese química , Furocumarinas/farmacologia , Triazóis/síntese química , Triazóis/farmacologia , Antibacterianos/química , Bactérias/efeitos dos fármacos , Furocumarinas/química , Testes de Sensibilidade Microbiana , Simulação de Acoplamento Molecular , Termodinâmica , Triazóis/químicaRESUMO
Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series - ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B - a protein that inhibits apoptosis - is located.
Assuntos
Antineoplásicos/farmacologia , Corantes Fluorescentes/farmacologia , Isotiocianatos/farmacologia , Triterpenos/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/química , Humanos , Isotiocianatos/química , Células MCF-7 , Microscopia Confocal , Modelos Moleculares , Conformação Molecular , Relação Estrutura-Atividade , Triterpenos/química , Ácido UrsólicoRESUMO
BACKGROUND: Oxidative damage to the cell, including the formation of 8-oxoG, has been regarded as a significant factor in carcinogenesis and aging. An inbred prematurely aging rat strain (OXYS) is characterized by high sensitivity to oxidative stress, lipid peroxidation, protein oxidation, DNA rearrangements, and pathological conditions paralleling several human degenerative diseases including learning and memory deterioration. METHODS: We have used monoclonal antibodies against a common pre-mutagenic base lesion 8-oxoguanine (8-oxoG) and 8-oxoguanine DNA glycosylase (OGG1) in combination with indirect immunofluorescence microscopy and image analysis to follow the relative amounts and distribution of 8-oxoG and OGG1 in various cells of different brain regions from OXYS and control Wistar rats. RESULTS: It was shown that 8-oxoG increased with age in mature neurons, nestin- and glial fibrillary acidic protein (GFAP)-positive cells of hippocampus and frontal cortex in both strains of rats, with OXYS rats always displaying statistically significantly higher levels of oxidative DNA damage than Wistar rats. The relative content of 8-oxoG and OGG1 in nestin- and GFAP-positive cells was higher than in mature neurons in both Wistar and OXYS rats. However, there was no significant interstrain difference in the content of OGG1 for all types of cells and brain regions analyzed, and no difference in the relative content of 8-oxoG between different brain regions. CONCLUSIONS: Oxidation of guanine may play an important role in the development of age-associated decrease in memory and learning capability of OXYS rats. GENERAL SIGNIFICANCE: The findings are important for validation of the OXYS rat strain as a model of mammalian aging.
Assuntos
Senilidade Prematura/metabolismo , Envelhecimento , Dano ao DNA , Lobo Frontal/metabolismo , Guanina/análogos & derivados , Hipocampo/metabolismo , Memória , Senilidade Prematura/patologia , Animais , DNA Glicosilases/metabolismo , Lobo Frontal/patologia , Guanina/metabolismo , Hipocampo/patologia , Humanos , Proteínas de Filamentos Intermediários/metabolismo , Peroxidação de Lipídeos , Proteínas do Tecido Nervoso/metabolismo , Nestina , Neurônios/metabolismo , Neurônios/patologia , Estresse Oxidativo , Ratos , Ratos WistarRESUMO
Somatic mutations in mitochondrial DNA (mtDNA) are thought to play an important role in both aging and neurodegenerative diseases although their specific contributions remain a subject of intense debate. We analyzed somatic mutations in the mtDNA control regions in the liver of Wistar rats. The mutation rate was found to be high and increased with age from 5.3x10(-4) mutations per position to 4.48x10(-3) mutations per position at 3 and 12 months of age, respectively. The vast majority of nucleotide substitutions are transitions ( approximately 95%) with A:T>G:C transitions being the most frequent type of substitution (>50%). In 3-month-old Wistar rats, approximately 40% of somatic mutations in the control region of mtDNA are significantly consistent with the model of dislocation mutagenesis which is a signature of error-prone DNA synthesis by mtDNA polymerase gamma. The results are consistent with the previous hypothesis that normal intramitochondrial dNTP pool asymmetries, which have been shown to reduce the fidelity of mtDNA polymerase gamma, substantially contribute to somatic mutagenesis of the rat mtDNA.
Assuntos
Envelhecimento , DNA Mitocondrial , Mitocôndrias Hepáticas/genética , Mutação , Animais , Sequência de Bases , Masculino , Dados de Sequência Molecular , Ratos , Ratos WistarRESUMO
Production of free radicals in animals is accompanied with a number of pathologic conditions, some of which may be manifested through DNA damage. Studies of mechanisms of oxidative DNA damage by free radicals in vivo are hindered by the lack of good animal models with significant overgeneration of or increased sensitivity to free radicals. An inbred rat strain (OXYS) is characterized by inherited overgeneration of free radicals, lipid peroxidation, protein oxidation, DNA rearrangements, and pathological conditions paralleling several human degenerative diseases. We have used monoclonal antibodies against a common pre-mutagenic base lesion 8-oxoguanine (8-oxoG) in combination with indirect immunofluorescence microscopy and image analysis to follow the relative age-dependent amounts and distribution of 8-oxoG in liver cells from OXYS and Wistar rats. 8-OxoG increased with age in both strains of rats, with OXYS rats always displaying statistically significantly higher levels of oxidative DNA damage than Wistar rats. Statistical analysis indicates that 8-oxoG does not uniformly accumulate in all cells with advancing age or increasing free radical load, but rather concentrates in a minor fraction of cells with a high damage level.