RESUMO
BACKGROUND: This study aims to describe the factors associated with dysplastic changes in sinonasal inverted papilloma (SIP) including somatic EGFR mutation, FoxM1 expression, HPV status, and their association with dysplastic changes. METHODS: A cross-sectional, analytical study was conducted comprising 34 samples of histologically-confirmed diagnosis of SIP. The samples were further grouped into 2 groups: 20 samples without associated dysplastic changes, and 14 samples with associated dysplastic changes. The numbers of FoxM1 positively-expressed cells, EGFR mutation, and HPV status were compared among two groups using appropriate comparative statistics. RESULTS: There was statistically-significant difference of FoxM1 expression between SIP and SIP with dysplasia (10% vs 100%; p<0.001). EGFR mutation was identified in 6 samples (30.0%) of the SIP and 5 samples (35.7%) of SIP with dysplasia. No difference of EGFR mutant proportion among two groups. HPV DNA was detected in 5 samples (25.0%) of SIP versus 9 samples (64.3%) of SIP with dysplasia. There was significant difference of HPV status among two groups (p=0.022). The high-risk subtypes were found in most HPV positive samples (57.1%), while low-risk subtypes and out panel subtypes were found 14.3% and 21.4%, respectively. CONCLUSIONS: FoxM1 was overexpressed in SIP with malignant transformation. FoxM1 along with HPV status is associated with dysplastic changes in the SIP. FoxM1 immunostaining is potential to be a biomarker of malignant transformation in SIP.
Assuntos
Neoplasias Nasais , Papiloma Invertido , Infecções por Papillomavirus , Neoplasias dos Seios Paranasais , Humanos , Papiloma Invertido/genética , Papiloma Invertido/patologia , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/genética , Infecções por Papillomavirus/patologia , Neoplasias dos Seios Paranasais/genética , Neoplasias dos Seios Paranasais/patologia , Estudos Transversais , Receptores ErbB/genética , Neoplasias Nasais/genética , Neoplasias Nasais/patologia , Proteína Forkhead Box M1/genéticaRESUMO
OBJECTIVE: This study examine FOXP3, CD4, CD8 and p53 expression in the transformation of the Sinonasal Inverted Papilloma (SIP) malignancy into sinonasal carcinoma. MATERIALS AND METHODS: This study used a cross-sectional approach. The research sample from thirty-six paraffin block preparations with the diagnosis of SIP. Then, immunohistochemical staining was performed using FOXP3 mouse monoclonal antibody (236A/E7), CD8 rabbit monoclonal antibody (CD8/1179R), CD4 mouse monoclonal antibody (4B12) and p53 rabbit monoclonal antibody. Results: There was a significant difference between Foxp3 expression in SIP without dysplasia and SIP with dysplasia (p= 0.013). There was no significant difference between the expression of CD4 and CD8 in the two groups with p-values 0.1 and 0.062, respectively. The mean percentage of positive p53 expression in SIP without dysplasia was 0.45+0.63 and in the SIP with dysplasia 29.31+38.96. There was a significant difference between the two groups (p<0.001). CONCLUSION: FOXP3 and p53 were overexpressed in SIP with malignant transformation. FOXP3 together with p53 status is associated with dysplastic changed in the SIP. FOXP3 and p53 status could be potential biomarker of malignant transformation in sinonasal inverted papilloma.
Assuntos
Papiloma Invertido , Animais , Anticorpos Monoclonais , Biomarcadores , Antígenos CD4/metabolismo , Antígenos CD8/metabolismo , Transformação Celular Neoplásica/patologia , Fatores de Transcrição Forkhead/metabolismo , Humanos , Linfócitos do Interstício Tumoral/metabolismo , Papiloma Invertido/metabolismo , Papiloma Invertido/patologia , Proteína Supressora de Tumor p53/metabolismoRESUMO
Steroid cell tumours are rare type of sex cord-stromal tumour of the ovary, with the average age at diagnosis during the fourth decade of life. A 2-year-old girl presented with virilisation, precocious pseudopuberty and Cushing syndrome. Her morning cortisol level was elevated and there was left lower abdominal mass found in radiological examination. An explorative laparotomy followed by a left salpingo-oophorectomy was performed. Histopathological examination revealed an encapsulated solid yellow-tan tumour composed of polygonal cells arranged in nests/lobes, containing numerous vascular channels, with abundant, clear-to-granular eosinophilic cytoplasm, severe nuclear pleomorphism and no Reinke crystals present consistent with an ovarian steroid cell tumour (NOS). The tumours cells were diffusely positive with calretinin, weak focal positive for inhibin, melan-A, pankeratin, chromogranin and negative for AFP, HMB-45 and androgen receptors. Following the tumour removal, blood pressure regressed to normal. The patient was discharged and advised for outpatient care.