The YBX3 RNA-binding protein posttranscriptionally controls SLC1A5 mRNA in proliferating and differentiating skeletal muscle cells.

In humans, skeletal muscles comprise nearly 40% of total body mass, which is maintained throughout adulthood by a balance of muscle protein synthesis and breakdown. Cellular amino acid (AA) levels are critical for these processes, and mammalian cells contain transporter proteins that import AAs to maintain homeostasis. Until recently, the control of transporter regulation has largely been studied at the transcriptional and posttranslational levels. However, here, we report that the RNA-binding protein YBX3 is critical to sustain intracellular AAs in mouse skeletal muscle cells, which aligns with our recent findings in human cells. We find that YBX3 directly binds the solute carrier (SLC)1A5 AA transporter messenger (m)RNA to posttranscriptionally control SLC1A5 expression during skeletal muscle cell differentiation. YBX3 regulation of SLC1A5 requires the 3' UTR. Additionally, intracellular AAs transported by SLC1A5, either directly or indirectly through coupling to other transporters, are specifically reduced when YBX3 is depleted. Further, we find that reduction of the YBX3 protein reduces proliferation and impairs differentiation in skeletal muscle cells, and that YBX3 and SLC1A5 protein expression increase substantially during skeletal muscle differentiation, independently of their respective mRNA levels. Taken together, our findings suggest that YBX3 regulates AA transport in skeletal muscle cells, and that its expression is critical to maintain skeletal muscle cell proliferation and differentiation.

Characterisation of physical and mechanical properties of seven particulate materials proposed as traction enhancers.

Particulate materials are utilised in many applications to manipulate the friction between surfaces. This dataset provides the characteristics of particulates used as rail sand in the train's wheel/rail interface (via an on-board system) to facilitate the train's acceleration and deceleration. Seven materials are studied including Austrian rail sand, standard Great British rail sand, waste glass beads, recycled crushed glass, non-coated alumina, coated alumina, and dolomite. The main objective of this research is to provide a physical and mechanical characterisation of these granular materials in terms of their density, bulk behaviour, particle size, particle shape, hardness, reduced modulus, and mineralogical properties. In particular, three-dimensional raw and post-processed micro-computed tomography images of more than 1200 particles are shared. The results provide a detailed dataset which can be used in ongoing and future experimental and numerical investigations studying the role of particulates in the wheel/rail interface.

DEM modelling of railway ballast using the Conical Damage Model: a comprehensive parametrisation strategy.

Despite ongoing research, the parametrisation of a DEM model is a challenging task, as it depends strongly on the particle shape representation used, particle-particle contact law and the simulated applications: for railway ballast e.g. lab tests or track conditions. The authors previously modelled railway ballast with a DEM model using a simple particle shape. The DEM model was parametrised, by trial-and-error, to compression and direct shear test results. A good agreement between DEM model and experimental results was achieved only when the Conical Damage Model (CDM) was used as the contact law. Compared to the well-known linear-spring Cundall-Strack law or the Hertz-Mindlin law, this contact law takes into account additional physical effects (e.g. edge breakage) occurring in the experiment. Little is known on the influence of the CDM model parameters on the simulation results or on possible parameter ambiguities. This lack of knowledge hinders a reliable and efficient parametrisation of DEM models using different particle shapes. Both points are addressed in this work in detail by investigating a DEM model for railway ballast using one simple particle shape. Suggestions for a parametrisation strategy of reduced computational effort are formulated and tested using a second particle shape. In future works, the newly presented parametrisation strategy can help to calibrate different DEM models and to study the influence of particle shape.

Shape analysis of railway ballast stones: curvature-based calculation of particle angularity.

Particle shape analysis is conducted, to compare two types of railway ballast: Calcite and Kieselkalk. Focus lies on the characterisation of particle angularity using 3D scanner data. In the literature, angularity is often characterised using 2D data, as these types of data are easier to collect. 3D scanner data contain a vast amount of information (e.g. curvatures) which can be used for shape analysis and angularity characterisation. Literature approaches that use 3D data are often not thoroughly tested, due to a lack of test cases. In this work, two new curvature-based angularity indices are introduced and compared to one from the literature. Analytical test bodies with shapes ranging from spherical towards cubic are used for a first plausibility test. Then, 3D scans of ballast stones are compared to artificially rounded meshes. Only one out of three evaluated angularity indices seem to be suited to characterise angularity correctly in all of the above tests: the newly introduced scaled Willmore energy. A complete shape analysis of the scanned ballast stones is conducted and no difference between the two types of ballast can be seen regarding form, angularity, roughness, sphericity or convexity index. These findings of shape analysis are set in the context of previous works, where experimental results and DEM simulations of uniaxial compression tests and direct shear tests were presented for the same ballast types.