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OBJECTIVE: Maternal diabetes in pregnancy is associated with structural anomalies of the fetal heart, as well as hypertrophy and functional impairment. This systematic review and meta-analysis aimed to estimate the effect of maternal diabetes on fetal cardiac function as measured by prenatal echocardiography. METHODS: We performed a search of the EMBASE, PubMed and The Cochrane Library databases, from inception to 4 July 2019, for studies evaluating fetal cardiac function using echocardiography in pregnancies affected by diabetes compared with uncomplicated pregnancies. Outcome measures were cardiac hypertrophy and diastolic, systolic and overall cardiac function as assessed by various ultrasound parameters. The quality of the studies was assessed using the Newcastle-Ottawa Scale. Data on interventricular septal (IVS) thickness, myocardial performance index (MPI) and E/A ratio were pooled for the meta-analysis using random-effects models. For pregnancies with diabetes, results were reported overall and according to whether diabetes was pregestational (PDM) or gestational (GDM). Results were also stratified according to the trimester in which fetal cardiac assessment was performed. RESULTS: Thirty-nine studies were included, comprising data for 2276 controls and 1925 women with pregnancy affected by diabetes mellitus (DM). Of these, 1120 had GDM, 671 had PDM and in 134 cases diabetes type was not specified. Fetal cardiac hypertrophy was more prevalent in diabetic pregnancies than in non-diabetic controls in 21/26 studies, and impaired diastolic function was observed in diabetic pregnancies in 22/28 studies. The association between DM and systolic function was inconsistent, with 10/25 studies reporting no difference between cases and controls, although more recent studies measuring cardiac deformation, i.e. strain, did show decreased systolic function in diabetic pregnancies. Of the studies measuring overall fetal cardiac function, the majority (14/21) found significant impairment in diabetic pregnancies. Results were similar when stratified according to GDM or PDM. These effects were already present in the first trimester, but were most profound in the third trimester. Meta-analysis of studies performed in the third trimester showed, compared with controls, increased IVS thickness in both PDM (mean difference, 0.75 mm (95% CI, 0.56-0.94 mm)) and GDM (mean difference, 0.65 mm (95% CI, 0.39-0.91 mm)) pregnancies, decreased E/A ratio in PDM pregnancies (mean difference, -0.09 (95% CI, -0.15 to -0.03)), no difference in E/A ratio in GDM pregnancies (mean difference, -0.01 (95% CI, -0.02 to 0.01)) and no difference in MPI in either PDM (mean difference, 0.04 (95% CI, -0.01 to 0.09)) or GDM (mean difference, 0.03 (95% CI, -0.01 to 0.06)) pregnancies. CONCLUSIONS: The findings of this review show that maternal diabetes is associated with fetal cardiac hypertrophy, diastolic dysfunction and overall impaired myocardial performance on prenatal ultrasound, irrespective of whether diabetes is pregestational or gestational. Further studies are needed to demonstrate the relationship with long-term outcomes. © 2020 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
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Diabetes Gestacional/fisiopatologia , Ecocardiografia , Coração Fetal/fisiopatologia , Gravidez em Diabéticas/fisiopatologia , Ultrassonografia Pré-Natal , Adulto , Diabetes Gestacional/diagnóstico por imagem , Feminino , Coração Fetal/diagnóstico por imagem , Humanos , Gravidez , Trimestres da Gravidez , Gravidez em Diabéticas/diagnóstico por imagemRESUMO
OBJECTIVE: This study focuses on the evolution of treatment techniques for aortic coarctation in children and assesses long-term morbidity. METHODS: This retrospective cohort study evaluates patients treated for native aortic coarctation, with at least 7 years of follow-up. To assess time-related changes, three time periods were distinguished according to year of primary intervention (era 1, 2 and 3). Operative and long-term follow-up data were collected by patient record reviews. RESULTS: The study population consisted of 206 patients (177 surgical and 29 catheter-based interventions), with a median follow-up of 151 months. Anterior approach with simultaneous repair of aortic arch and associated cardiac lesions was more common in the most recent era. Median age at intervention did not change over time. Reintervention was necessary in one third of the cohort with an event-free survival of 74% at 5year and 68% at 10-year follow-up. Reintervention rates were significantly higher after catheter-based interventions compared with surgical interventions (hazard ratio [HR] 1.8, 95% confidence interval [CI] 1.04-3.00, pâ¯= 0.04) and in patients treated before 3 months of age (HR 2.1, 95% CI 1.27-3.55, pâ¯= 0.003). Hypertension was present in one out of five patients. CONCLUSION: Nowadays, complex patients with associated cardiac defects and arch hypoplasia are being treated surgically on bypass, whereas catheter-based intervention is introduced for non-complex patients. Reintervention is common and more frequent after catheter-based intervention and in surgery under 3 months of age. One fifth of the 206 patients remained hypertensive.
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INTRODUCTION: Congenital atrioventricular block (CAVB) is a rare disorder with a significant morbidity and mortality. Consensus regarding the prescription and efficacy of prenatal corticosteroids is lacking. This nationwide study was initiated to evaluate the effects of prenatal treatment with corticosteroids on the outcome of CAVB in The Netherlands. METHODS: All fetuses identified with isolated congenital AVB-II° or AVB-III° in any of the eight academic fetal heart centers of The Netherlands between 2003 and 2013 were included and reviewed. RESULTS: Fifty-six fetuses were included. Fourteen (25%) fetuses were treated with dexamethasone. We found no differences between the steroid-treated and untreated cases regarding in utero progression of the AVB (63% vs 67% respectively), survival to birth (86% vs 84%), pacemaker implantations (74% vs 58%) or long-term dilated cardiomyopathy (13% vs 17%). Steroid treated fetuses demonstrated more in utero growth restriction (38% vs 11%). CONCLUSION: No benefit from prenatal corticosteroid treatment was demonstrated for fetuses with isolated CAVB in this study. However, we found negative side effects. Our data provide no evidence to support the routine administration of corticosteroids for the treatment of fetal CAVB.
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Bloqueio Atrioventricular/diagnóstico por imagem , Bloqueio Atrioventricular/tratamento farmacológico , Coração Fetal/efeitos dos fármacos , Coração Fetal/diagnóstico por imagem , Esteroides Fluorados/administração & dosagem , Adulto , Bloqueio Atrioventricular/epidemiologia , Feminino , Seguimentos , Humanos , Países Baixos/epidemiologia , Gravidez , Diagnóstico Pré-Natal/métodos , Estudos Prospectivos , Resultado do TratamentoRESUMO
Pulmonary function and nutritional status are important determinants of exercise capacity in patients with cystic fibrosis (CF). Studies investigating the effects of determinants, such as genotype or infection and inflammation, are scarce and have never been analysed in a multivariate longitudinal model. A prospective longitudinal cohort study was performed to evaluate whether genotype, chronic inflammation and infection were associated with changes in exercise capacity. Furthermore, we investigated whether exercise capacity can predict clinical outcome. 504 exercise tests of 149 adolescents with CF were evaluated. Maximal oxygen uptake corrected for body mass % predicted declined 20% during adolescence, and was associated with immunoglobulin (Ig)G levels and chronic Pseudomonas aeruginosa infection. A lower exercise capacity was associated with a higher mortality, steeper decline in pulmonary function and greater increase in IgG levels. Since a decline in exercise capacity during adolescence was negatively associated with IgG levels and chronic P. aeruginosa infection, these data emphasise the importance of prevention and treatment of chronic inflammation and infections in patients with CF. Furthermore, a lower exercise capacity was associated with a higher mortality rate, steeper decline in pulmonary function and higher increase in IgG levels with increasing age in adolescents with CF. This stresses the value of regular exercise testing for assessing prognosis in adolescents with CF.
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Fibrose Cística/mortalidade , Fibrose Cística/fisiopatologia , Tolerância ao Exercício/fisiologia , Pneumonia/mortalidade , Pneumonia/fisiopatologia , Adolescente , Criança , Doença Crônica , Fibrose Cística/imunologia , Bases de Dados Factuais , Exercício Físico/fisiologia , Teste de Esforço , Feminino , Humanos , Imunoglobulina G/sangue , Estudos Longitudinais , Masculino , Consumo de Oxigênio/fisiologia , Pneumonia/microbiologia , Prognóstico , Estudos Prospectivos , Infecções por Pseudomonas/mortalidade , Infecções por Pseudomonas/fisiopatologia , Pseudomonas aeruginosaRESUMO
BACKGROUND: Siblings with cystic fibrosis (CF) share many genetic and environmental factors but may present different phenotypes. Younger sibs are mostly earlier diagnosed with CF than their older sibs, but might be at risk for an earlier colonization with Pseudomonas aeruginosa (PA) than their older counterparts due to cross-infection within families. AIMS: To analyze the effects of birth order and age at diagnosis on lung function, PA colonization, nutritional status, and survival during the first two decades of life in siblings with CF. METHODS: A retrospective cohort study of 52 sibling pairs was performed in two Dutch CF centers. Data were analyzed both cross-sectionally and longitudinally using Kaplan-Meier curves and modified log-rank tests. RESULTS: Median age at diagnosis was significantly higher in the older sib compared with the younger sib (3.0 and 0.2 years, respectively, P < 0.0001). At the age of 5, 10, and 15 years no difference in lung function was found. However, at the age of 20 years, forced expiratory volume in 1 sec (FEV(1)) in older sibs was 19.4% (95% CI: 5.9-32.9%, P = 0.007) lower than in younger sibs. In the younger sibs group, FEV(1) at age 20 years was significantly better in those who had a diagnosis before the age of 6 months (difference 22.9%, 95% CI: 0.1-45.8%, P < 0.05). In the first 10 years of life the younger sibs tended to be earlier colonized with PA than their older counterparts. No differences in nutritional status and survival were observed. CONCLUSION: In this sibling cohort study, an early diagnosis of CF was associated with better lung function after two decades of life. Although younger siblings tended to be colonized with PA at an earlier age, they showed better lung function outcomes. This underscores the importance of early diagnosis with newborn screening and early referral to a specialized center in the prevention of long-term deleterious effects on lung function.
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Ordem de Nascimento , Fibrose Cística/diagnóstico , Irmãos , Adolescente , Idade de Início , Criança , Pré-Escolar , Estudos de Coortes , Fibrose Cística/epidemiologia , Fibrose Cística/genética , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Volume Expiratório Forçado , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Masculino , Países Baixos , Infecções por Pseudomonas/epidemiologia , Pseudomonas aeruginosa , Testes de Função Respiratória , Estudos Retrospectivos , Tempo , Adulto JovemRESUMO
BACKGROUND: Long-term clinical trials have shown that daily treatment with recombinant human deoxyribonuclease (rhDNAse) in patients with mild to moderate cystic fibrosis (CF) improves lung function and decreases the number of respiratory exacerbations. The aim of this study was to analyze the effect of rhDNAse on the bacterial colonization of the airways in children with CF. METHODS: This was a retrospective cohort study. From the database of the CF Center Utrecht, we selected two groups, an rhDNAse group (daily 2.5 mg rhDNAse) and a control group (no rhDNAse). Primary outcome parameter was the difference in change in bacterial colonization between the treatment and control group during 1.5-year. Secondary outcome parameters were changes in lung function (FEV1) and pulmonary exacerbations. RESULTS: Children treated with rhDNAse showed no significant changes in bacterial colonization during the treatment period, apart from an increase of P. aeruginosa positive cultures, both compared to baseline (53.1% versus 25%, p < 0.05) and control group (no change during study period, 37% versus 37%). The change in FEV1 after one year of treatment was +4.0% in the treatment group versus -0.3% in the control group (p = 0.22). There were no significant changes in number of pulmonary exacerbations. CONCLUSIONS: This study showed no significant beneficial decrease in bacterial airway colonization during 1.5-year of treatment with rhDNAse. The positive effects of rhDNAse on the lung function can therefore not be explained by a change in airway colonization.
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Portador Sadio/prevenção & controle , Fibrose Cística/tratamento farmacológico , Fibrose Cística/microbiologia , Desoxirribonucleases/uso terapêutico , Proteínas Recombinantes/uso terapêutico , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Volume Expiratório Forçado , Humanos , Lactente , Masculino , Infecções por Pseudomonas/prevenção & controle , Pseudomonas aeruginosa/isolamento & purificação , Estudos RetrospectivosRESUMO
In Cftr-/- mice that mostly die because of intestinal obstruction, intestinal expression of Clca3 is decreased, whereas upregulation of Clca3 results in amelioration of intestinal disease. The aim of the study was to investigate whether the p.S357N variant in CLCA1, the human orthologue of Clca3, acts as a modifier gene in a cohort of 682 European patients with cystic fibrosis (CF)-99 patients with meconium ileus. The 357SS genotype was significantly overrepresented in both patients with meconium ileus and also with a severe CFTR genotype (P = 0.009) and in p.F508del homozygotes (P = 0.002). This suggests that CLCA1 has similar important functions in CF-related intestinal obstruction in humans as in Cftr-/- mice.
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Canais de Cloreto/genética , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Fibrose Cística/genética , Variação Genética , Íleus/genética , Adolescente , Adulto , Criança , Fibrose Cística/complicações , Europa (Continente) , Feminino , Genótipo , Humanos , Íleus/complicações , Recém-Nascido , Masculino , Mecônio , Adulto JovemRESUMO
BACKGROUND: Serological methods to monitor Pseudomonas aeruginosa colonisation in patients with cystic fibrosis (CF) are advocated but the diagnostic value of a commercially available P aeruginosa antibody test to detect early and chronic P aeruginosa colonisation in a non-research setting has not been assessed. METHODS: Colonisation with P aeruginosa was estimated by regular culture of sputum or oropharyngeal swabs during three consecutive years in 220 patients with CF aged 0-65 years. Commercially available ELISA tests with three P aeruginosa antigens (elastase, exotoxin A, alkaline protease) were performed at the end of the study period. In a subgroup of 57 patients (aged 4-14 years) serological tests were performed annually. RESULTS: Using culture as the reference standard, the ELISA tests using the advised cut off values had a sensitivity of 79% and a specificity of 89% for chronic colonisation. Receiver-operator characteristic curves were created to optimise cut off values. Applying these new cut off values resulted in a sensitivity of 96% and a specificity of 79%. All three individual serological tests discriminated well between the absence and presence of chronic P aeruginosa colonisation. The sensitivity of the individual antibody test was 87% for elastase, 79% for exotoxin A, and 76% for alkaline protease. First colonisation was preceded by positive serological results in only five of 13 patients (38%). CONCLUSION: In patients with CF, serological tests using specific antigens are sensitive for diagnosing chronic P aeruginosa colonisation. However, the failure of serological tests to detect early colonisation in young patients emphasises the need for continued reliance on cultures.
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Anticorpos Antibacterianos/sangue , Fibrose Cística/microbiologia , Infecções por Pseudomonas/diagnóstico , Pseudomonas aeruginosa/imunologia , Adulto , Idoso , Antígenos de Bactérias , Ensaio de Imunoadsorção Enzimática/normas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Padrões de Referência , Sensibilidade e EspecificidadeRESUMO
Although the measurement of peak expiratory flow (PEF) is frequently used in general practice as a surrogate for forced expiratory volume in one second (FEV1) in the assessment of airway obstruction and bronchodilator response (BDR), its use has never been validated in children with asthma. Spirometry and PEF measurements (mini-Wright peak flow meter) were performed in 271 children with asthma who attended the hospital for a routine pulmonary evaluation. Airway obstruction was defined as FEV1 as a percentage of predicted (FEV1% pred) < 80%; a positive BDR was defined as an increase in FEV1% pred > or 9% after inhaling 800 micrograms salbutamol. The Spearman correlation coefficient between the percent-predicted values of PEF (PEF% pred) and FEV1% pred was 0.36, Commonly used cut off values for airway obstruction of PEF% pred < 75% and PEF% pred < 80% had a high specificity (95%, 91%) and NPV (95%, 95%), but a moderate sensitivity (54%, 57%) and PPV (54%, 41%). After administration of the bronchodilator, the Spearman correlation coefficient between the different expressions of delta PEF and delta FEV1% pred ranged between 0.52 and 0.54. Commonly used cut off values for BDR of delta PEF% init (increase in PEF as percentage of initial value) > or = 20% and delta PEF% init > or = 25% had a high specificity (96%, 96%), a reasonable NPV (74%, 69%) and PPV (74%, 85%), but a moderate sensitivity (51%, 53%). In conclusion, PEF testing has the properties to be a good screening test to exclude airway obstruction and BDR (high specificity and NPV), but is of less clinical value as a diagnostic test (moderate sensitivity and PPV).