Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics.

Introduction: Humic substances (HSs), components of plant biostimulants, are known to influence plant physiological processes, nutrient uptake and plant growth, thereby increasing crop yield. However, few studies have focused on the impact of HS on overall plant metabolism, and there is still debate over the connection between HS' structural characteristics and their stimulatory actions. Methods: In this study, two different HSs (AHA, Aojia humic acid and SHA, Shandong humic acid) screened in a previous experiment were chosen for foliar spraying, and plant samples were collected on the tenth day after spraying (62 days after germination) to investigate the effects of different HSs on photosynthesis, dry matter accumulation, carbon and nitrogen metabolism and overall metabolism in maize leaf. Results and discussion: The results showed different molecular compositions for AHA and SHA and a total of 510 small molecules with significant differences were screened using an ESI-OPLC-MS techno. AHA and SHA exerted different effects on maize growth, with the AHA inducing more effective stimulation than the SHA doing. Untargeted metabolomic analysis revealed that the phospholipid components of maize leaves treated by SHA generally increased significantly than that in the AHA and control treatments. Additionally, both HS-treated maize leaves exhibited different levels of accumulation of trans-zeatin, but SHA treatment significantly decreased the accumulation of zeatin riboside. Compared to CK treatment, AHA treatment resulted in the reorganization of four metabolic pathways: starch and sucrose metabolism, TCA cycle, stilbenes, diarylheptanes, and curcumin biosynthesis, and ABC transport, SHA treatment modified starch and sucrose metabolism and unsaturated fatty acid biosynthesis. These results demonstrate that HSs exert their function through a multifaceted mechanism of action, partially connected to their hormone-like activity but also involving hormoneindependent signaling pathways.

Response of different varieties of maize to nitrogen stress and diagnosis of leaf nitrogen using hyperspectral data.

Spectral technology is theoretically effective in diagnosing N stress in maize (Zea mays L.), but its application is affected by varietal differences. In this study, the responses to N stress, leaf N spectral diagnostic models and the differences between two maize varieties were analysed. The variety "Jiyu 5817" exhibited a greater response to different N stresses at the 12-leaf stage (V12), while "Zhengdan 958" displayed a greater response in the silking stage (R1). Correlation analysis showed that the spectral bands more sensitive to leaf N content were 548-556 nm and 706-721 nm at the V12 stage in "Jiyu 5817" and 760-1142 nm at the R1 stage in "Zhengdan 958". An N spectral diagnostic model that considers the varietal effect improves the model fit and root mean square error (RMSE) with respect to the model without it by 10.6% and 29.2%, respectively. It was concluded that the V12 stage for "Jiyu 5817" and the R1 stage for "Zhengdan 958" were the best diagnostic stages and were more sensitive to N stress, which can further guide fertilization decision-making in precision fertilization.

Analysis of metabolic differences in maize in different growth stages under nitrogen stress based on UPLC-QTOF-MS.

Introduction: Maize has a high demand for nitrogen during the growth period. The study of metabolic changes in maize can provide a theoretical basis for rational nitrogen nutrition regulation. Methods: In order to investigate the changes of different metabolites and their metabolic pathways in maize leaves under nitrogen stress, we used ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) for metabolomic analysis of maize leaves under different nitrogen treatments at three critical growth stages (V4, V12 and R1) in a pot experiment under natural conditions. Results and discussion: The results showed that nitrogen stress significantly affected sugar metabolism and nitrogen metabolism, and affected carbon and nitrogen balance, and the effects of stress on maize leaves metabolism increased with the growth process. Metabolic pathways such as the TCA cycle and starch and sucrose metabolism were mainly affected at the seeding stage (V4). The stress response to nitrogen deficiency also showed significant upregulation of flavonoids such as luteolin and astragalin during the booting stage (V12) and anthesis-silking stage (R1). During R1 stage, the synthesis of tryptophan and phenylalanine and the degradation of lysine were significantly affected. Compared with nitrogen stress, the metabolic synthesis of key amino acids and jasmonic acid were intensified and the TCA cycle was promoted under nitrogen sufficiency conditions. This study initially revealed that the response mechanism of maize to nitrogen stress at the metabolic level.