Investigating the Mechanism of Cadmium-Tolerant Bacterium Cellulosimicrobium and Ryegrass Combined Remediation of Cadmium-Contaminated Soil.

Cadmium (Cd) pollution has been rapidly increasing due to the global rise in industries. Cd not only harms the ecological environment but also endangers human health through the food chain and drinking water. Therefore, the remediation of Cd-polluted soil is an imminent issue. In this work, ryegrass and a strain of Cd-tolerant bacterium were used to investigate the impact of inoculated bacteria on the physiology and biochemistry of ryegrass and the Cd enrichment of ryegrass in soil contaminated with different concentrations of Cd (4 and 20 mg/kg). The results showed that chlorophyll content increased by 24.7% and 41.0%, while peroxidase activity decreased by 56.7% and 3.9%. In addition, ascorbic acid content increased by 16.7% and 6.3%, whereas glutathione content decreased by 54.2% and 6.9%. The total Cd concentration in ryegrass increased by 21.5% and 10.3%, and the soil's residual Cd decreased by 86.0% and 44.1%. Thus, the inoculation of Cd-tolerant bacteria can improve the antioxidant stress ability of ryegrass in Cd-contaminated soil and change the soil's Cd form. As a result, the Cd enrichment in under-ground and above-ground parts of ryegrass, as well as the biomass of ryegrass, is increased, and the ability of ryegrass to remediate Cd-contaminated soil is significantly improved.

Inoculation of cadmium-tolerant bacteria to regulate microbial activity and key bacterial population in cadmium-contaminated soils during bioremediation.

The perennial ryegrass Lolium perenne can be used in conjunction with cadmium (Cd)-tolerant bacteria such as Cdq4-2 (Enterococcus spp.) for bioremediation of Cd-contaminated soil. In this study, a theoretical basis was provided to increase the efficiency of L. perenne remediation of Cd-contaminated soil using microorganisms to maintain the stability of the soil microbiome. The experimental design involved three treatment groups: CK (soil without Cd addition) as the control, 20 mg·kg-1 Cd-contaminated soil, and 20 mg·kg-1 Cd-contaminated soil + Cdq4-2, all planted with L. perenne. The soil was collected on day 60 to determine the soil microbial activity and bacterial community structure and to analyze the correlation between soil variables, the bacterial community, available Cd content in the soil, Cd accumulation, and L. perenne growth. The soil microbial activity and bacterial community diversity decreased under Cd stress, and the soil microbial community composition was changed; while inoculation with Cdq4-2 significantly increased soil basal respiration and the activities of urease, invertase, and fluorescein diacetate (FDA) hydrolase by 83.65%, 79.72%, 19.88%, and 96.15% respectively; and the stability of the community structure was also enhanced. The Actinobacteriota biomass, the amount of available Cd, and the above- and belowground Cd content of L. perenne were significantly negatively correlated with the total phosphorus, total potassium, and pH. The activity of urease, invertase, and FDA hydrolase were significantly positively correlated with the biomasses of Acidobacteriota and L. perenne and significantly negatively correlated with the Chloroflexi biomass. Further, the available soil Cd content and the above- and belowground Cd levels of L. perenne were significantly positively correlated with the Actinobacteriota biomass and significantly negatively correlated with the Gemmatimonadetes biomass. Overall, inoculating Cd-tolerant bacteria improved the microbial activity, diversity, and abundance, and changed the microbial community composition, facilitating the remediation of Cd-contaminated soil by L. perenne.

Effects of biochar on the physiology and heavy metal enrichment of Vetiveria zizanioides in contaminated soil in mining areas.

The effects of biochar addition on the physiological and biochemical characteristics of Vetiveria zizanioides, and the enrichment of heavy metals, were studied herein. The aim was to provide a theoretical reference for biochar to regulate the growth of V. zizanioides in the heavy metal-contaminated soil of mining areas and the enrichment capacity of Cu, Cd, and Pb. The results showed that the addition of biochar significantly increased the contents of various pigments in the middle and late growth stages of V. zizanioides, reduced the contents of malondialdehyde (MDA) and proline (Pro) in each growth period, weakened the peroxidase (POD) activity during the entire growth period; superoxide dismutase (SOD) activity decreased in the initial stages and substantially increased in the middle and late stages. The addition of biochar reduced the enrichment of Cu in the roots and leaves of V. zizanioides, while the enrichment of Cd and Pb increased. In conclusion, it was found that biochar could reduce the toxicity of heavy metals in contaminated soil in the mining area, affect the growth of V. zizanioides and its accumulation of Cd and Pb, and is, therefore, beneficial to the restoration of contaminated soil and the overall ecological restoration of the mining area.

Effects of applying peanut shell and its biochar on the microbial activity and community structure of dryland red soil.

Due to its soil formation process, dryland red soil has certain characteristics that are unfavorable for crop growth, including acidity, fineness, plate structures, and erosivity. The use of large amounts of fertilizer can decrease fertility and biodiversity and increase acidification, thereby seriously restricting the sustainable utilization of dryland red soil resources. Therefore, there is an urgent need for techniques that improve the crop quality and yield in dryland red soil areas. Returning crop waste to fields as fertilizer is a promising approach to sustainable agriculture. In the present study, the effects of applying peanut shell and an associated biochar product to dryland red soil were investigated, with a focus on soil microbial activity and community structure. Field experiments were conducted in Jiangxi Province, southern China, in 2020, in field plots of sweet potato crops. Seven treatments were set up according to the principle of equal carbon return to farmland: Control: (conventional fertilization); S1, S2, S3 (peanut shell application of 3000, 4500 and 6000 kg hm-2, respectively); and BC1, BC2, BC3 (peanut shell biochar application of 1000, 1500 and 2000 kg hm-2, respectively). The application of peanut shell and its biochar improved soil basal respiration, with the greatest increase relative to controls of 161.06% found in treatment S3 at the root harvest stage. The most obvious increase in microbial biomass carbon content due to biochar application was 206.50% in treatment BC2 at the root harvest stage. The application of peanut shell and its biochar increased the phospholipid fatty acid (PLFA) contents of total soil microorganisms and different microbial groups. The maximum increases in the PLFA contents of total soil microorganisms, gram-positive bacteria, and gram-negative bacteria occurred at the early root formation stage in treatment BC2, which were 112.16%, 102.52%, and 115.64%, respectively. Both peanut shell and biochar increased the PLFA contents of soil actinomycetes, arbuscular mycorrhizal fungi (AMF), and other fungi to certain extents. The soil actinomycetes PLFAs increased by 120.08% at the early root formation stage in BC2, while the AMF PLFAs increased by 79.44% at the seedling stage in S2. This study provides theoretical and practical guidance for the comprehensive utilization of peanut shell and the implementation of circular agriculture in dryland red soil regions. It also provides a scientific basis for improving the fertility of dryland red soil.

Dynamic mechanisms of cadmium accumulation and detoxification by Lolium perenne grown in soil inoculated with the cadmium-tolerant bacterium strain Cdq4-2.

Cadmium (Cd) contamination is a serious threat to food security and human health. The cost-effective in situ method of remediating Cd-contaminated soil uses Cd-tolerant microorganisms and Cd-enriching plants. The present study investigated the dynamic effects of inoculating soil with a Cd-tolerant bacteria strain Cdq4-2 (Enterococcus sp.) on the physiological and biochemical properties of perennial ryegrass Lolium perenne. The combined effects of remediating Cd-contaminated soil with this plant and these bacteria were also studied. An experiment was used to compare three treatments of L. perenne crops: 1) CK (control soil without Cd), 2) C (20 mg/kg Cd-contaminated soil), and 3) CB (20 mg/kg Cd-contaminated soil inoculated with bacteria Cdq4-2). The results show that compared with treatment C, the aboveground biomass, underground biomass, and total biomass of CB were 46.83-69.31%, 131.76-462.79%, and 62.65-101.53% greater, respectively. The superoxide dismutase activity of CB was 17.62-54.63% lower, while its peroxidase activity was 67.49-146.51% higher. The malondialdehyde concentration in CB was 30.40-40.24% more significant, the ascorbic acid concentration was 6.20-188.22% higher, and its glutathione concentration was 16.25-63.63% lower. The Cd concentrations of aboveground parts of a plant in treatment CB were 18.55% and 30.53% higher than those of C at days 20 and 40, respectively, while that of underground parts was 24.25% higher on day 40. The bioconcentration factors of aboveground and underground parts were higher in treatment CB on day 40. The inoculation of Cd-contaminated soils with bacteria Cdq4-2 promoted growth in L. perenne, improved its antioxidant ability, and promoted the absorption, translocation, and accumulation of Cd. Hence, it improved the effectiveness of L. perenne in remediating Cd-contaminated soils.