RESUMO
Zinc insufficiency is a nutritional trouble worldwide, especially in developing countries. In the current study, an experiment was conducted to evaluate the effect of supplementation of MS media culture with different concentrations of ZnO nanoparticles (NPs) (0, 10, 20, 40, 80, and 160 ppm) on growth, nutrient uptake, and some physiological parameters of 7-days-old mung bean seedlings. ZnO NPs enhanced the Zn concentration of mung bean from 106.41 in control to more than 4600 µg/g dry weight in 80 and 160 ppm ZnO NPs treated seedlings. Our results showed that ZnO NPs in the concentration range from 10 to 20 ppm had a positive influence on growth parameters and photosynthetic pigments. Higher levels of ZnO NPs negatively affected seedling's growth by triggering oxidative stress which in turn caused enhancing antioxidative response in seedlings including polyphenol oxidase and peroxidase activity as well as phenolic compounds and anthocyanine contents. Considering the positive effects of ZnO NPs treatment on mungbean seedlings growth, micronutrents, protein and shoot phenolics content, 20 ppm is recommended as the optimal concentration for biofortification. Our findings confirm the capability of ZnO NPs in the remarkable increase of Zn content of mungbean seedlings which can be an efficient way for plant biofortification and dealing with environmental stress. Supplementary information: The online version contains supplementary material available at 10.1007/s11756-022-01269-3.
RESUMO
Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. To elucidate the molecular response of mangrove plants to crude oil-sediment mixture, seeds of Avicennia marina were planted and grown on 0, 2.5, 5.0, 7.5 and10 % (w/w) oil-contaminated soil. Plant biomass was highly affected from 3.05 ± 0.28 (Control) to 0.50 ± .07 (10 %) and from 3.47 ± 0.12 to 1.88 ± 0.08 in 2 and 4 months old plants respectively. The expression analysis of 11genes belonging to detoxification pathways in the roots and leaves of 2 and 4 month-old plants was evaluated by qRT-PCR. Our results showed changes in expression levels of Fe-SOD, Mn-SOD, CAT, PRX, PPOs, GSTs, and NAP2 whose products are involved in reactive oxygen species (ROS) and xenobiotic detoxification. PPOA showed the highest expression induction of 43 ± 1.15, followed by CAT (12.61 ± 3.25) and PPOB (6.38 ± 1.34) in leaves of 2 months old seedlings grown on 7.5, 10 and 7.5 % oil contaminated soil respectively. PPOA (39.23 ± 2.1), PRX (32.13 ± 1.2) as well as PPOB (26.11 ± 1.3) showed the highest expression induction in leaves of 4 months old plants grown in 2.5 % oil contaminated soil. Our data indicated that PPOA can be a good biomarker candidate gene for long term exposure to oil contamination in A. marina.
RESUMO
Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use, especially for medicinal plants. We sought to extend our knowledge of the Lavandula angustifolia genome and molecular basis of its salinity tolerance by using cDNA amplified fragment length polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl. All identified transcript derived fragments (TDF) were assigned as novel L. angustifolia genes related to signal transduction, regulation of gene expression, alternative splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to different concentrations of NaCl revealed various levels of mRNA of the identified genes in this plant. Our findings provided primary insights into the molecular response of L. angustifolia to salinity.