RESUMO
A high-performance liquid chromatographic method has been developed for the determination of nitroxoline in 50-microliter plasma and urine samples. A structural analogue of nitroxoline, 8-hydroxyquinoline, was added to the eluent in order to suppress peak asymmetry. Several parameters of the eluent were studied for the optimisation of the chromatographic system. Plasma concentration-time curves were constructed for three volunteers after they had received an oral dose of 100 mg of nitroxoline. Plasma half-life was about 1 h. Within 12 h, about 1% of the dose was excreted in the urine as free nitroxoline and about 30% as conjugated metabolite of the parent compound.
Assuntos
Anti-Infecciosos Urinários/sangue , Nitroquinolinas/sangue , Anti-Infecciosos Urinários/urina , Cromatografia Líquida de Alta Pressão/métodos , Humanos , Concentração de Íons de Hidrogênio , Nitroquinolinas/urina , Fatores de TempoRESUMO
A rapid high-performance liquid chromatographic method has been developed for monitoring plasma level of patients treated intravenously with nalidixic acid. The major metabolite (in vitro also active) can be determined as well; 50 microliter plasma samples are sufficient. Use is made of a dynamic anion-exchange system. Different parameters such as adsorption of the surfactant cetrimide onto the column; pH and ionic strength of the eluent, and the critical micelle concentration of the surfactant in the eluent have been studied.