Assuntos
Doenças Transmissíveis Emergentes/microbiologia , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/classificação , beta-Lactamases/biossíntese , Adulto , Idoso , Técnicas de Tipagem Bacteriana , Doenças Transmissíveis Emergentes/epidemiologia , Conjugação Genética , Grécia/epidemiologia , Humanos , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Masculino , Testes de Sensibilidade Microbiana , Transformação Bacteriana , beta-Lactamases/genéticaRESUMO
The systemic exposure of plum tree growers and operators to plant protection products (PPPs) and effects on DNA were assessed. Specifically, a GC-MS/MS method was developed and validated for the analysis of serum samples for the presence of seven active substances of PPPs. The analytical results verified the presence of myclobutanil, propargite, cypermethrin and deltamethrin in 7 out of 19 serum samples. The incidence of DNA damage was monitored using the single cell electrophoresis assay (comet assay). A paired Student's t-test revealed a statistically significant increase of SSBs in the blood samples collected at the end of the cropping period as compared to the samples collected from the same subjects before the start of PPPs application period. Moreover, the group of seven subjects with detectable serum pesticides levels revealed statistically significant increase of SSBs as compared to the group of subjects with no detectable PPP levels. The results of the present study demonstrate that the agriculture workers may exhibit detectable level of systemic exposure to the applied PPPs which are correlated to increased DNA damage during the cultivation period.
Assuntos
Agricultura , Dano ao DNA/efeitos dos fármacos , Exposição Ocupacional/análise , Praguicidas/toxicidade , Adulto , Idoso , Ensaio Cometa , Monitoramento Ambiental/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Resíduos de Praguicidas/sangue , Praguicidas/química , Reprodutibilidade dos Testes , Espectrometria de Massas em TandemRESUMO
A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-mass spectrometry and the recoveries ranged from 79 to 104% at two spiking levels for 6 replicates. Detection and quantification limits were calculated as 1.51 and 4.54 ng/mL of serum respectively. Two fenthion metabolites fenoxon and fenthion-sulfoxide were also identified.