Antibacterial, antibiofilm, and antivirulence effects of nanoparticles synthesized from Colletotrichum gloeosporioides in pathogenic E.coli.

Antimicrobial resistance is a global problem and antibiotics are becoming ineffective due to the resistance developed by bacteria. In this current research, silver nanoparticles were synthesized from aqueous extract of endophytic fungi Colletotrichum gloeosporioides (CgAgNPs) and characterized by various methods. CgAgNPs efficacy was analyzed by performing antimicrobial assays in Escherichia coli ATCC 25922 and antibiotic resistant pathogenic strains. Upon treatment with CgAgNPs biofilm formation was reduced in all E.coli strains. In vitro cytotoxicity assays revealed that CgAgNPs were able to increase the membrane permeability and induced leakage of sugars and proteins. CgAgNPs induced oxidative stress in E. coli strains led to lipid peroxidation and release of malonaldehyde. The CgAgNPs were able to modulate the anti-oxidant system of cells hence there was a reduction in Glutathione reductase, Catalase and Superoxide dismutase enzymes activities. Analysis of expression of gene encoding CTX-M-15 showed the down regulation upon treatment with ampicillin and CgAgNPs. Overall, the results suggest that CgAgNPs control growth, biofilm formation in E. coli through induction of oxidative stress, interference with antioxidant enzymes, cell content leakage and finally downregulating the virulence gene by interfering with transcription and translation in E. coli. In future, CgAgNPs can be incorporated in formulations to break antibiotic resistance in antibiotic resistant pathogenic E. coli.

Engineered green nanoparticles interact with Nigrospora oryzae isolated from infected leaves of Arachis hypogaea.

Oilseed crop diseases are a major concern around the world, particularly in India. The synthetic fungicides not only kill the pathogen, but they also harm the host plant and beneficial microbes and on continuous usage, they decrease the soil fertility. To overcome this problem, green nanotechnology has been a greater alternative with promising benefits. The green synthesized nanoparticles from the extract of various plant parts are an effective remedy for killing the pathogens without affecting the host plants and the environment. Hence, in our study silver nanoparticles were synthesized from Fennel seed (Foeniculum vulgare) extract. The synthesis of nanoparticles was confirmed using UV-vis, Fourier transform infrared, dynamic light scattering, zeta potential, and scanning electron microscopic analysis. The in vitro antifungal study was carried out and revealed that the nanoparticles had high efficacy against the isolated phytopathogen Nigrospora oryzae which causes tikka disease in Arachis hypogaea plants. Hence, F. vulgare seed nanoparticles can be used as an effective alternative to synthetic fungicides without causing any deleterious effect on soil microflora or the environment.