In vitro chemosensitivity testing of the feline large granular lymphocyte cell line (S87).

BACKGROUND: Feline large granular lymphocyte (LGL) lymphoma is an aggressive neoplasia characterised by short survival and poor response to chemotherapy. OBJECTIVES: In this study, the effect of different chemotherapeutic agents on the growth kinetics of the feline cell line S87, a non-MHC-restricted feline LGL cell line, was investigated. Where possible, IC50 (inhibitory concentration 50) values were determined. The IC50 values of the cell line as lymphoma models can provide clues to the situation in vivo and serve as a basis for studying resistance mechanisms. METHODS: Cells were incubated with various concentrations of vincristine, doxorubicin, 4-hydroperoxycyclophosphamide, prednisolone, methotrexate and L-asparaginase for 24 and 48 h, respectively. RESULTS: The IC50 values could be determined as 14.57 (7.49-28.32) µg/mL at 24 h incubation and 5.72 (4.05-8.07) µg/mL at 48 h incubation for doxorubicin and 9.12 (7.72-10.76) µg/mL at 24 h incubation and 4.53 (3.74-5.47) µg/mL at 48 h incubation for 4-hydroperpoxycyclophosphamide. Treatment with vincristine and methotrexate resulted in relatively high cell resistance whereas L-asparaginase and prednisolone treatment led to a reduction in cell number compared to control while cell viability was not affected (cytostatic effect). CONCLUSION: Overall, the feline LGL cell line S87 proves to be relatively sensitive to doxorubicin and 4-hydroperoxycyclophosphamide and relatively resistant to treatment with vincristine, prednisolone, methotrexate and L-asparaginase. The results of this study can be used for further investigations on resistance mechanisms in feline LGL lymphoma. Doxorubicin and cyclophosphamide can be interpreted as promising candidates for the therapy of feline LGL lymphomas.

[Real-time analysis of parturition in bitches of a medium-sized breed]. / Echtzeitanalyse des Geburtsablaufes bei Hündinnen einer mittelgroßen Rasse.

OBJECTIVE: Observation of the birth processes in healthy bitches under controlled conditions. The primary aim was to gain more insight into the natural birth process. Additional goal was to determine under which circumstances caregivers seek veterinary assistance. MATERIAL AND METHODS: Data regarding length of gestation, the course of the parturition process, litter size, as well as neonatal characteristics were collected from 345 Boxer bitches. A real-time evaluation supplied the data concerning the birth process itself. Statistical analysis included single- and multi-factor variance analyses, as well as correlation, regression, and rank correlation analyses. RESULTS: Mother dogs with fewer fetuses were gravid for a significantly longer period than those with a high number (p=0.0012). The proportion of live neonates decreased significantly from the 5th litter onwards (p=0.0072). Female neonates exhibited a lower birth weight than male neonates (p<0.0001). Diurnal influences on the onset of stage II were not found. Birth processes could be divided into 3 groups based on the recorded progression: group 1 - eutocia (54.6%), group II - eutocia with prophylactic measures by the caregiver (20.5%), and group III - dystocia (24.9%). Bitches of group 1 were slightly younger than those of groups 2 and 3. In groups 2 and 3, the proportion of older primiparae (≥4 years) was significantly higher than in group 1 (p<0.05). The total duration of labor differed significantly between groups 1 and 2 (p<0.0001). Significant differences were seen between the groups in labor activity. In group 3, the proportion of bitches with a type I (=primary) weakness in labor was strikingly high (45.2%). In 83.8% of the births (groups 1 and 2), one or more pauses in labor (>60 min) occurred during the expulsive phase. This was correlated with litter size (p=0.0025), but not with age or birth number. The rate of stillbirth was positively correlated with duration of the birth process. Reasons for veterinary intervention primarily pertained to conditions of type II and III labor weakness (inadequate contractions of the uterus during parturition). The average time span between identification of a birth disorder and presentation of the bitch to a practice/clinic was 4.8±3.3 hours. CONCLUSION AND CLINICAL RELEVANCE: In pre-partum-counselling, special attention should be paid to conditions of hyperfetia (>20% above the mean) as well as uniparous and biparous gravidity and these dams should be classified as risk patients with regard to the course of parturition. In the case of birth complications, rapid veterinary intervention is warranted in order to minimize the development of maternal inanition and fetal vitality depression.

Effect of DL-Methionine Supplementation on Tissue and Plasma Antioxidant Status and Concentrations of Oxidation Products of Cholesterol and Phytosterols in Heat-Processed Thigh Muscle of Broilers.

In this study, the hypothesis that supplementation with methionine (Met) as DL-Met (DLM) in excess of the National Research Council (NRC) recommendations improves the antioxidant system in broilers was investigated. Day-old male Cobb-500 broilers (n = 72) were divided into three groups which were fed a control diet or diets supplemented with two levels of DLM in which the concentrations of Met + Cys exceeded the recommendations of NRC by 15-20% (group DLM 1) or 30-40% (group DLM 2), respectively. The three groups of broilers did not show differences in body weight gains, feed intake, and feed conversion ratio. However, broilers of groups DLM 1 and DLM 2 had higher concentrations of glutathione (GSH) in liver and thigh muscle and lower concentrations of cholesterol oxidation products (COPs) in heat-processed thigh muscle than broilers of the control group. Concentrations of several oxidation products of phytosterols in heat-processed thigh muscle were also reduced in groups DLM 1 and DLM 2; however, the concentration of total oxidation products of phytosterols was not different between the three groups. The study shows that DLM supplementation improved the antioxidant status due to an increased formation of GSH and reduced the formation of COPs during heat-processing in thigh muscle.

Diffusion-weighted imaging of the brains of dogs with idiopathic epilepsy.

BACKGROUND: Idiopathic epilepsy is one of the most common neurological disorders in dogs. Unfortunately, up to 30% of dogs with idiopathic epilepsy show no improvement under antiepileptic drug treatment. Diffusion-weighted imaging is used in human medicine to identify epileptogenic foci in the brain to allow for more invasive treatments such as deep brain stimulation or surgical removal. The aim of this study was to ass the feasibility of interictal diffusion-weighted MRI in dogs and to evaluate the distribution of diffusion in the brains of dogs with idiopathic epilepsy (IE) and to compare these values to previously published values from healthy beagle dogs. Client-owned dogs with the final diagnosis of IE were included in this study. MRI examination was carried out using a 1.0Tesla superconductive magnet. Diffusion-weighted images using a single shot echo planar imaging sequence (SSh-EPI) with a b value of b = 0 s/mm2 and b = 800 s/mm2 were acquired in a dorsal and transverse plane with diffusion gradients in all three planes (x-, y- and z-plane). An ADC (apparent diffusion coefficient) map of the isometric image of each acquired slice was generated. Regions of interest (ROIs) were manually drawn around the caudate nucleus, the thalamus, the piriform lobe including the amygdala, the hippocampus, the semioval center and the temporal cerebral cortex by one of the authors. ROI drawings were repeated 5 times at different time points to assess intra-obersver variability. A multi-way mixed-model analysis of variance (ANOVA) and two-way ANOVA were used during statistical analysis. A p value of p < 0.05 was considered significant. RESULTS: Dogs with IE showed a significantly increased ADC in the amygdala within the piriform lobe and in the semioval center (p < 0.05) compared with the healthy control group. CONCLUSION: Changes in the piriform lobe in cases of epilepsy are reported infrequently in human and veterinary medicine. Similar to our results, ADC changes in the interictal phase usually include an increase in ADC due to cell loss and increased intercellular spaces. Diffusion MRI might be a promising technique for the examination of canine epileptic patients lacking other gross neuromorphological abnormalities.