Systems genetics uncover new loci containing functional gene candidates in Mycobacterium tuberculosis-infected Diversity Outbred mice.

Mycobacterium tuberculosis, the bacillus that causes tuberculosis (TB), infects 2 billion people across the globe, and results in 8-9 million new TB cases and 1-1.5 million deaths each year. Most patients have no known genetic basis that predisposes them to disease. We investigated the complex genetic basis of pulmonary TB by modelling human genetic diversity with the Diversity Outbred mouse population. When infected with M. tuberculosis, one-third develop early onset, rapidly progressive, necrotizing granulomas and succumb within 60 days. The remaining develop non-necrotizing granulomas and survive longer than 60 days. Genetic mapping using clinical indicators of disease, granuloma histopathological features, and immune response traits identified five new loci on mouse chromosomes 1, 2, 4, 16 and three previously identified loci on chromosomes 3 and 17. Quantitative trait loci (QTLs) on chromosomes 1, 16, and 17, associated with multiple correlated traits and had similar patterns of allele effects, suggesting these QTLs contain important genetic regulators of responses to M. tuberculosis. To narrow the list of candidate genes in QTLs, we used a machine learning strategy that integrated gene expression signatures from lungs of M. tuberculosis-infected Diversity Outbred mice with gene interaction networks, generating functional scores. The scores were then used to rank candidates for each mapped trait in each locus, resulting in 11 candidates: Ncf2, Fam20b, S100a8, S100a9, Itgb5, Fstl1, Zbtb20, Ddr1, Ier3, Vegfa, and Zfp318. Importantly, all 11 candidates have roles in infection, inflammation, cell migration, extracellular matrix remodeling, or intracellular signaling. Further, all candidates contain single nucleotide polymorphisms (SNPs), and some but not all SNPs were predicted to have deleterious consequences on protein functions. Multiple methods were used for validation including (i) a statistical method that showed Diversity Outbred mice carrying PWH/PhJ alleles on chromosome 17 QTL have shorter survival; (ii) quantification of S100A8 protein levels, confirming predicted allele effects; and (iii) infection of C57BL/6 mice deficient for the S100a8 gene. Overall, this work demonstrates that systems genetics using Diversity Outbred mice can identify new (and known) QTLs and new functionally relevant gene candidates that may be major regulators of granuloma necrosis and acute inflammation in pulmonary TB.

Seasonal Flight Patterns of Chrysodeixis includens (Lepidoptera: Noctuidae) in the Florida Panhandle and Inventory of Plusiine Species Cross-Attracted to Synthetic Pheromone.

Chysodeixis includens (Walker) is a polyphagous economic pest in agricultural landscapes. To detect the occurrence of this pest in the field, trapping using sex pheromone lures is often implemented. However, other plusiine species are cross-attracted to these lures and may be misidentified as C. includens due to their morphological similarities. The objectives of this study were to provide region-specific information on the abundance of C. includens throughout the year as well as document the occurrence of related plusiines cross-attracted to C. includens sex pheromone traps in the Florida Panhandle. Twelve commercial fields of peanut and twelve commercial fields of cotton located across Escambia, Santa Rosa, and Jackson counties were monitored with Trécé delta traps baited with C. includens sex pheromone lures (Alpha Scents, Inc.; West Linn, OR) from June 2017 to June 2019. There was no difference in C. includens flight across dryland or irrigated fields. Identifications revealed that in addition to C. includens, the following species of the subfamily Plusiinae were crossed-attracted: Argyrogramma verruca (Fabricius) (Lepidoptera: Noctuidae), Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae), Ctenoplusia oxygramma (Geyer) (Lepidoptera: Noctuidae), and Rachiplusia ou (Guenée) (Lepidoptera: Noctuidae). The occurrence of each species in the region and their flight phenology are documented. Chysodeixis includens abundance was greatest in September and decreased through December. Due to the high abundance of C. oxygramma and similar flight phenology to C. includens, this is the likeliest species to skew estimations and influence management decisions of C. includens, especially early in the crop season, when C. includens abundance is low.

Monitoring of uncaging processes by designing photolytical reactions.

The use of photolabile protecting groups (PPGs) has been growing in emphasis for decades, and nowadays they enable cutting-edge results in numerous fields ranging from organic synthesis to neurosciences. PPGs are chemical entities that can be conjugated to a biomolecule to hide its biological activity, forming a stable so called "caged compound". This conjugate can be simply cleaved by light and therefore, the functionality of the biomolecule is restored with the formation of a PPG by-product. However, there is a sizeable need for PPGs that are able to quantify the "uncaging" process. In this review, we will discuss several strategies leading to an acute quantification of the uncaging events by fluorescence. In particular, we will focus on how molecular engineering of PPG could open new opportunities by providing easy access to photoactivation protocols.

Biotic potential and reproductive parameters of Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) in the laboratory / Potencial biótico e parâmetros reprodutivos de Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) em laboratório

Abstract In this study we evaluate the biotic potential and reproductive parameters of Spodoptera cosmioides (Walker, 1858) under controlled conditions (25 ± 1 °C, 70 ± 10% RH and 14 hour photophase). Females, on average, (14.433 days) did not live significantly longer than their male counterparts (13.100 days). The mean durations of the pre-oviposition, oviposition and post-oviposition periods were 2.767, 10.600 and 1.067 days, respectively. The mean fecundity of females was 5,073.533 eggs and the mean fertility was 5,021.027 larvae. On average, females copulated 1.167 times. Fecundity was positively correlated with the number of copulations (r = 0.583, P <0.001). The number of copulations, however, was negatively correlated whit the duration of the pre-oviposition (r = -0.560, P = 0.007) and oviposition (r = -0.479, P = 0.048) periods, and overall longevity (r = -0.512, P = 0.031). The biotic potential was estimated at 6.547 x 1021 individuals/female/year. The net reproductive rate (Ro) was 2,193.722 times per generation and the mean generation time (T) was 46.407 days. The intrinsic rate of increase (rm) was 0.166, with a finite rate of increase (λ) of 1.180, per week.

Resumo Neste estudo avaliamos o potencial biótico e parâmetros reprodutivos de Spodoptera cosmioides (Walker, 1858) em condições controladas (25 ± 1 °C, 70 ± 10% UR e 14 horas de fotofase). Em média, as fêmeas (14,433 dias) não viveram significativamente mais do que os machos (13,100 dias). A duração média dos períodos de pre-oviposição, oviposição e pós-oviposição foram 2,767; 10,600 e 1,067 dias, respectivamente. A fecundidade média das fêmeas foi de 5.073,533 ovos e a fertilidade média foi de 5.021,027 larvas. Em média, as fêmeas copularam 1,167 vezes. A fecundidade correlacionou-se positivamente com o número de cópulas (r = 0,583; P <0,001). Entretanto, o número de cópulas correlacionou-se negativamente com a duração dos períodos de pré-oviposição (r = -0,560; P = 0,007), oviposição (r = -0,479; P = 0;048) e a longevidade (r = -0,512; P = 0,031). O potencial biótico foi estimado em 6,547 x 1021 indivíduos/fêmea/ano. A taxa líquida de reprodução (Ro) foi de 2.193,722 vezes por geração e o tempo médio de cada geração (T) foi de 46,407 dias. A taxa intrínseca de crescimento (rm) foi 0,166, com uma taxa finita de aumento (λ) de 1,180, por semana.

The External Morphology of Mythimna (Pseudaletia) sequax (Lepidoptera: Noctuidae).

Mythimna (Pseudaletia) sequax Franclemont, 1951 (Lepidoptera: Noctuidade) is the most important armyworm in the American tropics and subtropics. In this study, we describe the external morphology of the immatures and adults, based on larvae fed on quicuio grass (Pennisetum clandestinum Hochst. ex Chiov - Poaceae). The external morphology of the eggs, larvae, pupae, and adults is described and illustrated. Important taxonomic structures of the larvae are described, including hypopharynx, spinneret, and mandible. Adult structures that allow the differentiation of the species, such as male genitalia and scent brushes, are also described. The results are compared and discussed based on morph functionality and with publications on the congeneric species.

Owlet moths (Lepidoptera: Noctuoidea) associated with Bt and non- Bt soybean in the brazilian savanna / Noctuóides (Lepidoptera: Noctuoidea) associados a soja Bt e não-Bt no Cerrado brasileiro

Abstract The use of GMO expressing Bt toxin in soybean production has increased significantly in the last years in Brazil in order to manage the damage caused by lepidopteran pests. In this study, we compared the richness and abundance of owlet moths (Noctuoidea) associated with Bt and non-Bt soybean. We determined the temporal variations as a function of phenology, and correlated the population variations of the most common species with meteorological variables. The research was conducted at the experimental area of Embrapa Cerrados. The collection method used was differentiated being suppressive and absolute. A total of 13 species were collected, of which eight occurred on Bt soybeans. The most representative taxa were Chrysodeixis includens (72.87%), Anticarsia gemmatalis (18.17%) and Spodoptera spp (5.22%). The number of larvae belonging to species targeted by the Bt technology was 10 times lower on Bt than on non-Bt soybeans. Utetheisa ornatrix and Elaphria deltoides were recorded on soybean for the first time, observing larvae of both species in non-Bt soybean and those of U. ornatrix also in Bt soybean. Only A. gemmatalis larvae correlated (p <0.05) negatively with precipitation. This study provided field information on the abundance and species richness of owlet moths on non-Bt soybeans, associated with the effects of Bt soybean. When considering the different levels of infestation between cultivars as a criterion, larvae monitoring is of substantial importance in order to develop the lost control program.

Resumo O uso de OGM que expressam toxina Bt na produção de soja tem aumentado significativamente nos últimos anos no Brasil e são utilizados para conter os danos causados ​​pelos lepidópteros pragas. Neste estudo comparamos a riqueza e a abundância de Noctuoides (Noctuoidea) associados à soja Bt e não-Bt. Determinamos as variações temporais em função da fenologia e correlacionamos às variações populacionais das espécies mais comuns com variáveis ​​meteorológicas. A pesquisa foi conduzida na área experimental da Embrapa Cerrados. O método de coleta utilizado foi diferenciado sendo supressivo e absoluto. Um total de 13 espécies foram coletadas, das quais oito ocorreram em soja Bt. Os taxa mais representativos foram Chrysodeixis includens, Anticarsia gemmatalis e Spodoptera spp. O número de larvas pertencentes às espécies alvo da tecnologia Bt foram 10 vezes menores na soja Bt do que em soja não-Bt . Utetheisa ornatrix e Elaphria deltoides foram registradas na soja pela primeira vez, observando-se larvas de ambas espécies na soja não-Bt e as de U. ornatrix também na soja Bt. Somente as larvas de A. gemmatalis se correlacionaram (p <0,05) negativamente com a precipitação. Este estudo forneceu informações em campo sobre a abundância e riqueza de espécies na soja não- Bt, associada aos efeitos da soja Bt. A importância do monitoramento das lagartas é substancial, a fim de tomar a melhor decisão de controle, considerando-se os diferentes níveis de infestação entre cultivares como critério.

Overlooked South American noctuid species: revalidation of Feltia llanoi stat. rev. and redescription of Feltia brachystria (Lepidoptera: Noctuidae).

Feltia llanoi (Köhler, 1953) stat. rev. and its hitherto senior subjective synonym Feltia brachystria (Hampson, 1903) are two species of noctuid moths with unusual broadly bipectinate antenna and restricted distribution in central eastern Argentina, southern Brazil, and Uruguay. Examination of the type of specimens and further material from several collections indicates that these names are not synonyms, but valid species, and reveal the occurrence of F. llanoistat. rev. in Brazil. Therefore, F. llanoistat. rev. and F. brachystria are redescribed and the former name is reinstated to species, including taxonomic comments, illustration of some characters of taxonomic importance, and of structures of the male and female genitalia. The species are compared with similar-looking and supposedly closely related species, such as F. chilensis (Hampson, 1903), F. carolia (Schaus, 1929), and F. gypaetina (Guenée, 1852). Additionally, in order to stabilize nomenclature, a lectotype for F. llanoistat. rev. is designated, and Agrotis daguerrei Köhler, 1961 is here recognized as a junior subjective synonym of F. llanoistat. rev. (syn. nov.).

Feltia submontana (Noctuidae, Noctuinae): Redescription, Taxonomy, Life Cycle, and Spatial Distribution of a Neglected South American Potential Pest Species.

Feltia submontana (Köhler, 1961) is redescribed based on specimens from Northwestern Argentina and Central and Southeastern Brazil. Taxonomic comments, photographs of the adults, characters of taxonomic importance, and illustrations of structures of the labial palpus, legs, and male and female genitalia are provided. The species is compared with similar-looking and supposedly closely related species, such as F. hispidula (Guenée, 1852) and F. lilacina (Zerny, 1916). The species, originally described for Argentina, is reported for Brazil for the first time. Most Brazilian specimens come from the "Cerrado" but also from Southeastern Atlantic Forests. The life cycle of F. submontana specimens collected in Planaltina, Distrito Federal, Brazil, is described; the species probably has only a single generation per year and imagines are on the wing in the late autumn and early winter months; the last instar prepupa and pupa pass through aestival diapause. The abundance of F. submontana relative to other species of Agrotis Ochsenheimer, 1816, and Feltia Walker, 1856, in the above-cited locality is accessed through 4 years of standardized collecting with light trap; the species is the second most abundant species of these genera in the area, with about one fifth of the captures, second only to A. ipsilon (Hufnagel, 1766), with about two thirds of the captures, and about two times more abundant than F. subterranea (Fabricius, 1794); the latter two are regarded as important pest species in South America.

Owlet moths (Lepidoptera: Noctuoidea) associated with Bt and non- Bt soybean in the brazilian savanna.

The use of GMO expressing Bt toxin in soybean production has increased significantly in the last years in Brazil in order to manage the damage caused by lepidopteran pests. In this study, we compared the richness and abundance of owlet moths (Noctuoidea) associated with Bt and non-Bt soybean. We determined the temporal variations as a function of phenology, and correlated the population variations of the most common species with meteorological variables. The research was conducted at the experimental area of Embrapa Cerrados. The collection method used was differentiated being suppressive and absolute. A total of 13 species were collected, of which eight occurred on Bt soybeans. The most representative taxa were Chrysodeixis includens (72.87%), Anticarsia gemmatalis (18.17%) and Spodoptera spp (5.22%). The number of larvae belonging to species targeted by the Bt technology was 10 times lower on Bt than on non-Bt soybeans. Utetheisa ornatrix and Elaphria deltoides were recorded on soybean for the first time, observing larvae of both species in non-Bt soybean and those of U. ornatrix also in Bt soybean. Only A. gemmatalis larvae correlated (p <0.05) negatively with precipitation. This study provided field information on the abundance and species richness of owlet moths on non-Bt soybeans, associated with the effects of Bt soybean. When considering the different levels of infestation between cultivars as a criterion, larvae monitoring is of substantial importance in order to develop the lost control program.

Where and when? How phenological patterns of armyworm moths (Lepidoptera: Noctuidae) change along a latitudinal gradient in Brazil.

The phenological patterns exhibited by different organisms are known as adaptive responses to the cyclical environmental conditions. However, only a limited number of researches explore which factors are responsible for these phenological patterns in pest species. In the current study, abundance patterns were studied in the phenology of three Spodoptera Guenée, 1852 species, along the 29° latitudinal gradient in South America. The goal was to test whether widely distributed and abundant crop pest species would exhibit different phenological responses to seasonal meteorological variables and host plant availability. To test this, 13 light traps were set up in Brazil to collect adult Spodoptera samples at the time of the new moon, every month, from June 2015 to May 2016. The time of occurrence and intensity of the phenology were determined for each species, employing circular statistics. Both metrics revealed significant variations among the different species, as well as the factors associated with them. Latitude was found to affect the period of occurrence in Spodoptera cosmioides (Walker, 1858) and Spodoptera albula (Walker, 1857), whereas in Spodoptera frugiperda (J. E. Smith, 1797) its effect was evident only in the intensity of its phenology. Further, both meteorological variables and host plant availability in the sampling sites produced predictive models to account for the phenological patterns expressed. These findings suggest that different species of Spodoptera exhibit different adaptive strategies in their life cycles in response to environmental conditions, thus necessitating specific management practices regarding their seasonal population fluctuation.

Spodoptera marima: a New Synonym of Spodoptera ornithogalli (Lepidoptera: Noctuidae), with Notes on Adult Morphology, Host Plant Use and Genetic Variation Along Its Geographic Range.

Spodoptera marima (Schaus, 1904) and Spodoptera ornithogalli (Guenée, 1852) are noctuid moths recognized for their economic importance in the New World. Historically, they have been considered cryptic species, being supposedly differentiated from each other by the absence of sexual dimorphism in S. marima, subtle differences in coloration on the hindwings and scale tufts of the ovipositor and the shape of male valva. The species are allopatric: North and Central America for S. ornithogalli and South America for S. marima. Previous phylogenetic studies using multilocus DNA sequences including a few specimens of S. marima suggested these species merge into a single clade. Here, we performed a comparative analysis to elucidate further their taxonomic status, using specimens of S. marima from various localities in Brazil. These species were recovered in the phylogenetic analysis as consistently mixed with each other in a major well-supported clade within Spodoptera. The existence of a spatial pattern for genetic structure was not clearly supported when two biogeographic regions (Neotropics and Neartic) were taken in separation; however, an individual-level isolation by distance was significant. The morphological characters traditionally used to differentiate the species were also not consistent. Therefore, we now consider S. marima a new junior synonym of S. ornithogalli. Results on host plant use demonstrated that it is a polyphagous species using more than 200 plants, which may mediate phenotypic variation along its geographical range.

Biotic potential and reproductive parameters of Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) in the laboratory.

In this study we evaluate the biotic potential and reproductive parameters of Spodoptera cosmioides (Walker, 1858) under controlled conditions (25 ± 1 °C, 70 ± 10% RH and 14 hour photophase). Females, on average, (14.433 days) did not live significantly longer than their male counterparts (13.100 days). The mean durations of the pre-oviposition, oviposition and post-oviposition periods were 2.767, 10.600 and 1.067 days, respectively. The mean fecundity of females was 5,073.533 eggs and the mean fertility was 5,021.027 larvae. On average, females copulated 1.167 times. Fecundity was positively correlated with the number of copulations (r = 0.583, P <0.001). The number of copulations, however, was negatively correlated whit the duration of the pre-oviposition (r = -0.560, P = 0.007) and oviposition (r = -0.479, P = 0.048) periods, and overall longevity (r = -0.512, P = 0.031). The biotic potential was estimated at 6.547 x 1021 individuals/female/year. The net reproductive rate (Ro) was 2,193.722 times per generation and the mean generation time (T) was 46.407 days. The intrinsic rate of increase (rm) was 0.166, with a finite rate of increase (λ) of 1.180, per week.

o-Nitrobenzyl photoremovable groups with fluorescence uncaging reporting properties.

o-Nitrobenzyl (o-NB) derivatives are the most widely applied photoremovable groups for the study of dynamic biological processes. By introducing different substituents to the benzylic position we were able to generate a fluorescence signal upon irradiation. This signal originates from the formation of a nitrosoketone by-product able to achieve a keto-enol tautomerism leading to pi-conjugated α-hydroxystilbene derivatives. These o-NB caging groups can be used to directly monitor the uncaging event by the release of a detectable fluorescent side-product.

Morphological Characterization of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae: Heliothinae).

The cotton bollworm Helicoverpa armigera (Hübner) is a widespread lepidopteran pest found in various crops worldwide. This highly polyphagous species, commonly found both in the Old and New World, has caused significant economic damage as an invasive agricultural pest in Brazil since 2013. The goal of the present study is to provide a detailed morphological assessment of adults and immature stages of H. armigera, as this species is often confused with H. zea (Boddie), a congeneric species that is native to the New World. The biology data were acquired during four full life cycles, and observations on general behavior, nocturnal habits of larvae and adults, and sensitivity of larvae to humidity were recorded. Larval chaetotaxy differs between the first and the remaining instars, which bear L2 on the meso- and metathorax and L3 on A3 through A6, along with conspicuous chalazae and longitudinal bands. Important morphological characters of this species include the following: eggs with four micropylar openings, lined with 12 cells arranged in the shape of a rosette; pupa adecticous and obtect, with prominent spiracles; adults with the distal antennomere striate. Adults exhibit sexual dimorphism in the number of setae on the frenulum and spines on the prothoracic leg. Illustrations of the critical morphological features of this species are provided.

Biotic Potential and Life Table of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from Three Brazilian Regions.

This study aimed to evaluate the biotic potential and life table of individuals of Helicoverpa armigera (Hübner) from different host plants (citrus, corn, and cotton) and Brazilian states (São Paulo, Distrito Federal, and Bahia) in artificial diet, under laboratory conditions (25 ± 1°C, 70 ± 10% RH, 14 h photophase). The longevity, pre-, post- and oviposition periods, fecundity, and fertility of 15 mating pairs per origin were evaluated. We also compared the reproductive parameters of each group of insects (São Paulo (SP), Distrito Federal (DF), and Bahia (BA)), including the net reproductive rate (Ro), mean generation time (T), intrinsic rate of increase (r m), and finite rate of increase (λ). Microsatellite analysis from individuals collected in different locations and host plants did not show differences among the parental insects. It was verified that parental progeny collected in cotton fields from Bahia had a higher biotic potential, a higher reproductive rate (Ro), and a better fecundity compared to the insects from remaining regions. The life table charts indicate that the highest values for the reproductive parameters of the Bahia progeny are associated with higher specific fertility, particularly in early adulthood. The greatest biotic potential of the Bahia progeny may be due to increased selection pressure from the insecticide used (organophosphate and pyrethroid) on cotton crops compared to that of other crops, as well due to the massive adoption of Bt cotton-producing areas of that state from 2013 outbreaks.

Owlet moths (Lepidoptera: Noctuoidea) associated with Bt and non- Bt soybean in the brazilian savanna

Abstract The use of GMO expressing Bt toxin in soybean production has increased significantly in the last years in Brazil in order to manage the damage caused by lepidopteran pests. In this study, we compared the richness and abundance of owlet moths (Noctuoidea) associated with Bt and non-Bt soybean. We determined the temporal variations as a function of phenology, and correlated the population variations of the most common species with meteorological variables. The research was conducted at the experimental area of Embrapa Cerrados. The collection method used was differentiated being suppressive and absolute. A total of 13 species were collected, of which eight occurred on Bt soybeans. The most representative taxa were Chrysodeixis includens (72.87%), Anticarsia gemmatalis (18.17%) and Spodoptera spp (5.22%). The number of larvae belonging to species targeted by the Bt technology was 10 times lower on Bt than on non-Bt soybeans. Utetheisa ornatrix and Elaphria deltoides were recorded on soybean for the first time, observing larvae of both species in non-Bt soybean and those of U. ornatrix also in Bt soybean. Only A. gemmatalis larvae correlated (p 0.05) negatively with precipitation. This study provided field information on the abundance and species richness of owlet moths on non-Bt soybeans, associated with the effects of Bt soybean. When considering the different levels of infestation between cultivars as a criterion, larvae monitoring is of substantial importance in order to develop the lost control program.

Resumo O uso de OGM que expressam toxina Bt na produção de soja tem aumentado significativamente nos últimos anos no Brasil e são utilizados para conter os danos causados pelos lepidópteros pragas. Neste estudo comparamos a riqueza e a abundância de Noctuoides (Noctuoidea) associados à soja Bt e não-Bt. Determinamos as variações temporais em função da fenologia e correlacionamos às variações populacionais das espécies mais comuns com variáveis meteorológicas. A pesquisa foi conduzida na área experimental da Embrapa Cerrados. O método de coleta utilizado foi diferenciado sendo supressivo e absoluto. Um total de 13 espécies foram coletadas, das quais oito ocorreram em soja Bt. Os taxa mais representativos foram Chrysodeixis includens, Anticarsia gemmatalis e Spodoptera spp. O número de larvas pertencentes às espécies alvo da tecnologia Bt foram 10 vezes menores na soja Bt do que em soja não-Bt . Utetheisa ornatrix e Elaphria deltoides foram registradas na soja pela primeira vez, observando-se larvas de ambas espécies na soja não-Bt e as de U. ornatrix também na soja Bt. Somente as larvas de A. gemmatalis se correlacionaram (p 0,05) negativamente com a precipitação. Este estudo forneceu informações em campo sobre a abundância e riqueza de espécies na soja não- Bt, associada aos efeitos da soja Bt. A importância do monitoramento das lagartas é substancial, a fim de tomar a melhor decisão de controle, considerando-se os diferentes níveis de infestação entre cultivares como critério.

Biotic potential and reproductive parameters of Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) in the laboratory

Abstract In this study we evaluate the biotic potential and reproductive parameters of Spodoptera cosmioides (Walker, 1858) under controlled conditions (25 ± 1 °C, 70 ± 10% RH and 14 hour photophase). Females, on average, (14.433 days) did not live significantly longer than their male counterparts (13.100 days). The mean durations of the pre-oviposition, oviposition and post-oviposition periods were 2.767, 10.600 and 1.067 days, respectively. The mean fecundity of females was 5,073.533 eggs and the mean fertility was 5,021.027 larvae. On average, females copulated 1.167 times. Fecundity was positively correlated with the number of copulations (r = 0.583, P 0.001). The number of copulations, however, was negatively correlated whit the duration of the pre-oviposition (r = -0.560, P = 0.007) and oviposition (r = -0.479, P = 0.048) periods, and overall longevity (r = -0.512, P = 0.031). The biotic potential was estimated at 6.547 x 1021 individuals/female/year. The net reproductive rate (Ro) was 2,193.722 times per generation and the mean generation time (T) was 46.407 days. The intrinsic rate of increase (rm) was 0.166, with a finite rate of increase () of 1.180, per week.

Resumo Neste estudo avaliamos o potencial biótico e parâmetros reprodutivos de Spodoptera cosmioides (Walker, 1858) em condições controladas (25 ± 1 °C, 70 ± 10% UR e 14 horas de fotofase). Em média, as fêmeas (14,433 dias) não viveram significativamente mais do que os machos (13,100 dias). A duração média dos períodos de pre-oviposição, oviposição e pós-oviposição foram 2,767; 10,600 e 1,067 dias, respectivamente. A fecundidade média das fêmeas foi de 5.073,533 ovos e a fertilidade média foi de 5.021,027 larvas. Em média, as fêmeas copularam 1,167 vezes. A fecundidade correlacionou-se positivamente com o número de cópulas (r = 0,583; P 0,001). Entretanto, o número de cópulas correlacionou-se negativamente com a duração dos períodos de pré-oviposição (r = -0,560; P = 0,007), oviposição (r = -0,479; P = 0;048) e a longevidade (r = -0,512; P = 0,031). O potencial biótico foi estimado em 6,547 x 1021 indivíduos/fêmea/ano. A taxa líquida de reprodução (Ro) foi de 2.193,722 vezes por geração e o tempo médio de cada geração (T) foi de 46,407 dias. A taxa intrínseca de crescimento (rm) foi 0,166, com uma taxa finita de aumento () de 1,180, por semana.

Reproductive potential of Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae) in the laboratory: effect of multiple couples and the size / Potencial reprodutivo de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae) em laboratório: efeito de múltiplos casais e do tamanho

Abstract This study aimed to evaluate the effect of keeping three couples in the same cage, and the size of adults emerged from small, medium-sized and large pupae (278.67 mg; 333.20 mg and 381.58 mg, respectively), on the reproductive potential of S. eridania (Stoll, 1782) adults, under controlled conditions (25 ± 1 °C, 70% RH and 14 hour photophase). We evaluated the survival, number of copulations, fecundity and fertility of the adult females. The survival of females from these different pupal sizes did not differ statistically, but the survival of males from large pupae was statistically shorter than from small pupae. Fecundity differed significantly and correlated positively with size. The number of effective copulations (espematophores) and fertility did not vary significantly with pupal size. Our results emphasize the importance of indicating the number of copulations and the size of the insects when reproductive parameters are compared.

Resumo Este estudo objetivou avaliar o efeito de confinar três casais em cada gaiola e o tamanho de adultos emergidos de pupas pequenas, medias e grandes (278,67 mg, 333,20 mg e 381,58 mg, respectivamente), sobre o potencial reprodutivo de S. eridania (Stoll, 1782), em condições controladas (25 ± 1 °C, 70% UR e 14 horas de fotofase). Avaliou-se a sobrevivência, o número de cópulas, fecundidade e fertilidade dos adultos. A sobrevivência não diferiu significativamente entre fêmeas provenientes de pupas de diferentes tamanhos, mas os machos oriundos de pupas grandes tiveram sobrevivência significativamente menor que os demais tamanhos. A fecundidade diferiu significativamente e correlacionou-se positivamente com o tamanho. O número de cópulas (espematóforos) e a fertilidade não variaram em função do peso pupal. Os resultados enfatizam a importância de indicar o número de cópulas e o tamanho dos insetos estudados para que comparações entre os parâmetros reprodutivos possam ser efetuadas.

Reproductive potential of Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae) in the laboratory: effect of multiple couples and the size.

This study aimed to evaluate the effect of keeping three couples in the same cage, and the size of adults emerged from small, medium-sized and large pupae (278.67 mg; 333.20 mg and 381.58 mg, respectively), on the reproductive potential of S. eridania (Stoll, 1782) adults, under controlled conditions (25 ± 1 °C, 70% RH and 14 hour photophase). We evaluated the survival, number of copulations, fecundity and fertility of the adult females. The survival of females from these different pupal sizes did not differ statistically, but the survival of males from large pupae was statistically shorter than from small pupae. Fecundity differed significantly and correlated positively with size. The number of effective copulations (espematophores) and fertility did not vary significantly with pupal size. Our results emphasize the importance of indicating the number of copulations and the size of the insects when reproductive parameters are compared.

Lasiothyris luminosa (Razowski & Becker) (Lepidoptera: Tortricidae): a New Grapevine Pest in Northeastern Brazil.

The little-known Neotropical tortricid moth Lasiothyris luminosa (Razowski & Becker) is reported for the first time as a grapevine pest in northeastern Brazil. A diagnosis based on morphological characters including the genitalia is provided for both sexes, together with a preliminary description of economic damage caused by the larval stage on flowers and fruits. In addition, we sequenced a partial region of the cytochrome oxidase subunit I gene from specimens from the region and reconstructed phylogenetic relationships using representative lineages of closely related tortricids as terminals.