Long-term fertilization and manuring effects on the nexus between sulphur distribution and SOC in an Inceptisol over five decades under a finger millet-maize cropping system.

Our investigation revealed that alterations in sulphur (S) pools are predominantly governed by soil organic carbon (SOC), soil nitrogen (N), microbial biomass, and soil enzyme activities in sandy clay loam (Vertic Ustropept) soil. We employed ten sets of nutrient management techniques, ranging from suboptimal (50% RDF) to super-optimal doses (150% RDF), including NPK + Zn, NP, N alone, S-free NPK fertilizers, NPK + FYM, and control treatments, to examine the interrelation of S with SOC characteristics. Fourier-transform infrared (FT-IR) spectroscopy was utilized to analyze the functional groups present in SOC characterization across four treatments: 100% NPK, 150% NPK, NPK + FYM, and absolute control plots. Principal component analysis (PCA) was then applied to assess 29 minimal datasets, aiming to pinpoint specific soil characteristics influencing S transformation. In an Inceptisol, the application of fertilizers (100% RDF) in conjunction with 10 t ha-1 of FYM resulted in an increase of S pools from the surface to the subsurface stratum (OS > HSS > SO42--S > WSS), along with an increase in soil N and SOC. FT-IR spectroscopy identified cellulose and thiocyanate functional groups in all four plots, with a pronounced presence of carbohydrate-protein polyphenol, sulfoxide (S=O), and nitrate groups specifically observed in the INM plot. The PCA findings indicated that the primary factors influencing soil quality and crop productivity (r2 of 0.69) are SOC, SMBC, SMBN, SMBS, and the enzyme activity of URE, DHA, and AS. According to the study, the combined application of fertilizer and FYM (10 t ha-1) together exert a positive impact on sulphur transformation, SOC accumulation, and maize yield in sandy clay loam soil.

Recent Surge in Mumps Cases in India: Need for Urgent Remedial Measures.

Mumps is a global public health problem caused by mumps virus, a member of paramyxoviridae family. MMR (Mumps, Measles, Rubella), an effective vaccine, has been incorporated into routine immunization schedules in over 100 countries. On the contrary, in India, vaccine against mumps has not been included in the routine immunization schedule as mumps is still not viewed as a significant public health problem by the government to warrant such an intervention. An increasing number of mumps outbreaks being reported from many parts of the country in the recent past, is matter of concern. The current paper reviews the situation of mumps in India including the recent surge, and discusses the remedial measures to contain these outbreaks. We conclude that inclusion of Mumps component as MMR vaccine in the Universal Immunization Programme of India along with strengthening surveillance is required to tackle the situation.

Is telemedicine a holy grail in healthcare policy: clinicians' and patients' perspectives from an Apex Institution in Western India.

BACKGROUND: This study aimed to explore the perception of doctors regarding telemedicine consultations and the level of patient satisfaction with the services received through teleconsultations. METHODS: This cross-sectional study was conducted on clinicians who provided teleconsultations and patients who received teleconsultations in an Apex healthcare institution in Western India. Semi-structured interview schedules were used to record the quantitative and qualitative information. Clinicians' perceptions and patients' satisfaction were assessed using two different 5-point Likert scales. Data were analyzed using SPSS v.23 using non-parametric tests (Kruskal Wallis and Mann-Whitney U). RESULTS: A total of 52 clinicians who delivered teleconsultations and 134 patients who received teleconsultations from those doctors were interviewed in this study. For 69% of doctors, telemedicine was feasible to implement, and for the rest, it was challenging. Doctors believe telemedicine is convenient for patients (77%) and prevents the transmission of infection (94.2%). Difficulty in clinical evaluation (73%), communication (55.7%), network connectivity (34%), diagnosis and investigations (32%), and patients' e-illiteracy (32%) were the most common challenges faced by clinicians. Patients' experiences were positive in terms of ease of registration (82.1%), audio quality (100%), freedom to discuss medicine (94.8%), and comprehension of the diagnoses (88.1%). Patients expressed satisfaction with the length of the teleconsultation (81.4%), the advice and care they received (78.4%), and the manner and communication of the clinicians (78.4%). CONCLUSIONS: Though there were some challenges in the implementation of telemedicine, the clinicians perceived it quite helpful. The majority of the patients were satisfied with teleconsultation services. Difficulty in registration, lack of communication, and a deep-rooted mindset of physical consultations were the primary concerns from the patient side.

Effect of Citrullus colocynth fruit extract on pro and anti apoptotic molecules in human melanoma cell line (A375).

Melanoma remains a major cause of death worldwide. Chemotherapy might be used to treat advanced Melanoma which is associated with some side effects. Hence, providing proper treatment or proper employment of lower toxic substances is essential. Citrullus colocynthis, a medicinal plant, seems a potential anticancer herbal medicine against Melanoma via various efficient compounds. Therefore, it is of interest to analyze the effect of Citrullus colocynthis fruit extract on pro and anti apoptotic molecules in the human melanoma cell line (A375). Cell viability test was done using MTT assay. mRNA expression of Bax and Bcl2 was done by real-time PCR. The data was analyzed statistically by one way analysis of variance and Duncan multiple range test with graph prism version 5. p<0.05 was considered significant. Citrullus colocynthis extract caused a marked increase in cell death in a dose-dependent manner. At the end of 48 hours, maximum inhibition (50%) was at 400 and 500µg/ml. The study demonstrated that the effect of Citrullus colocynthis extract has elevated the Bax mRNA expression on human melanoma cell lines. Data shows that Citrullus colocynthis fruit extract has an anticancer effect on the human melanoma cell line (A375) by increasing the expression of Bax and Bcl2 and this could be because of the phytochemicals present in it.

Evaluation of respiratory function among homemakers exposed to indoor air pollution.

Indoor air contamination is a condition that can increase cardiovascular and respiratory mortality in the present-day population. Utilizing cooking exhaust in working environments, pollutants such as Polycyclic aromatic hydrocarbons (PAHs), incense sticks, cooking in fuel and kindling oven in the kitchen, and tobacco smoke are the significant reasons for indoor air contamination. The point of this study is to make mindfulness among homemakers presented to indoor air contamination. A spellbinding cross-sectional review including 100 homemaker female subjects in the age of 40-50 know about indoor air contamination. An overview was directed among 100 home creators in regard to indoor air contamination to make mindfulness. The strategy for testing done is basic arbitrary inspecting. The aftereffects of the current review showed that respondents were greatly presented to the indoor air contamination and created side effects connected with it and generally respondents in the age gathering of 40-50 years had encountered more exhaustion, dry bothersome skin, copying and aggravation of the eyes and stodgy nose, and affiliation was measurably huge. This finding revealed the indoor air toxins that can be considered to assume a part underway to infections like asthma, and so forth.

An in vitro analysis on the antioxidant and anti-diabetic properties of Kaempferia galanga rhizome using different solvent systems.

Kaempferia galanga rhizome (KGR) is a stemless, sweet-smelling, enduring, and rhizomatous monocotyledonous plant of the ginger family also called sand ginger or kencur. lt possesses many pharmacological effects and the goal of this study is to use several solvent solutions to determine the antioxidant and anti-diabetic potential of KGR. By observing KGR's alpha-glucosidase and alpha-amylase inhibitory activity, the in vitro diabetic activity was assessed. The in vitro cancer preventive action was evaluated by doing the 2,2-diphenyl-1-picrylhydrazyl test. The outcomes of the study showed increase in dose-dependent percentage of inhibition in α-amylase and α-glucosidase activity for both extract and standard ranging from 100 to 500 µg/ml. The current review proposes that the concentrate of KGR could be utilized for the treatment of diabetes mellitus as well with respect to dealing with the oxidative stress-related diseases.

N-Containing Carbon/Graphene Nanocomposites for Electrochemical Supercapacitor Applications.

Hetero atoms containing conductive nanocarbon materials are being studied extensively for their electrochemical energy storage and conversion applications. Herein, we report a facile process for the preparation of N-containing carbon/graphene nanocomposites by simultaneous thermal decomposition of polypyrrole into N-containing carbon and reduction of graphene oxide into graphene in H2/Ar atmosphere. The XRD pattern of N-containing carbon/graphene nanocomposites prepared at different temperatures indicated the formation of reduced graphene oxide from the reduction of GO. The FT-IR and Raman spectroscopic analysis revealed the presence of N atoms in the nanocomposites and the elemental analysis was used to estimate the amount of N in the nanocomposite. The XPS analysis distinguished the pyridine, pyrrolic and quaternary forms of N present in the nanocomposite. The slow decomposition of polypyrrole resulted in the mesoporous structure to the resulting nanocomposite, which was confirmed by the BET adsorption­desorption isotherm. The electron microscopic analysis confirmed the presence of highly transparent carbon nanosheets. The amount of N in the nanocomposite that depends on the decomposition temperature was found to influence the electrochemical performance. The nanocomposite prepared at 700 °C showed a large specific capacitance of 296 F/g with an excellent cycling stability of 93% after 1000 cycles.

Neutralization of cobra venom by cocktail antiserum against venom proteins of cobra (Naja naja naja).

Naja naja venom was characterized by its immunochemical properties and electrophoretic pattern which revealed eight protein bands (14 kDa, 24 kDa, 29 kDa, 45 kDa, 48 kDa, 65 kDa, 72 kDa and 99 kDa) by SDS-PAGE in reducing condition after staining with Coomassie Brilliant Blue. The results showed that Naja venom presented high lethal activity. Whole venom antiserum or individual venom protein antiserum (14 kDa, 29 kDa, 65 kDa, 72 kDa and 99 kDa) of venom could recognize N. naja venom by Western blotting and ELISA, and N. naja venom presented antibody titer when assayed by ELISA. The neutralization tests showed that the polyvalent antiserum neutralized lethal activities by both in vivo and in vitro studies using mice and Vero cells. The antiserum could neutralize the lethal activities in in-vivo and antivenom administered after injection of cobra venom through intraperitoneal route in mice. The cocktail antiserum also could neutralize the cytotoxic activities in Vero cell line by MTT and Neutral red assays. The results of the present study suggest that cocktail antiserum neutralizes the lethal activities in both in vitro and in vivo models using the antiserum against cobra venom and its individual venom proteins serum produced in rabbits.

Synthesis and Preliminary In-Vitro Cytotoxic Activity of Various Novel 4-(N-Substituted Benzothiazolyl) Amino-7-Methoxy-6-(3-Morpholinopropoxy) Quinazoline Derivatives.

A variety of 4-(N-substituted benzothiazolyl) amino-7-methoxy-6-(3-morpholinopropoxy) quinazoline were synthesized by using various N-substituted 2-amino benzothiazole, and substituted quinazoline. The structures of these compounds were confirmed by IR, Mass and 1HNMR spectral analysis. Newly synthesized compounds were tested for their In-Vitro Cytotoxic Activity using MTT assay. Results of the study indicated novel series of N-substituted (1, 3-benzothiazole)-7-methoxy-6-(3-morpholinopropoxy) quinazoline-4-yl-amine derivatives are potential class of anticancer agents.

Nonlipopolysaccharide component(s) of Lactobacillus acidophilus stimulate(s) the production of interleukin-1 alpha and tumor necrosis factor-alpha by murine macrophages.

Previous studies in our laboratory suggested that Lactobacillus acidophilus strain DDS-1 (LA1) has a suppressive effect on chemically induced tumors in experimental animals. In an effort to understand the possible mechanisms underlying this effect, we investigated the ability of LA1 to induce the production of interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor-alpha (TNF-alpha), which have potent cytocidal and cytostatic effects on tumor cells. The mouse macrophage cell line RAW264.7 was incubated with live or heat-killed cells of four strains of L. acidophilus or Bifidobacterium bifidum. Escherichia coli was used as a source of lipopolysaccharide that is known to induce the above cytokines. The amount of the cytokines present in the culture fluid was quantitated by an enzyme-linked immunosorbent assay. LA1 induced the production of higher levels of IL-1 alpha and TNF-alpha than other lactobacilli and bifidobacteria. Stimulation of the production of the cytokines was not due to the lipopolysaccharide (LPS) component, since LPS at concentrations equivalent to, or 100-fold greater than, that of LA1 induced only negligible amounts of IL-1 alpha and TNF-alpha. These results reveal that non-LPS component(s) of LA1 stimulate(s) the production of IL-1 alpha and TNF-alpha by macrophages, indicating that this organism stimulates the production of immunologic factors.

Cypermethrin-induced in vivo alterations in the carbohydrate metabolism of freshwater fish, Labeo rohita.

Aspects of carbohydrate metabolism under in vivo conditions were analyzed in functionally different tissues of the freshwater fish, Labeo rohita, exposed to a lethal (LC50/96 hr = 5.24 micrograms liter-1) and sublethal concentration (0.52 micrograms liter-1) of cypermethrin for 4 days. All exposed fish exhibited a hyperglycemic condition. An increase in tissue lactate with a decrease in pyruvate, total carbohydrates, and glycogen contents was noted. Activity of lactate dehydrogenase was elevated, indicating a shift toward anaerobiosis. TCA cycle enzymes, namely succinate dehydrogenase and malate dehydrogenase, were inhibited. In most cases changes were more pronounced during a lethal exposure compared to sublethal exposure period. The data indicate that the fish has adopted a compensatory mechanism to derive energy during pyrethroid toxicosis.