Negative impacts of perishable waste biochar to Escherichia coli and exploring potential damage factors.

Agricultural application of pyrolysis­carbonized perishable wastes can target reduction treatment and resource utilization of the wastes. However, potential undesirable impact has rarely been assessed. In this study, the adverse effect of perishable waste biochars (PWB) from different pyrolysis temperatures on Escherichia coli (E. coli) was explored and the potential risk factors were further analyzed. The results showed that PWBs pyrolyzed at 350, 500, and 650 °C inhibited the growth of E. coli, and PWB pyrolyzed at 500 °C showed the most inhibition. The exposure to PWB damaged the antioxidative system, as revealed by the concentration-dependent increasing of intracellular ROS. In addition, the toxicity at the gene level in terms of cell division and growth inhibition, the damage of cell membrane, antioxidant system disturbance, and DNA damage occurred, resulting in loss of the cell rules of morphology and eventual death. According to our results, the inhibitory effect on the growth of E. coli was mainly caused by PWB solids, accounting for >70 %. The membrane disruption and oxidative damage of E. coli by PWB were possibly induced by the direct physical interaction between cell and char particles. The growth of E. coli can be partly influenced by PWB extraction solutions that varied between PWB types, due to the differences in pH, released DOC and the production of extracellular ∙OH. The exploration of these potential hazards could provide new insights into the fate and toxicity of PWB in the environment and help guide the safe and sustainable applications for PWB.

Pesticides in the environment: Degradation routes, pesticide transformation products and ecotoxicological considerations.

Among rising environmental concerns, emerging contaminants constitute a variety of different chemicals and biological agents. The composition, residence time in environmental media, chemical interactions, and toxicity of emerging contaminants are not fully known, and hence, their regulation becomes problematic. Some of the important groups of emerging contaminants are pesticides and pesticide transformation products (PTPs), which present a considerable obstacle to maintaining and preserving ecosystem health. This review article aims to thoroughly comprehend the occurrence, fate, and ecotoxicological importance of pesticide transformation products (PTPs). The paper provides an overview of pesticides and PTPs as contaminants of emerging concern and discusses the modes of degradation of pesticides, their properties and associated risks. The degradation of pesticides, however, does not lead to complete destruction but can instead lead to the generation of PTPs. The review discusses the properties and toxicity of PTPs and presents the methods available for their detection. Moreover, the present study examines the existing regulatory framework and suggests the need for the development of new technologies for easy, routine detection of PTPs to regulate them effectively in the environment.

Endogenous ureides are employed as a carbon source in Arabidopsis plants exposed to carbon starvation conditions.

Ureides, the degraded products of purine catabolism in Arabidopsis, have been shown to act as antioxidant and nitrogen sources. Herein we elucidate purine degraded metabolites as a carbon source using the Arabidopsis Atxdh1, Ataln, and Ataah knockout (KO) mutants vis-à-vis wild-type (WT) plants. Plants were grown under short-day conditions on agar plates containing half-strength MS medium with or without 1% sucrose. Notably, the absence of sucrose led to diminished biomass accumulation in both shoot and root tissues of the Atxdh1, Ataln, and Ataah mutants, while no such effect was observed in WT plants. Moreover, the application of sucrose resulted in a reduction of purine degradation metabolite levels, specifically xanthine and allantoin, predominantly within the roots of WT plants. Remarkably, an increase in proteins associated with the purine degradation pathway was observed in WT plants in the presence of sucrose. Lower glyoxylate levels in the roots but not in the shoot of the Atxdh1 mutant in comparison to WT, were observed under sucrose limitation, and improved by sucrose application in root, indicating that purine degradation provided glyoxylate in the root. Furthermore, the deficit of purine-degraded metabolites in the roots of mutants subjected to carbon starvation was partially mitigated through allantoin application. Collectively, these findings signify that under conditions of sucrose limitation and short-day growth, purines are primarily remobilized within the root system to augment the availability of ureides, serving as an additional carbon (as well as nitrogen) source to support plant growth.

Chromiomics: Chromium detoxification and approaches for engineering tolerance in plants.

Chromium (Cr) is a heavy metal that poses a grave threat to the ecosystem including plants. Chromium is very harmful to plants due to its effects on many physiological and metabolic pathways culminating in a negative impact on plant's growth, development, and ability to take up nutrients. Plants have developed physiological, biochemical, and molecular ways of defense against Cr, such as by augmenting antioxidant potential to reduce reactive oxygen species (ROS). A number of genes have been discovered to play a significant role in the defense mechanisms of plants against Cr, for example, genes associated with the activation of phytochelatins, metallothioneins, and those of enzymes like glutathione-S-transferases. Along with this, a few miRNAs have been found to be associated in alleviating Cr stress and, to augment plant tolerance by controlling transcription factors, HSPs, and the expression of a few proteins and hormones. Defense pathway genes and miRNAs have been used for the generation of transgenic phytoremediator plants. Not only do the transgenic plants have a higher tolerance to Cr, but they also act as hyperaccumulators for Cr and have the potential to remediate other heavy metals. This article describes about environmental Cr contamination, Cr effects on plants, different genes and miRNAs involved in Cr stress mitigation and use of candidate genes, microRNAs for creating transgenic plant systems for phytoremediation, and the applications of CRISPR technology. It is expected that the integration of omics approach and advanced genomics will offer scope for more effective phytoremediation of Chromium in the coming years.

Overexpression of OsTIP1;2 confers arsenite tolerance in rice and reduces root-to-shoot translocation of arsenic.

Tonoplast Intrinsic Proteins (TIPs) are vital in transporting water and solutes across vacuolar membrane. The role of TIPs in the arsenic stress response is largely undefined. Rice shows sensitivity to the arsenite [As[III]] stress and its accumulation at high concentrations in grains poses severe health hazards. In this study, functional characterization of OsTIP1;2 from Oryza sativa indica cultivar Pusa Basmati-1 (PB-1) was done under the As[III] stress. Overexpression of OsTIP1;2 in PB-1 rice conferred tolerance to As[III] treatment measured in terms of enhanced shoot growth, biomass, and shoot/root ratio of overexpression (OE) lines compared to the wild-type (WT) plants. Moreover, seed priming with the IRW100 yeast cells (deficient in vacuolar membrane As[III] transporter YCF1) expressing OsTIP1;2 further increased As[III] stress tolerance of both WT and OE plants. The dithizone assay showed that WT plants accumulated high arsenic in shoots, while OE lines accumulated more arsenic in roots than shoots thereby limiting the translocation of arsenic to shoot. The activity of enzymatic and non-enzymatic antioxidants also increased in the OE lines on exposure to As[III]. The tissue-specific localization showed OsTIP1;2 promoter activity in root and root hairs, indicating its possible root-specific function. After As[III] treatment in hydroponic medium, the arsenic translocation factor (TF) for WT was around 0.8, while that of OE lines was around 0.2. Moreover, the arsenic content in the grains of OE lines reduced significantly compared to WT plants.

Vetiver grass cleans up arsenic contaminated field for subsequent safe cultivation of rice with low arsenic in grains: A two year field study.

The presence of high concentrations of arsenic (As) in agricultural soils and its subsequent accumulation in rice crop is a serious issue threatening sustainability of agriculture and human health. In the present work, remediation of As contaminated field in Nadia, West Bengal, India was done through the cultivation of Vetiver (Vetiveria zizanoides L. Nash) and the same field was subsequently used for rice (Oryza sativa L.) cultivation. The results showed that V. zizanoides could reduce As concentrations in the field to bring it lower than the maximum permissible limit (20 mg kg-1) in 11 months' time. The rice plants grown in remediated field showed improvement in growth and photosynthesis parameters as compared to that of contaminated field. Importantly, yield related parameters (filled seed, 1000 grain weight, number of panicles etc.) were also significantly higher in remediated field than that in contaminated field. Arsenic concentration in roots, shoot, husk and grains of rice was found to be significantly lower in remediated field than in contaminated field. Grain As decreased from 0.75 to 0.77 µg g-1 dw in contaminated field to 0.15-0.18 µg g-1 dw. In conclusion, replacing rice for single year with V. zizanoides crop can significantly remediate the field and can be a viable option.

Nanotechnology in sustainable agriculture: A double-edged sword.

Nanotechnology is a rapidly developing discipline that has the potential to transform the way we approach problems in a variety of fields, including agriculture. The use of nanotechnology in sustainable agriculture has gained popularity in recent years. It has various applications in agriculture, such as the development of nanoscale materials and devices to boost agricultural productivity, enhance food quality and safety, improve the efficiency of water and nutrient usage, and reduce environmental pollution. Nanotechnology has proven to be very beneficial in this field, particularly in the development of nanoscale delivery systems for agrochemicals such as pesticides, fertilizers, and growth regulators. These nanoscale delivery technologies offer various benefits over conventional delivery systems, including better penetration and distribution, enhanced efficacy, and lower environmental impact. Encapsulating agrochemicals in nanoscale particles enables direct delivery to the targeted site in the plant, thereby reducing waste and minimizing off-target effects. Plants are fundamental building blocks of all ecosystems and evaluating the interaction between nanoparticles (NPs) and plants is a crucial aspect of risk assessment. This critical review therefore aims to provide an overview of the latest advances regarding the positive and negative effects of nanotechnology in agriculture. It also explores potential future research directions focused on ensuring the safe utilization of NPs in this field, which could lead to sustainable development. © 2024 Society of Chemical Industry.

An evaluation of arsenic contamination status and its potential health risk assessment in villages of Nadia and North 24 Parganas, West Bengal, India.

The present study was conducted to evaluate the arsenic (As) contamination and possible associated health hazards to exposed population in four villages of two districts (Nadia and North 24 Parganas) of West Bengal, India. The study included two villages each from Nadia (Jaguli and Kugacchi) and North 24 Parganas (Chamta and Byaspur) districts. Groundwater, surface water, soil, rice grains and rice-based food samples were collected from these villages. The results revealed the presence of As in high concentrations in groundwater (35.00 to 186.00 µg L-1), surface water (30.00 to 61.00 µg L-1), soil (46.17 to 66.00 mg kg-1), rice grains (0.017 to 1.27 µg g-1) and rice-based food products (0.012 to 0.40 µg g-1). The maximum As levels were recorded in all types of samples collected from Kugacchi village. The rice grain samples included high-yielding and local varieties, and the level of As in high-yielding varieties was found to be higher (0.72 to 1.27 µg g-1) than in local varieties (0.25 to 1.06 µg g-1). The data of As concentrations was used for understanding the hazard quotient (HQ) and incremental lifetime cancer risk (ILCR) to the As-exposed population, and significant non-carcinogenic and carcinogenic risks were revealed considering consumption of rice grains at 400 g per day. The study demonstrates the severity of As contamination in the surveyed villages, which may pose a hindrance to attainment of sustainable development goals (SDGs) by 2030 and proposes the implementation of requisite safety measures.

Bacterial consortium (Priestia endophytica NDAS01F, Bacillus licheniformis NDSA24R, and Priestia flexa NDAS28R) and thiourea mediated amelioration of arsenic stress and growth improvement of Oryza sativa L.

The present study analyzed the effects of individual microbes and their consortium (Priestia endophytica NDAS01F, Bacillus licheniformis NDSA24R, and P. flexa NDAS28R) either alone or in interaction with thiourea (TU) on growth and responses of rice plants subjected to As stress (50 mg kg-1 in soil) in a pot experiment. The bacteria used in the experiment were isolated from As contaminated fields of Nadia, West Bengal and showed significant As removal potential in in vitro experiment. The results revealed significant growth improvement, biomass accumulation, and decline in malondialdehyde levels in rice plants in bacterial and TU treatments as compared to control As treatment. The best results were observed in a bacterial consortium (B1-2-3), which induced a profound increase of 65%, 43%, 127% and 83% in root length, shoot length, leaf width and fresh weight, respectively. Sulfur metabolism and cell wall synthesis were stimulated upon bacterial and TU amendment in plants. The maximum reduction in As concentration was observed in B2 in roots (-55%) and in B1-2-3 in shoot (-83%). The combined treatment of B1-2-3 + TU proved to be less effective as compared to that of B1-2-3 in terms of As reduction and growth improvement. Hence, the usage of bacterial consortium obtained in the present work is a sustainable approach, which might find relevance in field conditions to achieve As reduction in rice grains and to attain higher growth of plants without the need for additional TU supplementation.

Heavy metal (loid)s phytotoxicity in crops and its mitigation through seed priming technology.

Unexpected bioaccumulation and biomagnification of heavy metal(loid)s (HMs) in the environment have become a predicament for all living organisms, including plants. The presence of these HMs in the plant system raised the level of reactive oxygen species (ROS) and remodeled several vital cellular biomolecules. These lead to several morphological, physiological, metabolic, and molecular aberrations in plants ranging from chlorosis of leaves to the lipid peroxidation of membranes, and degradation of proteins and nucleic acid including the modulation of the enzymatic system, which ultimately affects the plant growth and productivity. Plants are equipped with several mechanisms to counteract the HMs toxicity. Among them, seed priming (SP) technology has been widely tested with the use of several inorganic chemicals, plant growth regulators (PGRs), gasotransmitters, nanoparticles, living organisms, and plant leaf extracts. The use of these compounds has the potential to alleviate the HMs toxicity through the strengthening of the antioxidant defense system, generation of low molecular weight metallothionein's (MTs), and phytochelatins (PCs), and improving seedling vigor during early growth stages. This review presents an account of the sources, uptake and transport, and phytotoxic effects of HMs with special attention to different mechanism/s, occurring to mitigate the HMs toxicity in plants employing SP technology.Novelty statement: To the best of our knowledge, this review has delineated the consequences of HMs on the crucial plant processes, which ultimately affect plant growth and development. This review also compiled the up to dated information on phytotoxicity of HMs through the use of SP technology, this review discussed how different types of SP approaches help in diminishing the concentration HMs in plant systems. Also, we depicted mechanisms, represent how HMs transport and their actions on cellular levels, and emphasized, how diverse SP technology effectiveness in the mitigation of plants' phytotoxicity in unique ways.

Recent advances in arsenic mitigation in rice through biotechnological approaches.

Arsenic (As) is a major threat to the environment and human health due to its toxicity and carcinogenicity. Occurrence of alarming concentrations of As in water and soil leads to its bioaccumulation in crops which is a major health concern globally. Rice (Oryza sativa) is a staple food for a large population staying in As contaminated areas so, it is of utmost importance to reduce As levels in rice, especially grains. Amongst several strategies in practice, biotechnology may provide an effective option to reduce As accumulation in rice grains. Genetic engineering can be a viable approach to exploit potential genes playing roles in As metabolism pathway in plants. Besides, developing low As accumulating rice varieties through breeding is also an important area. Identifying genotypic variation in rice is a crucial step toward the development of a safe rice cultivar for growing in As-affected areas. Significant genotypic variation has been found in rice varieties for As accumulation in grains and that is attributable to differential expression of transporters, radial oxygen loss, and other regulators of As stress. This review provides recent updates on the research advances leading to transgenic and breeding approaches adopted to reduce As levels in rice, especially grains.

Arsenic (As) contamination in water, soil, and crops is creating a difficult situation for the large population across the globe. Various efforts are being made to reduce As levels in rice as it is a staple crop. This review presents recent biotechnological advances toward the development of low As accumulating rice. The review shall be an important information resource for the readers on the topic.

Comparative evaluation of cadmium phytoremediation potential of five varieties of Helianthus annuus L.

Helianthus annuus is a potential metal accumulator plant, which can find application in cadmium (Cd) phytoremediation and provide economic gains in terms of oil yield. This study is focused on Cd accumulation analysis, physiological and biochemical responses of five varieties of H. annuus (DRSF-108, DRSF-113, LSFH-171, Phule Bhaskar and KBSH-44). Plantlets of all varieties were treated with various Cd concentrations (10, 50, 100, 300 and 500 mg kg-1) for 20, 40 and 60 days. DRSF-108 showed the maximum total Cd accumulation (430.52 mg kg-1) in whole plant while minimum accumulation was observed in KBSH-44 (150.66 mg kg-1) at 500 mg kg-1 Cd after 60 days. The highest level of proline and polyphenol in DRSF-108 were 27.206 µmol g-1 fw and 6.86 mg g-1 fw, respectively. Antioxidant enzymes (catalase, ascorbate peroxidase and glutathione reductase) also showed increased activity in response to Cd treatment. SEM-EDX analysis of potential accumulator genotype, DRSF-108, showed the distribution of intracellular Cd into plant tissues. Therefore, it is concluded that among five varieties, DRSF-108 was the most potential Cd accumulator and had a higher capacity for Cd tolerance compared to other varieties. Our findings may allow us to extend variety DRSF-108 for sustainable farming and Cd remediation.

Phytoremediation of environmental contaminants is a promising strategy, however, the success depends on the use of suitable plants. Helianthus annuus is a good phytoremediator plant and the use of most promising genotype is desirable. This work evaluated five genotypes of H. annuus for cadmium accumulation and identified the best variety, DRSF-108 that can be used for cadmium remediation. The work shall pave way for field application of sunflower plants in cadmium remediation.

Receptor-like Kinases (LRR-RLKs) in Response of Plants to Biotic and Abiotic Stresses.

Plants live under different biotic and abiotic stress conditions, and, to cope with the adversity and severity, plants have well-developed resistance mechanisms. The mechanism starts with perception of the stimuli followed by molecular, biochemical, and physiological adaptive measures. The family of LRR-RLKs (leucine-rich repeat receptor-like kinases) is one such group that perceives biotic and abiotic stimuli and also plays important roles in different biological processes of development. This has been mostly studied in the model plant, Arabidopsis thaliana, and to some extent in other plants, such as Solanum lycopersicum, Nicotiana benthamiana, Brassica napus, Oryza sativa, Triticum aestivum, Hordeum vulgare, Brachypodium distachyon, Medicago truncatula, Gossypium barbadense, Phaseolus vulgaris, Solanum tuberosum, and Malus robusta. Most LRR-RLKs tend to form different combinations of LRR-RLKs-complexes (dimer, trimer, and tetramers), and some of them were observed as important receptors in immune responses, cell death, and plant development processes. However, less is known about the function(s) of LRR-RLKs in response to abiotic and biotic stresses. Here, we give recent updates about LRR-RLK receptors, specifically focusing on their involvement in biotic and abiotic stresses in the model plant, A. thaliana. Furthermore, the recent studies on LRR-RLKs that are homologous in other plants is also reviewed in relation to their role in triggering stress response processes against biotic and abiotic stimuli and/or in exploring their additional function(s). Furthermore, we present the interactions and combinations among LRR-RLK receptors that have been confirmed through experiments. Moreover, based on GENEINVESTIGATOR microarray database analysis, we predict some potential LRR-RLK genes involved in certain biotic and abiotic stresses whose function and mechanism may be explored.

Pyrolysis temperature affects the inhibitory mechanism of biochars on the mobility of extracellular antibiotic resistance genes in saturated porous media.

The migration of extracellular antibiotic resistance genes (eARGs) in porous media is an important pathway for ARGs to spread to the subsoil and aquifer. Biochar (BC) has been widely used to reduce the mobility of soil contaminants, however, its effect on the mobility of eARGs in porous media and the mechanisms are largely unknown. Herein, the effects of BCs synthesized from wheat straw and corn straw at two pyrolysis temperatures (300 °C and 700 °C) on the transport of plasmids-carried eARGs in sand column were investigated. The BC amendments all significantly decreased the mobility of eARGs in the porous medium, but the mechanism varied with pyrolysis temperature. The higher temperature BCs had a stronger irreversible adsorption of plasmids and greatly enhanced the attachment and straining effects on plasmids during transport, thus more effectively inhibited the mobility of eARGs. The lower temperature BCs had weaker adsorption, attachment, and straining effects on plasmids, but induced generation of hydroxyl radicals in the porous medium and thereby fragmented the plasmids and hindered the amplification of eARGs. These findings are of fundamental significance for the potential application of BC in controlling the vertical spread of eARGs in soil and vadose zones.

Comprehensive illustration of transcriptomic and proteomic dataset for mitigation of arsenic toxicity in rice (Oryza sativa L.) by microbial consortium.

The present article represents the data for analysis of microbial consortium (P.putida+C.vulgaris) mediated amelioration of arsenic toxicity in rice plant. In the current study the transcriptome profiling of treated rice root and shoot was performed by illumina sequencing (Platform 2000). To process the reads and to analyse differential gene expression, Fastxtoolkit, NGSQCtoolkit, Bowtie 2 (version 2.1.0), Tophat program (version 2.0.8), Cufflinks and Cuffdiff programs were used. For Proteome profiling, total soluble proteins in shoot of rice plant among different treatments were extracted and separated by 2D poly acrylamide gel electrophoresis (PAGE) and then proteins were identified with the help of MALDI-TOF/TOF. In gel based method of protein identification, the isoelectric focusing machine (IPGphor system,Bio-Rad USA), gel unit (SDS-PAGE) and MALDI-TOF/TOF (4800 proteomic analyzer Applied Biosystem, USA) were used for successful separation and positive identification of proteins. To check the differential abundance of proteins among different treatments, PDQuest software was used for data analysis. For protein identification, Mascot search engine (http://www.matrixscience.com) using NCBIprot/SwissProt databases of rice was used. The analyzed data inferred comprehensive picture of key genes and their respective proteins involved in microbial consortium mediated improved plant growth and amelioration of As induced phyto-toxicity in rice. For the more comprehensive information of data, the related full-length article entitled "Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling" may be accessed.

An assessment of various potentially toxic elements and associated health risks in agricultural soil along the middle Gangetic basin, India.

The present study analysed the levels of potentially toxic elements along with physico-chemical properties of agricultural soil samples (n = 59) collected from fields situated along the path of river Ganga in the middle Gangetic floodplain in two districts, Ballia and Ghazipur. Arsenic (As), chromium (Cr), copper (Cu), nickel (Ni), zinc (Zn), lead (Pb), iron (Fe) and manganese (Mn) levels were analysed by Wavelength Dispersive-X-Ray Fluorescence Spectroscopy (WD-XRF) and the associated health risks along with diverse indices were calculated. The mean concentrations of As, Cu, Cr, Pb, Zn and Ni were found to be 15, 42, 85, 18, 87 and 47 mg kg-1, respectively in Ballia and 13, 31, 73, 22, 77 and 34 mg kg-1, respectively in Ghazipur. Physico-chemical properties like pH, ORP and organic matter were found to be 7.91, 209 and 1.20, respectively in Ballia and 8.51, 155 and 1.25, respectively in Ghazipur. The calculated health quotient (HQ) for all the elements was observed to be within the threshold value of one, however with few exemptions. Therefore, the present study showcases the contamination of potentially toxic elements in agricultural fields and possible health hazards for people.

Application of Pteris vittata L. for phytoremediation of arsenic and biomonitoring of the process through cyto-genetic biomarkers of Trigonella foenum-graecum L.

The arsenic (As) contamination demands its remediation from the environment which is naturally possible by the application of Pteris vittata L. However, biomonitoring of phytoremediation potential of P. vittata at chromosomal and DNA level is still meager. The present study was designed to biomonitor the phytoremediation efficiency of P. vittata through phytotoxic and cyto-genotoxic biomarkers assessment using Trigonella foenum-graecum L. (Fenugreek; Methi) as test system. Study revealed hyperaccumulation potential of P. vittata which extracted arsenic in its tissues. Biomonitoring evaluation depicted that phytotoxic damage was reduced in Trigonella exposed to remediated soil, which was revealed through reduced electrolyte leakage, hydrogen peroxide and MDA content. Moreover, cyto-genetic endpoints like mitotic depression (44.03%), relative abnormality rate (16.6%) and chromosomal abnormality frequency (1.06%) were also lesser in test plants grown in remediated soil compared to those grown in non-remediated soil. Along with this various chromosomal aberrations like stickiness, breaks, laggards, bridges, fragmentations and micronuclei were also augmented in test plants exposed to non-remediated arsenic enriched soil. It was evident that arsenic enriched soil caused toxicity to plants in dose-dependent manner that was assessable through the analysis of biochemical parameters and cyto-genetic biomarkers. The cyto-genetic biomarkers are very efficient, simple and non-expensive tools to biomonitor arsenic toxicity at chromosomal as well as DNA level to assess the remediation potential of P. vittata in field conditions.

Genome-wide profiling of drought-tolerant Arabidopsis plants over-expressing chickpea MT1 gene reveals transcription factors implicated in stress modulation.

Drought, a major abiotic limiting factor, could be modulated with in-built reprogramming of plants at molecular level by regulating the activity of plant developmental processes, stress endurance and adaptation. The transgenic Arabidopsis thaliana over-expressing metallothionein 1 (MT1) gene of desi chickpea (Cicer arietinum L.) was subjected to transcriptome analysis. We evaluated drought tolerance of 7 days old plants of Arabidopsis thaliana in both wild-type (WT) as well as transgenic plants and performed transcriptome analysis. Our analysis revealed 24,737 transcripts representing 24,594 genes out of which 5,816 were differentially expressed genes (DEGs) under drought conditions and 841 genes were common in both genotypes. A total of 1251 DEGs in WT and 2099 in MT1 were identified in comparison with control. Out of the significant DEGs, 432 and 944 were upregulated, whereas 819 and 1155 were downregulated in WT and MT1 plants, respectively. The physiological and molecular parameters involving germination assay, root length measurements under different stress treatments and quantitative expression analysis of transgenic plants in comparison to wild-type were found to be enhanced. CarMT1 plants also demonstrated modulation of various other stress-responsive genes that reprogrammed themselves for stress adaptation. Amongst various drought-responsive genes, 24 DEGs showed similar quantitative expression as obtained through RNA sequencing data. Hence, these modulatory genes could be used as a genetic tool for understanding and delineating the mechanisms for fine-tuning of stress responses in crop plants.

Antioxidant enzymes and transporter genes mediate arsenic stress reduction in rice (Oryza sativa L.) upon thiourea supplementation.

Thiourea (TU) is a chemo-priming agent and non-physiological reactive oxygen species (ROS) scavenger whose application has been found to reduce As accumulation in rice grains along with improved growth and yield. The present field study explored TU-mediated mechanistic changes in silicon (Si) assimilation in root/shoot, biochemical and molecular mechanisms of arsenic (As) stress amelioration in rice cultivars. Gosai and Satabdi (IET-4786) rice cultivars were selected for field experiment at three different places; control field and two other As contaminated experimental fields (EF1 and EF2) in West Bengal, India. The average As reduction was observed to be 9.5% and 19.8% whereas the yield increment was 8.8% and 17.7% for gosai and satabdi, respectively among all the three experimental fields. The positive interrelation was also observed between improved internal ultrastructure anatomy and enhanced Si assimilation (36%-423%) upon TU application. The level of photosynthetic pigments was increased by 29.8%-99.2%. Further, activities of antioxidant enzymes were harmonically altered in TU supplemented plants. The expression of various As related transporter genes in flag leaf and developing grains (inflorescence) was changed in both the rice cultivars (gosai and satabdi). It was also presumably responsible for observed As reduction in grains. Thus, TU application was found to be an efficient and sustainable agronomic practice for amelioration of As toxicity in rice plants in As contaminated field conditions.