The 2022 West Nile Virus Season in Greece; A Quite Intense Season.

Since 2010, the West Nile virus (WNV) has been established in Greece. We describe the epidemiology of diagnosed human WNV infections in Greece with a focus on the 2022 season. During the transmission period, clinicians were sending samples from suspected cases for testing. Active laboratory-based surveillance was performed with immediate notification of diagnosed cases. We collected clinical information and interviewed patients on a timely basis to identify their place of exposure. Besides serological and molecular diagnostic methods, next-generation sequencing was also performed. In 2022, 286 cases of WNV infection were diagnosed, including 278 symptomatic cases and 184 (64%) cases with neuroinvasive disease (WNND); 33 patients died. This was the third most intense season concerning the number of WNND cases, following 2018 and 2010. Most (96%) cases were recorded in two regions, in northern and central Greece. The virus strain was a variant of previous years, clustering into the Central European subclade of WNV lineage 2. The 2022 WNV season was quite intense in Greece. The prompt diagnosis and investigation of cases are considered pivotal for the timely response, while the availability of whole genome sequences enables studies on the molecular epidemiology of the disease.

West Nile virus in humans, Greece, 2018: the largest seasonal number of cases, 9 years after its emergence in the country.

BackgroundHuman cases of West Nile virus (WNV) infection are recorded since 2010 in Greece, with seasonal outbreaks occurring almost annually. Enhanced surveillance has been implemented since 2010, to promptly characterise cases' temporal and geographical distribution and inform authorities for implementation of appropriate measures (mosquito control, health education, blood safety).AimWe describe the epidemiology of WNV human infections in Greece focusing on the 2018 season.MethodsThe National Public Health Organization advised physicians to test all suspect WNV infection cases and refer samples to reference laboratories. Laboratories notified diagnosed cases on a daily basis. Treating physicians, patients, and infected blood donors were interviewed within 48 hours after diagnosis and the probable infection location was identified. Hospitalised cases were followed up until discharge.ResultsA total of 317 autochthonous WNV infection cases were diagnosed in 2018. Among them, 243 cases had neuroinvasive disease (WNND), representing a 23% increase of WNND cases compared with 2010, the previous most intense season. There were 51 deaths. Cases started occurring from week 22, earlier than usual. Both rural and urban areas were affected, with 86 (26% of the total) municipalities belonging to seven (54% of the total) regions recording cases. Two major epicentres were identified in Attica and Central Macedonia regions.ConclusionsThe largest number of human cases of WNV infection ever recorded in Greece occurred in 2018, with a wide geographical distribution, suggesting intense virus circulation. Enhanced surveillance is vital for the early detection of human cases and the prompt implementation of response measures.

The first Programmefood and nutrition security, impact, resilience, sustainability and transformation: Review and future directions.

The FIRST Program - Food and Nutrition Security Impact, Resilience, Sustainability and Transformation, is a partnership between the European Union and FAO. The EU and FAO launched a stocktaking exercise to assess progress in improving food security and nutrition in 24 FIRST priority countries. This paper presents the results of a quantitative analyses, literature review, country reports and a May 2019 consensus workshop that were the basis of identifying issues that must be addressed for FIRST countries going forward. Seven thematic areas were emphasized as essential for meeting the targets in SDG2 - zero hunger. These factors include reinventing agriculture; unleashing the private sector; gender equity; decentralization of programs; multi sector concepts within a sector approach; prioritization; data, and political process and governance.

The cost of blood collection in Greece: an economic analysis.

OBJECTIVE: The goal of this study was to estimate the cost of production of 1 unit of blood from a National Health Service perspective in Greece. METHODS: In agreement with guidelines, the cost of blood production in this study accounted only for the resources expended for collection, processing, laboratory testing, and storage. Hence, the costs associated with donor recruitment, pretransfusion preparation, transfusion administration, follow-up management of adverse events, and other long-term relevant costs were not taken into consideration. The indirect cost of blood donations for donors (productivity loss) was also considered. A questionnaire was used to collect data regarding personnel time, annual blood quantities collected, percentage of wastage, utilization of consumables, institutional overhead, information technology expenditure, medical equipment utilized, nuclear acid tests, and other factors. Data gathered by 53 hospitals across the country were assessed. A model was constructed with economic data collected by the National School of Public Health and the Ministry of Health. All data refer to the year 2013. RESULTS: The weighted mean direct cost of producing 1 unit of blood was estimated at €131.49 (SD, €22.12; minimum/maximum, €94.96-€239.20). The mean total indirect cost was estimated at €34 per unit of blood. The cost distribution was positively skewed (skewness, 1.642 [0.327]). The major cost component was the cost of personnel, accounting for 32.5% of total costs, and the average of blood unit wastage was estimated at 4.90%. There were no differences between the cost of producing 1 unit of blood in Athens compared with the rest of the country (Mann-Whitney test, P = 0.341). CONCLUSIONS: This study suggests that the cost of producing 1 unit of blood is not insignificant. These figures need to be complemented with those concerning the cost of transfusion to have a complete picture of producing and using 1 unit of blood locally.

Intraoral registration coping formation using an interim restoration as a matrix.

An intraoral procedure for registration coping fabrication is described. The indirectly constructed shell of the interim fixed partial denture is used as a matrix, and a light-cured resin is added directly to form the copings. The proposed technique reduces the total number of clinical sessions and can be useful in cases when tooth preparations and final impressions can be completed at the same appointment.

Intraoral acrylic resin coping fabrication for making interocclusal records.

A safe and accurate method for the fabrication of acrylic resin copings to be used for interocclusal registration is described. Copings are fabricated on vital prepared teeth using visible light-cured acrylic resin, and the records are then obtained with autopolymerizing acrylic resin. This allows for an interocclusal recording to be obtained at the final impression appointment.

A technique to obtain stable centric occlusion records using impression plaster.

Recording the maxillomandibular relationship is often a challenge. In situations where there is vertical support but no horizontal stability, and the space between posterior teeth of opposing jaws is not adequate for an interocclusal record, impression plaster can be used. Despite limited clinical use, impression plaster is known for its stability. With this technique, the interocclusal record is obtained by applying the material to the buccal surfaces of the posterior teeth, resulting in a precise and stable cast relationship.