Ultrasound-assisted extraction and concentration of phenolic compounds from jabuticaba sabará (Plinia peruviana (Poir.) Govaerts) peel by nanofiltration membrane.

Jabuticaba peel, rich in antioxidants, offering health benefits. In this study, the extraction of phenolic compounds from jabuticaba peel using ultrasound-assisted (UA) and their subsequent concentration by nanofiltration (NF) employing a polyamide 200 Da membrane was evaluated. The UA extractions were conducted using the Central Composite Rotatable Design (CCRD) 22 methodology, with independent variables extraction time (11.55 to 138 min) and temperature (16.87 to 53.3 °C), and fixed variables mass to ethanol solution concentration at pH 1.0 (1:25 g/mL), granulometry (1 mm), and ultrasonic power (52.8 W). The maximum concentrations obtained were 700.94 mg CE/100 g for anthocyanins, 945.21 mg QE/100 g for flavonoids, 133.19 mg GAE/g for phenols, and an antioxidant activity IC50 of 24.36 µg/mL. Key phenolic compounds identified included cyanidin-3-glucoside, delphinidin-3-glucoside, and various acids like syringic and gallic. NF successfully concentrated these compounds, enhancing their yield by up to 45%. UA and NF integrate for sustainable extraction.

Tyrosinase-based nanobiosensor for environmental monitoring of hormones in river water.

This study explores the application of a tyrosinase cantilever nanobiosensor for detecting 17ß-estradiol and estrone in typical water systems. The physical-chemical parameters of water were evaluated within the Tigre River micro-basin in Erechim, RS, to determine water potability for urban populations. Water clarity, conductivity, and pH levels were essential markers, adhering to recognized standards for water quality and human consumption. The cantilever nanobiosensor demonstrated strong sensitivity and a broad linear range, with a limit of detection (<0.00051 ppb) surpassing other enzymatic biosensors and covering a range of 0.0001-100 ppb. The real water sample quality investigated in relation to contamination with 17ß-estradiol and estrone by nanobiosensor showed values below the LOD for both compounds. Recovery studies demonstrated the reliability of the nanobiosensor. Selectivity tests indicated minimal interference from structurally similar substances. This study validates the nanobiosensor's potential for environmental monitoring and hormone detection, aligning with standard practices.

Bioactive Compounds from Artichoke and Application Potential.

Cynara cardunculus L. var. scolymus, known as the artichoke, originated in the Mediterranean region and is now cultivated in several countries. The artichoke has leaves, a stem, and a head, also called a floral capitulum, covered with green and pointed bracts. It is rich in polyphenols, flavonoids, anthocyanins, phenolic compounds, inulin, coumarins, terpenes, dietary fibre, enzymes, polysaccharides, minerals and vitamins, and therefore has a wide range of uses, including in the food industry, medicine and biofuels. Several studies have shown that artichokes have properties such as antioxidant, anti-inflammatory, antimicrobial, anticancer, hypocholesterolaemic, anti-HIV, cardioprotective, hepatoprotective and lipid-lowering effects. The aim of this study is to provide a literature review on the phytochemical composition, bioactivity and applications, focusing on the methods of extraction, purification and concentration of enzymes present in artichoke.

Differentiating True and False Cinnamon: Exploring Multiple Approaches for Discrimination.

This study presents a comprehensive literature review that investigates the distinctions between true and false cinnamon. Given the intricate compositions of essential oils (EOs), various discrimination approaches were explored to ensure quality, safety, and authenticity, thereby establishing consumer confidence. Through the utilization of physical-chemical and instrumental analyses, the purity of EOs was evaluated via qualitative and quantitative assessments, enabling the identification of constituents or compounds within the oils. Consequently, a diverse array of techniques has been documented, encompassing organoleptic, physical, chemical, and instrumental methodologies, such as spectroscopic and chromatographic methods. Electronic noses (e-noses) exhibit significant potential for identifying cinnamon adulteration, presenting a rapid, non-destructive, and cost-effective approach. Leveraging their capability to detect and analyze volatile organic compound (VOC) profiles, e-noses can contribute to ensuring authenticity and quality in the food and fragrance industries. Continued research and development efforts in this domain will assuredly augment the capacities of this promising avenue, which is the utilization of Artificial Intelligence (AI) and Machine Learning (ML) algorithms in conjunction with spectroscopic data to combat cinnamon adulteration.

The moderating role of macronutrient intake in relation to body composition and genotoxicity: A study with gym users.

In a cross-sectional study of gymnasium users (both sexes, ages = 41.9 ± 14.8 years), we examined the moderating role of macronutrient intake in relation to body composition and genotoxicity. A questionnaire was administered to evaluate characteristics of the participants. To assess macronutrient consumption, we used 24-h food recalls on three non-consecutive days. Body composition (body fat percentage and muscle mass) was evaluated with a bioimpedance scale. Genotoxicity was assessed with the buccal micronucleus cytome assay. Multiple linear regression models were applied, adjusting for age; sex; tobacco and alcohol consumption; and (with regard to exercise habits) frequency, training time, intensity, and types. Micronucleus frequency was directly associated with body fat and inversely associated with muscle mass. Our study shows that carbohydrate and fat intakes affect body fat percentage and micronucleus frequency in gymnasium users.

Association between the use of muscle-building supplements and DNA damage in resistance training practitioners.

OBJECTIVES: Little is known about the relationship between the supplements used for sport and safety, especially regarding the induction of genotoxicity. Therefore, more knowledge about a DNA damage possibly caused using sport supplements is necessary. The aim of this study was to investigate the potential association between the use of muscle-building supplements and DNA damage in resistance training practitioners. METHODS: Muscle-building supplements were classified into three categories based on evidence of efficacy and safety: Strong Evidence to Support Efficacy and Apparently Safe (SESEAS); Limited or Mixed Evidence to Support Efficacy (LMESE), and Little to No Evidence to Support Efficacy and/or Safety (LNESES). DNA damage was evaluated by the comet assay (DNA damage index and frequency) and buccal micronucleus by the cytome assay (micronuclei and nuclear buds). In the sequence, the adjusted analysis of covariance was performed. This study included 307 individuals ages 37.99 ± 13.95 y (52.1% men), of which 157 consumed supplements. RESULTS: The results of the comet assay revealed that participants who used supplements had higher DNA damage indexes (P = 0.018) and damage frequency (P = 0.045) than those who reported using no supplements. Moreover, the comet assay also indicated that the participants who used supplements classified into the SESEAS category presented the highest DNA damage index (P = 0.025) and frequency (P = 0.044) compared with those who used no supplements. However, we found no significant difference in the micronuclei and nuclear buds in the evaluated groups (P > 0.05). CONCLUSION: Supplement use is not associated with permanent damage, suggesting that SESEAS supplements are safe for consumption.

Malolactic fermentation of lactic acid bacteria isolated from southern Brazilian red wine.

The objective was to isolate lactic acid bacteria (LAB) from southern Brazil's wines and investigate their potential as starter cultures for malolactic fermentation (MLF) in Merlot (ME) and Cabernet Sauvignon (CS) wines through the fermentative capacity. The LAB were isolated from CS, ME, and Pinot Noir (PN) wines in the 2016 and 2017 harvests and evaluated for morphological (color and shape of the colonies), genetic, fermentative (increase in pH, acidity reduction, preservation of anthocyanins, decarboxylation of L-malic acid, yield of L-lactic acid, and content of reduced sugars), and sensory characteristics. Four strains were identified as Oenococcus oeni [CS(16)3B1, ME(16)1A1, ME(17)26, and PN(17)65], one as Lactiplantibacillus plantarum [PN(17)75], and one as Paucilactobacillus suebicus [CS(17)5]. Isolates were evaluated in the MLF and compared to a commercial strain (O. oeni), as well as a control (without inoculation and spontaneous MLF), and standard (without MLF). CS(16)3B1 and ME(17)26 isolates finished the MLF for CS and ME wines, respectively, after 35 days, similar to the commercial strain, and CS(17)5 and ME(16)1A1 isolates ended the MLF in 45 days. In the sensory analysis, ME wines with isolated strains received better scores for flavor and overall quality than the control. Compared to the commercial strain, CS(16)3B1 isolate obtained the highest scores for buttery flavor and taste persistence. CS(17)5 isolate received the higher scores for a fruity flavor and overall quality and the lowest for a buttery flavor. The native LAB displayed MLF potential, regardless of the year and grape species from which they were isolated.

Recent updates to microbial production and recovery of polyhydroxyalkanoates.

The increasing use of synthetic polymers and their disposal has raised concern due to their adverse effects on the environment. Thus, other sustainable alternatives to synthetic plastics have been sought, such as polyhydroxyalkanoates (PHAs), which are promising microbial polyesters, mainly due to their compostable nature, biocompatibility, thermostability, and resilience, making this biopolymer acceptable in several applications in the global market. The large-scale production of PHAs by microorganisms is still limited by the high cost of production compared to conventional plastics. This review reports some strategies mentioned in the literature aimed at production and recovery, paving the way for the bio-based economy. For this, some aspects of PHAs are addressed, such as synthesis, production systems, process control using by-products from industries, and advances and challenges in the downstream. The bioplastics properties made them a prime candidate for food, pharmaceutical, and chemical industrial applications. With this paper, it is possible to see that biodegradable polymers are promising materials, mainly for reducing the pollution produced by polymers derived from petroleum.

Technological characteristics of tofu from soybean coagulated with cardoon flower extract.

The objective of the present study was to develop tofu with soybean-water soluble extract coagulated with cardoon flower (F1) and magnesium chloride (MgCl2, F2). The produced tofu was characterized in terms of physical, chemical, microbiological, and sensory properties during 14 days of storage. The yield of F1 was higher (p < 0.05) (195 g/100 soybean seeds) than F2 (162 g/100 soybean seeds). F1 presented higher moisture, protein, acidity, syneresis, and lipids when compared with F2, and a reduction of these contents during the storage. F1 presented lower hardness, stickiness, springiness, and cohesiveness compared with F2. The acceptability of F1 showed a score of 6.00 and F2 of 4.68, and the purchase intention was 3.22 for F1 and 2.23 for F2. This study recommended the use of cardoon flower at 35% level as it has great potential as a coagulant for the elaboration of tofu with higher yield, and acceptability and reasonable purchasing intention.

Combination of chemical coagulation and membrane-based separation for dairy wastewater treatment.

An important factor resulted from the ascension of the milk and milk-based by-products production is many effluents directly released into the environment. The main objective of this study was to evaluate the efficiency of the combination of the chemical coagulation, with ferric chloride as a coagulant, and the membrane separation processes (MSP) and reverse osmosis (RO) processes in the treatment of effluents from a powdered milk dairy industry. To evaluate the effectiveness of the integration of these mechanisms, the characterization of the effluents was carried out through Total Nitrogen (Ntotal), Total Organic Carbon (TOC), Chemical Oxygen Demand (COD), color, pH, and turbidity analysis. Regarding the treatments with ferric chloride, the Ntotal removal was up to 55.7% (concentration of 1.2 g L- 1) and the color up to 50% (0.7 g L- 1). For the MSP and RO treatments, the color removal was up to 100% (1st RO), turbidity up to 100% (1st RO), COD up to 98.7% (3rd RO), and TOC up to 96.7% (3rd RO). Finally, the integration of the chemical coagulation and MSP processes was efficient for the treatment of dairy industry wastewater and provides the return of water in appropriate characteristics according to legislation.

Evaluation of packaging systems with O2-absorbers on quality of minimally processed soybean sprouts.

This study evaluated the influence of O2-absorbing sachets into different packages polyethylene terephthalate (PET - E1), low density polyethylene (LDPE - E2), and PET/LDPE (E3) on the quality of minimally processed soybean sprouts (MP-sprouts). The MP-sprouts were stored up to 12 days and characterized for physicochemical, microbiological and sensory attributes. The O2-absorbing sachet showed changes during exposure to the environment by the formation of amorphous iron hydroxide, which was observed by the analysis of functional groups and XRD. The packaging used in the study showed high transmittance and clarity, and low Haze, being a good feature for storing the MP product. All packaging systems showed an increase in the volume of absorbed oxygen (cm3) up to 9 days of storage. The physic-chemical characteristics of the MP-sprouts stored for 12 days were preserved in the different packages, showing no difference regarding the use of the O2-absorbing sachet. The O2-absorbing sachet not influenced the quality of MP-sprouts during the stored (12 days). The best visual aspect was observed in PET package (with and without O2-absorbing sachet) being classified as excellent, without color change. The MP-sprouts also fulfilled the microbiological quality standards and presented 75.11% acceptability and 72.40% purchase intention.

Influence of different categories of supplements on the body composition of resistance-training practitioners.

OBJECTIVE: The aim of this study was to investigate the influence of different categories of dietary supplements on the body composition of resistance-training practitioners. METHODS: Participants of both sexes and ages 20 to 59 y participated in this cross-sectional study. The use of dietary supplements was investigated and classified into three categories: sports foods, medical supplements, and ergogenic supplements. One-way analysis of covariance adjusted for age, type of exercise, frequency of exercise, total amount of daily exercise, and exercise intensity was used in the analysis. RESULTS: Of 427 participants, 278 (65%), were supplement consumers. Women who consumed sports foods had higher percentages of skeletal muscle (P = 0.014) and lower percentages of body fat than women who did not take supplements (P = 0.031). Furthermore, women who used medicinal supplements had greater visceral fat levels than women who did not (P = 0.019). No significant differences were found between type of supplement used and body composition among men (P > 0.05). CONCLUSIONS: Consuming sports foods was associated with higher percentages of skeletal muscle and lower percentages of body fat; women who used medical supplements had higher visceral fat levels.

Iron supplementation does not aggravate impaired glucose tolerance and sugar overload-induced genotoxicity in rats.

High sugar intake is a major risk factor for metabolic disorders. Genotoxicity is an important factor in diabetes onset, and iron (Fe) may be an aggravating element. However, this relationship is still poorly established. Thus, this study evaluated whether Fe supplementation could aggravate obesity, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats. A total of 24 rats were treated with different diets: standard diet (SD, n = 8), invert sugar overload (320 g/L, HSD, n = 8), or Fe plus invert sugar overload (2.56 mg/L of Fe2+, Fe-HSD, n = 8) for four months. After treatment, the Fe-HSD group showed no excessive weight gain or impaired glucose tolerance. DNA damage in blood, as assessed by comet assay, gradually increased in HSD during treatment (p < 0.001), whereas Fe-HSD showed a nonlinear increase in DNA damage. Moreover, Fe-HSD presented 0.6-fold more DNA damage compared with SD (p = 0.0055) in the 1st month of treatment. At months 2 and 3, results show a ≥ 1.4-fold increase in HSD and Fe-HSD DNA damage, respectively, compared with SD (p < 0.01). At the end of the experiment, only HSD DNA damage differed from SD (1.5-fold more, p = 0.0196). Fe supplementation did not aggravate the invert sugar-induced DNA damage (p > 0.05). In the pancreas, results showed no differences in DNA damage. Mutagenicity, evaluated by micronucleus testing, was not observed regardless of treatment (p = 0.428). Fe supplementation, in the evaluated concentration, did not aggravate weight gain, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats.

Micronuclei frequency and renal function markers in gym members: The moderating role of supplement intake.

This research investigates the moderating role of dietary supplement intake in the relationship between MNi frequency and renal markers in gym members. A cross-sectional study was carried out with gym members of all sexes, between 20 and 59 years of age, with data on supplement use obtained via questionnaire. Renal markers (urea and creatinine) were assessed by blood collection. Buccal mucosa cells were collected to assess MNi frequency by buccal micronucleus cytome assay. Moderation was tested using multiple linear regression models by PROCESS macro for SPSS. Results showed significant interactions for supplement use (p = 0.001) and supplement type, ergogenic (p = 0.003) and sports food (p = 0.003), with MNi for urea. For creatinine, only supplement use showed interaction with MNi frequency (p = 0.048). In conclusion, supplement intake is a moderator in the relationship between MNi frequency and renal function markers in gym members.

Active edible films based on green tea extract and gelatin for coating of fresh sausage.

The objective of this work was to develop, characterize and evaluate the application of active edible films based on gelatin and green tea extract in coating of fresh sausages. The green tea extract showed IC50 of 0.088 mg/mL and minimum inhibitory concentrations of 0.05 mg/mL for Listeria monocytogenes, 0.025 mg/mL for Staphylococcus aureus, 0.04 mg/mL for Escherichia coli, and >1.0 mg/mL for Salmonella enterica serovar Choleraesuis. The formulation with 15% (w/v) of gelatin and 30% (w/w) of glycerol showed better adhesion and appearance in the coating of the product. When using 1.0% of green tea extract, the lowest IC50, was obtained and the antioxidant activity was maintained for 35 days. There was a more accentuated decrease in pH and an increase in acidity and peroxide index in fresh sausages without film compared to those coated with the active film (1.0% of green tea extract) during storage. In addition, it was found that the use of active gelatin film (1.0% of green tea extract) kept the TBARS indexes of fresh sausage samples lower than the standard (without coating) and of films containing only gelatin, after 48 days of storage.

Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors.

In this study, polyaniline/poly(styrene sulfonate) (PANI/PSS) nanocomposite and polyaniline·silver (PANI·Ag) nanohybrid thin films were obtained in cantilever nanosensors surface. The developed films were characterized in relation to topography, roughness, thickness, height, and structural properties. The topography study revealed that both films have a globular morphology, thickness and height in nanoscale. The gas sensing performance was investigated for sexual pheromone from the neotropical brown stink bug, Euschistus heros (F.). The sensitivities of both nanosensors based on PANI/PSS nanocomposite and PANI·Ag nanohybrid films were similar. The PANI·Ag nanohybrid nanosensor had a limit of detection of less than 3.1 ppq and limit of quantification of 10.05 ppq. The nanosensor layers were analyzed by UV-vis and FTIR showing the incorporation of Ag nanoparticles in the nanohybrid. We found that pheromone compound was adsorbed in sensing layer resulting in a reduction in the resonance frequency. The detection mechanism help us understand the good results of LOD, LOQ, sensitivity, selectivity and repeatability. The presented device has great potential for detection of the sexual pheromone from E. heros.

Correction: Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors.

Correction for 'Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors' by Janine Martinazzo et al., Anal. Methods, 2021, 13, 3900-3908, DOI: 10.1039/d1ay00987g.

Electronic Nose Based on Carbon Nanocomposite Sensors for Clove Essential Oil Detection.

This work describes the development of an electronic nose (e-nose) based on carbon nanocomposites to detect clove essential oil (CEO), eugenol (EUG), and eugenyl acetate (EUG.ACET). Our e-nose system comprises an array of six sensing units modified with nanocomposites of poly(aniline), graphene oxide, and multiwalled carbon nanotubes doped with different acids, dodecyl benzene sulfonic acid, camphorsulfonic acid, and hydrochloric acid. The e-nose presented an excellent analytical performance to the detected analytes (CEO, EUG, and EUG.ACET) with high sensitivity and reversibility. The limit of detection was lower than 1.045 ppb, with response time (<13.26 s) and recovery time (<106.29 s) and low hysteresis. Information visualization methods (PCA and IDMAP) demonstrated that the e-nose was efficient to discriminate the different concentrations of analyte volatile oil compounds. PM-IRRAS measurements suggest that the doping mechanism of molecular architectures is composed of a change in the oscillation energy of the characteristic dipoles and changes in the molecular orientation dipoles C═C and C═O at 1615 and 1740 cm-1, respectively. The experimental results indicate that our e-nose system is promising for a rapid analysis method to monitor the quality of essential oils.

Interfacial and emulsifying properties of whey protein concentrate by ultrafiltration.

The aim of this study was to concentrate whey protein by ultrafiltration process, evaluating the pressure at 1-3 bar and temperature of 10-20℃. In the conditions that show the more protein concentration were evaluated the interfacial and emulsifying properties at pH 5.7 and 7.0. The whey concentrate at 10℃ and 1.5 bar showed the higher protein value 36% (w/w), with soluble protein of 33.82% (solubility of 93.94%) for pH 5.7 and 34% (solubility of 94.4%) for pH 7.0, respectively. The whey concentrate powder present particle size distribution between 0.4-110 um. The whey at pH 5.7 and 7.0 was not observed significant differences in the resistance parameters of the oil/water layer interface. The interfacial film formed by the proteins presented an essentially elastic behavior in both pH, and in pH 5.7 the emulsion was more stable with lower diameter droplets. The concentrate whey showed techno-functional properties (emulsification and solubility), which allow the use as ingredients in products of industrial interest in food products such as mayonnaise, ice cream, sauces, and others.