RESUMO
Simultaneous rotational and vibrational temperatures are measured in an N2 plasma with rotational coherent anti-Stokes Raman scattering (CARS) resolved with a virtually imaged phased array (VIPA)-based spectrometer. A VIPA spectrally separates rotational transitions for each vibrational state, allowing vibrational populations to be directly measured. VIPA-CARS is shown to provide more accurate measurements of non-equilibrium temperatures than grating-resolved rotational CARS. The general characteristics, limitations, and potential uses of VIPA-CARS are discussed.
RESUMO
Coherent anti-Stokes Raman scattering (CARS) is commonly used for thermometry and concentration measurement of major species. The quadratic scaling of CARS signal with number density has limited the use of CARS for detection of minor species, where more sensitive approaches may be more attractive. However, significant advancements in ultrafast CARS approaches have been made over the past two decades, including the development of hybrid CARS demonstrated to yield greatly increased excitation efficiencies. Yet, detailed detection limits of hybrid CARS have not been well established. In this Letter, detection limits for N2, H2, CO, and C2H4 by point-wise hybrid femtosecond (fs)/picosecond (ps) CARS are determined to be of the order of 1015 molecules/cm3. The possible benefit of fs/nanosecond (ns) hybrid CARS is also discussed.
Assuntos
Análise Espectral Raman , TermometriaRESUMO
Coherent anti-Stokes Raman scattering (CARS) is a valuable spectroscopic tool for the measurement of temperature and species concentration. In recent years, multi-dimensional CARS has seen focused development and is especially important in reacting flows. An important aspect of multi-dimensional CARS is the phase-matching scheme used. Historically, collinear and BOXCARS phase-matching schemes have been used to achieve phase matching over a broad spectral range. For 1-D and 2-D CARS imaging, two-beam or counter-propagating beam arrangements are necessary. The two-beam arrangement offers many advantages, but introduces a phase mismatch which limits the spectral response of the measurement. This work explores the tradeoffs in spatial resolution, spectral bandwidth, and CARS intensity in 2-D CARS arrangements. Calculations are made for two-beam and counter-propagating beam CARS.
RESUMO
The Chlamydomonas reinhardtii cell wall is made up of hydroxyproline-rich glycoproteins, arranged in five distinct layers. The W6 (crystalline) layer contains three major glycoproteins (GP1, GP2, GP3), selectively extractable with chaotropic agents, that self-assemble into crystals in vitro. A system to study W6 assembly in a quantitative fashion was developed that employs perchlorate-extracted Chlamydomonas cells as nucleating agents. Wall reconstitution by biotinylated W6 monomers was monitored by FITC-streptavidin fluorescence and quick-freeze/deep-etch electron microscopy. Optimal reconstitution was obtained at monomer concentrations (0.2-0.3 mg/ml) well below those required for nonnucleated assembly. Assembly occurred from multiple nucleation sites, and faithfully reflected the structure of the intact W6 layer. Specificity of nucleated assembly was demonstrated using two cell-wall mutants (cw-2 and cw-15); neither served as a substrate for assembly of wild-type monomers. In addition, W6 sublayers were assembled from purified components: GP2 and GP3 coassembled to form the inner (W6A) sublayer; this then served as a substrate for self-assembly of GP1 into the outer (W6B) sublayer. Finally, evolutionary relationships between C. reinhardtii and two additional members of the Volvocales (Chlamydomonas eugametos and Volvox carteri) were explored by performing interspecific reconstitutions. Hybrid walls were obtained between C. reinhardtii and Volvox but not with C. eugametos, confirming taxonomic assignments based on structural criteria.