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Enteropathies are a serious concern in racing pigeons as they significantly impair performance in races and their training, and viruses are suspected to be one of the main factors. Astroviruses are well-known to be responsible for causing enteric disease in humans and various other animals including birds, although their prevalence and pathogenicity in pigeons is poorly understood. In this study, we investigated 2 groups of young racing pigeons (sick-study group and healthy-control group) to assess the correlation between the number of astrovirus genome copies in cloacal swabs and the occurrence of enteropathy. To determine this, we developed a novel TaqMan quantitative PCR (qPCR) and digital droplet PCR (ddPCR) methods for astrovirus detection and absolute quantitative analysis. We also performed high-throughput sequencing to obtain the complete genome sequences and establish the genetic similarity of the obtained strains to known astroviruses of poultry and other avian species. Two new complete genome sequences of pigeon astroviruses in the Avastrovirus genus were identified, representing 2 new species. These were found most closely related to astroviruses identified in Columbidae species and chickens. They share an average of 75.8% genome-wide pairwise identity and 57.6% and 64.6% capsid protein sequence identity with other unclassified columbid avastrovirus sequences in GenBank. Although the difference in prevalence of astrovirus in the study and control group was found statistically insignificant, there was a significant difference between the number of genome copies in positive samples from both groups. These unambiguous results leave the role of astroviruses as enteropathogenic factors in pigeons still undetermined.
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This study was aimed to investigate the frequency of PiCV recombination, the kinetics of PiCV viremia and shedding and the correlation between viral replication and host immune response in young pigeons subclinically infected with various PiCV variants and kept under conditions mimicking the OLR system. Fifteen racing pigeons originating from five breeding facilities were housed together for six weeks. Blood and cloacal swab samples were collected from birds every seven days to recover complete PiCV genomes and determine PiCV genetic diversity and recombination dynamics, as well as to assess virus shedding rate, level of viremia, expression of selected genes and level of anti-PiCV antibodies. Three hundred and eighty-eight complete PiCV genomes were obtained and thirteen genotypes were distinguished. Twenty-five recombination events were detected. Recombinants emerged during the first three weeks of the experiment which was consistent with the peak level of viremia and viral shedding. A further decrease in viremia and shedding partially corresponded with IFN-γ and MX1 gene expression and antibody dynamics. Considering the role of OLR pigeon rearing system in spreading infectious agents and allowing their recombination, it would be reasonable to reflect on the relevance of pigeon racing from both an animal welfare and epidemiological perspective.
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Doenças das Aves , Infecções por Circoviridae , Circovirus , Columbidae , Eliminação de Partículas Virais , Animais , Columbidae/virologia , Circovirus/genética , Circovirus/imunologia , Infecções por Circoviridae/veterinária , Infecções por Circoviridae/virologia , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/imunologia , Doenças das Aves/virologia , Doenças das Aves/epidemiologia , Doenças das Aves/imunologia , Viremia/epidemiologia , Viremia/virologia , Viremia/imunologia , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Genoma Viral , Recombinação Genética , Genótipo , Replicação Viral , FilogeniaRESUMO
Viruses from Picornaviridae family are known pathogens of poultry, although the information on their occurrence and pathogenicity in pigeons is scarce. In this research, efforts are made to broaden the knowledge on Megrivirus B and Pigeon picornavirus B prevalence, phylogenetic relationship with other avian picornaviruses and their possible connection with enteric disease in racing pigeons. As a result of Oxford Nanopore Sequencing, five Megrivirus and two pigeon picornavirus B-like genome sequences were recovered, among which three recombinant strains were detected. The recombinant fragments represented an average of 10.9% and 25.5% of the genome length of the Pigeon picornavirus B and Megrivirus B reference strains, respectively. The phylogenetic analysis revealed that pigeons are carriers of species-specific picornaviruses. TaqMan qPCR assays revealed 7.8% and 19.0% prevalence of Megrivirus B and 32.2% and 39.7% prevalence of Pigeon picornavirus B in the group of pigeons exhibiting signs of enteropathy and in the group of asymptomatic pigeons, respectively. In turn, digital droplet PCR showed a considerably higher number of genome copies of both viruses in sick than in asymptomatic pigeons. The results of quantitative analysis leave the role of picornaviruses in enteropathies of pigeons unclear.
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Doenças das Aves , Columbidae , Genoma Viral , Filogenia , Infecções por Picornaviridae , Picornaviridae , Animais , Columbidae/virologia , Picornaviridae/genética , Picornaviridae/classificação , Picornaviridae/isolamento & purificação , Doenças das Aves/virologia , Infecções por Picornaviridae/veterinária , Infecções por Picornaviridae/virologia , Recombinação GenéticaRESUMO
Introduction: Alternative protein sources have recently been attracting growing interest as potential components of livestock nutrition. This study evaluated the effect on broiler health of replacing the soybean protein component of poultry feed with processed insect protein from farmed Hermetia illucens (HI) larvae. Material and Methods: A total of 384 male broiler chicks were divided into four diet groups (eight pens/treatment and 12 birds/pen) and reared to the 42nd day of life (dol). Each treatment group received a starter diet until the 14th dol, then a grower diet until the 35th and finally a finisher diet until the 42nd. The soybean meal in standard diets was replaced with full-fat HI larvae meal in the following amounts: 0% for the control group HI-0, 50% for group HI-50, 75% for group HI-75, and 100% for group HI-100. At 1 dol, chicks were vaccinated against Marek's disease, coccidiosis, Newcastle disease and infectious bronchitis and at 7 dol against avian metapneumovirus infections using live-attenuated vaccines. Blood and spleen samples were collected at three and six weeks of age and analysed using ELISA, flow cytometry, haematology and biochemistry. Results: On the 42nd dol, as the content of larvae meal in the chickens' diets increased, the birds' body weights decreased significantly. The substitution of the protein source had no effect on the haematological markers. In chicks that received larvae meal, there was a decrease in creatine kinase activity and phosphorus levels and an increase in calcium and uric acid levels in serum. Raising the proportion of full-fat HI larvae meal in the diet raised the percentage of T CD3+CD8a+ cells and lowered that of T CD3+CD4+ cells in both sample types. Chickens fed larvae meal had significantly lower post-vaccination anti-infectious bronchitis virus antibody titres. Conclusion: The poorer production results and impaired health in experimental birds may indicate lower than 50% protein substitution with full-fat HI larvae meal to be optimal.
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While disease control in racing pigeons and the potential role of pigeons as vectors transmitting viruses to poultry are of importance, there is still a paucity of data concerning the occurrence of coronaviruses in pigeons. In this study, 215 domestic pigeons were tested for the presence of coronaviral genetic material using the nested PCR method, which revealed 57 positive samples (26.51%). The difference in coronavirus prevalence between young and adult pigeons (34.34% and 19.83%, respectively) has been found statistically significant. In contrast, no statistically significant difference has been demonstrated between the prevalence in symptomatic and asymptomatic birds, leaving the influence of coronavirus presence on pigeon health uncertain. Phylogenetic analysis of the RdRp gene fragment allowed us to assign all the obtained strains to the Gammacoronavirus genus and Igacovirus subgenus. The phylogenetic tree plotted using the ML method revealed that those sequences formed a group most similar to pigeon coronavirus strains from China, Finland, and Poland, and to a single strain from a common starling from Poland, which suggests wide geographical distribution of the virus and its possible transmission between various species.
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BACKGROUND: The aim of the study was to determine whether free-living birds belonging to game species whose meat is used for human consumption can constitute a reservoir of pathogenic Campylobacter strains, spreading these bacteria to other hosts or directly contributing to human infection. METHODS: A total of 91 cloacal swabs were taken from different species of wildlife waterfowl to estimate the Campylobacter prevalence, the genetic diversity of the isolates, and the presence of virulence genes and to evaluate the antimicrobial resistance. RESULTS: The presence of Campylobacter spp. was confirmed in 32.9% of samples. Based on flaA-SVR sequencing, a total of 19 different alleles among the tested Campylobacter isolates were revealed. The virulence genes involved in adhesion were detected at high frequencies among Campylobacter isolates regardless of the host species. The highest resistance was observed for ciprofloxacin. The resistance rates to erythromycin and tetracycline were observed at the same level. CONCLUSIONS: These results suggest that wildlife waterfowl belonging to game species may constitute a reservoir of Campylobacter, spreading these bacteria to other hosts or directly contributing to human disease. The high distribution of virulence-associated genes among wildlife waterfowl Campylobacter isolates make them potentially able to induce infection in humans.
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Mycoplasma infections have been found in different species of waterfowl worldwide. However, the question of how the pathogens have been transmitted and dispersed is still poorly understood. Samples collected from clinically healthy greater white-fronted geese (Anser albifrons) (N = 12), graylag geese (Anser anser) (N = 6), taiga bean geese (Anser fabalis) (N = 10), and barnacle geese (Branta leucopsis) (N = 1) were tested for Mycoplasma spp. All Mycoplasma-positive samples were specified by species-specific PCR for Mycoplasma anserisalpingitidis (formerly known as Mycoplasma sp. 1220), M. anseris, M. anatis, and M. cloacale. The presence of Mycoplasma spp. was confirmed in 22 of 29 sampled birds (75.9%). Mycoplasma anserisalpingitidis was the most frequently detected species (15 of 22, 68.2%). However, we did not detect any of the other Mycoplasma spp. typical for geese, among which are M. anatis, M. anseris, and M. cloacale. Phylogenetic analysis revealed that Polish sequences of M. anserisalpingitidis formed a distinct branch, along with 2 Hungarian isolates obtained from domestic geese. Eight of the samples identified as Mycoplasma spp.-positive were negative for the aforementioned Mycoplasma species. A phylogenetic tree constructed based on partial 16S rRNA gene analysis showed that Mycoplasma spp. sequences collected from Polish wild geese represent a distinct phylogenetic group with Mycoplasma sp. strain 2445 isolated from a domestic goose from Austria. The results of our study showed that wild geese could be a reservoir and vector of different species of the Mycoplasma genus that can cause significant economic losses in the domestic goose industry.
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Gansos , Mycoplasma , Animais , Galinhas , Mycoplasma/genética , Filogenia , RNA Ribossômico 16SRESUMO
The dynamic development of flexible wearable electronics creates new possibilities for the production and use of new types of sensors. Recently, polymer nanocomposites have gained great popularity in the fabrication of sensors. They possess both the mechanical advantages of polymers and the functional properties of nanomaterials. The main drawback of such systems is the complexity of their manufacturing. This article presents, for the first time, fabrication of an antimony sulfoiodide (SbSI) and polyurethane (PU) nanocomposite and its application as a piezoelectric nanogenerator for strain detection. The SbSI/PU nanocomposite was prepared using simple, fast, and efficient technology. It allowed the obtainment of a high amount of material without the need to apply complex chemical methods or material processing. The SbSI/PU nanocomposite exhibited high flexibility and durability. The microstructure and chemical composition of the prepared material were investigated using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. These studies revealed a lack of defects in the material structure and relatively low agglomeration of nanowires. The piezoelectric response of SbSI/PU nanocomposite was measured by pressing the sample with a pneumatic actuator at different excitation frequencies. It is proposed that the developed nanocomposite can be introduced into the shoe sole in order to harvest energy from human body movement.
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Nanocompostos , Poliuretanos , Humanos , Poliuretanos/química , Microscopia Eletrônica de Varredura , Nanocompostos/química , Fenômenos FísicosRESUMO
Animal production is identified as one of the main sources of high concentrations of odours, which are related to air pollution, health problems of living organisms and indirect negative impact on production results. One common method for reducing emissions of ammonia is using preparations containing probiotics and hygroscopic or disinfecting compounds. This study was undertaken in order to determine the impact of innovative mineral-microbial deodorizing preparation, which reduces odorous gases, applying to the litter once a week in poultry houses on the physiological status of breeder chickens, broiler chickens and turkeys. Samples were collected after slaughter and analyzed using ELISA tests, flow cytometry and biochemical methods. Biochemical markers of the liver and kidney profile (ALT, AST, LDH, ALP, CK, TP, CALC, PHOS) and the titers of specific antibodies against AEV, aMPV, AAvV-1, IBDV, HEV, BA were analyzed in serum samples. The percentage contribution of T and B lymphocyte subpopulations was determined in the samples of tracheal mucosa, blood, and spleen. No significant differences were found between the control and experimental group with regard to all the analyzed parameters, with some exceptions for biochemistry. The results of our study indicated that mineral-microbial deodorizing preparation did not affect the physiological status of birds.
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Pigeon circovirus (PiCV) infects pigeon populations worldwide and has been associated with immunosuppression in younger pigeons. Recombination is a common mechanism of evolution that has previously been shown in various members of the Circoviridae family, including PiCV. In this study, three groups of pigeons acquired from separate lofts were screened for PiCV, and their genome sequence was determined. Following this, they were housed in a single loft for 22 days, during which blood and cloacal swab samples were taken. From these blood and cloacal swabs, PiCV genomes were determined with the aim to study the spread and recombination dynamics of PiCV in the birds. Genome sequences of PiCV were determined from seven pigeons (seven tested PiCV positive) before they were housed together in a loft (n = 58 sequences) and thereafter from the ten pigeons from blood and cloacal swabs (n = 120). These 178 PiCV genome sequences represent seven genotypes (98% pairwise identity genotype demarcation), and they share >88% genome-wide pairwise identity. Recombination analysis revealed 13 recombination events, and a recombination hotspot spanning the 3' prime region, the replication-associated protein (rep) gene and the intergenic region. A cold spot in the capsid protein-coding region of the genome was also identified. The majority of the recombinant regions were identified in the rep coding region. This study provides insights into the evolutionary dynamics of PiCV in pigeons kept under closed rearing systems.
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Doenças das Aves/virologia , Infecções por Circoviridae/veterinária , Circovirus/genética , Columbidae/virologia , Recombinação Genética , Animais , Animais Domésticos , Biologia Computacional/métodos , DNA Viral , Genoma Viral , Genômica/métodos , Genótipo , Filogenia , Projetos PilotoRESUMO
INTRODUCTION: Turkey histomonosis poses a serious threat to poultry production due to the ban on the use of effective drugs. The aim of the study was to evaluate the influence of a phytoncidal feed supplement on the course of histomonosis. The preparation was also analysed for immunomodulatory properties. MATERIAL AND METHODS: Clinical observations and production monitoring were conducted in a flock of turkeys with histomonosis from their 11th to 56th weeks of life which were treated with the adiCoxSOLPF soluble supplement in a dose of 2.5 mL/L water. Later the preparation was used in a preventive dose (1 mL/L). The influence on the immune system was evaluated in broiler turkeys having been given adiCoxSOLPF for 3 days in doses of 1 or 3 mL/L. The T and B lymphocyte percentages in turkey blood and spleen tissue were analysed with flow cytometry. ELISA was implemented to evaluate antibody titres after Ornithobacterium rhinotracheale vaccination, and biochemical analyses were performed to evaluate the supplement's safety. RESULTS: AdiCoxSOLPF was found effective in therapy and prevention of histomonosis. Additionally, adiCoxSOLPF stimulated both humoral and cell-mediated immune mechanisms, without impairing the functions of internal organs. The treated turkeys also yielded better production results (eggs/hen, fertility, and hatchability). CONCLUSION: AdiCoxSOLPF possesses immunomodulatory properties and it can be used successfully in the prevention and therapy of histomonosis in turkeys.
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The complete genome sequence of a bacteriophage in the genus Phapecoctavirus (family Myoviridae) isolated from a cloacal swab specimen from a domestic pigeon (Columba livia f. domestica) was identified using a high-throughput sequencing approach. The genome is 150,892 bp with a GC content of 39.1%, containing 269 open reading frames and 11 tRNA genes.
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Salmonellosis is one of the most important bacterial diseases in pigeons. This study aimed to estimate the prevalence of Salmonella spp. in domestic pigeons (Columba livia f. domestica) in Poland, its antimicrobial susceptibility (both phenotypic and genotypic), and its capability for biofilm formation. The presence of selected virulence genes, nucleotide homology of selected genes, and susceptibility to bacteriophages were investigated as well. From the 585 pigeons tested, 5.47% turned out positive. All isolated strains were recognized as Salmonella enterica ser. Typhimurium. The asymptomatic pigeons were carriers of 37.5% of the isolates. The dominant variants were as follows: 1,4,[5],12,:i:1,2 (53.13%) and 1,4,[5],12,:-:- (31.25%). Most of the strains analysed showed the ability to produce biofilm after 24 and 48 hr of incubation (59.38% and 53.13%, respectively). Over 90% of the strains were confirmed for lpfA, agafA, invA, sivH, and avrA virulence genes. Also, of the thirteen antimicrobial susceptibility genes, the following were confirmed: sul1, tet(A), blaTEM-1 , floR, strA, and strB. The most common were the strB (18%) and tet(A) (12%) genes that are responsible for coding resistance to aminoglycosides and tetracyclines, respectively. Most of the strains were phenotypically resistant to oxytetracycline (46.88%), neomycin (53.13%) and tylosin (100%). The susceptibility of the investigated Salmonella strains to the bacteriophages was between 33% and 100%. MLST, PCR MP and ERIC PCR analyses indicated a very high genetic similarity of the investigated strains (over 99%). Results of our study indicate that Salmonella enterica ser. Typhimurium is still an important agent in domestic pigeons and that its antimicrobial resistance increases. Alarming is also the confirmation of a single-phase variant 1,4,[5],12:i,-, which could have increased virulence and multi-drug resistance encoded on the plasmid. Most importantly, however, such strains have been isolated from humans with clinical symptoms of Salmonella infection.
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Columbidae , Salmonella enterica , Animais , Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Humanos , Testes de Sensibilidade Microbiana/veterinária , Tipagem de Sequências Multilocus/veterinária , Salmonella/efeitos dos fármacos , Salmonella/genética , Salmonella enterica/genéticaRESUMO
The diseases with watery droppings (diarrhea and/or polyuria) can be considered some of the most severe health problems in domestic pigeons of various ages. Although they do not always lead to bird death, they can contribute to poor weight gains and hindered development of young pigeons and, potentially, to poor racing results in sports birds. The gastrointestinal tract disorders of pigeons may be of various etiology, but some of the causative agents are viral infections. This review article provides information collected from scientific reports on RNA-viruses belonging to the Astroviridae, Picornaviridae, and Coronaviridae families; the Avulavirinae subfamily; and the Rotavirus genus that might be implicated in such health problems. It presents a brief characterization, and possible interspecies transmission of these viruses. We believe that this review article will help clinical signs of infection, isolation methods, occurrence in pigeons and poultry, systemize and summarize knowledge on pigeon enteropathogenic viruses and raise awareness of the importance of disease control in pigeons.
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The pathology of pigeon circovirus (PiCV) is still unknown, but it is regarded as an immunosuppressant. This study aimed to find a correlation between PiCV natural infection and immunosuppression. The study was conducted with 56 pigeons divided into the following groups: PiCV-positive but showing (group S) or not (group I) non-specific clinical symptoms and asymptomatic pigeons negative for PiCV (group H). The percentage and apoptosis of T CD3+ and B IgM+ splenocytes; the expression of CD4, CD8, and IFN-γ genes in splenic mononuclear cells; the number of PiCV viral loads in the bursa of Fabricius; and the level of anti-PiCV antibodies were analyzed. The results showed that the percentage of B IgM+ cells was almost two-fold lower in group S than in group H, and that ca. 20% of the lymphocytes were apoptotic. No increased apoptosis was detected in TCD3+ subpopulation. The PiCV viral loads were approximately one thousand and ten thousand times higher in group S than in groups I and H, respectively. Our results indicate a possible correlation between the number of PiCV viral loads and severity of PiCV infection and confirm that PiCV infection leads to the suppression of humoral immunity by inducing B lymphocyte apoptosis.
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Considering the lack of research on the local immunity of the reproductive tract in other poultry species than chickens, the aim of this study was to determine the contribution of T and B cell subpopulations in different parts of breeder turkey oviduct mucous membrane with the use of flow cytometry. In addition, the study aimed to establish an impact of bird age and different stages of the egg production cycle on the systemic and local oviduct-related immune system structure. Our study results demonstrated a lower percentage of T lymphocytes in 32-wk turkey hens followed by a successively increasing population of these cells up to the 38th week of bird's life. The results of our study have also shown a similar dependency between birds' age and number of B lymphocytes. In addition, we demonstrated a decrease in the number of immune system cells in the oviduct, blood, and spleen of turkey hens in the late and end laying period. The differences reported in the number of lymphocyte subpopulations in the reproductive system of laying turkey hens at various stages of the production cycle may, to some extent, explain the frequency and periods of increased predilection to the incidence of infectious diseases in birds under field conditions.
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Linfócitos B/metabolismo , Oviductos/metabolismo , Linfócitos T/metabolismo , Perus/fisiologia , Fatores Etários , Animais , Feminino , Mucosa/fisiologiaRESUMO
The risk of meat contamination with Yersinia enterocolitica poses a threat to consumers and persons who come into contact with bird carcasses. The occurrence of Y. enterocolitica in the vast majority of migratory game species, the capercaillie, and the black grouse has never been studied in Poland, Europe, or in the world. The material for the study consisted of cloacal swabs obtained from 143 Eurasian coots, 50 mallards, 30 pochards, 27 greylag geese, 22 white-fronted geese, 22 bean geese, 20 green-winged teals, and 10 tufted ducks, as well as fecal swabs obtained from 105 capercaillie and 18 black grouse. Bacteriological examinations of 894 samples taken from 447 birds led to the isolation of 20 strains with the biochemical features characteristic of the genus Yersinia. All 20 strains were molecularly examined, and the genes characteristic of Y. enterocolitica were detected in 8 strains. The isolated strains harbored amplicons whose size corresponded to ystB gene fragments. Four strains belonged to bioserotype 1A/NI, one strain was identified as bioserotype 1B/O:9, and one as 1A/O:9. The prevalence of Y. enterocolitica was determined at 1.4% in green-winged teals, at 5.0% in Eurasian coots, and at 4.8% in capercaillie. All strains were resistant to amoxicillin with clavulanic acid, ampicillin, and cefalexin. The strains isolated from migratory birds were also resistant to kanamycin and streptomycin, and they were characterized by resistance or intermediate resistance to cefotaxime, ceftazidime, chloramphenicol, gentamycin, and tetracycline, to which the strains isolated from the capercaillie were susceptible. Yersinia enterocolitica was not detected in the remaining bird species. The presence of Y. enterocolitica in green-winged teals, Eurasian coots, and capercaillie indicates that these birds could be carriers, potential reservoirs, and sources of infection for humans. They can also be regarded as reliable bioindicators of Y. enterocolitica in their respective habitats.
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Aves/microbiologia , Sorogrupo , Fatores de Virulência/genética , Yersinia enterocolitica , Animais , Humanos , Polônia , Yersinia enterocolitica/genética , Yersinia enterocolitica/isolamento & purificação , Yersinia enterocolitica/patogenicidadeRESUMO
Infections with immunosuppressive pigeon circovirus (PiCV) pose the most severe health problem to the global pigeon breeding. The vaccination with immunogenic PiCV recombinant capsid protein (PiCV rCP) is a potential tool for disease control. Because of the high prevalence of PiCV asymptomatic infections, the subclinically infected pigeons will be vaccinated in practice. The aim of this study was to answer a question if vaccination of asymptomatic, infected with PiCV pigeons induces a similar immune response to PiCV rCP as in uninfected birds. One hundred and twenty 6-week-old carrier pigeons were divided into 4 groups (2 groups of naturally infected and uninfected with PiCV individuals). Birds from groups V and V1 were vaccinated twice with PiCV rCP mixed with an adjuvant, whereas pigeons from groups C and C1 were immunized with an adjuvant only. The expression of genes encoding IFN-γ, CD4, and CD8 T lymphocyte receptors; the number of anti-PiCV rCP IgY-secreting B cells (SBC) and anti-PiCV rCP IgY were evaluated 2, 21, 39 and 46 days post vaccination (dpv). Study results showed that the expression of CD8 and IFN-γ genes was higher in both groups of infected pigeons than in the uninfected birds, irrespective of vaccination. In the uninfected birds, the expression of these genes was insignificantly higher in the vaccinated pigeons. The anti-PiCV rCP IgY-SBC were detected on 2 and 23 dpv and seroconversion was noted on 23 and 39 dpv in V and V1 groups, respectively. In the light of the results obtained, it could be concluded that pigeon circovirus recombinant capsid protein elicits the immune response in both naturally infected and uninfected pigeons, but its rate varies depending on PiCV infectious status. The infection with PiCV masks the potential cellular immune response to the vaccination with PiCV rCP and leads to the suppression of humoral immunity.
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Doenças das Aves/tratamento farmacológico , Proteínas do Capsídeo/administração & dosagem , Infecções por Circoviridae/veterinária , Circovirus/metabolismo , Vacinas Virais/administração & dosagem , Animais , Anticorpos Antivirais/metabolismo , Doenças das Aves/imunologia , Antígenos CD8/genética , Proteínas do Capsídeo/imunologia , Infecções por Circoviridae/tratamento farmacológico , Infecções por Circoviridae/imunologia , Circovirus/imunologia , Columbidae , Interferon gama/genética , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Resultado do Tratamento , Regulação para Cima , Vacinação/veterinária , Vacinas Virais/imunologiaRESUMO
Immunoglobulins, which are passed vertically from hens to their progeny, are first present in the eggs but with time also in the developing embryos and eventually in the serum of hatching chicks, and have protective function during embryogenesis and in the first few weeks of birds' life, before the immune system becomes fully efficient. Considering the above fact, the aim of this study was to determine total levels of IgM and IgY as well as specific IgY antibody titers against selected pathogens in the serum of breeder turkeys and their progeny, as well as in egg yolks and egg whites. Study results demonstrated that the level of IgY antibodies in the serum of turkey breeder hens reached 22.04 mg/mL on average in the whole egg laying cycle. In addition, the mean transfer percentage of IgY antibodies from turkey layers to their progeny reached approximately 31.4%, but the level of this transfer differed depending on pathogen character and accounted for 33.2%, 51.9%, 45.1%, and 44.3% in the case of antibodies against avian metapneumoviruses, Newcastle disease virus, Ornithobacterium rhinortacheale, and Pasteurella multocida, respectively. Antibody percentage transfer differed also as affected by the stage of the egg production cycle. Study results confirmed the earlier observed dependency concerning the class of antibodies transferred to eggs from laying hens, and while the IgY were mainly detected in the egg yolk extracts, the IgM were found only in egg white extracts; in comparison to IgY, the IgM antibodies were not transferred to the serum of turkey poults. To our best knowledge, this is the first study that describes in detail the phenomenon of maternal antibody transfer in turkeys.
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Anticorpos/análise , Imunidade Materno-Adquirida , Óvulo/imunologia , Doenças das Aves Domésticas/imunologia , Animais , Feminino , Imunoglobulina G/sangue , Imunoglobulinas/sangue , Metapneumovirus/imunologia , Vírus da Doença de Newcastle/imunologia , Ornithobacterium/imunologia , Pasteurella multocida/imunologia , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/virologia , Perus/imunologiaRESUMO
This paper is the first record describing the molecular analysis of Eimeria species occurring in capercaillie (Tetrao urogallus) and black grouse (Tetrao tetrix) which inhabit northern Eurasia and are species critically endangered of extinction. Actions undertaken to protect endangered species, such as breeding individuals in closed aviaries, could allow saving those birds, but they also pose risk of accidental healing of invasive diseases, like coccidiosis. Therefore, an investigation was conducted on fecal samples collected from the capercaillies and black grouse originating from the Kirov region (Russia) and breeding centers located in Poland. Results indicate that the average prevalence of Eimeria revealed 72% (average OPG = 3548) and 80% (average OPG = 5220) in capercaillies and black grouse respectively. Most of the Eimeria spp. oocysts were non-sporulated; however, two different morphological types were observed. The phylogenetic analysis of cox-1 and 18S rRNA genes revealed the analyzed Eimeria sequences to belong to two species. In addition, it showed some similarities between both analyzed genes. Most of the sequences obtained from both grouse species coccidia belonged to one species partially homologous to the Eimeria spp. isolated from ring-necked pheasant (approx. 94 and 96% for cox-1 and 18S rRNA genes, respectively). Two strains isolated from capercaillies imported from Russia were related to turkey coccidia: E. innocua and E. dispersa (97-99% homology) in the cox-1 gene analysis and only one of them was related to those Eimeria species in the 18S rRNA gene analysis (98-99% homology).