Biochemical and X-ray micro-computed tomographic analyses of critical size bone defects grafted with autogenous bone and mercerized bacterial cellulose membranes salified with alendronate.

OBJECTIVES: This study aimed to evaluate the repair of critical-sized bone defects grafted with autogenous bone and mercerized bacterial cellulose membranes (BCm) salified with alendronate (ALN). METHODS: Forty-eight male Wistar rats underwent surgery to create a 5 mm-diameter bone defect in the calvarium. The removed bone was particularized, regrafted into the defect, and covered by a BCm according to the group: control group (CG), simply mercerized BCm; group 1 (G1), negatively charged BCm (BCm-CM-) salified with ALN; and group 2 (G2), positively charged BCm (BCm-DEAE+) salified with ALN. Serum samples were collected preoperatively and before euthanasia to analyze osteoprotegerin (OPG), parathyroid hormone (PTH), sclerostin (SOST), and fibroblast growth factor 23 (FGF23) levels. The animals were euthanized after 15 or 60 d. Calvaria were analyzed using quantitative microtomography (µCT). RESULTS: There was an increased level of PTH in the CG compared to the G2 group, at day 60 (p = 0.019). When analyzing the same group over time, G1 presented an increased FGF23 level on days 15 and 60 (p < 0.05). CG presented an increase in PTH (p = 0.037) at day 60. The µCT analysis detected increased trabecular separation on day 15 in G2 compared to G1 (p = 0.040). CONCLUSIONS: Salification of ionized BCm with ALN had no direct effect on bone repair; however, BCm-CM- increased the levels of FGF23 over time. BCm-DEAE+ decreased PTH levels compared to mercerized BCm. BCm-CM-salified with ALN-induced superior bone quality, with respect to trabecular separation, compared to BCm-DEAE+.

Salivary pH and oral health of Brazilian para-athletes: Saliva and oral health of para-athletes.

AIMS: This study aimed to investigate the association of the salivary pH and parameters of oral health in Brazilian para-athletes. METHODS AND RESULTS: The sample was composed of 271 Brazilian para-athletes (147 athletics, 61 powerlifting, and 63 swimming); mean age was 31.2 ± 11.7 years. Data collected during anamnesis, clinical examination, and saliva collection revealed that salivary pH was different among sports (p = .01) and tends to decrease as age and body mass index (BMI) increase (p = .026, .027, respectively). The mean decay missing filled teeth was 8.8 (±0.57), but there is no correlation with salivary pH (R2  = -0.0852; CI 95%, -0.215-0.047; p = .194) as habits of grinding, clenching teeth, or bruxism was not associated with salivary pH (p = .317, .932, and .444, respectively). Regarding breathing, para-athletes that have buccal breathing had significantly higher salivary pH (p = .04). This data were confirmed by multiple logistic regression (p = .05). Open bite, lip seal, geographic or fissured tongue also were not associated with variation of salivary pH (p > .05). CONCLUSIONS: This study provided evidence of an association between salivary pH with age, BMI, type of breathing, and type of sport practiced by the para-athletes.