Are inter-limb differences in change of direction velocity and angle associated with inter-limb differences in kinematics and kinetics following anterior cruciate ligament reconstruction?

BACKGROUND: Quantifying inter-limb differences in kinematics and kinetics during change of direction is proposed as a means of monitoring rehabilitation following anterior cruciate ligament reconstruction (ACLR). Velocity and centre of mass (CoM) deflection angle are fundamental task descriptors that influence kinematics and kinetics during change of direction. Inter-limb differences in approach velocity and CoM deflection angle have been identified following ACLR and may contribute to the presence of inter-limb differences in kinematics and kinetics during change of direction. RESEARCH QUESTION: The aim of this study was to quantify the proportion of variance in kinematic and kinetic inter-limb differences attributable to inter-limb differences in approach velocity and centre of mass deflection angle during a change of direction task. METHODS: A cohort of 192 patients (male, 23.8 ± 3.6 years, 6.3 ± 0.4 months post primary ACLR) completed a pre-planned 90° change of direction task on both their operated and non-operated limb. Inter-limb differences in approach velocity and CoM deflection angle were calculated alongside lower-extremity kinematic and kinetic variables. The relationship between inter-limb differences in task-level variables and inter-limb differences in kinematic and kinetic variables was examined using linear regression models. Kinematic and kinetic inter-limb differences were adjusted for inter-limb differences in approach velocity and CoM deflection angle. Adjusted and unadjusted inter-limb differences were submitted to one sample t-tests. RESULTS: Inter-limb differences in approach velocity and centre of mass deflection angle explained 3 - 60% of the variance in kinematic and kinetic inter-limb differences. Statistical inferences remained consistent between adjusted and unadjusted conditions with the exception of hip flexion angle. SIGNIFICANCE: Inter-limb differences in task-level features explain a large proportion of the variance in inter-limb differences in several kinematic and kinetic variables. Accounting for this variation reduced the magnitude of kinematic and kinetic inter-limb differences comparable to those previously observed in normative cohorts.

Training program intervention to encourage physical activity for health in people with transtibial amputation: A feasibility study.

BACKGROUND: People with transtibial amputation (PTTA) would benefit from increased physical activity levels (PAL) but generic programs developed to support increased PAL do not address the barriers which PTTA experience. OBJECTIVE: To evaluate the effect of a 12-week training program, developed for PTTA, on their PAL. METHODS: Ten PTTA participated in a 12-week training program, which involved one instructor-led supervised group session per week. The program incorporated balance, flexibility, cardiovascular endurance, strength, and agility, as well as educational elements. Personalized starting level and progression intensity were based on the instructor's assessment in the first training session. The effect of the intervention on PAL (self-report questionnaire and accelerometer), fitness, walking symmetry, and adverse effects was determined by analysis of variance before and after the intervention. RESULTS: There was a significant increase in self-reported time in moderate-intensity activity and accelerometer-measured step count and time in movement after the program compared with baseline ( p = 0.02). The distance walked in the Six-Minute Walk Test (6MWT) ( p < 0.001), time to perform agility test ( p = 0.01), and lower-limb strength power ( p = 0.01) and endurance ( p = 0.01) were significantly greater after the program, and no adverse effects were identified. CONCLUSIONS: This study demonstrated an intervention designed specifically for PTTA that can increase PAL.

Altered mechanics and increased loading on intact limbs of individuals with a unilateral transtibial amputation in comparison with non-amputees during a start-stop task.

Individuals with a unilateral transtibial amputation (ITTA) often experience greater loading on the intact limb during running and stepping tasks compared to individuals without amputation. This study aimed to investigate the mechanics of load absorption in the intact limb of ITTA and determine if increased ground reaction forces (GRF) persist during a start-stop task which (i) controlled touch-down velocity and (ii) removed the requirement for on-going locomotion. Data were collected using a twelve-camera motion capture system with two Kistler force platforms. Variables were extracted during the final loading phase of a 2-step start-stop task. The intact limb of ITTA and the dominant limb of able-bodied controls were compared using independent t-tests and effect size analysis. ITTA showed lower knee flexion angles at touchdown (p = 0.007, g = -1.43), and peak vertical GRF (p = 0.01, g = -1.33) compared to control subjects. ITTA also exhibited less hip (p = 0.14, g = 0.76) and ankle (p = 0.002, g = 1.82) absorptive power at touchdown and at peak vertical GRF (hip: p = 0.01, g = 1.23; ankle: p = 0.05, g = 0.97). ITTA exhibited greater peak vertical GRF (p = 0.01, g = 1.30) and braking GRF (p = 0.05, g = -0.96) on the intact limb compared to the controls. Our results indicate altered joint mechanics through the intact limb of ITTA are independent of the touchdown conditions or the need for ongoing locomotion. These altered joint mechanics increased loading experienced by the intact limb. Further work should be conducted examining a variety of other dynamic movements to fully understand the involved mechanics, so that intervention studies can be developed to reduce the load experienced by ITTA.

Six methods for classifying lower-limb dominance are not associated with asymmetries during a change of direction task.

Quantifying asymmetries between dominant and non-dominant limbs is a common research objective aimed at identifying systematic differences between limbs and establishing normative ranges of asymmetry. Multiple methods for classifying limb dominance exist, and it is unclear how different methods relate to directional asymmetries during change of direction (CoD). This study aimed to determine whether different methods of classifying limb dominance, including a novel CoD task-specific method, identified significant inter-limb asymmetries during a 90° CoD task. Fifty participants completed a testing battery consisting of jumping, hopping, CoD, and isokinetic dynamometry. Limb dominance was classified for each participant according to preferred kicking limb, vertical jump height, horizontal hop distance, initial force plate contact during landing, max isokinetic knee extensor strength, and turning velocity. Asymmetries in whole-body and joint-level mechanics were defined using each method. No method for classifying limb dominance was associated with consistent inter-limb biomechanical asymmetries during CoD, and no method was related to any other method. The magnitude of asymmetry relative to the magnitude of absolute asymmetry present within the cohort suggests that using these tasks to classify the dominant limb in this CoD is akin to assigning dominance to a randomly selected limb. Previous observations of group symmetry during CoD may be statistical artifacts as opposed to a true indication of normative movement. Until an appropriate means of classifying limbs during CoD is established, quantifying normative asymmetry based on limb dominance should be done with caution.

The associations between asymmetries in quadriceps strength and gait in individuals with unilateral transtibial amputation.

BACKGROUND: Individuals with unilateral transtibial amputations (ITTAs) are asymmetrical in quadriceps strength. It is unknown if this is associated with gait performance characteristics such as walking speed and limb symmetry. RESEARCH QUESTION: Are quadriceps strength asymmetries related to walking speed and/ or gait asymmetries in ITTAs? METHODS: Knee-extensor isometric maximum voluntary torque (MVT) and rate of torque development (RTD) were measured in eight ITTAs. Gait data were captured as the ITTAs walked at self-selected habitual and fast speeds. Step length and single support time, peak knee extension moments and their impulse and peak vertical ground reaction force (vGRF) in the braking and propulsive phases of stance were extracted. Bilateral Asymmetry Index (BAI) and, for gait variables only, difference in BAI between walking speeds (ΔBAI) were calculated. Correlation analyses assessed the relationships between MVT and RTD asymmetry and (1) walking speed; (2) gait asymmetries. RESULTS: Associations between strength and gait BAIs generally became more apparent at faster walking speeds, and when the difference in BAI between fast and habitual walking speed was considered. BAI RTD was strongly negatively correlated with habitual and fast walking speeds (r=∼0.83). Larger BAI RTD was strongly correlated with propulsive vGRF BAI in fast walking, and larger ΔBAIs in vGRF during both the braking and propulsion phases of gait (r = 0.74-0.92). ITTAs who exhibited greater BAI MVT showed greater ΔBAI in single support time (r = 0.83). SIGNIFICANCE: While MVT and RTD BAI appear to be associated with gait asymmetries in ITTAs, the magnitude of the asymmetry in RTD appears to be a more sensitive marker of walking speed. Based on these results, it's possible that strengthening the knee-extensors of the amputated limb to improve both MVT and RTD symmetry may benefit walking speed, and reduce asymmetrical loading in gait.

Biomechanical but Not Strength or Performance Measures Differentiate Male Athletes Who Experience ACL Reinjury on Return to Level 1 Sports.

BACKGROUND: Performance measures such as strength, jump height/length, and change of direction (CoD) time during anterior cruciate ligament (ACL) rehabilitation have been used to determine readiness to return to play and identify those who may be at risk of rerupture. However, athletes may reach these criteria despite ongoing biomechanical deficits when performing these tests. Combining return-to-play criteria with an assessment of movement through 3-dimensional (3D) biomechanics in male field sports athletes to identify risk factors for ACL rerupture has not been explored previously. PURPOSE: To prospectively examine differences in strength, jump, and CoD performance and movement using 3D biomechanics in a cohort of male athletes playing level 1 sports (ie, multidirectional field sports that involve landing, pivoting, or CoD) between those who reinjured the reconstructed ACL (RI group) and those with no reinjury (NRI group) after 2 years of follow-up and to examine the ability of these differences to predict reinjury. STUDY DESIGN: Cohort study; Level of evidence, 2. METHODS: After primary ACL reconstruction (ACLR), 1045 male athletes were recruited and underwent testing 9 months after surgery including isokinetic strength, jump, and CoD performance measures as well as patient-reported outcomes and 3D biomechanical analyses. Participants were followed up after 2 years regarding ACL reinjury status. Differences were determined between the RI and NRI groups in patient-reported outcomes, performance measures, and 3D biomechanics on the ACLR side and symmetry between limbs. The ability of these measures to predict ACL reinjury was determined through logistic regression. RESULTS: No differences were identified in strength and performance measures on the ACLR side or in symmetry. Biomechanical analysis indicated differences on the ACLR side primarily in the sagittal plane for the double-leg drop jump (effect size, 0.59-0.64) and greater asymmetry primarily in the frontal plane during unplanned CoD (effect size, 0.61-0.69) in the RI group. While these biomechanical test results were different between groups, multivariate regression modeling demonstrated limited ability (area under the curve, 0.67 and 0.75, respectively) to prospectively predict ACL reinjury. CONCLUSION: Commonly reported return-to-play strength, jump, and timed CoD performance measures did not differ between the RI and NRI groups. Differences in movement based on biomechanical measures during double-leg drop jump and unplanned CoD were identified, although they had limited ability to predict reinjury. Targeting these variables during rehabilitation may reduce reinjury risk in male athletes returning to level 1 sports after ACLR. REGISTRATION: NCT02771548 (ClinicalTrials.gov identifier).

Can Biomechanical Testing After Anterior Cruciate Ligament Reconstruction Identify Athletes at Risk for Subsequent ACL Injury to the Contralateral Uninjured Limb?

BACKGROUND: Athletes are twice as likely to rupture the anterior cruciate ligament (ACL) on their healthy contralateral knee than the reconstructed graft after ACL reconstruction (ACLR). Although physical testing is commonly used after ACLR to assess injury risk to the operated knee, strength, jump, and change-of-direction performance and biomechanical measures have not been examined in those who go on to experience a contralateral ACL injury, to identify factors that may be associated with injury risk. PURPOSE: To prospectively examine differences in biomechanical and clinical performance measures in male athletes 9 months after ACLR between those who ruptured their previously uninjured contralateral ACL and those who did not at 2-year follow-up and to examine the ability of these differences to predict contralateral ACL injury. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: A cohort of male athletes returning to level 1 sports after ACLR (N = 1045) underwent isokinetic strength testing and 3-dimensional biomechanical analysis of jump and change-of-direction tests 9 months after surgery. Participants were followed up at 2 years regarding return to play or at second ACL injury. Between-group differences were analyzed in patient-reported outcomes, performance measures, and 3-dimensional biomechanics for the contralateral limb and asymmetry. Logistic regression was applied to determine the ability of identified differences to predict contralateral ACL injury. RESULTS: Of the cohort, 993 had follow-up at 2 years (95%), with 67 experiencing a contralateral ACL injury and 38 an ipsilateral injury. Male athletes who had a contralateral ACL injury had lower quadriceps strength and biomechanical differences on the contralateral limb during double- and single-leg drop jump tests as compared with those who did not experience an injury. Differences were related primarily to deficits in sagittal plane mechanics and plyometric ability on the contralateral side. These variables could explain group membership with fair to good ability (area under the curve, 0.74-0.80). Patient-reported outcomes, limb symmetry of clinical performance measures, and biomechanical measures in change-of-direction tasks did not differentiate those at risk for contralateral injury. CONCLUSION: This study highlights the importance of sagittal plane control during drop jump tasks and the limited utility of limb symmetry in performance and biomechanical measures when assessing future contralateral ACL injury risk in male athletes. Targeting the identified differences in quadriceps strength and plyometric ability during late-stage rehabilitation and testing may reduce ACL injury risk in healthy limbs in male athletes playing level 1 sports. CLINICAL RELEVANCE: This study highlights the importance of assessing the contralateral limb after ACLR and identifies biomechanical differences, particularly in the sagittal plane in drop jump tasks, that may be associated with injury to this limb. These factors could be targeted during assessment and rehabilitation with additional quadriceps strengthening and plyometric exercises after ACLR to potentially reduce the high risk of injury to the previously healthy knee. REGISTRATION: NCT02771548 (ClinicalTrials.gov identifier).

Lead limb loading during a single-step descent in persons with and without a transtibial amputation in the trailing limb.

BACKGROUND: Decreased mechanical work done by the trailing limb when descending a single-step could affect load development and increase injury risk on the leading limb. This study assessed the effect of trailing limb mechanics on the development of lead limb load during a step descent by examining individuals with unilateral transtibial amputations who are known to exhibit reduced work in the prosthetic limb. METHODS: Eight amputees and 10 able-bodied controls walked 5 m along the length of a raised platform, descended a single-step of 14 cm height, and continued walking. The intact limb of amputees led during descent. Kinematic and kinetic data were recorded using integrated motion capture and force platform system. Lead limb loading was assessed through vertical ground reaction force, and knee moments and joint reaction forces. Sagittal-plane joint work was calculated for the ankle, knee, and hip in both limbs. FINDINGS: No differences were found in lead limb loading despite differences in trail limb mechanics evidenced by amputees performing 58% less total work by the trailing (prosthetic) limb to lower the centre of mass (P = 0.004) and 111% less for propulsion (P < 0.001). Amputees descended the step significantly slower (P = 0.003) and performed significantly greater lead limb ankle work (P = 0.017). After accounting for speed differences, initial loading at the knee was significantly higher in the lead limb of amputees versus controls. INTERPRETATION: Increasing lead limb work and reducing forward velocity may be effective compensatory strategies to limit lead limb loading during a step descent, in response to reduced trailing limb work.

The effect of simulated marker misplacement on the interpretation of inter-limb differences during a change of direction task.

The objective assessment of biomechanical asymmetries during movement tasks is used to monitor rehabilitation following anterior cruciate ligament reconstruction (ACLR). Marker placement is an important source of methodological variability within human motion analysis. It is currently unclear how marker placement error effects the interpretation of biomechanical asymmetries throughout post ACLR rehabilitation. The aim of this investigation was to determine the effect of random marker placement variation on the interpretation of inter-limb differences during a change of direction (CoD) task. Forty-seven participants 9 months post-ACLR and fifty uninjured controls completed a 90° CoD task on both limbs. Inter-limb differences in kinematic and kinetic metrics during the CoD stance phase were calculated for both groups using the Vicon Plug-in Gait model, and ACLR subjects were classified as having 'normal' or 'abnormal' inter-limb differences relative to the control group. Simulated random marker displacements based on published marker placement error ranges were then repeatedly applied to the lateral thigh, femoral epicondyle and tibia markers. ACLR inter-limb differences were recalculated each time, allowing the estimation of 95% confidence intervals and minimal identifiable between-session changes. ACLR subjects were also reclassified relative to the control group after each simulation and the percentage of participants to change classification was calculated. Marker displacements caused large deviations in inter-limb difference measures in several variables including hip rotation angle, knee abduction angle and knee abduction moment, thus limiting the ability to identify participants with large inter-limb differences relative to a control group. These findings highlight the challenges in using marker-based biomechanical models to conduct objective assessments of inter-limb differences during CoD tasks.

Whole-Body Change-of-Direction Task Execution Asymmetries After Anterior Cruciate Ligament Reconstruction.

Cutting maneuvers can be executed at a range of angles and speeds, and these whole-body task descriptors are closely associated with lower-limb mechanical loading. Asymmetries in angle and speed when changing direction off the operated and nonoperated limbs after anterior cruciate ligament reconstruction may therefore influence the interpretation of interlimb differences in joint-level biomechanical parameters. The authors hypothesized that athletes would reduce center-of-mass heading angle deflection and body rotation during the change-of-direction stance phase when cutting from the operated limb, and would compensate for this by orienting their center-of-mass trajectory more toward the new intended direction of travel prior to touchdown. A total of 144 male athletes 8 to 10 months after anterior cruciate ligament reconstruction performed a maximum-effort sidestep cutting maneuver while kinematic, kinetic, and ground reaction force data were recorded. Peak ground reaction force and knee joint moments were lower when cutting from the operated limb. Center-of-mass heading angle deflection during stance phase was reduced for cuts performed from the operated limb and was negatively correlated with heading angle at touchdown. Between-limb differences in body orientation and horizontal velocity at touchdown were also observed. These systematic asymmetries in cut execution may require consideration when interpreting joint-level interlimb asymmetries after anterior cruciate ligament reconstruction and are suggestive of the use of anticipatory control to co-optimize task achievement and mechanical loading.

The sensitivity of joint kinematics and kinetics to marker placement during a change of direction task.

The conventional gait model (CGM) refers to several closely related biomechanical models used in the objective analysis of human motion. Their use has become popular in the analysis of change of direction tasks to inform best practice in the prevention and rehabilitation of anterior cruciate ligament injury. As externally-placed markers define segment axes origins and orientations, kinematic and kinetic outputs from the CGM are sensitive to marker placement. The aim of this investigation was to quantify the sensitivity of lower extremity kinematics and knee moments to systematic differences in marker placement across the stance phase of a change of direction task. Systematic anterior/posterior displacements were applied to the lateral thigh, femoral epicondyle and tibia markers in software. One-dimensional statistical parametric mapping was used to determine the effect of marker placement across the entire stance phase of a 90° change of direction task. Marker placement error within previously reported inter-tester variability ranges caused significant differences in knee abduction moment, hip rotation angle, knee rotation angle, ankle abduction and rotation angle across various periods of stance. Discrete measures of these variables have been associated with increased frontal plane knee loading during change of direction, considered a key mechanism of anterior cruciate ligament injury. Systematic differences in marker placement may lead to incorrect group statistical inferences in such discrete measures.

Factors Influencing Return to Play and Second Anterior Cruciate Ligament Injury Rates in Level 1 Athletes After Primary Anterior Cruciate Ligament Reconstruction: 2-Year Follow-up on 1432 Reconstructions at a Single Center.

BACKGROUND: Despite the importance of return-to-play (RTP) rates, second anterior cruciate ligament (ACL) injury rates, and patient-reported outcomes of athletes returning to sports after ACL reconstruction (ACLR), these outcomes have not been evaluated together across a single cohort nor the pre- and intraoperative factors influencing outcomes explored. PURPOSE: To prospectively report outcomes after ACLR relating to RTP, second ACL injury, and International Knee Document Committee (IKDC) scores in a large cohort of athletes at a single center to examine the influence of pre- and intraoperative variables on these outcomes. DESIGN: Cohort study; Level of evidence, 3. METHODS: A consecutive cohort of 1432 athletes undergoing primary ACLR by 2 orthopaedic surgeons was followed up prospectively more than 2 years after surgery. Pre- and intraoperative findings were reported with outcomes at follow-up relating to RTP, second ACL injury, and IKDC. Between-group differences for each outcome were reported and the predictive ability of pre- and intraoperative variables relating to each outcome assessed with logistic regression. RESULTS: There was >95% follow-up 2 years after surgery. The RTP rate was 81%, and of those who returned, 1.3% of those with patellar tendon grafts and 8.3% of those with hamstring grafts experienced ipsilateral rerupture (hazard ratio, 0.17). The contralateral ACL injury rate was 6.6%, and the IKDC score at follow-up was 86.8, with a greater proportion of patients with patellar tendon grafts scoring <80 on the IKDC (odds ratio, 1.56; 95% CI, 1.15-3.12). There was no relationship between time to RTP and second ACL injury, and there was a moderate correlation between ACL-Return to Sport After Injury score and RTP at follow-up (P < .001, rho = 0.46). There were a number of differences in pre- and intraoperative variables between groups for each outcome, but they demonstrated a poor ability to predict outcomes in level 1 athletes at 2-year follow-up. CONCLUSION: Findings demonstrated high overall RTP rates, lower reinjury rates with patellar tendon graft after 2-year follow-up in level 1 athletes, and no influence of time to RTP on second ACL injury. Despite differences between groups, there was poor predictive ability of pre- and intraoperative variables. Results suggest pre- and intraoperative variables for consideration to optimize outcomes in level 1 athletes after ACLR, but future research exploring other factors, such as physical and psychological recovery, may be needed to improve outcome prediction after ACLR. REGISTRATION: NCT02771548 (ClinicalTrials.gov identifier).

Foot strike alters ground reaction force and knee load when stepping down during ongoing walking.

BACKGROUND: When stepping down from a raised surface, either a toe or heel contact strategy is performed. Increased vertical momentum is likely to be experienced during a step descent, yet the extent to which these descent strategies influence the development of load at the ground and knee has not been examined. RESEARCH QUESTION: Does descent strategy influence ground and knee joint loading? Does the contribution from leading and trailing limb joint mechanics differ between descent strategies? METHODS: Twenty-two healthy male participants (age: 34.0 ±â€¯6.5 years, height: 179 ±â€¯6.3 cm, mass: 83.5 ±â€¯13 kg) walked along a raised platform, stepped down from a 14 cm height utilising either a toe (n = 10) or heel (n = 12) initial contact, and continued walking. Vertical ground reaction forces and knee external adduction and flexor moments were extracted for the duration of the braking phase. Joint work was calculated for the ankle, knee, and hip in both the leading and trailing limbs. RESULTS: Waveform analysis of the loading features indicated that a toe-contact strategy resulted in significantly reduced loading rates during early braking (1-32% of the braking phase) and significantly increased magnitude in late braking (55-96% of the braking phase). Individuals performing toe landings completed 33% greater overall work (p = 0.091) in the lead limb and utilised the lead limb ankle joint as the main shock absorber (79% of total lead limb work). Concurrently, the trailing limb performed 29% and 21% less work when lowering the centre of mass and propulsion, respectively, compared to a heel landing. SIGNIFICANCE: A toe-contact strategy results in reduced limb and knee joint loading rates through greater utilisation of the lead limb ankle joint. A heel-contact strategy, however, can reduce loading during late braking by utilising the functionality of the trailing limb.

Mechanisms to Attenuate Load in the Intact Limb of Transtibial Amputees When Performing a Unilateral Drop Landing.

Individuals with unilateral transtibial amputations experience greater work demand and loading on the intact limb compared with the prosthetic limb, placing this limb at a greater risk of knee joint degenerative conditions. It is possible that increased loading on the intact side may occur due to strength deficits and joint absorption mechanics. This study investigated the intact limb mechanics utilized to attenuate load, independent of prosthetic limb contributions and requirements for forward progression, which could provide an indication of deficiencies in the intact limb. Amputee and healthy control participants completed 3 unilateral drop landings from a 30-cm drop height. Joint angles at touchdown; range of motion; coupling angles; peak powers; and negative work of the ankle, knee, and hip were extracted together with isometric quadriceps strength measures. No significant differences were found in the load or movement mechanics (P ≥ .31, g ≤ 0.42), despite deficits in isometric maximum (20%) and explosive (25%) strength (P ≤ .13, g ≥ 0.61) in the intact limb. These results demonstrate that, when the influence from the prosthetic limb and task demand are absent, and despite deficits in strength, the intact limb adopts joint mechanics similar to able-bodied controls to attenuate limb loading.

The effects of long-term muscle disuse on neuromuscular function in unilateral transtibial amputees.

NEW FINDINGS: What is the central question of this study? The effects of long-term muscle disuse on neuromuscular function are unclear because disuse studies are typically short term. In this study, we used a new model (unilateral transtibial amputees) to investigate the effects of long-term disuse on quadriceps neuromuscular function. What is the main finding and its importance? Kinetic analysis (knee-extension moments during gait) indicated habitual disuse of the amputated limb quadriceps, accompanied by lower quadriceps muscle strength (60-76%) and neural activation (32-44%), slower contractile properties and altered muscle architecture in the amputated limb, which could not be predicted from short-term disuse studies. ABSTRACT: The purpose of this study was to determine: (i) whether individuals with unilateral transtibial amputations (ITTAs), who habitually disuse the quadriceps muscles of their amputated limb, provide an effective model for assessing the effects of long-term muscle disuse; and (ii) the effects of such disuse on quadriceps muscle strength and neuromuscular function in this population. Nine ITTAs and nine control subjects performed isometric voluntary knee extensions of both limbs to assess maximal voluntary torque (MVT) and the rate of torque development (RTD). The interpolated twitch technique and EMG normalized to maximal M-wave were used to assess neural activation, involuntary (twitch and octet) contractions to assess intrinsic contractile properties, and ultrasound images of the vastus lateralis to assess muscle architecture. Clinical gait analysis was used to measure knee kinetic data during walking at an habitual speed. The ITTAs displayed 54-60% lower peak knee-extensor moments during walking in the amputated compared with intact/control limbs, but the intact and control limbs were comparable for loading during walking and muscle strength variables, suggesting that the intact limb provides a suitable internal control for comparison with the disused amputated limb. The MVT and RTD were ∼60 and ∼75% lower, respectively, in the amputated than intact/control limbs. The differences in MVT appeared to be associated with ∼40 and ∼43% lower muscle thickness and neural activation, respectively, and the differences in RTD appeared to be associated with the decline in MVT coupled with slowing of the intrinsic contractile properties. These results indicate considerable changes in strength and neuromuscular function with long-term disuse that could not be predicted from short-term disuse studies.

The use of continuous spectral analysis for the assessment of postural stability changes after sports-related concussion.

Impaired postural stability is associated with a variety of pathologies including sports-related concussion (SRC). Quantification of centre of pressure (COP) movement is the most common focus of instrumented assessment. Frequency-domain COP analyses have focused primarily on summary measures or pre-defined frequency bands but continuous analysis may provide novel and complementary insight into pathological control mechanisms. Our aims were (i) to compare post-SRC COP trajectory changes identified using clinician scores (Modified Balance Error Scoring System (M-BESS)), time-domain COP variables and continuous frequency spectral comparison; and (ii) to characterise frequency spectra changes. Male rugby players aged 15-19 years (n = 135) completed a pre-season baseline assessment comprising vision-obscured double-leg, single-leg and tandem stances on a force platform. Participants diagnosed with SRC during the season (n = 15) underwent repeat testing (median 4 days post-SRC; IQR 2.5-6.5). Baseline and post-SRC COP trajectories were compared using common time-domain COP variables, M-BESS scores and continuous frequency spectra. Post-SRC changes were identified using all three approaches. Spectral analysis revealed the largest effect size (Cliff's delta 0.39) and was the only method to identify differences in all three stances and in double-leg stance. All post-SRC increases in spectral content were in the anteroposterior direction; all decreases were in the mediolateral direction. Changes were localised to higher frequencies (1.7-8 Hz) except for double-leg stance anteroposterior direction, for which increases were observed throughout the analysed range. Our findings suggest that this method of spectral comparison may provide a more responsive and meaningful measure of postural stability changes after SRC than other commonly-used variables.

Objective classification and scoring of movement deficiencies in patients with anterior cruciate ligament reconstruction.

Motion analysis systems are widely employed to identify movement deficiencies-e.g. patterns that potentially increase the risk of injury or inhibit performance. However, findings across studies are often conflicting in respect to what a movement deficiency is or the magnitude of association to a specific injury. This study tests the information content within movement data using a data driven framework that was taught to classify movement data into the classes: NORM, ACLOP and ACLNO OP, without the input of expert knowledge. The NORM class was presented by 62 subjects (124 NORM limbs), while 156 subjects with ACL reconstruction represented the ACLOP and ACLNO OP class (156 limbs each class). Movement data from jumping, hopping and change of direction exercises were examined, using a variety of machine learning techniques. A stratified shuffle split cross-validation was used to obtain a measure of expected accuracy for each step within the analysis. Classification accuracies (from best performing classifiers) ranged from 52 to 81%, using up to 5 features. The exercise with the highest classification accuracy was the double leg drop jump (DLDJ; 81%), the highest classification accuracy when considering only the NORM class was observed in the single leg hop (81%), while the DLDJ demonstrated the highest classification accuracy when considering only for the ACLOP and ACLNO OP class (84%). These classification accuracies demonstrate that biomechanical data contains valuable information and that it is possible to differentiate normal from rehabilitating movement patterns. Further, findings highlight that a few features contain most of the information, that it is important to seek to understand what a classification model has learned, that symmetry measures are important, that exercises capture different qualities and that not all subjects within a normative cohort utilise 'true' normative movement patterns (only 27 to 71%).

Back to Normal Symmetry? Biomechanical Variables Remain More Asymmetrical Than Normal During Jump and Change-of-Direction Testing 9 Months After Anterior Cruciate Ligament Reconstruction.

BACKGROUND: After anterior cruciate ligament reconstruction (ACLR), athletes have demonstrated performance asymmetries as compared with healthy cohorts, but little research has investigated if biomechanical asymmetries are also different during jump and change-of-direction (CoD) tasks between groups. PURPOSE: To identify if differences in magnitude of asymmetry of biomechanical and performance variables exist between these groups. STUDY DESIGN: Controlled laboratory study. METHODS: Analysis was conducted between 156 male patients 9 months after surgery and 62 healthy participants. Three-dimensional motion capture and analysis were carried out on a double-legged drop jump, a single-legged drop jump, a single-legged hop for distance, and planned and unplanned CoD. Asymmetry between limbs was calculated for each variable with root mean square difference between limbs. Statistical parametric mapping was used to identify the between-group differences in magnitude of asymmetry of performance and biomechanical variables. RESULTS: There were differences in asymmetry of biomechanical variables across all jump and CoD tests, with greater asymmetries in the ACLR group. The majority of differences between groups were in the sagittal and frontal planes, with more differences found in the jump than CoD tests. The single-legged drop jump demonstrated large differences in performance asymmetry (effect size, 0.94) with small differences for both CoD tests (0.4) and none for the single-legged hop for distance. CONCLUSION: This study demonstrated greater asymmetry of biomechanical variables 9 months after ACLR as compared with healthy participants across all tests, suggesting insufficient rehabilitation. CLINICAL RELEVANCE: This study highlights the importance of including biomechanical as well as performance variables when assessing rehabilitation status after ACLR. REGISTRATION: NCT02771548 ( ClinicalTrials.gov identifier).