[Analysis of the effect of N5, N10-methylenetetrahydrofolate reductase gene C(677)-->T polymorphism on the ischemic stroke development in persons with various risk factors].

The results ofMTHFR gene C(677)-->T (rs1801133) polymorphism determined in 170 patients with ischemic atherothrombotic stroke (IATS) and 124 healthy subjects (control group) are presented in the paper. It has been shown that in patients with IATS, the frequencies of main homozygotes (CC), heterozygotes (CT) and minor homozygotes (TT) are 52.4, 35.9, 11.8% (in control--46.0, 48.4, 5.6%, P = 0.044 by chi2-test). TT homozygotes have a greater chance of developing IATS than carriers of main C-allele (CT + CC) (OR = 2.3, CI = 0.911-5.449, P = 0.049). In the representatives of the Ukrainian population there is a relationship between the frequency of MTHFR gene C(677)-->T polymorphism genotypes and the risk of IATS. This connection is manifested in male patients, in persons with normal blood pressure, and in people who do not have the habit of smoking. The sex of the patients, body mass index, blood pressure and smoking affect the level of the studied polymorphism association with stroke.

[Frequency of allele polymorphism of immune proteasome catalytic subunits in patients with ischemic stroke].

Frequency of allelic variants of proteasome subunits genes LMP2 (Arg60 --> His) and PSMA6 were determined in patients with ischemic stroke using real-time PCR. Allelic variants of PSMA6 were disposed in the next manner: C/C - 80.2%, C/G - 19.8%, G/G--were not (in control) and C/C - 75.5%, C/G - 21.4%, G/G - 3.1% (P = 0.22) in patients with IS. It was shown that distribution of LMP2 allelic variants was the following: Arg/Arg - 53.3%, Arg/His - 43.5%, His/His - 6.7% in control and Arg/Arg - 55.9%, Arg/His - 34.3%, His/His - 9.8% in IS group (P > 0.05). The data show that LMP2 and PSMA6 gene polymorphism is not a risk factor of ischemic stroke in Ukrainian population.