[Phenylpropanoid glycosides from Sophorae Tonkinensis Radix et Rhizoma].

Three phenylpropanoid glycosides were purified by extensive chromatographic techniques including column chromatography over microporous resin, MCI, diol, sephadex LH-20, reverse phase C_(18) from water-extracts of Sophorae Tonkinensis Radix et Rhizoma. Their planar structures were elucidated by combination of various spectroscopic method, such as IR, UV, MS, and NMR. The absolute configuration of aglycone was determined by quantum chemical calculations. Their structures were elucidated as(8R)-3-(4-hydroxyphenyl)-1-propanol-2-O-ß-D-glucopyranoside(1), kalopanaxin D(2),(E)-4-hydroxycinnamyl alcohol 4-O-[2'-O-ß-D-apiofuranosyl(1″→2')]-ß-D-glucopyranoside(3). Compound 1 was undescribed previously. Compounds 2 and 3 were firstly isolated from Sophora genus.

[Study on chemical constituents from Euphorbia thymifolia].

This work was launched to study on the chemical constituents from Euphorbia thymifolia. Thirteen compounds were isolated from the 95% ethanol extract of the aerial parts of E. thymifolia by column chromatographies on silica gel, Sephadex LH-20, MCI, and ODS, and preparative HPLC, including two thymol derivatives(1-2), four alkaloids(3-6), five isocoumarins(7-11), together with two ellagic acids(12-13). All the compounds are listed as follows:(Z)-8,9-dehydro-9,10-diisobutyryloxythymol(1), 8-hydro-xy-9,10-diisobutyryloxythymol(2), N-(N-benzoyl-L-phenylalanyl)-L-phenylalanol(3), aurantiamide acetate(4), 1-carboethoxy-ß-carboline(5), isoechinulin A(6), ethyl brevifolincarboxylate(7), euphorhirtin B(8), 4,5-didehydro chebulic acid triethyl ester(9), euphorhirtin G(10), pomegranatate(11), 3,3',4'-tri-O-methylellagic acid(12), 3,3'-di-O-methylellagic acid(13). Compound 1 is a new compound. Except for compound 4, the others were isolated from this plant for the first time. All the compounds were screened for anti-neuroinflammatory activity in vitro, and compounds 1-3 and 7 showed significant activity with IC_(50) values of 0.19,12.93,7.29,25.4 µmol·L~(-1).

Reliability and validity of the GMFM-66 in 0- to 3-year-old children with cerebral palsy.

OBJECTIVE: To examine the reliability and validity of a 66-item version of the Gross Motor Function Measure (GMFM-66) to assess the gross motor functions of children <3 yrs old with cerebral palsy. DESIGN: 298 valid samples were obtained from 171 children with cerebral palsy (male 126, female 45, mean age 19 mos, age range 3-36 mos) measured with GMFM-88. Then a 73-item version of GMFM (GMFM-73) special for these children was obtained by Rasch analysis. GMFM-66 score and GMFM-73 scores of each sample were obtained. The reliability and validity of GMFM-66 were evaluated by analyzing the correlation between the scores and between the change scores of these two GMFM versions. The relative precision of GMFM-73 vs. GMFM-66 was also analyzed. RESULTS: The test-retest reliability and interscorer reliability of GMFM-66 were both high. The ICC scores were 0.9666 and 0.9782, respectively. Significant correlations were found between the scores (r = 0.9848, P < 0.001) and between the change scores (r = 0.8700, P < 0.001) of these two versions of GMFM. A 14% less gain in relative precision was achieved when using GMFM-73 vs. GMFM-66. CONCLUSION: Results indicated that the GMFM-66 had good reliability and validity in assessing the gross motor functions of children <3 yrs old with cerebral palsy. The GMFM-73 derived in the present study did not function significantly better for young children than GMFM-66.