RESUMO
The intestinal epithelium is the first line of host defense, and its homeostasis is dependent on soluble factors that comprise the crypt niche. Antimicrobial proteins are one of the mediators to maintain gut homeostasis. Angiogenin-4 (Ang4) is a member of the ribonuclease A superfamily and plays a pivotal role in antimicrobial activity against gut microbiota. However, the functions of Ang4 within the intestinal crypt niche, particularly its involvement in the development of intestinal epithelial cells (IECs), remain unknown. Here, we demonstrate that Ang4 plays a significant role in maintaining Lgr5+ intestinal stem cells (ISCs) and induces apoptosis of IECs in a concentration-dependent manner. We revealed that Ang4 is highly expressed by Paneth cells in the small intestine, as well as regenerating islet-derived family member-4 (Reg4) expressing goblet cells in the colon, and both cell subsets highly contribute to ISC maintenance. Functional analysis using intestinal organoids revealed that Ang4 induces Wnt and Notch signaling, increases Lgr5+ stem cell expansion, and promotes organoid growth. Furthermore, high concentrations of Ang4 induced apoptosis in the IEC cell line and organoids. Collectively, we propose that Ang4 is a dual functional protein and is a novel member of the crypt niche factor that promotes the expansion of ISCs and induces apoptosis.
RESUMO
Angiogenin, a well-known angiogenic factor, is crucial to the angiogenesis in gastrointestinal tumors. Human angiogenin has only one gene, whereas the murine angiogenin family has extended to incorporate six genes. Evolutionary studies have suggested functional variations among murine angiogenin paralogs, even though the three-dimensional structures of angiogenin proteins are remarkably similar. In addition to angiogenesis, the ubiquitous pattern of angiogenin expression suggests a variety of functions, such as tumorigenesis, neuroprotective, antimicrobial activity, and innate immunity. Here, we comprehensively reviewed studies on the structures and functions of human and mouse angiogenins. Understanding the structure and function of angiogenins from a broader perspective could facilitate future research related to development of novel therapeutics on its biological processes, especially in gastrointestinal cancers.
RESUMO
The purpose of studying the consequence of COVID-19 on oxbow lake (Baor) fisher's community is to counteract the negative impacts on livelihoods with food security and figure out diversified resilience options for sustaining basic needs of life. Individual questionnaire interviews, oral history, focus group discussion, and telephonic interviews were among the methodological techniques used to gather primary data. The Baor fisher's community was impaired with income, food and feeding habit, health and marketing. The Baor fishers had to stop harvesting or reducing the amount of fish harvest because of gradual decreasing of consumer demand and prices of fish during the course of COVID-19 pandemic period. The transportation costs were raised up to 50%-80%, while the prices of fish decreased by 15%-30% prior to the onset of COVID-19 pandemic. The frequency of fish consumption was significantly come down to 37.5%. Many households substituted fish to farm reared hens, eggs, domestic hens and ducks, lentils, and vegetables during the period of lockdown across the country. Supply chains of fish and fish culture inputswere disrupted due to inadequacy of transportation facilities. Many school- and college-going students were dropped outduring the ongoing pandemic situation due to their financial problems (10%) and early marriage (7.5%). The secondary sources of income (labor of netting in other aquaculture farms) of Baor fisher's community were also impaired. The resilience options of this study will be helpful to minimize the sudden economic crises, ensure dynamic fish value chains and food security, protect individuals from ongoing health hazards, and promote sustainable food production systems followed by social cohesion and stabilityagainst the prevailing challenges owing to the pandemic and other natural calamities.
RESUMO
Angiogenin 4 bearing ribonuclease activity is an endogenous antimicrobial protein expressed in small and large intestine. However, the crucial amino acid residues responsible for the antibacterial activity of Ang4 and its impact on gut microbiota remain unknown. Here, we report the contribution of critical amino acid residues in the functional regions of Ang4 to its activity against Salmonella typhimurium LT2 and the effect of Ang4 on gut microbiota in mice. We found that Ang4 binds S. typhimurium LT2 through two consecutive basic amino acid residues, K58 and K59, in the cell-binding segment and disrupts the bacterial membrane integrity at the N-terminal α-helix containing residues K7 and K30, as evidenced by the specific mutations of cationic residues of Ang4. We also found that the RNase activity of Ang4 was not involved in its bactericidal activity, as shown by the H12 mutant, which lacks RNase activity. In vivo administration of Ang4 through the mouse rectum and subsequent bacterial 16S rRNA gene sequencing analyses demonstrated that administration of Ang4 not only increased beneficial bacteria such as Lactobacillus, Akkermansia, Dubosiella, Coriobacteriaceae UCG-002, and Adlercreutzia, but also decreased certain pathogenic bacteria, including Alistipes and Enterohabdus, indicating that Ang4 regulates the shape of gut microbiota composition. We conclude that Ang4 kills bacteria by disrupting bacterial membrane integrity through critical basic amino acid residues with different functionalities rather than overall electrostatic interactions and potentially maintains gut microflora in vivo under physiological and pathophysiological conditions.
RESUMO
For the first time, we revealed the life-history traits including growth pattern (length-weight relationships, LWRs), condition factors, form factor (a3.0), first sexual maturity (Lm), age at first sexual maturity (tm), life span (tmax), natural mortality (Mw), asymptotic length (L∞), and optimum catchable length (Lopt) of ten commercially important small indigenous fish species (SIFS) in the Oxbow lake (Baor), southwestern regions of Bangladesh. A total of 1651 specimens were sampled during January to December 2020 with traditional fishing gears including seine nets, gill nets, and lift nets. Individual total length (TL) and body weight (BW) were measured by digital slide calipers and digital balance, respectively. To calculate the Lm, empirical maximum length-based model was considered, and Lopt was calculated based on L∞. The TL vs. BW relationship indicated positive allometric growth for Chanda nama (Hamilton 1822), Channa punctata (Bloch 1793), Channa striata (Bloch 1793), Lepidocephalichthys guntea (Hamilton 1822), Macrognathus pancalus (Hamilton 1822), and Puntius sophore (Hamilton 1822), but negative allometric growth for Badis badis (Hamilton 1822), Gudusia chapra (Hamilton 1822), Glossogobius giuris (Hamilton 1822), and Hyporhamphus limbatus (Valenciennes, 1847). All r2 values exceed 0.910 that indicated all LWRs were highly significant (P < 0.001). According to Spearman correlation test, Fulton's condition factor (KF) vs. BW was highly correlated (P < 0.001), indicating better well-being for these species. Moreover, a3.0 indicates B. badis, C. punctata, C. striata, G. giuris, H. limbatus, L. guntea were elongated; C. nama, P. sophore, were short and deep; G. chapra was fusiform, and M. pancalus was eel-like body shape respectively. The minimum tm and tmax were obtained as 0.74 year and 2.66 year for C. striata and maximum were 0.93 year and 3.31 year for B. badis, respectively. This study provided information on tm and tmax for ten SIFS that is globally absent. From empirical models, the smallest mean value of Lm was found for B. badis (3.98 cm), and the greatest was found for C. striata (16.96 cm). The minimum Lopt was obtained as 3.78 cm TL for B. badis and maximum was 14.09 cm TL for C. punctata. The minimum Mw was documented as 1.39 for B. badis and maximum was 1.73 for C. striata. The output of this research will be helpful for developing sustainable management policies and protection of SIFS through the application of mesh size based on Lm and Lopt in the Oxbow lakes, Bangladesh and neighboring countries.