Region Segmentation of Whole-Slide Images for Analyzing Histological Differentiation of Prostate Adenocarcinoma Using Ensemble EfficientNetB2 U-Net with Transfer Learning Mechanism.

Recent advances in computer-aided detection via deep learning (DL) now allow for prostate cancer to be detected automatically and recognized with extremely high accuracy, much like other medical diagnoses and prognoses. However, researchers are still limited by the Gleason scoring system. The histopathological analysis involved in assigning the appropriate score is a rigorous, time-consuming manual process that is constrained by the quality of the material and the pathologist's level of expertise. In this research, we implemented a DL model using transfer learning on a set of histopathological images to segment cancerous and noncancerous areas in whole-slide images (WSIs). In this approach, the proposed Ensemble U-net model was applied for the segmentation of stroma, cancerous, and benign areas. The WSI dataset of prostate cancer was collected from the Kaggle repository, which is publicly available online. A total of 1000 WSIs were used for region segmentation. From this, 8100 patch images were used for training, and 900 for testing. The proposed model demonstrated an average dice coefficient (DC), intersection over union (IoU), and Hausdorff distance of 0.891, 0.811, and 15.9, respectively, on the test set, with corresponding masks of patch images. The manipulation of the proposed segmentation model improves the ability of the pathologist to predict disease outcomes, thus enhancing treatment efficacy by isolating the cancerous regions in WSIs.

Cluster Analysis of Cell Nuclei in H&E-Stained Histological Sections of Prostate Cancer and Classification Based on Traditional and Modern Artificial Intelligence Techniques.

Biomarker identification is very important to differentiate the grade groups in the histopathological sections of prostate cancer (PCa). Assessing the cluster of cell nuclei is essential for pathological investigation. In this study, we present a computer-based method for cluster analyses of cell nuclei and performed traditional (i.e., unsupervised method) and modern (i.e., supervised method) artificial intelligence (AI) techniques for distinguishing the grade groups of PCa. Two datasets on PCa were collected to carry out this research. Histopathology samples were obtained from whole slides stained with hematoxylin and eosin (H&E). In this research, state-of-the-art approaches were proposed for color normalization, cell nuclei segmentation, feature selection, and classification. A traditional minimum spanning tree (MST) algorithm was employed to identify the clusters and better capture the proliferation and community structure of cell nuclei. K-medoids clustering and stacked ensemble machine learning (ML) approaches were used to perform traditional and modern AI-based classification. The binary and multiclass classification was derived to compare the model quality and results between the grades of PCa. Furthermore, a comparative analysis was carried out between traditional and modern AI techniques using different performance metrics (i.e., statistical parameters). Cluster features of the cell nuclei can be useful information for cancer grading. However, further validation of cluster analysis is required to accomplish astounding classification results.