Methods for improving imaging quality of a small-distance high-speed rotational reflection tracking system.

The imaging quality of a rotational reflection high-speed tracking system is greatly affected by the optical characteristics of the reflector and the depth of field limitations of the imaging system, especially for tracking systems working in small distances. In order to improve the imaging quality, this paper focused on two factors that affect the imaging quality: double vision caused by the optical characteristics of reflectors and blurring caused by the depth of field of imaging systems. This paper quantified the impact of these two factors on imaging through theoretical analysis, proposed a method of changing the hardware position, and conducted a simulation and experiments. The results show that the proposed solution in this paper can effectively improve the imaging quality of the system. The content studied in this paper has certain significance in the field of high-speed tracking of rotating reflectors and can provide reference for relevant researchers.

High-speed target tracking control system based on short-time rotational reflection imaging.

High-speed tracking technology has wide applications in the military and aerospace industry. However, existing approaches, such as camera arrays or Doppler radar systems, suffer from high cost and inconvenience. This paper reports a high-speed target tracking control system based on short-time rotational reflection imaging, specifically aimed at overcoming certain limitations. In the system we designed, a high-speed camera coupled with a rotating reflector is used to achieve reliable high-speed target tracking. This paper first introduces the working principle and mathematical model of the system, then analyzes the key technologies, including motor response delay time and rotational speed curve fitting, and, finally, verifies the feasibility of the system and the correctness of the theory based on a series of experiments. Experimental results demonstrated that our work is efficient and accurate in target tracking and image clarity. The developed system demonstrates significant potential for widespread use across military and aerospace sectors. Furthermore, the insights gained from our investigation into key technologies could act as a reference point for fellow researchers in related scientific areas.

The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients.

To investigate the potential of H2-calponin (CNN2) as a serum biomarker for hepatocellular carcinoma (HCC), this study employed the serological analysis of recombinantly expressed cDNA clone (SEREX) technique to identify the presence of CNN2 antibody in the serum of patients with HCC and other tumors. The CNN2 protein was produced through genetic engineering and used as an antigen to determine the positive rate of serum CNN2 autoantibodies via indirect enzyme-linked immunosorbent assay (ELISA). In addition, the mRNA and protein expressions of CNN2 in cells and tissues were evaluated using RT-PCR, in situ RT-PCR, and immunohistochemistry methods. The HCC group exhibited a significantly higher positive rate of anti-CNN2 antibody (54.8%) compared to gastric cancer (6.5%), lung cancer (3.2%), rectal cancer (9.7%), hepatitis (3.2%), liver cirrhosis (3.2%), and normal tissues (3.1%). The positive rates of CNN2 mRNA in HCC with metastasis, non-metastatic HCC, lung cancer, gastric cancer, nasopharyngeal cancer, liver cirrhosis, and hepatitis were 56.67%, 41.67%, 17.5%, 10.0%, 20.0%, 53.13%, and 41.67%, respectively. Meanwhile, the positive rates of CNN2 protein were 63.33%, 37.5%, 17.5%, 27.5%, 45%, 31.25%, and 20.83%, respectively. The down-regulation of CNN2 could inhibit the migration and invasion of liver cancer cells. CNN2 is a newly identified HCC-associated antigen that is implicated in the migration and invasion of liver cancer cells, making it a promising target for liver cancer therapy.

Rapid and flexible calibration of DFPP using a dual-sight fusion target.

The parameter calibration of a digital fringe projection profilometry (DFPP) system is a fundamental step and directly related to 3D measurement accuracy. However, existing solutions based on geometric calibration (GC) suffer from the weakness of limited operability and practicality. In this Letter, a novel, to the best of our knowledge, dual-sight fusion target is designed for flexible calibration. The novelty of this target is the ability to directly characterize control rays for ideal pixels of the projector, and to transform the rays into the camera coordinate system, which replaces the traditional phase-shifting algorithm and avoids the error from the nonlinear response of the system. Attributed to the excellent position resolution of a position-sensitive detector within the target, the geometric relationship between the projector and camera can be easily established by projecting only one diamond pattern. Experimental results demonstrated that the proposed method using only 20 captured images is capable of achieving comparable calibration accuracy to the traditional GC method (20 images versus 1080 images, 0.052 pixels versus 0.047 pixels), which is suitable for rapidly and accurately calibrating the DFPP system in the 3D shape measurement field.

Two-step calibration method of the extrinsic parameters with high accuracy for a bistatic non-orthogonal shafting laser theodolite system.

Motivated by the increasing demands on the precision of 3D large-scale measurement, the extrinsic parameters calibration with high accuracy of the bistatic non-orthogonal shafting laser theodolite (N-theodolite) system is required. A two-step method is proposed to achieve the extrinsic parameters calibration with high accuracy in this paper. In the first step, by analyzing and setting the approximate emitted point during the motion of the laser axis in local space, the calculation of the initial extrinsic parameters can be simplified. In the second step, the above results are taken as the initial values of optimization, and the distances between the spatial laser points provided by PSD sensors with high accuracy in global space are used to construct the unconstrained optimal objective function. The proposed method is validated with the measurement experiment of the bistatic N-theodolite system, the average error of 3D coordinate measurement is less than 0.4 mm, and the average error of distance measurement is less than 0.3 mm within 5 m.

Practical zoom camera calibration method for close-range photogrammetry.

Compared to a conventional fixed focus lens, a zoom lens is more flexible and adaptable. However, the challenges involved in precise calibration for a zoom camera prevent its widespread use in close-range photogrammetry. A practical calibration method for a zoom camera is proposed. The zoom-focus model is established through dimension reduction of the setting variables, which is represented as a set of functions of the zoom setting. The zoom and focus settings are updated in real time for objects at different measurement depths. The calibration process only requires the zoom camera to observe the control points distributed in the designed calibration field with several combinations of zoom and focus settings. All the coefficients of the zoom-focus model can be solved by a nonlinear joint optimization. Experimental results have proved that the proposed method is effective for close-range photogrammetry.

NIR-II Fluorescence Imaging-Guided Oxygen Self-Sufficient Nano-Platform for Precise Enhanced Photodynamic Therapy.

Tumor hypoxia and systemic toxicity seriously affect the efficacy of photodynamic therapy (PDT) and are considered as the "Achilles' heel" of PDT. Herein, to combat such limitations, an intelligent orthogonal emissions LDNP@SiO2 -CaO2 and folic acid-polyethylene glycol-Ce6 nanodrug is rationally designed and fabricated not only for relieving the hypoxic tumor microenvironment (TME) to enhance PDT efficacy, but also for determining the optimal triggering time through second near-infrared (NIR-II) fluorescence imaging. The designed nanodrug continuously releases a large amount of O2 , H2 O2 , and Ca2+ ions when exposed to the acidic TME. Meanwhile, under downshifting NIR-II bioimaging guidance, chlorine e6 (Ce6) consumes oxygen to produce 1 O2 upon excitation of upconversion photon. Moreover, cytotoxic reactive oxygen species (ROS) and calcium overload can induce mitochondria injury and thus enhance the oxidative stress in tumor cells. As a result, the NIR-II bioimaging guided TME-responsive oxygen self-sufficient PDT nanosystem presents enhanced anti-tumor efficacy without obvious systemic toxicity. Thus, the fabricated nanodrug offers great potential for designing an accurate cancer theranostic system.

Zinc application after low temperature stress promoted rice tillers recovery: Aspects of nutrient absorption and plant hormone regulation.

Low temperature during the vegetative stage depresses rice tillering. Zinc (Zn) can promote rice tiller growth and improve plant resistance to abiotic stress. Consequently, Zn application after low temperature might be an effective approach to promote rice tiller recovery. A water culture experiment with treatments of two temperatures (12 °C and 20 °C) and three Zn concentrations (0.08 µM, 0.15 µM and 0.31 µM ZnSO4·7H2O) was conducted to determine by analyzing rice tiller growth, nutrient absorption and hormones metabolism. The results showed that low temperature reduced rice tiller numbers and leaf age, decreased as well. Increasing Zn application after low temperature could enhance not only rice tiller growth rate but also N metabolism and tillering recovery, and correlation analysis showed a significantly positive correlation between tiller increment and Zn and N accumulation after low temperature. In addition, higher cytokinin (CTK)/auxin (IAA) ratio was maintained by promoted synthesis of CTK and IAA as well as enhanced IAA transportation from tiller buds to other parts with increased Zn application after cold stress, which resulted in accelerated germination and growth of tiller buds. These results highlighted that Zn application after low temperature promoted rice tiller recovery by increasing N and Zn accumulation and maintaining hormones balance.

Dynamic calibration and compensation method of a large-scale laser beam based on specular reflection for a nonorthogonal shaft laser theodolite measurement system.

The nonorthogonal shaft laser theodolite (N-theodolite) measurement system is a new kind of instrument that can be applied to large-scale metrology. Different from traditional theodolites, the three axes of the N-theodolite have no requirements on the orthogonality and intersection. For the measurement in laser scale, the parameters of the system on a large scale must be known. A calibration method of the laser beam based on specular reflection and its compensation are proposed, which are at a range of 12 m. Through the experiments, the results show that the straightness accuracy of the laser beam of the left N-theodolite is 0.12 mm at a range of 12 m, and the right one is 0.14 mm. Through the measurement experiments, the maximum measurement deviation of the system is 0.27 mm, which is verified to be a feasible method to calibrate the laser beam in large-scale space.

Calibration method of a laser beam based on discrete point interpolation for 3D precision measurement.

A laser beam used as a visualizing measuring axis is an important technique in 3D shape measurement. A highly accurate calibration method of a laser beam based on discrete point interpolation is proposed in this paper. A flexible control field constructed by a laser tracker, a theodolite and a target plane with 5 high-precision machining holes is presented. The discrete point interpolation model is established by the coordinates of holes measured by a laser tracker and the angles of holes measured by a theodolite. The coordinates of laser spots on the target plane are obtained based on the angles and discrete point interpolation model, and the direction vector of the laser beam is obtained by linear fitting. The optimal measurement pose of a theodolite is analyzed by the simulation results. The experimental results show that the RMSE of linear fitting of laser beams is no more than 14 µm within a 5 m distance, the RMSE of the spatial points is 0.09 mm and the RMSE of the reconstructed distance is 0.09 mm.