RESUMO
Background: In this paper, the method of designing a noninvasive device for eliminating hand tremors in Parkinson's patients is presented. The designed device measures the tremors of the patient's hand and implements the tremor control accordingly. Since Parkinson's disease reduces patients' abilities to perform daily activities, this device is designed as an electronic spoon. The inertial measurement units are used to measure hand tremors. Method: The signals got from motion sensors are passed through Butterworth's second order low pass filters to attenuate signals amplitude at frequencies higher than the human hand's natural frequency. The signals are sent to a proposed Proportional Integral (PI) fuzzy controller as a set point signal, and appropriate control signals are applied to two actuators installed orthogonal. Besides motion sensors, a microcontroller is installed inside the spoon handle that implements a PI fuzzy controller and provides control signals for two high speed servo motors installed perpendicularly. Results: As such, the spoon can minimize the tremor effect. In this system, no damper or mass is added to the hand, and the patients are not required to wear an orthosis. The contribution of this paper is twofold. First, we use sensor data fusion to increase measurement accuracy. In this paper, we use accelerometer and gyroscope sensors. Second, we proposed a robust PI fuzzy controller to compensate for the uncertainties and reduce the tremor. Conclusion: The test results show that the hand tremor of Parkinson's patients during eating is reduced up to 75% using this method.
RESUMO
Toxoplasmosis is a serious parasitic infection and novel therapeutic options are highly demanded to effectively eliminate it. In current study, Toxoplasma gondii myosin A, C and F genes were knocked down using small interference RNA (siRNA) method and the parasite survival and virulence was evaluated in vitro and in vivo. The parasites were transfected with specific siRNA, virtually designed for myosin mRNAs, and co-cultured with human foreskin fibroblasts. The transfection rate and the viability of the transfected parasites were measured using flow cytometry and methyl thiazole tetrazolium (MTT) assays, respectively. Finally, the survival of BALB/c mice infected with siRNAs-transfected T. gondii was assessed. It was demonstrated that a transfection rate of 75.4% existed for siRNAs, resulting in 70% (P = 0.032), 80.6% (P = 0.017) and 85.5% (P = 0.013) gene suppression for myosin A, C and F in affected parasites, respectively, which was subsequently confirmed by Western blot analysis. Moreover, lower parasite viability was observed in those with knocked down myosin C with 80% (P = 0.0001), followed by 86.15% (P = 0.004) for myosin F and 92.3% (P = 0.083) for myosin A. Considerably higher mouse survival (about 40 h) was, also, demonstrated in mice challenged with myosin siRNA-transfected T. gondii, in comparison with control group challenged with wild-type parasites. In conclusion, myosin proteins knock down proposes a promising therapeutic strategy to combat toxoplasmosis.