Identification and Quantitation of Hydroxy Fatty Acids in Fermented Sausage Samples.

The quality of fermented sausage is strongly influenced by its fatty acid (FA). However, the role of a defined starter culture in modifying sausage FA composition, and especially in the production of hydroxy FAs (HFAs), has not been determined. In this study, the FA compositions of sausages fermented with Latilactobacillus sakei, with L. sakei plus Staphylococcus carnosus, and with an aseptic control were characterized by liquid chromatography-mass spectrometry (MS)/MS and gas chromatography-MS. The sausages fermented with L. sakei, and with L. sakei plus S. carnosus, showed a reduced accumulation of poly and/or diunsaturated FAs and distinct composition of HFAs compared to the aseptic control. 2-HFAs were enriched via high-speed counter-current chromatography and identified uniquely in the L. sakei plus S. carnosus fermented sausage. Through lipid analyses, this study illustrated how the choice of a defined starter culture affected the observed FA metabolism in fermented sausages, facilitating the development of starter cultures or additives that impart desirable characteristics to sausage.

Effect of Mixed Cultures of Yeast and Lactobacilli on the Quality of Wheat Sourdough Bread.

In this study, mixed starter cultures of yeast and lactobacilli were used for type I sourdough bread making to evaluate their ability to improve bread quality and increase the amount of flavor volatiles. Kazachstania humilis, Saccharomyces cerevisiae, Wickerhamomyces anomalus, and Lactobacillus sanfranciscensis DSM20451T and Lactobacillus sakei LS8 were used in different combinations to ferment wheat sourdough. S. cerevisiae produced the highest amount of CO2 among all strains and thus enhanced bread volume and crumb texture. S. cerevisiae also increased the free thiol level in bread dough, and this study confirms that thiol accumulation was not strongly related to the content of the glutenin macropolymer (GMP) or bread volume. The role of thiol exchange reactions on bread quality differs between long fermentation sourdough and straight dough with baker's yeast only. The influence of different starter cultures on wheat sourdough bread volatiles was established by using head space solid-phase microextraction and gas chromatography/mass spectrometry analysis (SPME-GC/MS). The sourdough breads fermented with a combination of lactobacilli and yeast had a more complex profile of volatiles, particularly with respect to esters.

Effect of Glutathione Dehydrogenase of Lactobacillus sanfranciscensis on Gluten Properties and Bread Volume in Type I Wheat Sourdough Bread.

Disulfide exchange reactions in wheat dough impact the polymerization of gluten and the volume of bread. In wheat sourdoughs fermented with Lactobacillus sanfranciscensis, glutathione reductase activity accumulates thiols. This study investigated the role of glutathione reductase and NADH oxidase of L. sanfranciscensis on disulfide exchange reactions, peroxide levels, and gluten polymerization in type I wheat sourdoughs. Fermentation with L. sanfranciscensis DSM20451 Δ gshR lacking glutathione reductase activity reduced the thiol levels in dough when compared to the wild type strain L. sanfranciscensis DSM20451. Fermentation with any strain reduced peroxides in wheat doughs when compared to a chemically acidified control. The impact of baker's yeast on thiol and peroxide levels was greater than the impact of lactobacilli. The depolymerization of gluten proteins was dependent on the free thiol content and dough acidification. Bread produced with the glutathione reductase deficient mutant strain had the highest specific volume compared with all the other groups.