Clinical Study on the Efficacy of Laparoscopic Hepatectomy and Its Influence on the Expression of Serum VEGF, FGF, and Immune Function.

Liver cancer is one of the most common malignant tumors. Partial hepatectomy is the most basic and effective treatment for hepatocellular carcinoma because of its high operative effect and perioperative safety. Open surgery is the most traditional hepatectomy. Although it can completely remove tumor lesions and prolong patient survival, it has disadvantages such as large trauma and long postoperative recovery time. Meanwhile, long-term bed rest can increase the risk of complications such as venous thrombosis and infection. The advantages of laparoscopic partial hepatectomy, such as clear operative field, simple operation, little trauma, light surgical stress, quick postoperative recovery, and low complications, can avoid damage to vital organs, blood vessels, and nerves, which has been widely accepted and recognized in clinical practice.

[Inhibitory effects of transcription factor E2F-1 siRNA on invasion and proliferation of gastric cancer cell line MGC803].

BACKGROUND & OBJECTIVE: As an important regulatory factor of cell cycle, transcription factor E2F-1 is closely related to tumorigenesis. This study was to investigate the effects of E2F-1 small interfering RNA (siRNA) on invasion and proliferation of human gastric cancer MGC803 cells. METHODS: E2F-1 siRNA vector containing short hairpin structure was transfected into MGC803 cells. Untransfected and pSilencer4.1-negative-transfected cells were used as controls. The expression of E2F-1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Cell matrigel invasion assay and cloning assay were used to detect the invasion and proliferation of MGC803 cells after E2F-1 siRNA transfection. RESULTS: At 48 h after E2F-1 siRNA transfection, the mRNA level of E2F-1 was down-regulated by over 90.0% of controls; the protein level of E2F-1 was down-regulated by 79.6% of negative control and by 81.5% of empty control. The number of migrated cells was significantly smaller in E2F-1 siRNA group than in negative and empty control groups (18.0+/-2.6 vs. 48.0+/-4.6 and 54.0+/-5.6, P<0.05). The number of cell clones was also significantly smaller in E2F-1 siRNA group than in negative and empty control groups (46.0+/-2.0 vs. 122.3+/-1.5 and 128.7+/-2.1, P<0.05) CONCLUSION: E2F-1 siRNA could down-regulate E2F-1 expression in human gastric cancer MGC803 cells and suppress its invasion and proliferation to some extent.