Selective fluorescence detection of small-molecule-binding proteins by using a dual photoaffinity labeling system.

PHOTO OPPORTUNITY: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.

Active/inactive dual-probe system for selective photoaffinity labeling of small molecule-binding proteins.

Two are better than one: A new approach to selective photoaffinity labeling is described in which a bioactive probe is used in combination with its inactive analog as a scavenger of nonspecific proteins.