The heterogeneity of regional specific ventilation is unchanged following heavy exercise in athletes.

Heavy exercise increases ventilation-perfusion mismatch and decreases pulmonary gas exchange efficiency. Previous work using magnetic resonance imaging (MRI) arterial spin labeling in athletes has shown that, after 45 min of heavy exercise, the spatial heterogeneity of pulmonary blood flow was increased in recovery. We hypothesized that the heterogeneity of regional specific ventilation (SV, the local tidal volume over functional residual capacity ratio) would also be increased following sustained exercise, consistent with the previously documented changes in blood flow heterogeneity. Trained subjects (n = 6, maximal O2 consumption = 61 ± 7 ml·kg(-1)·min(-1)) cycled 45 min at their individually determined ventilatory threshold. Oxygen-enhanced MRI was used to quantify SV in a sagittal slice of the right lung in supine posture pre- (preexercise) and 15- and 60-min postexercise. Arterial spin labeling was used to measure pulmonary blood flow in the same slice bracketing the SV measures. Heterogeneity of SV and blood flow were quantified by relative dispersion (RD = SD/mean). The alveolar-arterial oxygen difference was increased during exercise, 23.3 ± 5.3 Torr, compared with rest, 6.3 ± 3.7 Torr, indicating a gas exchange impairment during exercise. No significant change in RD of SV was seen after exercise: preexercise 0.78 ± 0.15, 15 min postexercise 0.81 ± 0.13, 60 min postexercise 0.78 ± 0.08 (P = 0.5). The RD of blood flow increased significantly postexercise: preexercise 1.00 ± 0.12, 15 min postexercise 1.15 ± 0.10, 45 min postexercise 1.10 ± 0.10, 60 min postexercise 1.19 ± 0.11, 90 min postexercise 1.11 ± 0.12 (P < 0.005). The lack of a significant change in RD of SV postexercise, despite an increase in the RD of blood flow, suggests that airways may be less susceptible to the effects of exercise than blood vessels.

Premenopausal women exhibit an inherent protection of endothelial function following a high-fat meal.

Endogenous estrogens likely increase blood flow and subsequently shear stress but have also been associated with improved endothelial function and cardiovascular protection. In contrast, a high-fat meal is thought to reduce endothelial function and increase cardiovascular risk. Therefore, we tested the hypotheses that fluctuating hormones across the menstrual cycle (1) facilitate an increase in shear rate and explain phase-specific differences in flow-mediated dilation (FMD) and (2) provide vascular protection against the insult of a high-fat meal. Flow-mediated dilation was determined at baseline and 4 hours following a high-fat meal in young women during the menses (M), follicular (F), and luteal (L) phases of the menstrual cycle. Male control participants were studied once. 17ß-Estradiol was elevated (P < .05) during the F (5.3 ± 0.7 pg/mL) and L (5.2 ± 0.6 pg/mL) phases when compared to the M (3.9 ± 0.5 pg/mL) phase, and this was accompanied by an elevated FMD in the F and L phases (12.4 ± 1.4% and 11.2 ± 0.9%, respectively) compared to M (8.0 ± 0.9) with no change in shear rate. Female postprandial FMD was similar throughout the menstrual cycle, while men exhibited a 50% reduction (6.4 ± 1 to 3.3 ± 1%; P < .05). Interestingly, the postprandial FMD response was not associated with concentrations of either 17ß-estradiol or progesterone. Despite acutely changing ovarian hormones across the menstrual cycle, shear stress is invariant and therefore does not account for the changes in FMD. Additionally, young women appear to have an inherent vascular protection from the insult of a high-fat meal, perhaps helping to explain sex-specific differences in cardiovascular risk.

The effect of oral antioxidants on brachial artery flow-mediated dilation following 5 and 10 min of ischemia.

In light of the current methodological developments in flow-mediated dilation (FMD) testing and the recognition that oxidative stress may play an important role in regulating this process, the present study sought to: (1) compare flow-mediated dilation (FMD) following 5 and 10 min of forearm cuff occlusion, and (2) evaluate the role of oxidative stress on vasodilation, both distal and proximal to the cuff. Of the 14 subjects studied, 6 partook solely in a validation study of the antioxidant cocktail (AOC; vitamins C, E, and alpha-lipoic acid), while the remaining 8 subjects underwent FMD assessment in response to 5 and 10 min of forearm occlusion following ingestion of AOC or placebo. Although the efficacy of the AOC was clearly documented by elevated plasma ascorbate levels (approximately 95%) and a reduced free radical concentration (approximately 65%), no effects of acute oral antioxidants were observed. FMD was significantly augmented in response to 10 min of forearm occlusion when compared to 5 min, whether expressed as % change (10.1 +/- 2 vs. 4.5 +/- 1%, respectively) or absolute change in diameter (0.035 +/- 0.005 vs. 0.018 +/- 0.005 cm, respectively). Additionally, post-occlusion shear rate (28,640 +/- 2,799 vs. 18,629 +/- 1,724/s, AUC), FMD/shear rate (approximately 50%), and time to peak dilation (68 +/- 7 vs. 53 +/- 8 s) were greater following 10 min of occlusion. In contrast to previous studies, this investigation has identified a greater brachial artery FMD in response to 10 versus 5 min of forearm ischemia, which appears to be unexplained by oxidative stress.

Comparison of mechanomyographic amplitude and mean power frequency for the rectus femoris muscle: cycle versus knee-extensor ergometry.

The purpose of this study was to compare the mechanomyographic (MMG) amplitude and mean power frequency (MPF) versus power output relationships for the rectus femoris (RF) muscle during incremental cycle (CE) and knee-extensor (KE) ergometry in the same subjects. Eight men performed incremental CE and KE tests to exhaustion while the MMG signal was recorded from the RF muscle. Polynomial regression analyses on the composite and subject-by-subject basis indicated that the relationship between MMG amplitude versus power output was best-fit with a linear model for the composite data and each subject for the KE test, whereas the relationship was best-fit with a linear model for the composite data, but either linear, quadratic, or cubic for the CE test. In addition, there were no consistent patterns of responses for either the CE or KE test for MMG MPF. These results suggest that KE rather than traditional CE exercise may be an optimal mode of examining MMG amplitude for the RF muscle.

Comparison of electromyographic responses for the superficial quadriceps muscles: cycle versus knee-extensor ergometry.

The purpose of this study was to compare the electromyographic (EMG) amplitude and mean power frequency (MPF) versus power output relationships for the three superficial quadriceps muscles during incremental cycle (CE) and knee-extensor (KE) ergometry in the same subjects. Eight men performed incremental CE and KE tests to exhaustion. Surface EMG signals were recorded simultaneously from the vastus lateralis (VL), rectus femoris (RF), and vastus medialis (VM). Polynomial regression analyses on a subject-by-subject basis indicated that the relationship between EMG amplitude versus power output was best-fit with either a linear, quadratic, or cubic model for CE, whereas the relationship was best-fit with a linear model for all subjects for the KE test. No consistent relationship was found for EMG MPF within subjects and between muscle groups for CE or KE. Compared with CE, however, the EMG amplitude during KE exercise was, on average, approximately 87% and 30% higher for the RF and VM muscles, respectively. These results suggest that KE exercise may be a better mode of examining EMG amplitude in the quadriceps muscle during incremental exercise than traditional CE.