RESUMO
Proteopedia (proteopedia.org) is an open resource to explore the structure-function relationship of proteins and other biomolecules. This guide provides practical advice on how to incorporate Proteopedia into teaching the structure and function of proteins and other biomolecules. For 11 activities, we discuss desired outcomes, setting expectations, preparing students for the tasks, using resources within Proteopedia, and evaluating student work. We point out features of Proteopedia that make it especially suitable for teaching and give examples of how to avoid common pitfalls.
Assuntos
Proteínas , Estudantes , Humanos , EnsinoRESUMO
A novel supramolecular host for the uranyl carbonate complex has been designed and synthesized. The modified cyclodextrin host binds uranyl carbonate in water with a stability of 253 M(-1).
Assuntos
Carbonatos/química , Ciclodextrinas/química , Urânio/química , Carbonatos/análise , Etilenodiaminas/química , Água Doce/química , Ligantes , Espectroscopia de Ressonância Magnética , Ligação Proteica , Prótons , Água do Mar/química , TermodinâmicaRESUMO
The amonabactins are a series of four bis(catecholate) siderophores isolated from the pathogenic organism, Aeromonas hydrophila. As tetradentate ligands, they cannot singly satisfy the octahedral coordination sphere of iron. The solution coordination chemistry of the amonabactins has been elucidated using potentiometric and spectrophotometric titrations, circular dichroism, and mass spectroscopy. They form 2:3 metal:ligand complexes at high pH and excess ligand. Their complexation behavior is essentially identical to one another, with log beta(230) = 86.3. At lower pH, they preferentially form a 1:1 bis(catecholato)bis(aqua) iron(III) species, with log beta(110) = 34.3. The 2:3 complexes show a very slight Delta preference in chirality at the metal center, while the 1:1 complexes are achiral. The biological implications of these properties are discussed.