Gel dressing based on type I collagen modified with oligourethane and silica for skin wound healing.

Cutaneous wound healing is a complex process that leads the skin reparation with the formation of scar tissue that typically lacks skin appendages. This fact drives us to find new strategies to improve regenerative healing of the skin. This study outlines, the contribution of colloidal silica particles and oligourethane crosslinking on the collagen material properties and the effect on skin wound healing in rats. We characterized the gel properties that are key forin-situgelation, which is accomplished by the latent reactivity of oligourethane bearing blocked isocyanate groups to crosslink collagen while entrapping silica particles. The swelling/degradation behavior and the elastic modulus of the composite gel were consistent with the modification of collagen type I with oligourethane and silica. On the other hand, these gels were characterized as scaffold for murine macrophages and human stem cells. The application of a composite gel dressing on cutaneous wounds showed a histological appearance of the recovered skin as intact skin; featured by the epidermis, hair follicles, sebaceous glands, subcutaneous adipose layer, and dermis. The results suggest that the collagen-based composite dressings are promising modulators in skin wound healing to achieve a regenerative skin closure with satisfactory functional and aesthetic scars.

Molar superior con dos raíces palatinas: descripción de un caso / Upper molar with two palatine roots: a case description / Molar superior com duas raízes palatinas: uma descrição do caso

Antecedentes: La morfología radicular en molares superiores presenta menos variabilidad anatómica, sin embargo, es muy importante tener en cuenta que existen alteraciones anatómicas, y que aun no están bien fundamentadas, se asocia a hiperactividad en la vaina epitelial de Hertwig o bien asociados a factores externos durante la odontogénesis. Descripción del caso: El presente artículo relata un caso de un segundo molar superior derecho con pulpitis irreversible sintomática, en el examen radiográfico se observa la existencia de una raíz supernumeraria palatina. Se realizó tratamiento endodontico con ProtaperNext® y se obturó con técnica lateral modificada con ultrasonido. Conclusión: Los molares con raíces supernumerarias requieren del conocimiento y experiencia del clínico para integrar los métodos de diagnóstico tales como exámenes radiográficos, tomográficos, de microscopía así como técnicas para el tratamiento endodóntico.

Background: Radiographic examination in upper molars is often deficient in order to diagnose abnormalities in the root canal system and to be able to choose the ideal treatment. Description of the case: This article shows the clinical case of a second right upper molar with symptomatic irreversible pulpitis. An accessory root on the palatal is observed in a radiographic examination. A therapy of the canal was carried out with ProtaperNext ® and the sealing was made by cold lateral condensation with ultrasonic condensation. Conclusión: It is very important to know the root canal system morphology of the tooth to be treated in order to offer the patient an adequate treatment.

Antecedentes: O exame radiográfico em molares superiores é frequentemente deficiente para diagnosticar anormalidades no sistema de canais radiculares e para poder escolher o tratamento ideal. Descrição do caso: Este artigo mostra o caso clínico de um segundo molar superior direito com pulpite irreversível sintomática. Uma raiz acessória no palato é observada em um exame radiográfico. A terapia do canal foi realizada com ProtaperNext ® e a vedação foi feita por condensação lateral fria com condensação ultra-sônica. Conclusão: É muito importante conhecer a morfologia do sistema de canais radiculares do dente a ser tratado, a fim de oferecer ao paciente um tratamento adequado.

Dens invaginatus: reporte de un caso clínico / Dens invaginatus: case report

RESUMEN Introducción: De las malformaciones dentales una de las más frecuentes es el dens in vaginatus o dens in dente, esta alteración anatómica representa un reto para el clínico, debido a su anatomía compleja; sin embargo, gracias a los auxiliares de diagnóstico como la tomografía cone-beam, podremos planear el tratamiento para ofrecer el mejor pronóstico. El presente artículo relata un caso clínico de dens in dente presente en diente veintiuno, con necrosis pulpar y periodontitis apical asintomática. Se solicitó TAC cone-beam, se diagnosticó como tipo II según Oehlers; es instrumentado, irrigado con ultrasonido y obturado con técnica «onda continua de calor¼, con un éxito radiográfi co a seis meses de evolución. Actualmente, gracias a los métodos de diagnóstico y auxiliares de tratamiento tales como el ultrasonido, los sistemas de inyección de gutapercha, se pueden ofrecer tratamientos más efi cientes en la terapéutica endodóncica.

ABSTRACT Introduction: The Dens Invaginatus or Dens in dent it is the most common dental abnormal structure, this anatomic variation means a true clinic challenge because of its complex anatomy, however , thanks to the auxiliary tools for diagnosis just like Cone Beam Computerized Tomography, we can plan a good treatment to offer the best diagnosis. This article describes a clinic case about Dens in Dent in twenty one tooth with pulp necrosis and asymptomatic apical periodontitis. Cone Beam Computerized Tomography was requested, the image helped to classify the kind of Dens in Dent which is type II according to Oehlers´s classifi cation. It is instrumented, irrigated with ultrasound and sealed with "continuous wave of heat "technique. This technique ensures a radiographic success with six months of evolution. Currently, thanks to diagnostic methods and treatment aids such as ultrasound, gutta-percha injection system more effi cient treatments can be offered in endodontic therapeutics.

Immunohistochemical patterns in different stromal variants of pleomorphic adenomas: literature review.

Pleomorphic adenoma is the most frequent type of benign salivary neoplasm located in the mouth and is characterized by its significant histopathological diversity. The histogenesis of the pleomorphic adenoma is uncertain; so far several studies suggest that myoepithelial cells are responsible for the variable histomorphology of this type of neoplasm. At times, stroma is the predominant element. The purpose of the present review is to analyze the results reported in the scientific literature concerning immunomarkers expressed in the different stromal elements of pleomorphic adenoma.

Análisis de Patrones de Cierre Velofaríngeo en Pacientes con Labio y Paladar Hendido / Pattern Analysis of Velopharyngeal Closure in Patients With Cleft Lip and Palate

El objetivo fue identificar los patrones de Cierre Velofaríngeo (CVF) en pacientes con Paladar Hendido (PH) y Labio y Paladar Hendido (LPH) que acuden a la Clínica de Atención Integral de Pacientes con Labio y Paladar Hendido (CAIPLPH) de la Escuela Nacional de Estudios Superiores, Unidad León (ENES, León). Estudio transversal, con una muestra por conveniencia de 79 pacientes entre 4 y 10 años de edad. Las variables incluidas fueron el patrón de CVF, tipo de hendidura, edad del paciente y el antecedente de intervención quirúrgica previa para el cierre de la hendidura palatina. A los pacientes se les realizó valoración clínica y nasofibroscopía por examinadores previamente estandarizados para conocer si existe una relación entre edad y patrón de CVF, entre el tipo de hendidura y patrón de CVF y una relación entre el patrón de CVF y el antecedente quirúrgico, utilizando una prueba bivariada de Chi cuadrada. Se examinaron 46 hombres y 33 mujeres con una media de edad 6,6 años. La frecuencia de los patrones de CVF fueron coronal 8,86%, sagital 67,08%, circular 13,92% y circular con rodete de Passavant 10,12%. Se observó una diferencia estadística significativa entre el patrón de CVF y el tipo de hendidura (X2= 53,93, p<0,001). Las diferentes formas en que se pueden presentar las hendiduras de paladar y labiopalatinas influyen en la función del CVF ya que se puede presentar un patrón distinto en cada caso. El patrón de CVF no se ve afectado por la edad ni por el antecedente quirúrgico de cierre de la hendidura palatina.

The objective of the study was to identify patterns of velopharyngeal closure (CVF) in patients with Cleft Palate (PH) and cleft lip and palate (LPH) attending the Clinic of Comprehensive Attention of Patients with Cleft Lip and Palate (CAIPLPH) of the Escuela Nacional de Estudios Superiores, Unidad León (ENES, León). A cross-sectional study and convenience sample of 79 patients was carried out, including those aged between 4 and 10 years. The variables included were: CVF pattern, kind of cleft, the patient's age and history of previous surgery for closure of the palatal cleft. Patients underwent clinical evaluation and nasofibroscopy by examiners previously standardized to know if there is a relationship between age and CVF pattern, between kind of cleft and pattern of CVF and a relationship between the pattern of CVF and patient's history of previous surgery, using a X2 test bivariate square. Forty-six men and 33 women with a mean age 6.6 years were examined. Frequency patterns CVF: 8.86% coronal, sagittal 67.08%, circular 13.92% and a circular with Passavant ridge 10.12%. A statistically significant difference between the pattern of CVF and the kind of cleft (X2= 53.93, p <0.001) was observed. The different ways in which the PH and LPH clefts can present influence the function of CVF as it can present a different pattern in each case. CVF pattern is unaffected by age or by the previous surgical closure.

Sialolipoma of minor salivary glands: presentation of five cases and review of the literature with an epidemiological analyze.

UNLABELLED: Sialolipoma is rare benign neoplasm arise from salivary glands (majors and minors) characterized by neoplastic adipose tissue with scattered non-neoplastic salivary gland acinus. To date 60 cases (including 5 cases reported in the present paper) have been reported in scientific literature. This article presents 5 new cases of sialolipoma affecting minor salivary glands (MiSG) and additionally reviews and analyzes the previously published cases to assess possible demographical differences between sialolipoma from minor and from major salivary glands. CASE REPORTS: 5 cases (3 females; 2 males; age means 63.8 years), of sialolipoma from MiSG, are reported. 2 of them were located in buccal mucosa, 1 in upper lip mucosa, 1 in floor of the mouth and 1 in retromolar area. All tumors were composed by neoplastic adipocytes cells interlaced with normal salivary gland acinus cover it by a fibrous tissue capsule. Analyzes of literature showed that MiSG sialolipoma is most frequent in females over 60 years old, therefore and in conclusion this article assess different demographical profile of sialolipoma in respect to their topography.

Dientes supernumerarios en dentición primaria asociados a pólipos palatinos: Reporte de caso / Supernumerary teeth in primary dentition associated to palatal polyps: Case report

Los pólipos son poco frecuentes en niños. En este artículo se presenta un caso clínico de un niño de un año dos meses que acude a la Clínica de Odontopediatría de la DEPeI UNAM con dos pólipos fibroepiteliales palatinos ubicados a ambos lados de la papila incisiva, 10 días posteriores a la excisión quirúrgica se observó la erupción de un diente supernumerario en el paladar, y 25 días después se observó la erupción de un segundo diente supernumerario. Ambos dientes fueron extraídos. El diagnóstico histológico de las lesiones en el paladar fue: fibroma de fibroblastos gigantes; sin embargo, no se encontró evidencia histológica que mostrara alguna posible relación entre la presencia de los pólipos palatinos y los dientes supernumerarios.

Polyps are rare in children. The present article reports the clinical case of a 14 month old male patient brought for treatment to the Pedodontics Clinic of the Graduate School, National School of Dentistry National University of Mexico. He presented two palatal fibro-epithelial polyps, located at both sides of the incisive papilla. 10 days after surgical excision, a supernumerary tooth erupted in the palate. 25 days later, eruption of a second supernumerary tooth was observed. Both teeth were extracted. Histological diagnosis of palatal lesions was giant fibroblast fibroma. Nevertheless, no histological evidence was found to show possible relationship between presence of palatal polyps and supernumerary teeth.

Tratamiento de conductos de un densinvaginatus: reporte de un caso / Root Canal Therapy of a Dens Invaginatus: A Case Report

El diente invaginado es una malformación que resulta probablemente de un desdoblamientode la papila dental durante el desarrollo dentario. Los dens invaginatus son dientes clínicamentesignificativos, debido a la gran facilidad de afección de la pulpa. La profundidaddel defecto estructural varía de una leve ampliación del cíngulo a una invaginación quese extiende al ápice. El presente artículo relata el caso de una mujer de veintitrés años deedad, quien refiere una fístula de diez años de evolución en la mucosa en el fondo de sacoa la altura del diente lateral superior izquierdo (diente 22). Luego de la exploración clínica yradiográfica se diagnostica un dens in dente unilateral con necrosis pulpar y absceso apicalcrónico, para lo cual se realizó terapia de conductos y cirugía apical con éxito radiográficode dos años de evolución...

Invaginated tooth is a malformation likely resulting from a splitting of dental papilla duringtooth development. Dens invaginatus are clinically important teeth due to the easy affectionof the dental pulp. Invagination depth varies from a slight enlargement of the cingulum untoan extended invagination to the apex. This article reports the case of a female patient aged23, who referred fistula with ten years of evolution in tooth 22. The clinical and radiographicdiagnosis was dens in dent with necrosis and chronic suppurative periapical periodontitis.The root canal therapy and apical surgery were performed showing successfully radiographicevolution within a two-year follow-up period...

Vascular leiomyoma of the oral cavity. Clinical, histopathological and immunohistochemical characteristics. Presentation of five cases and review of the literature.

Leiomyoma, a benign neoplasia arising from smooth muscle is an uncommon neoplasia of the oral cavity. The most common histological subtype in the oral cavity is the vascular one. To supplement information on vascular leiomyoma of the oral cavity (VLOC), we present cases of VLOC describing their clinical, histological, and immunohistochemical characteristics. Case reports. Five cases of VLOC (3 females; 2 males) from the Clinical and Experimental Pathology Laboratory, Dental School, National Autonomous University of México, are included. The most frequent clinical characteristic of VLOC was a single, asymptomatic, slow growing nodule. The age average of the cases was 40.6, however 3 out of our 5 cases were < or = 40 years old at the moment of their diagnosis. The lesions were composed of fusiform cells arranged in bundles or fascicles. The neoplastic cells were characterized by eosinophilic cytoplasm and tapered nuclei. The presence of vascular spaces was prominent in all cases. The immunocharacteristics of VLOC neoplastic cells were: alpha smooth muscle (+); vimentin (+), desmin (+), CD34 (-) and S-100 protein (-). The endothelial cells of vascular spaces were CD34 (+). Differential diagnosis of VLOC with fusocellular neoplasm is discussed.