Relationship between foliar polycyclic aromatic hydrocarbons (PAHs) concentrations and plant traits: Intracanopy variability for a broadleaf species in an urban environment.

The emission of potentially harmful compounds, including polycyclic aromatic hydrocarbons (PAHs), and the resulting air pollution is a serious problem in modern cities. It is therefore important to develop mitigation strategies, such as "smart" planting of trees that act as sinks for PAHs. However, the intra-individual (within-tree) variability in leaf PAH concentrations remains unknown. In this paper, we studied 15 ornamental apple trees (Malus × moerlandsii 'Profusion') growing on a main street in a medium-sized city in Galicia (NW Spain). We determined the PAH concentrations at 12 canopy positions in each tree (2 orientations and 2 distances from the trunk at 3 heights), measured various ecological traits (specific leaf area [SLA], δ13C, stomatal density, fatty acid contents and leaf hairiness) and analyzed the variability in traits within the canopy in relation to PAH concentrations. We observed high intra-individual variability in the PAH concentrations and the leaf traits. Statistical analyses revealed that leaf height was the main source of variability both in the PAH concentrations and in the traits, mainly due to the leaf morphology, particularly to the SLA. Therefore, the ideal vegetation to remove PAHs would be high leaf biomass trees, not too tall and with a high proportion of shade leaves.

Comparison of microplastic type, size, and composition in atmospheric and foliage samples in an urban scenario.

The rising trend of plastic production in last years and the inadequate disposal of related waste has raised concerns regarding microplastic-related environmental issues. Microplastic particles disperse by means of transport and deposition processes to different ecosystems and enter food chains. In this paper, atmospheric deposition and foliage samples of two species (i.e., Hedera helix and Photinia glabra) were collected and analysed for the quantity and identity of microplastics (MPs). A preliminary methodology to treat foliage samples and subsequently identify MPs using a quantum cascade laser IR spectrophotometer is presented. The treatment of airborne samples involved filtration, mild digestion, concentration, and transfer onto reflective slides whereas that for foliage involved washing, concentration, and transference of putative MPs onto reflective slides. Fibers and fragments were differentiated according to their physical features (size, width, height, etc.) and calculating derived characteristics (namely, circularity and solidity). The preliminary results obtained suggest a good agreement between atmospheric-deposited and foliage-retained MPs, showing the capability of leaves to act as passive samplers for environmental monitoring.

Arsenic movement and fractionation in agricultural soils which received wastewater from an adjacent industrial site for 50 years.

Arsenic (As) is an element with important environmental and human health implications due to its toxic properties. It is naturally occurring since it is contained in minerals, but it can also be enriched and distributed in the environment by anthropogenic activities. This paper reports on the historic As contamination of agricultural soils in one of the most important national relevance site for contamination in Italy, the so-called SIN Brescia-Caffaro, in the city of Brescia, northern Italy. These agricultural areas received As through the use of irrigation waters from wastewater coming from a factory of As-based pesticides (lead and calcium arsenates, sodium arsenite). Pesticide production started in 1920 and ended in the '70. Concentrations in the areas are generally beyond the legal threshold values for different soil uses and are up to >200 mg/kg. Arsenic contamination was studied to assess the long-time trend and the dynamics related to the vertical movement of As down to 1 m depth and its horizontal diffusion with surface irrigation in the entire field. Arsenic fractionation analysis (solid phase speciation by sequential extraction procedure) was also performed on samples collected from these areas and employed in greenhouse experiments with several plant species to evaluate the long-term contamination and the role of plant species in modifying As availability in soil. The results of this work can help in the evaluation of the conditions controlling the vertical transfer of As towards surface aquifers, the bioaccumulation likelihood in the agricultural food chain and the selection of sustainable remediation techniques such as phytoextraction.

Environmental fate of sulfonated-PCBs: Soil partitioning properties, bioaccumulation, persistence, and mobility.

Two new classes of PCB metabolites were recently discovered: sulfonated-polychlorinated biphenyls (sulfonated-PCBs) and hydroxy-sulfonated-polychlorinated biphenyls (OH-sulfonated-PCBs). These metabolites, originating from PCB degradation, seem to possess more polar characteristics than their parent compounds. However, no other information, such as their chemical identity (CAS number) or their ecotoxicity or toxicity, is available so far, although more than about one hundred different chemicals were observed in soil samples. In addition, their physico-chemical properties are still uncertain since only estimations are available. Here we show the first evidence on the fate of these new classes of contaminants in the environment, producing results from several experiments, to evaluate sulfonated-PCBs and OH-sulfonated-PCBs soil partition coefficients, degradation in soil after 18 months of rhizoremediation, uptake into plant roots and earthworms, as well as a preliminary analytical method to extract and concentrate these chemicals from water. The results give an overview of the expected environmental fate of these chemicals and open questions for further studies.

Predicting the contribution of a local emission source in mid-range transport of DDT and its deposition in terrestrial and aquatic ecosystems in Northern Italy.

A recently developed dynamic multiple box multimedia fate model (Gridded-SoilPlusVeg, or GSPV) was developed and implemented to account for the environmental variation and the effect of directional advective transport of chemicals towards different compartments and geographical locations. A chemical plant located in Pieve Vergonte in Ossola Valley produced and emitted DDTs for around 50 years. In the previous study the fate and transport of p,p'-DDT emitted from the chemical plant were evaluated in nearby areas (up to 12 km). In this paper, the GSPV model was run for p,p'-DDT from its production period and decades after the production stop in 1996 (a total of 100 years) for a much larger study area (40,000 km2) in order to evaluate the contribution of a local source on a larger scale. Additionally, the deposition fluxes into the lakes were calculated and were used as input into a dynamic fugacity-based aquatic model to calculate DDT concentration in water and sediments of three Prealpine lakes: Lake Maggiore, Lake Como and Lake Lugano. The results of the simulations were compared with the monitoring and literature data. The results obtained from GSPV allowed to estimate the atmospheric deposition fluxes and identify the role of this source for the regional scale contamination in terrestrial and aquatic ecosystems.

Synthesis of a new sulfonated-hexachlorobiphenyl standard for environmental analysis, ecotoxicological, and toxicological studies.

Sulfonated-polychlorinated biphenyls (sulfonated-PCBs) are a newly discovered class of PCB metabolites. They were observed for the first time in polar bear serum and lately, in soil, together with hydroxy-sulfonated-PCBs. Their presence is ubiquitous in soils, and their estimated physical chemical properties show high mobility in water, compared to the parent compounds. However, no single pure standards exist so far and therefore their quantification in the environmental matrices is not accurate. Additionally, pure standards are needed to experimentally determine their physical chemical properties, as well as the ecotoxicological and toxicological characteristics. In the present work, the challenging goal of preparing a polychlorinated biphenyl monosulfonic acid was achieved exploring different synthetic approaches, along which the selection of the starting material resulted in a crucial point. Using PCB-153 (2,2'-4,4'-5,5'-hexachloro-1,1'-biphenyl) the synthesis afforded, as the major species, a side compound. On the contrary, the use of PCB-155 (2,2'-4,4'-6,6'-hexachloro-1,1'-biphenyl), a symmetric hexachlorobiphenyl derivative showing chlorine atoms at all the ortho positions, gave the target sulfonated-PCB compound. In this case, sulfonation was successfully carried out through a two-step procedure, involving chlorosulfonylation and the subsequent hydrolysis of the chlorosulfonyl intermediate.

Plant-air partition coefficients for thirteen urban conifer tree species: Estimating the best gas and particulate matter associated PAH removers.

Polycyclic Aromatic Hydrocarbons (PAHs) are an important class of pollutants which mostly come out from incomplete combustion of organic materials including fossil fuels. For this reason, they are often found at high concentrations in cities, contaminating air with their gas and particle phase. While European Union policies try to reduce their concentrations, huge efforts are still devoted to mitigate the pollution by PAHs. One such measure of mitigation is the use of plants in capturing PAHs and other chemicals due to the ability of leaves in filtering both gas and particle fractions. In this work thirteen conifer species among those frequently living in temperate and Mediterranean cities were selected and concentrations of 16 PAHs were measured in needles. The data for spruce were used to back-calculate air concentrations of PAHs which were later used to obtain specific equations of plant-air partition coefficient (KPA). The results showed that the difference in total PAH uptake between the most (Pinus pinaster) and the least (Picea abies) efficient tree was close to an order of magnitude. Looking to the capability of the different species in enriching the particulate matter (PM) associated fraction of PAHs on their needles, Pinus pinaster was instead the least important (3.4% of total PAHs), while Cupressus lusitanica was the most important (34% of total PAHs). The new KPA equations can be used to fine tune the PAH uptake of a specific amount of plant biomass on air concentration reduction.

Predicting the regional contamination evolution of DDT for 100-years with a new gridded spatial and dynamic multimedia fate model.

In 1996 high dichlorodiphenyltrichloroethane (DDT) concentrations were found in Lake Maggiore (Italy) fish and sediments. DDT was produced by a chemical company located in a subalpine valley (Ossola valley, Piedmont Region, Italy), and ended up in the Toce River, a tributary of Lake Maggiore. In the area surrounding the chemical plant, high DDT concentrations in soil and vegetation were found after subsequent investigations. The quantification of the release from contaminated soil and the following migration toward downwind areas, deposition to the soil, and further evaporation plays an important role in understanding future DDT trends in soil and the atmosphere. To study this phenomenon, soil, and vegetation from Ossola Valley were monitored in 2001 and 2011. The concentration values obtained (soils: 0.05 to 1 µg/g; vegetation 2-100 ng/g), allowed to reconstruct the contamination gradient in the valley and were used to develop and calibrate a spatially resolved multimedia fugacity model. The model accounts for spatial and temporal dynamicity of environmental characteristics such as wind speed and direction, variable air compartment height etc., and simulates the fate and transport of chemicals on a local scale. The dynamic emission of DDT (about 13,000 kg for the 50 y production time) to the air was estimated and utilized for a 100-year simulation (from 1948 to 2048). The results obtained allowed to understand the temporal and spatial pattern of DDT contamination for a long period at a local scale as well as the potential contribution as a source potentially affecting sites at larger distances.

Pollution and edaphic factors shape bacterial community structure and functionality in historically contaminated soils.

Studies about biodegradation potential in soils often refer to artificially contaminated and simplified systems, overlooking the complexity associated with contaminated sites in a real context. This work aims to provide a holistic view on microbiome assembly and functional diversity in the model site SIN Brescia-Caffaro (Italy), characterized by historical and uneven contamination by organic and inorganic compounds. Here, physical and chemical analyses and microbiota characterization were applied on one-hundred-twenty-seven soil samples to unravel the environmental factors driving bacterial community assembly and biodegradation potential in three former agricultural fields. Chemical analyses showed a patchy distribution of metals, metalloids and polychlorinated biphenyls (PCB) and allowed soil categorization according to depth and area of collections. Likewise, the bacterial community structure, described by molecular fingerprinting and 16S rRNA gene analyses, was significantly different according to collection site and depth. Pollutant concentrations (i.e., hexachloro-biphenyls, arsenic and mercury), nitrogen content and parameters related to soil texture were identified as main drivers of microbiota assembly, being significantly correlated to bacterial community composition. Moreover, bacteria putatively involved in the aerobic degradation of PCBs were enriched over the total bacterial community in topsoils, where the highest activity was recorded using fluorescein hydrolysis as proxy. Metataxonomic analyses revealed the presence of bacteria having metabolic pathways related to PCB degradation and tolerance to heavy metals and metalloids in the topsoil samples collected in all areas. Overall, the provided dissection of soil microbiota structure and its degradation potential in the SIN Brescia-Caffaro can contribute to target specific areas for rhizoremediation implementation. Metagenomics studies could be implemented in the future to understand if specific degradative pathways are present in historically polluted sites characterized by the co-occurrence of multiple classes of contaminants.

Life cycle exposure of plants considerably affects root uptake of PCBs: Role of growth strategies and dissolved/particulate organic carbon variability.

Plant roots can accumulate organic chemicals, including PCBs, and this could be relevant in spreading chemicals through the food chain. To estimate such uptake, several equations are available in the literature, mostly developed in lab conditions, to obtain the root concentration factor (RCF). Here, a long-term (18 months) greenhouse experiment, using an aged, contaminated soil, was performed to reproduce root uptake in field-like conditions and to account for the ecological variability of exposure during the entire life cycle. Specific growth strategies (i.e., annual vs. perennial), root development (e.g., timing of root production and decaying), and soil parameters (e.g., dissolved organic carbon (DOC), and the particulate organic carbon (POC)) may interfere with the uptake of contaminants into the roots of plants. In this study, we investigate the effects of these factors on the RCF, obtained for 79 PCBs. New predictive equations were calculated for 5 different plants species at four different growth times (from few months to 1.5 years) and stages (growing vs maturity). The relationships highlighted a species-specific and time-dependent accumulation of PCB in plants roots, with higher RCFs in summer than in fall for some species, and the relevant influence of DOC and POC in affecting root uptake.

Bioaccumulation of PCBs and their hydroxy and sulfonated metabolites in earthworms: Comparing lab and field results.

Sulfonated and hydroxy-sulfonated PCBs were recently discovered by our group as new PCB soil contaminants, constituting about 1% of their parent compounds in soil. Here we investigate for the first time the bioaccumulation of these metabolites as well as hydroxy-PCBs and native PCBs in earthworms. A sequence of three experiments, at increasing complexity and ecological realism, were performed with four different earthworm species (Eisenia foetida Savigny, Lumbricus terrestris L, Allolobophora chlorotica Savigny and Aporrectodea caliginosa Savigny) exposed to contaminated soils. The first experiment confirmed that when exposing earthworms to soil contaminated with a single hexa-chlorinated congener (PCB 155), no formation of polar metabolites in earthworms could be detected. This allowed to plan the following two experiments, using a soil from a PCB contaminated site and rich in relatively high levels (10-130 µg kg-1) of hydroxy-, sulfonated-, and hydroxy-sulfonated-PCBs. Bioaccumulation factors (BAFs) and bioconcentration factors (BCFs) were then obtained in the second and third experiments, to compare the accumulation behavior of these chemicals in laboratory and natural conditions. Regressions between BAF/BCF and Log Kow/Log D, produced a variety of results, being generally significant between BCF and PCBs and not significant in the other cases. In general, the metabolites accumulated in earthworms with detectable concentrations in their tissues (8-115 µg kg-1), although sulfonated and hydroxy-sulfonated PCBs showed BAF and BCF values lower (up to two orders of magnitude) than those calculated for the parent PCBs, given their lower lipophilicity.

Spatially resolved environmental fate models: A review.

Spatially resolved environmental models are important tools to introduce and highlight the spatial variability of the real world into modeling. Although various spatial models have been developed so far, yet the development and evaluation of these models remain a challenging task due to several difficulties related to model setup, computational cost, and obtaining high-resolution input data (e.g., monitoring and emission data). For example, atmospheric transport models can be used when high resolution predicted concentrations in atmospheric compartments are required, while spatial multimedia fate models may be preferred for regulatory risk assessment, life cycle impact assessment of chemicals, or when the partitioning of chemical substances in a multimedia environment is considered. The goal of this paper is to review and compare different spatially resolved environmental models, according to their spatial, temporal and chemical domains, with a closer insight into spatial multimedia fate models, to achieve a better understanding of their strengths and limitations. This review also points out several requirements for further improvement of existing models as well as for their integration.

Mercury vertical and horizontal concentrations in agricultural soils of a historically contaminated site: Role of soil properties, chemical loading, and cultivated plant species in driving its mobility.

The long term vertical and horizontal mobility of mercury (Hg) in soils of agricultural areas of a historically contaminated Italian National Relevance Site (SIN Brescia-Caffaro) was investigated. The contamination resulted from the continuous discharge of Hg in irrigation waters by an industrial plant (Caffaro S.p.A), equipped with a mercury-cell chlor-alkali process. The contamination levels with depth ranged from about 20 mg/kg dry weight (d.w.) of soil in the top (plow) layer to less than 0.1 mg/kg d.w. at 1 m depth. The concentrations varied also spatially, up to one order of magnitude within the same field and showing a decreasing trend from the Hg source (i.e., irrigation ditches). The concentration profiles and gradients measured were explained considering Hg loading, soil properties, such as the texture, organic carbon content, pH and cation exchange capacity. A Selective Sequential Extraction (SSE) was also applied on soil samples from an ad hoc greenhouse experiment to investigate the role of different plant species in influencing Hg speciation in soils. Although most of the extracted Hg was included in scarcely mobile or immobile forms, some plant species (i.e., alfalfa) showed to importantly increase the soluble and exchangeable fractions with respect to the unplanted control soils, thus affecting mobility and potential bioavailability of Hg.

Microbial degradation of pyrene in holm oak (Quercus ilex) phyllosphere: Role of particulate matter in regulating bioaccessibility.

In this study we first measured the mineralization of pyrene on leaves of urban holm oak (Quercus ilex) by autochthonous microorganisms and an inoculated PAH degrading bacterium (i.e., Mycobacterium gilvum), selected as a model phyllosphere species, as well as the leaf-water (KLW) and leaf-air (KLA) partition coefficients for this chemical. Mineralization was investigated in two different experimental systems in terms of leaf and microorganism environment. Additionally, the influence on pyrene partitioning and mineralization when particulate matter (PM) was present on the leaf surface or removed was studied. Mineralization of 14C-labeled pyrene by autochthonous microorganisms was lower than 1% after approximately two weeks, while M. gilvum mineralized 5% to 17% of pyrene. These extents corresponded to mineralization half-lives that ranged between ~30 to ~200 days. We proposed that PM present at the leaf surface reduced the accumulation of pyrene by inner compartments (cuticle) distantly located from microbial cells and enhanced the bioaccessibility of pyrene, speeding up microbial activity and therefore mineralization. These results highlight that plant-phyllosphere microorganism interaction is more complex than currently established and deserves additional studies to further comprehend the air purification ecosystem service of phyllosphere microorganisms.

Estimating temporal and spatial levels of PAHs in air using rain samples and SPME analysis: Feasibility evaluation in an urban scenario.

There is a growing interest in evaluating the role of concentration changes of contaminants in temporal and spatial gradients. This is often relevant for fast moving environmental phases such as air and water. In this paper, small volumes of rainwater were sampled as proxy for air concentrations of Polycyclic Aromatic Hydrocarbons (PAHs): rain was collected in three sampling sites (high traffic, restricted traffic and a low traffic zone) in Como. Solid phase micro extraction (SPME) was used for the extraction to reduce required sample volumes, allowing the acquisition of more samples in time. Rain samples highlighted a spatial and temporal variability along a traffic gradient in the Como city, especially for the most abundant PAH, e.g. phenanthrene. Air concentrations were then estimated from rain concentrations. The results show that this is a cheap and promising method, although requiring rainfall/snowfall conditions, that can be used to perform monitoring campaign of air concentrations at a higher temporal and spatial resolution than the adopted standard methods (e.g. high-volume air samplers). The results could be employed for evaluation of the exposure, emission profiles and calibration of fate models.

A new dataset of PCB half-lives in soil: Effect of plant species and organic carbon addition on biodegradation rates in a weathered contaminated soil.

This paper presents a new dataset of Polychlorinated Biphenyls (PCBs) half-lives in soil. Data were obtained from a greenhouse experiment performed with an aged contaminated soil under semi-field conditions, collected from a National Relevance Site (SIN) located in Northern Italy (SIN Brescia-Caffaro). Ten different treatments (combination of seven plant species and different soil conditions) were considered together with the respective controls (soil without plants). PCB concentration reduction in soil was measured over a period of 18 months to evaluate the ability of plants to stimulate the biodegradation of these compounds. Tall fescue, tall fescue cultivated together with pumpkin and tall fescue amended with compost reduced more than the 50% of the 79 measured PCB congeners, including the most chlorinated ones (octa to deca-PCBs). However, the data obtained showed that no plant species was uniquely responsible for the effective degradation of all isomeric classes and congeners. The obtained half-lives ranged from 1.3 to 5.6 years and were up to a factor of 8 lower compared to generic HL values reported in literature. This highlighted the importance of cultivation and plant-microbe interactions in speeding up the PCB biodegradation. This new dataset could contribute to substantially improve the predictions of soil remediation time, multimedia fate and the long-range transport of PCBs. Additionally, the half-lives obtained here can also be used in the evaluation of the food chain transfer of these chemicals, and finally the exposure and potential for effects on ecosystems.

PCB vertical and horizontal movement in agricultural soils of a highly contaminated site: Role of soil properties, cultivation history and PCB physico-chemical parameters.

The agricultural areas of a historically contaminated National Relevance Site (SIN Brescia Caffaro) in Italy are an ideal case for studying the long term vertical and horizontal movement of polychlorinated biphenyls (PCBs) in soil. Here, a former large producer of PCBs (Caffaro S.p.A.) discharged its wastewaters, contaminated by PCBs and other chemicals, to a ditch used for about 80 years as source of irrigation waters for the adjacent agricultural areas. This caused a spread of contamination along both a vertical and a horizontal soil gradient. PCB concentrations of about 80 congeners, including PCB 209, peculiar of Caffaro production, were measured in three areas, selected for their different soil properties and cultivation history. The contamination levels with depth ranged from about 30 mg/kg dry weight (d.w.) of soil in the top (plow) layer to less than 0.1 mg/kg d.w. at the depth of 1 m. The concentrations varied also horizontally, since each field was surface irrigated from the short edge of each field, showing that PCBs could spread with length halving the initial concentrations in the topsoil only after about 30-35 m. The concentration gradients detected were explained considering the historic soil use and its change with time, the pedological properties as well as PCB physico-chemical parameters and halflives, developing equations which could be employed as guidance tools for evaluating PCBs (and similar chemicals) movement and direct further studies.

New Data Set of Polychlorinated Dibenzo-p-dioxin and Dibenzofuran Half-Lives: Natural Attenuation and Rhizoremediation Using Several Common Plant Species in a Weathered Contaminated Soil.

In this paper, a new data set of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/Fs) half-lives (HLs) in soil is presented. Data are derived from a greenhouse experiment performed with an aged contaminated soil under semi-field conditions, obtained from a National Relevance Site (SIN) located in Northern Italy (SIN Brescia-Caffaro). Ten different treatments (combination of seven plant species with different soil conditions) were considered together with the respective controls (soil without plants). The ability of the plants to stimulate the biodegradation of these compounds was evaluated by measuring the PCDD/F concentration reduction in soil over a period of 18 months. The formation of new bound residues was excluded by using roots as a passive sampler of bioaccessible concentrations. The best treatment which significantly reduced PCDD/F concentrations in soil was the one with Festuca arundinacea (about 11-24% reduction, depending on the congener). These decreases reflected in HLs ranging from 2.5 to 5.8 years. Simulations performed with a dynamic air-vegetation-soil model (SoilPlusVeg) confirmed that these HLs were substantially due to biodegradation rather than other loss processes. Because no coherent PCDD/F degradation HL data sets are currently available for soil, they could substantially improve the predictions of soil remediation time, long-range transport, and food chain transfer of these chemicals using multimedia fate models.

Role of photo- and biodegradation of two PAHs on leaves: Modelling the impact on air quality ecosystem services provided by urban trees.

Urban trees provide important ecosystem services, including air quality improvement. Polycyclic aromatic hydrocarbons (PAHs) are among the most important pollutants in air, due to their elevated concentrations and toxicity. Plants can act as filters of PAHs and as "chemical reactors" for pollutant removal, therefore reducing air concentrations. Here, the first assessment of photo- vs. biodegradation of PAHs on leaves of urban trees is presented. A dynamic air-vegetation-soil model (SoilPlusVeg) was improved to simulate the fate of two representative PAHs with contrasting physico-chemical properties (phenanthrene and benzo[a]pyrene). Simulations were performed for two different environmental scenarios from Italy (Como and Naples), selected for their dissimilar meteorological parameters, plant species and emission levels. The effect of photo- and biodegradation on leaf concentrations and fluxes towards air and soil was investigated comparing deciduous (maple, cornel and hazelnut) and evergreen (holm oak) broadleaf woods. The results showed that biodegradation in the phyllosphere could not be neglected when evaluating the ecosystem services provided by urban trees, as this process contributed significantly to the reductions (up to 25% on average) in PAH leaf concentrations and fluxes to air and soil; however, the reductions revealed ample variations with time (up to more than two orders of magnitude) showing the dependence on meteorological parameters, air compartment structure, as well as type of woods. These findings permitted to improve the ecological realism of the simulations and obtain more accurate results when predicting organic contaminant uptake and release by plant leaves, including potential for food chain transfer and long-range transport.

Plants radically change the mobility of PCBs in soil: Role of different species and soil conditions.

The mobility of Polychlorinated Biphenyls (PCBs) in soil cultivated with different plant species was evaluated by means of a column experiment to investigate the specific plant influence on PCB environmental fate and the potential for leaching. The soil was collected at a National Relevance Site for remediation located in Northern Italy (SIN Brescia-Caffaro) and underwent a rhizoremediation treatment for 18 months with different plant species (Festuca arundinacea, Cucurbita pepo ssp pepo and Medicago sativa). The same but unplanted soil was also considered as control for comparison. The columns were leached with tap water and PCB concentrations were measured in the leachate after 7 days of soil/water contact. Soil previously cultivated with different plant species exhibited statistically different behavior in terms of chemical leaching among the different fractions. Total PCB bulk concentrations ranged from 24 to 219 ng/L. Leachate samples were enriched in tetra- to hepta-PCBs. While PCB concentrations in the dissolved phases varied within a factor of 2 between controls and treatments, PCB associated to particulate organic carbon (POC) differed by more than one order of magnitude. More specifically, Medicago sativa enriched the soil with POC doubling PCB leaching with respect to the other plant species and the unplanted controls.