Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics.

Emergent physical properties of tissues are not readily understood by reductionist studies of their constituent cells. Here, we show molecular signals controlling cellular, physical, and structural properties and collectively determine tissue mechanics of lymph nodes, an immunologically relevant adult tissue. Lymph nodes paradoxically maintain robust tissue architecture in homeostasis yet are continually poised for extensive expansion upon immune challenge. We find that in murine models of immune challenge, cytoskeletal mechanics of a cellular meshwork of fibroblasts determine tissue tension independently of extracellular matrix scaffolds. We determine that C-type lectin-like receptor 2 (CLEC-2)-podoplanin signaling regulates the cell surface mechanics of fibroblasts, providing a mechanically sensitive pathway to regulate lymph node remodeling. Perturbation of fibroblast mechanics through genetic deletion of podoplanin attenuates T cell activation. We find that increased tissue tension through the fibroblastic stromal meshwork is required to trigger the initiation of fibroblast proliferation and restore homeostatic cellular ratios and tissue structure through lymph node expansion.

Junctional and cytoplasmic contributions in wound healing.

Wound healing is characterized by the re-epitheliation of a tissue through the activation of contractile forces concentrated mainly at the wound edge. While the formation of an actin purse string has been identified as one of the main mechanisms, far less is known about the effects of the viscoelastic properties of the surrounding cells, and the different contribution of the junctional and cytoplasmic contractilities. In this paper, we simulate the wound healing process, resorting to a hybrid vertex model that includes cell boundary and cytoplasmic contractilities explicitly, together with a differentiated viscoelastic rheology based on an adaptive rest-length. From experimental measurements of the recoil and closure phases of wounds in the Drosophila wing disc epithelium, we fit tissue viscoelastic properties. We then analyse in terms of closure rate and energy requirements the contributions of junctional and cytoplasmic contractilities. Our results suggest that reduction of junctional stiffness rather than cytoplasmic stiffness has a more pronounced effect on shortening closure times, and that intercalation rate has a minor effect on the stored energy, but contributes significantly to shortening the healing duration, mostly in the later stages.

Development of a New 3D Hybrid Model for Epithelia Morphogenesis.

Many epithelial developmental processes like cell migration and spreading, cell sorting, or T1 transitions can be described as planar deformations. As such, they can be studied using two-dimensional tools and vertex models that can properly predict collective dynamics. However, many other epithelial shape changes are characterized by out-of-plane mechanics and three-dimensional effects, such as bending, cell extrusion, delamination, or invagination. Furthermore, during planar cell dynamics or tissue repair in monolayers, spatial intercalation between the apical and basal sides has even been detected. Motivated by this lack of symmetry with respect to the midsurface, we here present a 3D hybrid model that allows us to model differential contractility at the apical, basal or lateral sides. We use the model to study the effects on wound closure of solely apical or lateral contractile contributions and show that an apical purse-string can be sufficient for full closure when it is accompanied by volume preservation.

EpiGraph: an open-source platform to quantify epithelial organization.

SUMMARY: Here we present EpiGraph, an image analysis tool that quantifies epithelial organization. Our method combines computational geometry and graph theory to measure the degree of order of any packed tissue. EpiGraph goes beyond the traditional polygon distribution analysis, capturing other organizational traits that improve the characterization of epithelia. EpiGraph can objectively compare the rearrangements of epithelial cells during development and homeostasis to quantify how the global ensemble is affected. Importantly, it has been implemented in the open-access platform Fiji. This makes EpiGraph very user friendly, with no programming skills required. AVAILABILITY AND IMPLEMENTATION: EpiGraph is available at https://imagej.net/EpiGraph and the code is accessible (https://github.com/ComplexOrganizationOfLivingMatter/Epigraph) under GPLv3 license. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Tissue Fluidity Promotes Epithelial Wound Healing.

The collective behaviour of cells in epithelial tissues is dependent on their mechanical properties. However, the contribution of tissue mechanics to wound healing in vivo remains poorly understood. Here we investigate the relationship between tissue mechanics and wound healing in live Drosophila wing imaginal discs and show that by tuning epithelial cell junctional tension, we can systematically alter the rate of wound healing. Coincident with the contraction of an actomyosin purse string, we observe cells flowing past each other at the wound edge by intercalating, reminiscent of molecules in a fluid, resulting in seamless wound closure. Using a cell-based physical model, we predict that a reduction in junctional tension fluidises the tissue through an increase in intercalation rate and corresponding reduction in bulk viscosity, in the manner of an unjamming transition. The resultant fluidisation of the tissue accelerates wound healing. Accordingly, when we experimentally reduce tissue tension in wing discs, intercalation rate increases and wounds repair in less time.

The same but different: cell intercalation as a driver of tissue deformation and fluidity.

The ability of cells to exchange neighbours, termed intercalation, is a key feature of epithelial tissues. Intercalation is predominantly associated with tissue deformations that drive morphogenesis. More recently, however, intercalation that is not associated with large-scale tissue deformations has been described both during animal development and in mature epithelial tissues. This latter form of intercalation appears to contribute to an emerging phenomenon that we refer to as tissue fluidity-the ability of cells to exchange neighbours without changing the overall dimensions of the tissue. Here, we discuss the contribution of junctional dynamics to intercalation governing both morphogenesis and tissue fluidity. In particular, we focus on the relative roles of junctional contractility and cell-cell adhesion as the driving forces behind intercalation. These two contributors to junctional mechanics can be used to simulate cellular intercalation in mechanical computational models, to test how junctional cell behaviours might regulate tissue fluidity and contribute to the maintenance of tissue integrity and the onset of disease.This article is part of the Theo Murphy meeting issue 'Mechanics of development'.

Unipolar distributions of junctional Myosin II identify cell stripe boundaries that drive cell intercalation throughout Drosophila axis extension.

Convergence and extension movements elongate tissues during development. Drosophila germ-band extension (GBE) is one example, which requires active cell rearrangements driven by Myosin II planar polarisation. Here, we develop novel computational methods to analyse the spatiotemporal dynamics of Myosin II during GBE, at the scale of the tissue. We show that initial Myosin II bipolar cell polarization gives way to unipolar enrichment at parasegmental boundaries and two further boundaries within each parasegment, concomitant with a doubling of cell number as the tissue elongates. These boundaries are the primary sites of cell intercalation, behaving as mechanical barriers and providing a mechanism for how cells remain ordered during GBE. Enrichment at parasegment boundaries during GBE is independent of Wingless signaling, suggesting pair-rule gene control. Our results are consistent with recent work showing that a combinatorial code of Toll-like receptors downstream of pair-rule genes contributes to Myosin II polarization via local cell-cell interactions. We propose an updated cell-cell interaction model for Myosin II polarization that we tested in a vertex-based simulation.