1.
Angew Chem Int Ed Engl
; 37(23): 3300-3303, 1998 Dec 17.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29711416
RESUMO
By activation of the hydrogen acceptor, the metal-free hydrogenase from methanogenic archaea catalyzes the reduction of methenyl tetrahydromethanopterin with H2 . According to NMR spectroscopic analysis of the conformation of the hydrogen acceptor in solution and of the stereospecificity of the catalyzed and noncatalyzed reaction, in the enzyme-catalyzed reaction the hydrogenation product is formed in a constraint conformation which relaxes upon dissociation from the enzyme. This exergonic conformational change could help to avoid product inhibition of the enzyme.