Tribological Properties of a Sliding Joint with an a-C:H:W Coating under Lubrication Conditions with PAO8 Oil and the Addition of 2% MoS2 Nanoparticles.

One of the promising methods for improving the durability and reliability of friction joints in combustion engines is the use of thin and hard coatings, including coatings based on amorphous DLC. The a-C:H:W coating was produced using the commercial PVD method. The tested tribological joints were made of AISI 4337 steel and SAE-48 bearing alloy (conformal contact) and AISI 4337 steel and valve shims (non-conformal contact). The contact area was lubricated with SAE 5W40 engine oil and PAO8 oil + 2 wt.% MoS2 nanoparticles. The objective of this work is to explore the influence of PAO8 + MoS2 on the tribological properties of a sliding joint with an a-C:H:W coating and the change in the properties of the oils. In the conformal contact, the lubrication of the a-C:H:W coating with PAO8 + MoS2 caused a significant increase in the friction resistance (than in) as compared to the joints with a quenching and tempering surface layer and lubricated SAE 5W40, while in the non-conformal contact, the lubrication of the a-C:H:W coating with PAO8 + MoS2 caused a decrease in the friction resistance and temperature of the contact area. The joints with the a-C:H:W coating were characterized by higher wear of the SAE-48 bearing alloy, as compared to the joints with the surface layer without coating (lubricated with SAE 5W40 oil-11-fold increase, PAO8 + MoS2-46-fold increase). The wear of valve shims with the a-C:H:W coating was significantly lower as compared to the wear of the commercial version of the valve shims (the difference between joints lubricated with SAE 5W40 oil and PAO8 + MoS2 was 12%, 36% and 29% for unit pressures of 10, 15 and 20 MPa). Lubrication of the a-C:H:W coating with PAO8 oil + MoS2 protected the sliding joints against seizing in non-conformal contact.

Bioactive Hydrogel Based on Collagen and Hyaluronic Acid Enriched with Freeze-Dried Sheep Placenta for Wound Healing Support.

In an increasingly aging society, there is a growing demand for the development of technology related to tissue regeneration. It involves the development of the appropriate biomaterials whose properties will allow the desired biological response to be obtained. Bioactivity is strongly affected by the proper selection of active ingredients. The aim of this study was to produce bioactive hydrogel materials based on hyaluronic acid and collagen modified by the addition of placenta. These materials were intended for use as dressings, and their physicochemical properties were investigated under simulated biological environmental conditions. The materials were incubated in vitro in different fluids simulating the environment of the human body (e.g., simulated body fluid) and then stored at a temperature close to body temperature. Using an FT-IR spectrophotometer, the functional groups present in the composites were identified. The materials with the added placenta showed an increase in the swelling factor of more than 300%. The results obtained confirmed the potential of using this material as an absorbent dressing. This was indicated by pH and conductometric measurements, sorption, degradation, and surface analysis under an optical microscope. The results of the in vitro biological evaluation confirmed the cytosafety of the tested biomaterials. The tested composites activate monocytes, which may indicate their beneficial properties in the first phases of wound healing. The material proved to be nontoxic and has potential for medical use.

Clindamycin-Loaded Nanosized Calcium Phosphates Powders as a Carrier of Active Substances.

Bioactive calcium phosphate ceramics (CaPs) are one of the building components of the inorganic part of bones. Synthetic CaPs are frequently used as materials for filling bone defects in the form of pastes or composites; however, their porous structure allows modification with active substances and, thus, subsequent use as a drug carrier for the controlled release of active substances. In this study, four different ceramic powders were compared: commercial hydroxyapatite (HA), TCP, brushite, as well as HA obtained by wet precipitation methods. The ceramic powders were subjected to physicochemical analysis, including FTIR, XRD, and determination of Ca/P molar ratio or porosity. These techniques confirmed that the materials were phase-pure, and the molar ratios of calcium and phosphorus elements were in accordance with the literature. This confirmed the validity of the selected synthesis methods. CaPs were then modified with the antibiotic clindamycin. Drug release was determined on HPLC, and antimicrobial properties were tested against Staphylococcus aureus. The specific surface area of the ceramic has been demonstrated to be a factor in drug release efficiency.

Evaluation of the impact of pH of the reaction mixture, type of the stirring, and the reagents' concentration in the wet precipitation method on physicochemical properties of hydroxyapatite so as to enhance its biomedical application potential.

Hydroxyapatite (HAp) constitutes a significant inorganic compound which due to its osteoinductivity, osteoconductivity as well as the ability to promote bone growth and regeneration is widely applied in development of biomaterials designed for bone tissue engineering. In this work, various synthesis methodologies of HAp based on the wet precipitation technique were applied, and the impact of pH of the reaction mixture, the concentration of individual reagents as well as the type of stirring applied (mechanical/magnetic) on the properties of final powders was discussed. Spectroscopic methods (Fourier transform infrared, Raman) and X-ray diffraction allowed to verify the synthesis parameters leading to obtaining calcium phosphate with 96% HAp in phase which indicated higher homogeneity of obtained powder (93.4%) than commercial HAp. Powders' morphology was evaluated using microscopic techniques while specific surface area was determined via Brunauer-Emmett-Teller analysis. Particle size distribution, porosity of powders, and stability of HAp suspensions were also characterized. It was proved that synthesis at pH = 11.0 using mechanical stirring resulted in calcium phosphate with a high phase homogeneity and homogeneous pore size distribution (6-20 nm). Moreover, obtained HAp powder showed 71.8% more specific surface area than commercial material-that is, 110 m3 /g for synthetic HAp and 64 m3 /g in the case of commercial powder-which, in turn, is significant in terms of its potential application as carrier of active substances. Thus it was demonstrated that by applying appropriate conditions of HAp synthesis it is possible to obtain powder with properties enhancing its application potential for medical purposes.

Review of the Applications of Biomedical Compositions Containing Hydroxyapatite and Collagen Modified by Bioactive Components.

Regenerative medicine is becoming a rapidly evolving technique in today's biomedical progress scenario. Scientists around the world suggest the use of naturally synthesized biomaterials to repair and heal damaged cells. Hydroxyapatite (HAp) has the potential to replace drugs in biomedical engineering and regenerative drugs. HAp is easily biodegradable, biocompatible, and correlated with macromolecules, which facilitates their incorporation into inorganic materials. This review article provides extensive knowledge on HAp and collagen-containing compositions modified with drugs, bioactive components, metals, and selected nanoparticles. Such compositions consisting of HAp and collagen modified with various additives are used in a variety of biomedical applications such as bone tissue engineering, vascular transplantation, cartilage, and other implantable biomedical devices.

Investigations on the Influence of Collagen Type on Physicochemical Properties of PVP/PVA Composites Enriched with Hydroxyapatite Developed for Biomedical Applications.

Nowadays, a great attention is directed into development of innovative multifunctional composites which may support bone tissue regeneration. This may be achieved by combining collagen and hydroxyapatite showing bioactivity, osteoconductivity and osteoinductivity with such biocompatible polymers as polyvinylpyrrolidone (PVP) and poly(vinyl alcohol) (PVA). Here PVA/PVP-based composites modified with hydroxyapatite (HAp, 10 wt.%) and collagen (30 wt.%) were obtained via UV radiation while two types of collagen were used (fish and bovine) and crosslinking agents differing in the average molecular weight. Next, their chemical structure was characterized using Fourier transform infrared (FT-IR) spectroscopy, roughness of their surfaces was determined using a stylus contact profilometer while their wettability was evaluated by a sessile drop method followed by the measurements of their surface free energy. Subsequently, swelling properties of composites were verified in simulated physiological liquids as well as the behavior of composites in these liquids by pH measurements. It was proved that collagen-modified composites showed higher swelling ability (even 25% more) compared to unmodified ones, surface roughness, biocompatibility towards simulated physiological liquids and hydrophilicity (contact angles lower than 90°). Considering physicochemical properties of developed materials and a possibility of the preparation of their various shapes and sizes, it may be concluded that developed materials showed great application potential for biomedical use, e.g., as materials filling bone defects supporting their treatments and promoting bone tissue regeneration due to the presence of hydroxyapatite with osteoinductive and osteoconductive properties.

In situ tribochemical sulfurization of molybdenum oxide nanotubes.

MoS2 nanoparticles are typically obtained by high temperature sulfurization of organic and inorganic precursors under a S rich atmosphere and have excellent friction reduction properties. We present a novel approach for making the sulfurization unnecessary for MoO3 nanotubes during the synthesis process for friction and wear reduction applications while simultaneously achieving a superb tribological performance. To this end, we report the first in situ sulfurization of MoO3 nanotubes during sliding contact in the presence of sulfur-containing lubricant additives. The sulfurization leads to the tribo-chemical formation of a MoS2-rich low-friction tribofilm as verified using Raman spectroscopy and can be achieved both during sliding contact and under extreme pressure conditions. Under sliding contact conditions, MoO3 nanotubes in synergy with sulfurized olefin polysulfide and pre-formed zinc dialkyl dithiophosphate tribofilms achieve an excellent friction performance. Under these conditions, the tribochemical sulfurization of MoO3 nanotubes leads to a similar coefficient of friction to the one obtained using a model nanolubricant containing MoS2 nanotubes. Under extreme pressure conditions, the in situ sulfurization of MoO3 nanotubes using sulfurized olefin polysulfide results in a superb load carrying capacity capable of outperforming MoS2 nanotubes. The reason is that while MoO3 nanotubes are able to continuously sulfurize during sliding contact conditions, MoS2 nanotubes progressively degrade by oxidation thus losing lubricity.

Ventilator-associated pneumonia monitoring according to the INICC project at one centre.

BACKGROUND: Pneumonia is a common complication of hospitalisation in severely ill patients who need mechanical ventilation. The aim of this study was to assess the usefulness of the International Nosocomial Infection Control Consortium programme for the surveillance of ventilator-associated pneumonia (VAP). METHODS: A prospective study (1 Jan 2012-30 June 2014) was conducted in the 20-bed ICU. The device utilisation ratios for lung ventilation and the frequency (density and incidence) and aetiology of VAP were estimated in ICU patients. RESULTS: From a total of 1097 patients, VAP infections were diagnosed in 93. Thirty percent of patients with VAP died. The incidence index was 8.47 per 100 admissions to the ICU. VAP infections accounted for 46% of the overall count of device-associated healthcare-associated infections. Mechanical ventilation was used in 71 ± 8 patients during the 11 862 patient days and 8425 ventilation days. The rate of VAP per 1000 ventilator days was 11.15/9.34 /10.23 in years 2012/2013/2014 (half a year), respectively. The main VAP pathogens were Acinetobacter baumannii (45%) and Pseudomonas aeruginosa (17%). CONCLUSION: During the reported time span, the incidence of VAP was lower than in the INICC report (2007-2012), but it was tenfold higher than in the NHSN/CDC report (dated 2012). Because of the unchanged VAP level during the 2.5-year observation period, the root cause needs to be determined and action should be taken to resolve this issue.