The effect of local application of thymoquinone, Nigella sativa's bioactive component, on bone healing in experimental bone defects infected with Porphyromonas gingivalis.

PURPOSE: This study was performed to evaluate the influence of local application of thymoquinone (TQ) on bone healing in experimental bone defects infected with Porphyromonas gingivalis (PG). METHODS: Forty-two female rats were randomly divided into 6 groups. A bone defect was created on the right tibia of all animals. The PG, PG/collagen membrane (COL) and PG/TQ/COL groups were infected with PG. In the COL and PG/COL groups, the defects were covered with a COL; in the TQ/COL and PG/TQ/COL groups, the defects were covered with a TQ-containing COL. After 28 days, all animals were sacrificed. Quantitative measurements of new bone formation and osteoblast lining, as well as semiquantitative measurements of capillary density and tissue response, were analyzed. Furthermore, the presence of bacterial infections in defect areas was evaluated. RESULTS: The new bone formation, osteoblast number, and capillary density were significantly higher in the TQ groups than in the control groups (P<0.001, P<0.001, and P<0.01, respectively). In a comparison between the TQ/COL group, with a TQ-containing COL (TQ/COL), and the PG-infected TQ-containing COL (PG/TQ/COL) group, the newly formed bone and capillary density were higher in the TQ/COL group (P<0.01). When the control group was compared to the PG, PG/COL, and PG/TQ/COL groups in terms of tissue response, the differences were statistically significant (P<0.001, P=0.02, and P=0.041, respectively). The intensity of the inflammatory cell reaction was higher in the PG, PG/COL, and PG/TQ/COL groups (P<0.05). CONCLUSIONS: Within the limitations of this study, the local application of a TQ-containing COL positively affected bone healing even if the bone defects were infected. The results suggest that TQ increased angiogenesis and showed promise for accelerating bone defect healing. Further research is warranted to support these findings and reach more definitive conclusions.

Acoustic diagnosis of elastic properties of human tooth by 320 MHz scanning acoustic microscopy after radiotherapy treatment for head and neck cancer.

BACKGROUND: On the elastic profiles of human teeth after radiotherapy for head and neck cancers, generation of dental complications, which may bring several side effects preventing the quality of life, has not well clarified. Thus, we aimed to show the applicability of using 320 MHz Scanning Acoustic Microscopy (SAM) in the evaluation of the tooth damage acoustically at the micrometer level following radiation therapy, and also in the determination of the safe dose limits to impede severe dental damage. METHODS: This prospective study was performed by SAM employed at 320 MHz by an azimuthal resolution of 4.7 µm resolving enamel and dentin. A total of 45 sound human third molar teeth collected between September 2018 and May 2019 were used for the acoustic impedance measurements pre- and post irradiation. Nine samples for each group (control, 2 Gy, 8 Gy, 20 Gy, 30 Gy and 60 Gy) were evaluated to acquire the acoustic images and perform a qualitative analysis. Scanning Electron Microscopy (SEM) images were obtained to establish a relationship between micromechanical and morphological characteristics of the teeth. Statistical analysis was conducted using the Student t-test succeded by Mann-Whitney U investigation (p < .05), while SEM images were assessed qualitatively. RESULTS: The analysis included 45 sound teeth collected from men and women 18 to 50 years old. Post irradiation micromechanical variations of human teeth were significant only in the radiation groups of 30 Gy and 60 Gy compared to pre-irradiation group for enamel (7.24 ± 0.18 MRayl and 6.49 ± 028 MRayl; p < 0.05, respectively). Besides, the teeth subjected to radiation doses of 20, 30 and 60 Gy represented significantly lower acoustic impedance values relative to non-irradiated group for dentin (6.52 ± 0.43 MRayl, 5.71 ± 0.66 MRayl and 4.82 ± 0.53 MRayl p < 0.05), respectively. CONCLUSIONS: These results are evidence for a safe acoustic examination device which may be a useful tool to visualize and follow the safe dose limits to impede severe dental damage through the radiation therapy treatment for head and neck cancers.

Evaluation of Significant Radiographic Findings and Their Impact on the Oral Health-Related Quality of Life of Patients with Complete Dentures.

PURPOSE: The aim of this study was to assess the frequency and location of positive radiographic findings in edentulous patients and to evaluate the oral health-related quality of life (OHRQoL) and complacency of patients with positive radiologic findings. MATERIALS AND METHODS: A total of 1,349 asymptomatic edentulous patients were retrospectively evaluated using a digital panoramic system for the presence of retained root fragments, impacted teeth, foreign bodies, radiolucencies, radiopacities, mental foramen at or near the alveolar crest, and maxillary sinus pneumatization. The patients with positive findings were called for a follow-up to take a new panoramic radiograph. The functional status and symptoms of participants were measured with the Oral Health Impact Profile (OHIP-14). RESULTS: At least one or more of the radiographic findings were observed in 35.21% of the evaluated radiographs. The most frequent finding was sinus pneumatization (20.9%). The mean total OHIP score was 9.74 ± 8.34. There was no statistically significant difference observed for gender, age, education levels, marital status, or having surgical interventions for total OHIP-14 scores (P > .05). CONCLUSION: The results of this study suggest that routine radiographic examination of edentulous patients may not be crucial before rehabilitation, and using a total percentage of positive radiographic findings may result in the exaggeration of the disease risk in edentulous patients.

Does giving brief information keep patients calm during different oral surgical procedures?

OBJECTIVES: Dental anxiety may play a central role in the oral health status and treatment outcomes of oral surgical procedures. The study aimed to investigate the effect that brief written information has over patients undergoing oral surgical procedures and to evaluate factors that may cause anxiety. METHOD AND MATERIALS: A prospective study was performed on 38 mandibular third molar surgery patients (mean age 26.74 ± 6.44 years) and 56 implant surgery patients (mean age 49.13 ± 15.11 years). Each group was divided into two subgroups, and written information, explaining what they could expect and details about the procedure, was provided to study groups. The Spielberger State-Trait Anxiety Inventory was used to measure state (STAI-S) and trait anxiety (STAI-T). The visual analog scale (VAS) was used for pain scores preoperatively and on days 1, 3, 5, and 7. Demographic data and intraoperative behaviors of patients were recorded. RESULTS: All groups had similar anxiety scores at baseline. Preoperative STAI-S and VAS scores were similar between study and control groups (P > .05). Study groups showed significantly lower mean intraoperative anxiety levels (P < .05). The implant group had a significantly lower VAS score (P < .05). STAI-T and preoperative STAI-S were not related to VAS. Postoperative STAI-S and VAS and recuperation were correlated (P < .05). Women showed significantly higher anxiety and VAS scores. CONCLUSION: The patients who received written information did not report lower anxiety scores. However, improved patient cooperation could be achieved with this method. Different surgical procedures may cause anxiety for different reasons.

A survey of local anesthesia education in Turkish dental schools.

The aim of this study was to evaluate differences with regard to local anesthesia education in Turkish dental schools. Questionnaires designed to collect information about local anesthesia education were sent to the heads of the Departments of Oral and Maxillofacial Surgery of seventeen Turkish dental schools. Eleven returned the completed survey for a response rate of 64 percent. It was determined that dental schools begin the theoretical part of their local anesthesia (LA) curricula during the first half of the third year. Most dental schools start teaching the practical aspects during the second half of the third year on average--half a year after the beginning of the theoretical background. The first injection in humans, usually a fellow student (82 percent), is mostly supervised by an oral and maxillofacial surgeon. The number of injections under supervision usually depends upon the individual capabilities of the student. None of the schools said they required permission of a medical ethics committee for injections on fellow students. Seventy-three percent of the schools said they were satisfied with their current LA teaching and were not planning to make any changes. Overall, LA teaching programs showed minor variations across the surveyed Turkish dental schools.

Evaluation of reasons for extraction of crowned teeth: a prospective study at a university clinic.

OBJECTIVE: The aim of this study was to determine and classify the major reasons for extraction of teeth with full crowns belonging to patients admitted to a university clinic within a specific time frame. METHODS: Two hundred and eight extracted teeth with full crown belonging to 75 patients were included. Apart from demographic information, reasons for presenting to the hospital, the major reason for extraction, the type of the crown (single crown, bridge abutment, abutment for removable prosthesis), presence of posts, age of restorations, presence of an endodontic treatment and quality were recorded. Comparable data were statistically analyzed using the chi-square test. RESULTS: Forty-three (57.3%) of the patients were females, whereas 32 (42.7%) were males with an average age of 63.21 ± 13.56. A statistically significant relationship was determined between age groups and rate of extractions, with the lowest extractions detected in the 35-44 years age group and the highest in the 55-64 years age group (p = 0.001). Tooth mobility was the predominant reason for patients' initial presentation (52.9%). The most encountered reason for extraction was periodontal reasons with a prevalence of 59.1%, followed by caries (26.9%) and periapical lesions (12%); 35.6% of extracted teeth had endodontic treatment. Sixty teeth (81.1%) had incomplete root fillings. There was a significant relationship between extractions and incomplete root fillings (p = 0.001). CONCLUSION: Studies comprising other faculty clinics as well as general practices will be complementary in making more generalized statements regarding the etiology of extractions of crowned teeth.