RESUMO
The plasma seed treatment is effective for promoting seed germination in many crops. However, the biological mechanism remains unclear. Therefore, mRNA sequencing was used to screen differentially expressed genes in the germination process of Andrographis paniculata seeds treated with air plasma (power density = 8.99 J/cm3). Following plasma treatment, the germination percentages were significantly higher than those of the control, they were 3.5±0.6% vs. 0 at 28 hours after sowing (HAS) and 50.3±2.6% vs. 37.3±1.7% at 48 HAS. After unigenes were assembled and annotated, 125 differentially expressed genes (DEGs) were detected at 28 HAS, compared with nine DEGs at 48 HAS, but no DEGs were detected at 0 HAS, indicating that air plasma treatment mainly changed the gene expression of A. paniculata seeds at 28 HAS. The NCED5 expression level of the treated group was less than one-fifth of the control, and the expressions of three ethylene response factors were significantly higher than the control at 28 HAS, indicating that lower abscisic acid levels play an important role and ethylene signal transduction also participates in radicle protrusion. ACO, NRT1 and PRP3 expressions were significantly higher than in the control at 48 HAS, suggesting that higher ethylene levels cause the endosperm cap to weaken and start to grow root hairs and lateral roots earlier. These findings reveal that plasma promotes seed germination mainly by regulating the expression of hormone-related genes. And the possible signal transduction of related hormones was discussed.
Assuntos
Andrographis/genética , Germinação/genética , Sementes/genética , Transcriptoma/genética , Ácido Abscísico/metabolismo , Ar , Andrographis/metabolismo , Endosperma/genética , Etilenos/metabolismo , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas/genética , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Sementes/metabolismoRESUMO
Diabetes mellitus (DM) is a serious metabolic disorder which has reached epidemic proportions all over the world. Despite tremendous developments in medicinal chemistry, traditional medicine is still commonly used for the prevention and treatment of DM. Traditional herbal medicines have played a major role in the management of DM in Asian countries. In particular, mulberry has commonly been utilized in China for the treatment of DM for thousands of years. In the last decade, numerous preclinical findings have suggested that mulberry is a promising therapeutic agent for the treatment of DM, and the polyhydroxylated alkaloids, flavonoids and polysaccharides from mulberry may be the potential active components. The present review systematically summarizes the chemical composition of mulberry and the pharmacological effects of different medicinal parts on DM; these effects include influences on glucose absorption, insulin (INS) production/secretion, anti-oxidation and anti-inflammation processes. After summarizing our research findings, we will discuss the challenges and opportunities and explore the direction of future research and the potential for developing mulberry into pharmaceuticals for the widespread treatment of DM.
Assuntos
Diabetes Mellitus/tratamento farmacológico , Hipoglicemiantes , Morus/química , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Alcaloides/química , Alcaloides/isolamento & purificação , Alcaloides/farmacologia , Animais , Anti-Inflamatórios , Antioxidantes , Diabetes Mellitus/prevenção & controle , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Glucose/metabolismo , Humanos , Hidroxilação , Secreção de Insulina/efeitos dos fármacos , Medicina Tradicional Chinesa , Morus/anatomia & histologia , Extratos Vegetais/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologiaRESUMO
Based on a systematic review of morphology and distribution of plants, alternate names, actions, and properties of herbs recorded in ancient and modern literatures, in combination of field investigation, 18 Chinese herbal medicines recorded in ancient bencao literature were regarded to be derived from 7 species in the Ardisia genus. Among them, the variety Ardisia crenata f. hortensis was identified as the source of Zhushagen and Zijinniu. A. hanceana is referenced as Tiesan in the illustrated atlas of Botanical Nomenclature (Zhiwu Mingshi Tukao). The name Pingdimu refers to a different substance in the illustrated atlas of Botanical Nomenclature and the Flower Mirror (Huajing). The medicinals named Yedihong, Aicha, and Duanjiao sanlangare all derived from A. japonica. The origin of the herb Xiaoqing referenced in the Illustrated Classic of the Materia Medica (Bencao Tujing) is A. pusilla. The medicinals Bailiangjin, Jiuguanxue and Zoumatai are derived from A. crispa, A. brevicaulis, and A. gigantifolia, respectively. This investigation clarifies the botanical sources and actions of related Chinese medicinal materials in the genus Ardisia, and provides clues and evidence for utilizing and developing their medicinal plant resources.