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[This retracts the article DOI: 10.3892/ol.2017.6777.].
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MicroRNAs (miRs) are involved in many aspects of cell biology, including cell proliferation and apoptosis, two critical aspects of tumor biology. We investigated the effect of miR-711 on Bcl-2 expression in human MGC803 gastric cancer cells and the mechanism of cell proliferation, apoptosis, and invasion. Expression of miR-711 and Bcl-2 was significantly increased in gastric adenocarcinoma compared to adjacent normal tissue. Inhibition of miR-711 in MGC803 gastric cancer cells decreased the expression of Bcl-2, decreased cell proliferation, decreased the invasion ability, and increased apoptosis. The expression of Bcl-2 protein correlated with clinical staging, lymph node metastasis, and tumor differentiation in patients with gastric cancer. The expression of miR-711 positively correlated with the expression of Bcl-2, suggesting that miR-711 and Bcl-2 are co-regulated and involved in the development of gastric cancer.
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OBJECTIVES: To investigate the protective effects and mechanism of antioxidant TBHQ on renal damage caused by doxorubicin chemotherapy in mice with hepatic cancer. METHODS: Cell H22 of mice with hepatic cancer which was subcultured for three times was subcutaneously transplanted to the groin of right lower limb of 45 SPF Kunming mice to establish the transplanted tumor model. The doxorubicin chemotherapy group and antioxidant intervention group received intraperitoneal injection of ADM (1 mg/kg·0.2 mL/2 d). The model control group received normal saline (NS) of the same volume at the same time. 1% TBHQ was added into the diet of mice of the antioxidant intervention group. Seven weeks later, morning urines and peripheral blood were randomly collected to detect UAlb, UCr, BUN, Scr and UAlb/Cr levels. All mice were beheaded. The renal tissues were made into homogenate, and SOD, T-AOC and MDA content in tissues were detected followed by cell lysis. All data were processed using SPSS19.0. RESULTS: The UAlb/Cr, BUN, Scr and MDA of doxorubicin chemotherapy group were significantly higher those of model control group and the activities of SOD, T-AOC in doxorubicin chemotherapy group were lower than those of model control group (P < 0.01). The UAlb/Cr, BUN, Scr and MDA of antioxidant intervention group were lower than those of doxorubicin chemotherapy group and the activities of SOD, T-AOC of antioxidant intervention group were higher than those of doxorubicin chemotherapy group doxorubicin chemotherapy group (P < 0.05). The BUN of model control group was higher than that of blank group, and T-AOC was lower than that of blank group, and difference was statistically significant (P < 0.05). CONCLUSIONS: Doxorubicin chemotherapy could lead to abnormal antioxidant capacity and renal function of tumor-bearing mice with hepatic cancer. TBHQ antioxidant intervention could effectively improve the antioxidant capacity of renal tissue and reduce the renal damage caused by doxorubicin to some extent.