RESUMO
Ascochyta blight is the major disease attacking chickpea (Cicer arietinum) around the world. Since its first time report of isolation in Argentina in 2012, the pathogen has caused severe economic losses and has acquired a great importance. We report here the isolation of Ascochyta rabiei from infected chickpea beans cultivated in Santa Fe, Argentina; its identification by morphological analysis and molecular biology techniques based on internal transcribed spacer (ITS) sequence alignment, its biochemical characterization regarding the capacity to produce proteinase and phospholipase enzymes, and its antifungal susceptibility to common used antifungal agents. In order to detect new inhibitors for A. rabiei from natural sources, a bioautographic method was developed. From the screening method developed, we found that extracts from cultures of Aspergillus parasiticus are active against A. rabiei.
Assuntos
Ascomicetos/classificação , Ascomicetos/isolamento & purificação , Cicer/microbiologia , Doenças das Plantas/microbiologia , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Argentina , Ascomicetos/citologia , Ascomicetos/fisiologia , Aspergillus/química , Produtos Biológicos/isolamento & purificação , Produtos Biológicos/farmacologia , DNA Fúngico/química , DNA Fúngico/genética , DNA de Plantas/química , DNA de Plantas/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Testes de Sensibilidade Microbiana , Microscopia , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Análise de Sequência de DNARESUMO
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.