Case report: plasma cell gingivitis A.

The plasma cell gingivitis condition, an uncommon, nonmalignant lesion, is characterized by an offending allergen which causes a massive plasma cell infiltration in gingival connective tissue. Occasionally, soft tissue biopsy of affected and normal tissues is necessary for proper diagnosis. Blood analysis may rule out other systematic disorders with oral manifestations. This case report describes the condition.

Metastatic breast carcinoma mandible in gynecomastia gynecomastic. Case report.

This is a rare case of breast carcinoma with metastasis to the jaw in a relatively young man with gynecomastia. Immunohistochemical studies suggest a stage differentiation arrest in ductal cells.

Large cell, multilobated, B-cell lymphoma of the palate. A case report.

A case of B-large cell non-Hodgkin's lymphoma (NHL) with multilobated nuclei arising in the palatal mucosa is described. Immunologic typing of tumor cells was crucial to determine the exact cell of origin of this lesion.

Coexpression of cytokeratins, involucrin, and blood group antigens in oral squamous cell carcinomas.

The well and poorly differentiated oral squamous carcinomas preferentially express proteins, blood group antigens, and contain associated dendritic Langerhans' cells. Keratin pearls in well-differentiated carcinomas simulate the differentiation pathway of the normal oral squamous epithelium, whereas poorly differentiated carcinomas do not and appear more heterogeneous. Terminally keratinized cells correlate with involucrin and expression of blood group antigens in keratin pearls, a feature that differs from the nonkeratinizing normal epithelium in which such carcinomas arise. Dendritic Langerhans' cells are reduced in number in squamous carcinomas.

Immunohistochemical localization of Leu-M1 carbohydrate antigen in human oral squamous cell carcinoma.

Twenty-six biopsy specimens of oral squamous cell carcinomas were examined by the avidin biotin peroxidase complex (ABC) method for the presence of an epithelial cell membrane bound lacto-N-fucopentaose III, known also as Leu-M1 or Lex antigen. In normal oral epithelium, Leu-M1 antigen was expressed on keratinizing epithelia in the stratum spinosum. In well-differentiated carcinomas the antigen was found on the cell membrane of nucleate cells in infiltrating epithelial islands. Such pattern in moderately well and in poorly differentiated carcinomas was minimally expressed and was associated with flattened squamous cells or otherwise recorded negative. Leu-M1 antigen immunoreactivity in normal oral epithelia and in carcinomas was comparable to that of blood group H-2 chain that were examined. It was concluded that the intensity of the reaction parallels the magnitude of differentiation of epithelia. Leu-M1 antigen can serve as a marker of differentiation in oral squamous epithelium.

Crohn's disease: early detection by gingival biopsy.

A definitive diagnosis of Crohn's disease was made in a 31-year-old female patient who presented with a chief complaint of red, burning gingiva and an itchy palate. The microscopic identification of multinucleated giant cells in the gingival biopsy was suggestive of Crohn's disease. This led to a complete medical work-up including an upper and lower bowel series, which confirmed the diagnosis of Crohn's disease.

T-cell lymphoma of palatal midline. Report of 2 cases.

2 cases of unifocal T-cell lymphomas, predominantly large cell type, one restricted to the midline of the palate and the other involving the nasal cavity are reported. Monoclonal antibodies served as a useful adjunct to light microscopy in diagnosing these tumors.

Expression of endothelial cell markers PAL-E and EN-4 and Ia-antigens in Kaposi's sarcoma.

Eleven biopsy specimens of Kaposi's sarcoma (KS) removed from the skin and oral mucosa were examined immunohistochemically with monoclonal antibodies PAL-E and EN-4, specific for human vascular endothelial cells, and with LN-3 monoclonal antibody reactive with immune-associated (Ia) antigens in the HLA-DR locus. The early lesions of KS, corresponding to the patch phase, contained hyperplastic venules and an increased number of lymphatic capillaries. The lymphatic capillary endothelium was reactive with EN-4, whereas, PAL-E reacted only with blood vessel endothelial cells. The spindle cells, like lymphatic endothelial cells, were non-reactive with PAL-E but showed positive reaction with EN-4 antibodies. The observed morphologic pattern of vasculogenesis and the demonstrated immune-reactivity in KS support an origin from the venule-lymphatic junction. This is an aberrant pattern but reminiscent of normal embryonal lymphatic channel development. The lymphatic capillaries and vascular slits were nonreactive with LN-3 antibody, but it was positive on cell membranes in a number of spindle cells, suggesting the focal expression of Ia-antigens.

Histochemical study of lectin binding to gestational endometrium.

Avidin-biotin-peroxidase labeling technique was used to localize the binding sites of concanavalin A, peanut agglutinin, and Ulex europaeus in 30 formalin-fixed paraffin-embedded samples of gestational endometrium in intrauterine and ectopic pregnancy, in comparison with 23 samples of normal endometrium at different stages of the menstrual cycle. Concanavalin A shows strong affinity to decidual cells; in normal endometrium its affinity to stroma is weak to moderate. Ulex europaeus, which is weakly positive in normal endometrium, demonstrates focal strong reaction with epithelium and intraluminal secretions in some of the gestational glands. Peanut agglutinin is the most remarkable marker of pregnancy-related changes in endometrium, being strongly positive in the apical portions and intraluminal secretions of the glands; in nonpregnant endometrium its binding capacity is weak.

An immunohistochemical study of oral lichen planus.

Specimens from 22 cases of oral lichen planus were prepared for studies of the basement membrane zone by immunohistochemical and histochemical methods. Langerhans' cells, monocytes, and lymphocytes constitute the predominant cells of the inflammatory infiltrate, thus indicating a cell-mediated response. Lymphocyte-related necrosis of epithelium and nonspecific vasculitis are associated with an altered basement membrane zone. Immunoproteins, including IgG, IgA, IgM, and C'3 as well as fibrinogen, are present and distributed in a nonspecific pattern.

Product identification of ameloblastomas: an immunohistochemical study.

Twenty-nine solid, plexiform and follicular ameloblastomas and control human developing teeth at different stages of maturation were formalin-fixed, paraffin-embedded and sectioned for the study of laminin, fibronectin, and mannosyl, glucosyl, and galactosyl residues using the peroxidase antiperoxidase (PAP) and avidin-biotin peroxidase complex (ABC) staining methods, respectively. Monospecific antibodies to laminin and fibronectin and the affinity of the lectins for mannosyl, glucosyl, and galactosyl residues with RCA-I. Laminin is present during the stage of morphodifferentiation of normal tooth development within the basement membrane. Following cytodifferentiation and calcified matrix deposition, laminin is not detectable. Laminin is also found in the basement membrane of both follicular and plexiform ameloblastomas. In contrast, fibronectin is reactive only up to the stage of morphodifferentiation among the mesenchymal cells and at the epithelial-mesenchymal interface, and it becomes unreactive upon differentiation of odontoblasts and mesenchymal pulp tissue as well. Unlike laminin, fibronectin is unreactive in the basement membrane of plexiform follicular and ameloblastomas.

Product definition in a case of myoepithelioma.

One case of myoepithelioma was studied by immunohistochemical and histochemical methods to identify the cell products of this tumor. The myoepithelioma had two cell types: plasmacytoid cells and spindle cells. The tumor myoepithelial cells produced the same cell products as did the normal myoepithelial cells but in variable amounts, possibly related to stages of differentiation. As no exocrine cell products could be identified in this neoplasm, it is considered a "true" myoepithelioma.

Hamster cheek pouch carcinoma: effect of incision and cortisone on growth, invasion, and metastasis.

After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies performed on tumors in their pouches, one of these also received injections of cortisone throughout the 20-week experimental period, and a fourth group received cortisone only. The wet weights of the cancerous cheek pouches were determined, and the submandibular and parotid salivary glands with associated cervical lymph nodes, the lungs, and the liver were examined with light microscopy. The cancerous pouches of the animals that received cortisone weighed significantly less than those of animals that received no cortisone but had incisional biopsies of the tumors. There was no significant difference in the degree of histodifferentiation of the tumors among the four groups. The animals in the two groups that received cortisone had significantly more tumors that were invasive than did the animals that did not receive cortisone. Cervical lymph node metastasis occurred in 21% to 38% of the animals but was not significantly different in the four groups. Distant metastases to the lungs or the liver were not found. Incisional biopsy of the tumors stimulated local growth of the cheek pouch tumors, and systemic cortisone administration produced more invasive cheek pouch tumors.

Lectin binding to normal, dysplastic, and neoplastic cervical epithelium.

Avidin-biotin-peroxidase labeling technic was used to localize the binding sites of Concanavalin agglutinin (Con A), Ricinus communis (RCA-I), Ulex europaeus (UEA-I), and Limus flafus (LFA) in the cervical epithelia afflicted with condyloma (2 cases), moderate dysplasia (6), severe dysplasia (3), carcinoma in situ (9), squamous cell carcinoma (18), adenosquamous carcinoma (2), adenocarcinoma (1), and glassy cell carcinoma (1). Normal squamous epithelium displayed binding sites predominantly located on the cellular membranes for all the tested lectins except UEA. Normal glandular epithelium showed cytoplasmic localization of the lectins. Neoplastic transformation of squamous epithelium was associated with an increased intensity of the reaction and the appearance of the binding sites in the cytoplasm. UEA binding has changed from negative in normal epithelium to moderately positive in dysplasia and strongly positive in carcinoma. Invasive squamous carcinomas demonstrated an extremely variable pattern of lectin binding, some with very high intensity, allowing easy recognition of malignant cells even in minute metastatic foci.

Wheat germ and peanut agglutinin binding to normal, dysplastic and neoplastic cervical epithelium.

Binding of wheat germ agglutinin (WGA) and peanut agglutinin (PNA) was studied cytochemically in 43 cervical specimens covering a range of lesions from moderate atypia to invasive carcinoma. Normal squamous epithelium showed weak binding of WGA and negative reaction with PNA. Atypical squamous epithelium was characterized by moderate binding of WGA and PNA, while malignant lesions demonstrated strong affinity to both lectins. In adenocarcinoma in situ, WGA and PNA binding was reduced by comparison with normal glandular epithelium, that was strongly positive.

Lectin binding to normal human endometrium.

Avidin-biotin-peroxidase labelling technique was used to localize the binding sites of peanut agglutinin (PNA), Ulex europaeus (UEA-I), wheat germ agglutinin (WGA), concanavalin A (Con A), and Ricinus communis (RCA-I) in 30 samples of endometrium at different stages of the menstrual cycle and in early pregnancy. PNA and UEA-I exhibit weak binding to the apical portions of epithelial cells in proliferative and secretory phases, but become strongly positive in pregnancy. WGA and RCA-I show weak binding to glandular epithelium in the proliferative phase, but increase their affinity in the secretory phase. Con A demonstrates strong affinity to decidual cells.