RESUMO
Sample preservation often impedes efforts to generate high-quality reference genomes or pangenomes for Earth's more than 2 million plant and animal species due to nucleotide degradation. Here we compare the impacts of storage methods including solution type, temperature, and time on DNA quality and Oxford Nanopore long-read sequencing quality in 9 fish and 4 plant species. We show 95% ethanol largely protects against degradation for fish blood (22 °C, ≤6 weeks) and plant tissue (4 °C, ≤3 weeks). From this furthest storage timepoint, we assemble high-quality reference genomes of 3 fish and 2 plant species with contiguity (contig N50) and completeness (BUSCO) that achieve the Vertebrate Genome Project benchmarking standards. For epigenetic applications, we also report methylation frequency compared to liquid nitrogen control. The results presented here remove the necessity for cryogenic storage in many long read applications and provide a framework for future studies focused on sampling in remote locations, which may represent a large portion of the future sequencing of novel organisms.
Assuntos
Genoma , Genômica , Animais , Genômica/métodos , Análise de Sequência de DNA/métodos , Peixes/genéticaRESUMO
Possession of the neck allows vertebrates to move the head independently from the trunk. Fish do not have the neck and thus the cranial mobility could be limited. Oxudercine gobies show full range of habitat transition from aquatic to terrestrial environments and exhibit flexible cranial movement, yet the cranium-movement apparatus is little known. In this study, we investigated the anatomy of the structure of the eight oxudercine gobies, Oxuderces nexipinnis, Parapocryptes serperaster, Pseudapocryptes elongatus, Scartelaos histophorus, Boleophthalmus boddarti, Periophthalmus chrysospilos, Periophthalmodon schlosseri, and Periophthalmodon septemradiatus. These species share similarities in the specialized features of the craniovertebral joint and the epaxials attaching onto different locations of the neurocranium. On the other hand, large space between the ventral portions of the craniovertebral joint only occurs in O. nexipinnis, Pd. elongatus, Pn. schlosseri and Pn. septemradiatus. Hypaxials are hypertrophied at the insertion point and attach more anteriorly onto the ventral side of the neurocranium in B. boddarti, O. nexipinnis, Pa. serperaster, Pd. elongatus, and S. histophorus, whereas the muscles are small and attach posteriorly in the remaining species. There were significant differences in the area occupancy ratio of the post-cranial neural spines, the lever arm ratio of the cranial rotation, and the angle between the horizontal plane and the plane through the craniovertebral joint among the species. The cranial depression presumably facilitates grazing of oxudercine gobies in their early stage of terrestrial transition, whereas the cranial elevation parameters are contradictory to the terrestrial gradient. The cranium-movement morphometrics partially agree with the phylogeny.
Assuntos
Perciformes , Animais , Ecossistema , Movimento , Perciformes/fisiologia , Filogenia , CrânioRESUMO
There has been a long-standing controversy about whether vertebrates emerged in the Paleozoic from marine or freshwater environments. Several hypotheses have proposed coastal, estuarine and riparian areas as sites of the transition. Here, we report the ecology of an amphibious fish Periophthalmodon septemradiatus, which we presume is in the process of niche expansion into terrestrial habitats from estuarine to freshwater environments along the Mekong River, Vietnam. Adult fish are highly terrestrial and have not been observed to venture into water during our survey. Courtship behaviour was observed, and fertilised eggs were recovered from burrows in both brackish and freshwater environments. The smallest fish collected at 12, 96, and 148 km from the river mouth were juveniles shortly after starting an amphibious life. These findings suggest reproduction in both brackish and freshwater environments. In contrast, otolith Sr:Ca ratio indicates larval hatching only in brackish water. Analysis of a 940-base pair (bp) segment of the mitochondrial cytochrome c oxidase subunit II and a 934-bp segment of the mitochondrial D-loop demonstrated no genetic segregation between populations. The fish may provide a unique opportunity to study how ambient salinity affects the biology and ecology of a living vertebrate during transition from water to land.
Assuntos
Ecossistema , Peixes/fisiologia , Água Doce , Águas Salinas , Adaptação Fisiológica , Distribuição Animal , Animais , Comportamento Animal , Tamanho Corporal , Corte , DNA Mitocondrial/genética , Ovos , Complexo IV da Cadeia de Transporte de Elétrons/genética , Estuários , Feminino , Peixes/genética , Peixes/crescimento & desenvolvimento , Masculino , Rios , Salinidade , VietnãRESUMO
OBJECTIVES: This study aimed to examine the relationships among self-reported oral health, oral hygiene practices, and oral human papillomavirus (HPV) infection in women at risk for sexually transmitted infections (STIs) in Ho Chi Minh City, Vietnam. STUDY DESIGN: Convenience and referral sampling methods were used in a clinic-based setting to recruit 126 women aged 18-45 years between August and October 2013. Behavioral factors were self-reported. Oral-rinse samples were tested for HPV DNA of 2 low-risk and 13 high-risk genotypes. RESULTS: A higher unadjusted prevalence of oral HPV infection was associated with poorer self-rated overall oral health (P = .001), reported oral lesions or problems in the past year (P = .001), and reported a tooth loss not because of injury (P = .001). Higher unadjusted prevalence of oral HPV infection was also associated with two measures of oral hygiene: lower frequencies of toothbrushing per day (P = .047) and gargling without toothbrushing (P = .037). After adjusting for other factors in multivariable logistic regression models, poorer self-rated overall oral health remained statistically associated with oral HPV infection (P = .042); yet the frequency of tooth-brushing per day did not (P = .704). CONCLUSION: Results corroborate the association between self-reported poor oral health and oral HPV infection. The effect of oral hygiene on oral HPV infection remains inconclusive.
Assuntos
Doenças da Boca/epidemiologia , Doenças da Boca/virologia , Saúde Bucal , Higiene Bucal , Infecções por Papillomavirus/epidemiologia , Autorrelato , Adolescente , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Fatores de Risco , Vietnã/epidemiologiaRESUMO
UNLABELLED: Background Evidence regarding whether male partners' characteristics can influence women's likelihood of getting sexually transmissible infections (STIs) is insufficient and inconsistent. Our study examined associations between women's perception of primary male partners' demographic and behavioural characteristics and women's history of bacterial STI diagnoses among 126 women at risk for STIs in Ho Chi Minh City, Vietnam. METHODS: All variables were obtained by women's self-report. Due to the excess zeroes of the number of bacterial STI diagnoses for women, we used Zero-Inflated Poisson regression to examine associations of interest. RESULTS: Among women who had one lifetime male partner (n=49), the partner's number of female sexual partners [prevalence ratio (PR)=4.63, 95%CI=1.44-14.88] and number of STI diagnoses (PR=27.32, 95%CI=1.56-477.70) were associated with the woman's number of bacterial STI diagnoses, after adjusting for women's education level. For women who had >1 lifetime male partner (n=77), a greater number of women's bacterial STI diagnoses was also associated with their partners' STI history (PR=9.12, 95% CI=2.44-34.11). CONCLUSIONS: An increased risk for STIs in women was associated with both individual characteristics and their primary male partner's behavioural risk factors. Therefore, primary male partners' risk factors should be included in STI risk assessments, treatment and interventions for women.
RESUMO
BACKGROUND: Concurrent infection with multiple types of Human Papillomavirus (HPV) is associated with an increased risk of cervical cancer; yet, little is known about risk factors for concurrent HPV infection in Vietnam. This study investigated the prevalence of and risk factors for high-risk-type HPV and multi-type HPV infections among women in Ho Chi Minh City, Vietnam. METHODS: Data were collected from a population-based survey of 1,550 women (mean age = 42.4; SD = 9.5), using a multi-stage sampling process. Socio-demographic and behavioral variables were obtained by self-report. HPV genotypes in cervical specimens were identified using PCR protocols. RESULTS: The prevalence of any high-risk HPV infection was 9.0%, and of multi-type HPV infection was 1.9%. In the HPV+ subsample, the percentage of high-risk HPV was 84% and of multi-type HPV was 20%. All multi-type HPV infections were high-risk-type. Lifetime smoking and older age of first sex were significantly associated with any high-risk and multi-type HPV infections. Regular condom use was inversely associated with high-risk and multi-type HPV infection. CONCLUSIONS: Risk factors for high-risk and multi-type HPV infections were similar. Further research and intervention are needed to reduce HPV infections in order to prevent HPV-related cancers.
Assuntos
Coinfecção/epidemiologia , Preservativos/estatística & dados numéricos , DNA Viral/análise , Papillomaviridae/genética , Infecções por Papillomavirus/epidemiologia , Comportamento Sexual/estatística & dados numéricos , Fumar/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Coinfecção/virologia , Estudos Transversais , Feminino , Genótipo , Humanos , Pessoa de Meia-Idade , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Fatores de Risco , Vietnã/epidemiologia , Adulto JovemRESUMO
Traumatic brain injury continues to be a major socioeconomic problem, costing the United States $76.5 billion in the year of 2000. Despite the advances in the field of medicine, there are still no definitive treatments for traumatic brain injury. Goal of therapy is still gearing toward supportive cares such as intracranial pressure monitoring, lowering intracranial pressure, correcting cerebral ischemia, and manipulating serum osmolarity. The search for effective treatment in human studies has been unfruitful. In this review, the mechanisms of primary and secondary brain injury are discussed along with potential neuroprotective interventions such as hyperosmolar therapies, hypothermia, statins, and cyclosporin A.
Assuntos
Lesões Encefálicas/fisiopatologia , Lesões Encefálicas/terapia , Mortalidade Hospitalar/tendências , Hipotermia Induzida/métodos , Fármacos Neuroprotetores/farmacologia , Lesões Encefálicas/mortalidade , Causas de Morte , Terapia Combinada , Compreensão , Ciclosporinas/uso terapêutico , Feminino , Humanos , Escala de Gravidade do Ferimento , Masculino , Avaliação das Necessidades , Fármacos Neuroprotetores/uso terapêutico , Prognóstico , Medição de Risco , Resultado do TratamentoRESUMO
The aim of this study is to determine the contribution of the ciliary epithelium to glutathione (GSH) levels in the aqueous by mapping GSH metabolism and transport pathways in the rat ciliary body. Using a combination of molecular and immunohistochemical techniques, we screened and localised enzymes and transporters involved in GSH synthesis, uptake, efflux and degradation. Our findings indicate that both the pigmented epithelial (PE) and the non-pigmented epithelial (NPE) cell layers are capable of accumulating precursor amino acids for GSH synthesis, but only the NPE cells appear to be involved in the direct uptake of precursor amino acids from the stroma. The localisation of GSH efflux transporters to the PE cell and PE-NPE interface indicates that GSH and potentially GSH-S conjugates can be removed from the ciliary epithelium into the stroma, while the location of GSH efflux transporters to the basolateral membrane of the NPE indicates that these cells can mediate GSH secretion into the aqueous. GSH secreted by the ciliary into the aqueous would remain largely intact due to the absence of the GSH degradation enzymes γ-glutamyltranspeptidase (γ-GGT) labelling at the basolateral membrane of the NPE. Therefore, it appears that the ciliary epithelium contains the molecular machinery to mediate GSH secretion into the aqueous.