Extraction, Modification, Biofunctionality, and Food Applications of Chickpea (Cicer arietinum) Protein: An Up-to-Date Review.

Plant-based proteins have gained popularity in the food industry as a good protein source. Among these, chickpea protein has gained significant attention in recent times due to its high yields, high nutritional content, and health benefits. With an abundance of essential amino acids, particularly lysine, and a highly digestible indispensable amino acid score of 76 (DIAAS), chickpea protein is considered a substitute for animal proteins. However, the application of chickpea protein in food products is limited due to its poor functional properties, such as solubility, water-holding capacity, and emulsifying and gelling properties. To overcome these limitations, various modification methods, including physical, biological, chemical, and a combination of these, have been applied to enhance the functional properties of chickpea protein and expand its applications in healthy food products. Therefore, this review aims to comprehensively examine recent advances in Cicer arietinum (chickpea) protein extraction techniques, characterizing its properties, exploring post-modification strategies, and assessing its diverse applications in the food industry. Moreover, we reviewed the nutritional benefits and sustainability implications, along with addressing regulatory considerations. This review intends to provide insights into maximizing the potential of Cicer arietinum protein in diverse applications while ensuring sustainability and compliance with regulations.

Recent Trends in Cereal- and Legume-Based Protein-Mineral Complexes: Formulation Methods, Toxicity, and Food Applications.

Minerals play an important role in maintaining human health as the deficiency of these minerals can lead to serious health issues. To address these deficiencies, current research efforts are actively investigating the utilization of protein-mineral complexes as eco-friendly, non-hazardous, suitable mineral fortifiers, characterized by minimal toxicity, for incorporation into food products. Thus, we reviewed the current challenges in incorporating the cereal-legume protein-inorganic minerals complexes' structure, binding properties, and toxicity during fortification on human health. Moreover, we further reviewed the development of protein-mineral complexes, characterization, and their food applications. The use of inorganic minerals has been associated with several toxic effects, leading to tissue-level toxicity. Cereal- and legume-based protein-mineral complexes effectively reduced the toxicity, improved bone mineral density, and has antioxidant properties. The characterization techniques provided a better understanding of the binding efficiency of cereal- and legume-based protein-mineral complexes. Overall, understanding the mechanism and binding efficiency underlying protein-mineral complex formation provided a novel insight into the design of therapeutic strategies for mineral-related diseases with minimal toxicity.

Exploring Microbial Contributions to Nutraceutical Production: From Natural to Designed Foods.

For ages, societies throughout the world have used fermentation as a traditional method for food processing and preservation, helping to create a wide range of staple foods and delicacies. Due to its possible health advantages, mostly attributable to the inclusion of bioactive substances known as nutraceuticals, fermented foods have attracted a lot of interest recently. This in-depth analysis examines the wide range of nutraceuticals present in fermented foods, as well as how they are made, what health benefits they may have, and how they may be used in the nutraceutical and functional food businesses. By stressing how important fermented foods are as a source of beneficial bioactive components that support human health and well-being. Numerous bioactive substances found in fermented foods have been the subject of recent scientific studies. These molecules may find use in the pharmaceutical and nutraceutical sectors. Streptococcus thermophilus, Lactobacillus gasseri, Lactobacillus delbrueckii, Lactobacillus bulgaricus, and Lactobacillus johnsonii are just a few examples of the probiotic bacteria that live in fermented foods and formulas. This review elucidates the importance of microorganisms sourced from fermented foods as potent agents for diverse nutraceuticals and their potential role in preventing various diseases whilst serving as functional food supplements.

Recent Strategies for the Remediation of Textile Dyes from Wastewater: A Systematic Review.

The presence of dye in wastewater causes substantial threats to the environment, and has negative impacts not only on human health but also on the health of other organisms that are part of the ecosystem. Because of the increase in textile manufacturing, the inhabitants of the area, along with other species, are subjected to the potentially hazardous consequences of wastewater discharge from textile and industrial manufacturing. Different types of dyes emanating from textile wastewater have adverse effects on the aquatic environment. Various methods including physical, chemical, and biological strategies are applied in order to reduce the amount of dye pollution in the environment. The development of economical, ecologically acceptable, and efficient strategies for treating dye-containing wastewater is necessary. It has been shown that microbial communities have significant potential for the remediation of hazardous dyes in an environmentally friendly manner. In order to improve the efficacy of dye remediation, numerous cutting-edge strategies, including those based on nanotechnology, microbial biosorbents, bioreactor technology, microbial fuel cells, and genetic engineering, have been utilized. This article addresses the latest developments in physical, chemical, eco-friendly biological and advanced strategies for the efficient mitigation of dye pollution in the environment, along with the related challenges.

Nano-Biofertilizer Formulations for Agriculture: A Systematic Review on Recent Advances and Prospective Applications.

In the twenty-first century, nanotechnology has emerged as a potentially game-changing innovation. Essential minerals are mostly unavailable in modern cropping systems without the application of synthetic fertilizers, which have a serious negative impact on the ecosystem. This review focuses on the coupling of nanoparticles with biofertilizers to function as nano-biofertilizers (NBFs), which may ensure world food security in the face of the rising population. The inoculation of plants with NBFs improves plant development and resistance to stress. Metallic nanoparticles as well as organic components comprising polysaccharide and chitosan may be encapsulated, utilizing microbe-based green synthesis to make NBFs, which circumvents the limitations of conventional chemical fertilizers. The application of NBFs is just getting started, and shows more promise than other approaches for changing conventional farming into high-tech "smart" farming. This study used bibliographic analysis using Web of Science to find relevant papers on "nano biofertilizers", "plants", and "agriculture". These subjects have received a lot of attention in the literature, as shown by the co-citation patterns of these publications. The novel use of nanotechnology in agriculture is explored in this research work, which makes use of the unique characteristics of nanoscale materials to address urgent concerns including nutrient delivery, crop protection, and sustainable farming methods. This study attempts to fill in some of the gaps in our knowledge by discussing the formulation, fabrication, and characterization of NBFs, as well as elucidating the mechanisms by which NBFs interact with plants and how this benefits the ability of the plant to withstand biotic and abiotic stress brought about by climate change. This review also addresses recent developments and future directions in farming using NBF formulations in the field.

Valorization of dragon fruit waste to value-added bioproducts and formulations: A review.

Owing to the increasing worldwide population explosion, managing waste generated from the food sector has become a cross-cutting issue globally, leading to environmental, economic, and social issues. Circular economy-inspired waste valorization approaches have been increasing steadily, generating new business opportunities developing valuable bioproducts using food waste, especially fruit wastes, that may have several applications in energy-food-pharma sectors. Dragon fruit waste is one such waste resource, which is rich in several value-added chemicals and oils, and can be a renewable resource to produce several value-added compounds of potential applications in different industries. Pretreatment and extraction processes in biorefineries are important strategies for recovering value-added biomolecules. There are different methods of valorization, including green extractions and biological conversion approaches. However, microbe-based conversion is one of the advanced technologies for valorizing dragon fruit waste into bioethanol, bioactive products, pharmaceuticals, and other valued products by reusing or recycling them. This state-of-the-art review briefly overviews the dragon fruit waste management strategies and advanced eco-friendly and cost-effective valorization technologies. Furthermore, various applications of different valuable bioactive components obtained from dragon fruit waste have been critically discussed concerning various industrial sectors. Several industrial sectors, such as food, pharmaceuticals, and biofuels, have been critically reviewed in detail.

Production of fermentable glucose from bioconversion of cellulose using efficient microbial cellulases produced from water hyacinth waste.

The economic production of cellulase enzymes for various industrial applications is one of the major research areas. A number of broad industrial applications, for example, in cellulosic biomass hydrolysis for simple sugars such as glucose and subsequent biofuel production, make these enzyme systems the third most demanding enzymes. Nevertheless, due to their production on commercial substrates, cellulases fall into the category of costly enzymes. Therefore, the goal of the present work is to evaluate the enhancement of cellulase production and its utilization in the enzymatic hydrolysis of biomass using low-cost cellulosic substrate, which is abundant and widely available. In this context, waste biomasses of water hyacinth (WH), including leaves and stems, have been used as feedstock to produce cellulases via solid-state fermentation (SSF) in the current study, which improves its production as well as activity. Furthermore, the impact of process parameters like temperature and pH has been investigated for improved cellulase production. At optimum concentration using 10 g of feedstock, 22 IU/gds of FP, 92 IU/gds of BGL, and 111 IU/gds of EG have been noticed in day 5 of SSF. Herein, 40 °C has been identified as the optimum temperature for cellulase production, whereas 50-55 °C has been recorded as the optimum reaction temperature for cellulase enzyme activity. Additionally, pH 5.5 has been identified as the optimum pH for cellulase enzyme production, whereas this enzyme was thermally stable (55 °C) at pH 5.0 up to 3.5 h. Further, the cellulosic biomass hydrolysis of WH leaves via an optimized crude enzyme has been performed, and this could release 24.34 g/L of glucose in 24 h of the reaction. The current findings may have potential for developing cellulases for mass-scale production using WH-based waste bioresources for numerous biorefinery applications.

Exploring the potential of lactic acid bacteria and its molecular mechanism of action in the development of biosurfactants: Current finding and future outlook.

Biosurfactants generated from lactic acid bacteria (LAB) offer an advantage over standard microbial surfactants due to their antifungal, antibacterial and antiviral capabilities. Many LAB strains have been related to the manufacture of biosurfactant, an essential chemical with uses in the treatment of a number of illnesses. Furthermore, their effectiveness as anti-adhesive agents against a diverse variety of pathogens proves their utility as anti-adhesive coating agents for medical insertional materials, reducing hospital infections without the need of synthetic drugs and chemicals. LAB produces both low and high molecular weight biosurfactants. Biosurfactants from L. pentosus, L. gasseri and L. jensenii have been reported to produce glycolipopeptides that comprise carbohydrates, proteins and lipids in the ratio of 1:3:6 with palmitic, stearic acid, and linoelaidic acid as the major fatty acid component, whereas L. plantarum has been reported to make surlactin due to the presence of non-ribosomal peptide synthetase genes (NRPS) genes. Antimicrobial activity of sophorolipids and rhamnolipids generated from LAB against B. subtilis, P. aeruginosa, S. epidermidis, Propionibacterium acnes and E. coli has been demonstrated. The safety of biosurfactants is being evaluated in compliance with a number of regulatory standards that emphasize the importance of safety in the pharmaceutical industry. This review attempts, for the first time, to provide a comprehensive evaluation of several approaches for the synthesis of biosurfactant-mediated molecular modulation in terms of their biological value. Future biosurfactant directions, as well as regulatory considerations that are crucial for the synthesis of biosurfactants from novel LAB, have also been explored.

Development of Zn biofertilizer microbeads encapsulating Enterobacter ludwigii-PS10 mediated alginate, starch, poultry waste and its efficacy in Solanum lycopersicum growth enhancement.

In the present farming era, rhizobacteria as beneficial biofertilizers can decrease the negative effects of Zinc (Zn) agrochemicals. However, their commercial viability and utility are constrained by their instability under field conditions. Thus, to enhance their stability, microbial formulations are considered, which will not only offer an appropriate microenvironment, and protection but also ensure a high rate of rhizospheric-colonization. The goal of this study was to create a new formulation for the Zn-solubilizing bacteria E. ludwigii-PS10. The studied formulation was prepared using the extrusion technique, wherein a composite solution containing alginate, starch, zinc oxide, and poultry waste was uniformly mixed with the bacterial strain PS10 to develop low-cost, eco-friendly, and slow-release microbeads. The produced microbead was spherical, and characterized by SEM, FTIR, and XRD. Further, the microbeads were analyzed for their survival stability over 3 months of storage at room temperature and 4 °C. The effect of the microbead on the vegetative growth of tomato plants was investigated. Results showed that 94 % of the encapsulated microbial beads (EMB) matrix was able to encapsulate the bacterial strain PS10. The dried EMB demonstrated a moisture content of 2.87 % and was able to preserve E. ludwigii-PS10 survival at room temperature at the rate of 85.6 %. The application of the microbead to the tomato plants significantly increased plant biomass and Zn content. As a result, our findings support the use of this novel EMB prepared using an alginate/poultry waste/starch mixture to increase bacterial cell viability and plant growth.

Biobased natural deep eutectic system as versatile solvents: Structure, interaction and advanced applications.

The increasing emphasis on the development of green replacements to traditional organic solvents and ionic liquids (ILs) can be attributed to the rising concerns over human health and detrimental impacts of conventional solvents towards the environment. A new generation of solvents inspired by nature and extracted from plant bioresources has evolved over the last few years, and are referred to as natural deep eutectic solvents (NADES). NADES are mixtures of natural constituents like sugars, polyalcohols, sugar-based alcohols, amino acids and organic acids. Interest in NADES has exponentially grown over the last eight years, which is evident from an upsurge in the number of research projects undertaken. NADES are highly biocompatible as they can be biosynthesized and metabolized by nearly all living organisms. These solvents pose several noteworthy advantages, such as easy synthesis, tuneable physico-chemical properties, low toxicity, high biodegradability, solute sustainability and stabilization and low melting point. Research on the applicability of NADES in diverse areas is gaining momentum, which includes as - media for chemical and enzymatic reactions; extraction media for essential oils; anti-inflammatory and antimicrobial agent; extraction of bioactive composites; as chromatographic media; preservatives for labile compounds and in drug synthesis. This review gives a complete overview of the properties, biodegradability and toxicity of NADES which we propose can assist in further knowledge generation on their significance in biological systems and usage in green and sustainable chemistry. Information on applications of NADES in biomedical, therapeutic and pharma-biotechnology fields is also highlighted in the current article along with the recent progress and future perspectives in novel applications of NADES.

Microbial biosorbent for remediation of dyes and heavy metals pollution: A green strategy for sustainable environment.

Toxic wastes like heavy metals and dyes are released into the environment as a direct result of industrialization and technological progress. The biosorption of contaminants utilizes a variety of biomaterials. Biosorbents can adsorb toxic pollutants on their surface through various mechanisms like complexation, precipitation, etc. The quantity of sorption sites that are accessible on the surface of the biosorbent affects its effectiveness. Biosorption's low cost, high efficiency, lack of nutrient requirements, and ability to regenerate the biosorbent are its main advantages over other treatment methods. Optimization of environmental conditions like temperature, pH, nutrient availability, and other factors is a prerequisite to achieving optimal biosorbent performance. Recent strategies include nanomaterials, genetic engineering, and biofilm-based remediation for various types of pollutants. The removal of hazardous dyes and heavy metals from wastewater using biosorbents is a strategy that is both efficient and sustainable. This review provides a perspective on the existing literature and brings it up-to-date by including the latest research and findings in the field.

Transformation of Agro-Waste into Value-Added Bioproducts and Bioactive Compounds: Micro/Nano Formulations and Application in the Agri-Food-Pharma Sector.

The agricultural sector generates a significant amount of waste, the majority of which is not productively used and is becoming a danger to both world health and the environment. Because of the promising relevance of agro-residues in the agri-food-pharma sectors, various bioproducts and novel biologically active molecules are produced through valorization techniques. Valorization of agro-wastes involves physical, chemical, and biological, including green, pretreatment methods. Bioactives and bioproducts development from agro-wastes has been widely researched in recent years. Nanocapsules are now used to increase the efficacy of bioactive molecules in food applications. This review addresses various agri-waste valorization methods, value-added bioproducts, the recovery of bioactive compounds, and their uses. Moreover, it also covers the present status of bioactive micro- and nanoencapsulation strategies and their applications.

Valorization of jackfruit waste into value added products and their potential applications.

Jackfruit is a potential natural resource for many valuable biomaterials. The wastes from jackfruit are rich in carbohydrate, proteins, fats and phytochemicals. These wastes can be used as feedstock for the development of various bioproducts. The pretreatment strategies like biological, physical and chemical methods are being used for effective valorization of fruit wastes into value added products, like bioethanol, biogas, bioplastics, feeds, functional food additives, and other useful compounds. Bioenergy production from such renewable resources is an eco-friendly and cost-effective alternative option of fuels, unlike fossil fuels. The efficient bioconversion of fruit waste into useful biomaterials is facilitated by microbial fermentation process. Also, jackfruit peel is applied in the pollution abatement by remediation of dyes color from contaminated aquatic environment. Such technology can be used to develop a green economic model for waste utilization. This review addressed the utilization feasibility of jackfruit waste to produce value added products in order to reduce wastes and protect environment in a sustainable way.

Comparison of diversity and zinc solubilizing efficiency of rhizobacteria obtained from solanaceous crops under polyhouse and open field conditions.

Zinc-solubilizing bacteria (Zn-SB) play a crucial role in regulating soil fertility and plant health by maintaining Zn availability in the rhizosphere. It is uncertain how the Zn-SB population fluctuates across various cultivation systems since varied land-use patterns for agricultural aims may affect microbial activity and plant development effectiveness. The current study aims to examine the Zn-SB potential of various farming systems using Solanum lycopersicum, Solanum melongena, and Capsicum annuum grown in polyhouse soil (PS) and open fields (OF). Only twenty rhizobacterial isolates from PS and two isolates from OF out of 80 showed a strong ability to solubilize Zn, which was evaluated using Atomic Absorption Spectroscopy. Bacterial strain-PS4 solubilized 253.06 ppm of ZnO and produced a high quantity of lactic acid (168.62 g/ml) and acetic acid (470.5 g/ml), whereas bacterial strain OF1 solubilized 16.02 ppm of ZnO by releasing glycolic acid (42.89 g/ml), lactic acid (22.30 g/ml), formic acid (106.03 g/ml), and acetic acid (48.5 µg/ml). Further, in vitro studies demonstrated higher production of auxin, gibberellic acid and siderophore by PS1 as compared to OF1 strain. A large diversity of Zn-SB in the soil was indicated by biochemical analysis, which revealed that isolates belonged to the families Enterobacteriaceae, Bacillaceae, Burkholderiaceae, Streptococcaceae, Paenibacillaceae, Micrococcaceae, Morganellaceae, and Dietziaceae. The isolates PS4 and OF1 were identified as Bacillus cereus and Enterobacter hormaechei, respectively, using 16S rRNA sequencing. The findings show that soil from polyhouses has a greater diversity of Zn-solubilization rhizobacteria than soil from open areas. The findings suggested a potential land-use method for enhancing crop yields by employing microorganisms and polyhouse technology, which could be useful in the future study.

Understanding the salinity stress on plant and developing sustainable management strategies mediated salt-tolerant plant growth-promoting rhizobacteria and CRISPR/Cas9.

Soil salinity is a worldwide concern that decreases plant growth performance in agricultural fields and contributes to food scarcity. Salt stressors have adverse impacts on the plant's ionic, osmotic, and oxidative balance, as well as numerous physiological functions. Plants have a variety of coping strategies to deal with salt stress, including osmosensing, osmoregulation, ion-homeostasis, increased antioxidant synthesis, and so on. Not only does salt stress cause oxidative stress but also many types of stress do as well, thus plants have an effective antioxidant system to battle the negative effects of excessive reactive oxygen species produced as a result of stress. Rising salinity in the agricultural field affects crop productivity and plant development considerably; nevertheless, plants have a well-known copying mechanism that shields them from salt stress by facilitated production of secondary metabolites, antioxidants, ionhomeostasis, ABAbiosynthesis, and so on. To address this problem, various environment-friendly solutions such as salt-tolerant plant growth-promoting rhizobacteria, eco-friendly additives, and foliar applications of osmoprotectants/antioxidants are urgently needed. CRISPR/Cas9, a new genetic scissor, has recently been discovered to be an efficient approach for reducing salt stress in plants growing in saline soil. Understanding the processes underlying these physiological and biochemical responses to salt stress might lead to more effective crop yield control measures in the future. In order to address this information, the current review discusses recent advances in plant stress mechanisms against salinity stress-mediated antioxidant systems, as well as the development of appropriate long-term strategies for plant growth mediated by CRISPR/Cas9 techniques under salinity stress.

Biopolymeric nanoparticles based effective delivery of bioactive compounds toward the sustainable development of anticancerous therapeutics.

Nowadays, effective cancer therapy is a global concern, and recent advances in nanomedicine are crucial. Cancer is one of the major fatal diseases and a leading cause of death globally. Nanotechnology provides rapidly evolving delivery systems in science for treating diseases in a site-specific manner using natural bioactive compounds, which are gaining widespread attention. Nanotechnology combined with bioactives is a very appealing and relatively new area in cancer treatment. Natural bioactive compounds have the potential to be employed as a chemotherapeutic agent in the treatment of cancer, in addition to their nutritional benefits. Alginate, pullulan, cellulose, polylactic acid, chitosan, and other biopolymers have been effectively used in the delivery of therapeutics to a specific site. Because of their biodegradability, biopolymeric nanoparticles (BNPs) have received a lot of attention in the development of new anticancer drug delivery systems. Biopolymer-based nanoparticle systems can be made in a variety of ways. These systems have developed as a cost-effective and environmentally friendly solution to boost treatment efficacy. Effective drug delivery systems with improved availability, increased selectivity, and lower toxicity are needed. Recent research findings and current knowledge on the use of BNPs in the administration of bioactive chemicals in cancer therapy are summarized in this review.

Recent Strategies for Bioremediation of Emerging Pollutants: A Review for a Green and Sustainable Environment.

Environmental pollution brought on by xenobiotics and other related recalcitrant compounds have recently been identified as a major risk to both human health and the natural environment. Due to their toxicity and non-biodegradability, a wide range of pollutants, such as heavy metals, polychlorinated biphenyls, plastics, and various agrochemicals are present in the environment. Bioremediation is an effective cleaning technique for removing toxic waste from polluted environments that is gaining popularity. Various microorganisms, including aerobes and anaerobes, are used in bioremediation to treat contaminated sites. Microorganisms play a major role in bioremediation, given that it is a process in which hazardous wastes and pollutants are eliminated, degraded, detoxified, and immobilized. Pollutants are degraded and converted to less toxic forms, which is a primary goal of bioremediation. Ex situ or in situ bioremediation can be used, depending on a variety of factors, such as cost, pollutant types, and concentration. As a result, a suitable bioremediation method has been chosen. This review focuses on the most recent developments in bioremediation techniques, how microorganisms break down different pollutants, and what the future holds for bioremediation in order to reduce the amount of pollution in the world.

Microbial biofilms: Recent advances and progress in environmental bioremediation.

Microbial biofilms are formed by adherence of the bacteria through their secreted polymer matrices. The major constituents of the polymer matrices are extracellular DNAs, proteins, polysaccharides. Biofilms have exhibited a promising role in the area of bioremediation. These activities can be further improved by tuning the parameters like quorum sensing, characteristics of the adhesion surface, and other environmental factors. Organic pollutants have created a global concern because of their long-term toxicity on human, marine, and animal life. These contaminants are not easily degradable and continue to prevail in the environment for an extended period. Biofilms are being used for the remediation of different pollutants, among which organic pollutants have been of significance. The bioremediation of organic contaminants using biofilms is an eco-friendly, cheap, and green process. However, the development of this technology demands knowledge on the mechanism of action of the microbes to form the biofilm, types of specific bacteria or fungi responsible for the degradation of a particular organic compound, and the mechanistic role of the biofilm in the degradation of the pollutants. This review puts forth a comprehensive summary of the role of microbial biofilms in the bioremediation of different environment-threatening organic pollutants.

Valorization of sugar beet pulp to value-added products: A review.

The processing of sugar beet in the sugar production industry releases huge amounts of sugar beet pulp as waste which can be considered a valuable by-product as a source of cellulose, hemicellulose, and pectin. Valorization of sugar beet pulp into value added products occurs through acid hydrolysis, hydrothermal techniques, and enzymatic hydrolysis. Biochemical conversion of beet pulp into simple fermentable sugars for producing value added products occurs through enzymatic hydrolysis is a cost effective and eco-friendly process. While beet pulp has predominantly been used as a fodder for livestock, recent developments in its biotechnological valorization have unlocked its value as a feedstock in the production of biofuels, biohydrogen, biodegradable plastics, and platform chemicals such as lactic acid, citric acid, alcohols, microbial enzymes, single cell proteins, and pectic oligosaccharides. This review brings forward recent biotechnological developments made in the valorization of sugar beet pulp into valuable products.

Dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of Cr6+ from real tannery effluent employing indigenous Bacillus cereus isolate.

A native Bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and Cr(6+) remediation from the real tannery effluent. Most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. The extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with B. cereus isolate. Maximum growth, effluent decolorization (42.5 %), dechlorination (74.1 %), and Cr(6+) remediation (34.2 %) were attained with 4.0 % (v/v) inoculum, 0.8 % glucose, and 0.2 % ammonium chloride in 3:1 diluted effluent at natural pH (8.1) within 72 h of incubation. The efficiency of bioremediation in a bioreactor was higher as compared to a flask trial during 72 h of incubation: decolorization (47.9 %) was enhanced by 5.4 %, dechlorination (77.4 %) by 3.3 %, and Cr(6+) removal (41.7 %) by 7.5 % at an initial color of 286 Pt-Co units and initial concentration of 62 mg chloride ions and 108 mg l(-1) Cr(6+). Immobilized biomass of Pseudomonas putida and B. cereus coculture enhanced the extent of Cr(6+) remediation (51.9 %) by 10.2 % compared to the bioreactor trial. Chromate reductase activity and reduced Cr directly correlated and were mainly associated with soluble fraction of B. cereus plus effluent natural microflora. The GC-MS analyses revealed the formation of metabolites such as acetic acid and 2-butenoic acid in bacterially treated effluent. The supplementation of nutrients along with B. cereus augmentation is required for efficient effluent bioremediation.